{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T13:43:15Z","timestamp":1788961395555,"version":"build-2803163510"},"reference-count":46,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T00:00:00Z","timestamp":1714089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"the Anhui Provincial Natural Science Foundation","award":["1808085MC74"],"award-info":[{"award-number":["1808085MC74"]}]},{"name":"the Anhui Provincial Natural Science Foundation","award":["KJ2018A0676"],"award-info":[{"award-number":["KJ2018A0676"]}]},{"name":"the Anhui Provincial Natural Science Foundation","award":["gxgnfx2021118"],"award-info":[{"award-number":["gxgnfx2021118"]}]},{"name":"the Anhui Provincial Natural Science Foundation","award":["2023AH010045"],"award-info":[{"award-number":["2023AH010045"]}]},{"name":"University Natural Science Research Key projects in Anhui Province","award":["1808085MC74"],"award-info":[{"award-number":["1808085MC74"]}]},{"name":"University Natural Science Research Key projects in Anhui Province","award":["KJ2018A0676"],"award-info":[{"award-number":["KJ2018A0676"]}]},{"name":"University Natural Science Research Key projects in Anhui Province","award":["gxgnfx2021118"],"award-info":[{"award-number":["gxgnfx2021118"]}]},{"name":"University Natural Science Research Key projects in Anhui Province","award":["2023AH010045"],"award-info":[{"award-number":["2023AH010045"]}]},{"name":"Anhui Province University Outstanding Talents Cultivation Funding Project","award":["1808085MC74"],"award-info":[{"award-number":["1808085MC74"]}]},{"name":"Anhui Province University Outstanding Talents Cultivation Funding Project","award":["KJ2018A0676"],"award-info":[{"award-number":["KJ2018A0676"]}]},{"name":"Anhui Province University Outstanding Talents Cultivation Funding Project","award":["gxgnfx2021118"],"award-info":[{"award-number":["gxgnfx2021118"]}]},{"name":"Anhui Province University Outstanding Talents Cultivation Funding Project","award":["2023AH010045"],"award-info":[{"award-number":["2023AH010045"]}]},{"name":"Anhui Province University Innovation Team Project","award":["1808085MC74"],"award-info":[{"award-number":["1808085MC74"]}]},{"name":"Anhui Province University Innovation Team Project","award":["KJ2018A0676"],"award-info":[{"award-number":["KJ2018A0676"]}]},{"name":"Anhui Province University Innovation Team Project","award":["gxgnfx2021118"],"award-info":[{"award-number":["gxgnfx2021118"]}]},{"name":"Anhui Province University Innovation Team Project","award":["2023AH010045"],"award-info":[{"award-number":["2023AH010045"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Candida albicans (Ca), a prominent opportunistic fungal pathogen in humans, has garnered considerable attention due to its infectious properties. Herein, we have identified and characterized CaCDAP1 (Ca\u00a0orf19.1034), a homolog of ScDAP1 found in Saccharomyces cerevisiae. CaCDAP1 encodes a 183-amino acid protein with a conserved cytochrome b5-like heme-binding domain. The deletion of CaDAP1 renders Ca cells susceptible to caspofungin and terbinafine. CaDAP1 deletion confers resistance to Congo Red and Calcofluor White, and sensitivity to sodium dodecyl sulfate. The deletion of CaDAP1 results in a 50% reduction in chitin content within the cell wall, the downregulation of phosphorylation levels in CaMkc1, and the upregulation of phosphorylation levels in CaCek1. Notably, CaDAP1 deletion results in the abnormal hyphal development of Ca cells and diminishes virulence in a mouse systemic infection model. Thus, CaDAP1 emerges as a critical regulator governing cellular responses to antifungal drugs, the synthesis of cell wall chitin, and virulence in Ca.<\/jats:p>","DOI":"10.3390\/jof10050316","type":"journal-article","created":{"date-parts":[[2024,4,26]],"date-time":"2024-04-26T10:56:32Z","timestamp":1714128992000},"page":"316","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The Putative Cytochrome b5 Domain-Containing Protein CaDap1 Homologue Is Involved in Antifungal Drug Tolerance, Cell Wall Chitin Maintenance, and Virulence in Candida albicans"],"prefix":"10.3390","volume":"10","author":[{"given":"Dayong","family":"Xu","sequence":"first","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Manman","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xing","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongchen","family":"Mao","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haitao","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Biao","family":"Zhang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin","family":"Zeng","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Li","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Huaibei Normal University, Huaibei 235000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,4,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"112","DOI":"10.1038\/nrmicro2711","article-title":"Candida albicans morphogenesis and host defence: Discriminating invasion from colonization","volume":"10","author":"Gow","year":"2012","journal-title":"Nat. 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