{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:16:29Z","timestamp":1790381789336,"version":"4.1.0"},"reference-count":27,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T00:00:00Z","timestamp":1713916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"NIH grant","doi-asserted-by":"publisher","award":["R01AI155954"],"award-info":[{"award-number":["R01AI155954"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH grant","doi-asserted-by":"publisher","award":["R01AI132140"],"award-info":[{"award-number":["R01AI132140"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Valley Fever (VF), caused by fungi in the genus Coccidioides, is a prevalent disease in southwestern and western parts of the United States that affects both humans and animals, such as dogs. Although the immune responses to infection with Coccidioides spp. are not fully characterized, antibody-detection assays are used in conjunction with clinical presentation and radiologic findings to aid in the diagnosis of VF. These assays often use Complement Fixation (CF) and Tube Precipitin (TP) antigens as the main targets of IgG and IgM reactivity, respectively. Our group previously reported evidence of over 800 genes expressed at the protein level in C. posadasii. However, antibody reactivity to the majority of these proteins has never been explored. Using a new, high-throughput screening technology, the Nucleic Acid Programmable Protein Array (NAPPA), we screened serum specimens from dogs against 708 of these previously identified proteins for IgG reactivity. Serum from three separate groups of dogs was analyzed and revealed a small panel of proteins to be further characterized for immuno-reactivity. In addition to CF\/CTS1 antigen, sera from most infected dogs showed antibody reactivity to endo-1,3-betaglucanase, peroxisomal matrix protein, and another novel reactive protein, CPSG_05795. These antigens may provide additional targets to aid in antibody-based diagnostics.<\/jats:p>","DOI":"10.3390\/jof10050307","type":"journal-article","created":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T03:56:31Z","timestamp":1713930991000},"page":"307","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Discovery of a Unique Set of Dog-Seroreactive Coccidioides Proteins Using Nucleic Acid Programmable Protein Array"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3921-237X","authenticated-orcid":false,"given":"Megan A.","family":"Koehler","sequence":"first","affiliation":[{"name":"School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lusheng","family":"Song","sequence":"additional","affiliation":[{"name":"Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisca J.","family":"Grill","sequence":"additional","affiliation":[{"name":"Cactus Bio, LLC, Phoenix, AZ 85259, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa F.","family":"Shubitz","sequence":"additional","affiliation":[{"name":"Valley Fever Center for Excellence, The University of Arizona, Tucson, AZ 85724, USA"},{"name":"BIO5 Institute, The University of Arizona, Tucson, AZ 85724, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel A.","family":"Powell","sequence":"additional","affiliation":[{"name":"Valley Fever Center for Excellence, The University of Arizona, Tucson, AZ 85724, USA"},{"name":"BIO5 Institute, The University of Arizona, Tucson, AZ 85724, USA"},{"name":"Department of Immunobiology, The University of Arizona, Tucson, AZ 85724, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John N.","family":"Galgiani","sequence":"additional","affiliation":[{"name":"Valley Fever Center for Excellence, The University of Arizona, Tucson, AZ 85724, USA"},{"name":"BIO5 Institute, The University of Arizona, Tucson, AZ 85724, USA"},{"name":"Department of Immunobiology, The University of Arizona, Tucson, AZ 85724, USA"},{"name":"Department of Medicine, The University of Arizona, Tucson, AZ 85724, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7714-1352","authenticated-orcid":false,"given":"Marc J.","family":"Orbach","sequence":"additional","affiliation":[{"name":"Valley Fever Center for Excellence, The University of Arizona, Tucson, AZ 85724, USA"},{"name":"School of Plant Sciences, The University of Arizona, Tucson, AZ 85724, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edward J.","family":"Robb","sequence":"additional","affiliation":[{"name":"Anivive Life Sciences, LLC, Long Beach, CA 90807, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yunro","family":"Chung","sequence":"additional","affiliation":[{"name":"Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"},{"name":"College of Health Solutions, Arizona State University, Phoenix, AZ 85004, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stacy A.","family":"Williams","sequence":"additional","affiliation":[{"name":"Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7368-7742","authenticated-orcid":false,"given":"Vel","family":"Murugan","sequence":"additional","affiliation":[{"name":"Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin-gyoon","family":"Park","sequence":"additional","affiliation":[{"name":"Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joshua","family":"LaBaer","sequence":"additional","affiliation":[{"name":"Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Douglas F.","family":"Lake","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA"},{"name":"Cactus Bio, LLC, Phoenix, AZ 85259, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"D. Mitchell","family":"Magee","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Arizona State University, Tempe, AZ 85287, USA"},{"name":"Biodesign Institute, Arizona State University, Tempe, AZ 85287, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"536","DOI":"10.1056\/NEJMra2306821","article-title":"Coccidioidomycosis and Histoplasmosis in Immunocompetent Persons","volume":"390","author":"Galgiani","year":"2024","journal-title":"N. Engl. J. 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