{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,28]],"date-time":"2026-04-28T16:17:25Z","timestamp":1777393045457,"version":"3.51.4"},"reference-count":66,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T00:00:00Z","timestamp":1713916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK132778"],"award-info":[{"award-number":["DK132778"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["S10OD030223"],"award-info":[{"award-number":["S10OD030223"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["TL1TR002491"],"award-info":[{"award-number":["TL1TR002491"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["ECCS-2025064"],"award-info":[{"award-number":["ECCS-2025064"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Institutes of Health, National Center for Advancing Translational Sciences Program","award":["DK132778"],"award-info":[{"award-number":["DK132778"]}]},{"name":"National Institutes of Health, National Center for Advancing Translational Sciences Program","award":["S10OD030223"],"award-info":[{"award-number":["S10OD030223"]}]},{"name":"National Institutes of Health, National Center for Advancing Translational Sciences Program","award":["TL1TR002491"],"award-info":[{"award-number":["TL1TR002491"]}]},{"name":"National Institutes of Health, National Center for Advancing Translational Sciences Program","award":["ECCS-2025064"],"award-info":[{"award-number":["ECCS-2025064"]}]},{"name":"National Science Foundation","award":["DK132778"],"award-info":[{"award-number":["DK132778"]}]},{"name":"National Science Foundation","award":["S10OD030223"],"award-info":[{"award-number":["S10OD030223"]}]},{"name":"National Science Foundation","award":["TL1TR002491"],"award-info":[{"award-number":["TL1TR002491"]}]},{"name":"National Science Foundation","award":["ECCS-2025064"],"award-info":[{"award-number":["ECCS-2025064"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Pathogenic fungi are an increasing health threat due to the rise in drug resistance. The limited number of antifungals currently available and growing incidence of multi-drug-resistant fungi has caused rising healthcare costs and a decreased quality of life for patients with fungal infections. Nitric oxide (NO) has previously been shown to act as an antimicrobial agent, albeit with a limited understanding of the effects of the NO-release kinetics against pathogenic fungi. Herein, the antifungal effects of four nitric oxide-releasing small molecules were studied against the pathogenic fungi Candida albicans, Candida auris, Cryptococcus neoformans, and Aspergillus fumigatus, to demonstrate the broad-spectrum antifungal activity of NO. A bolus dose of NO was found to eradicate fungi after 24 h, where nitric oxide donors with shorter half-lives achieved antifungal activity at lower concentrations and thus had wider selectivity indexes. Each NO donor was found to cause a severe surface destruction of fungi, and all NO donors exhibited compatibility with currently prescribed antifungals against several different fungi species.<\/jats:p>","DOI":"10.3390\/jof10050308","type":"journal-article","created":{"date-parts":[[2024,4,24]],"date-time":"2024-04-24T07:38:51Z","timestamp":1713944331000},"page":"308","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Impact of Nitric Oxide-Release Kinetics on Antifungal Activity"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-9381-8551","authenticated-orcid":false,"given":"Quincy E.","family":"Grayton","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ivie L.","family":"Conlon","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christopher A.","family":"Broberg","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2212-0658","authenticated-orcid":false,"given":"Mark H.","family":"Schoenfisch","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"},{"name":"Eshelman School of Pharmacy, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"869","DOI":"10.4155\/fmc-2018-0465","article-title":"Targeting the Fungal Cell Wall: Current Therapies and Implications for Development of Alternative Antifungal Agents","volume":"11","author":"Hasim","year":"2019","journal-title":"Future Med. 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