{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T18:54:45Z","timestamp":1789066485914,"version":"build-2803163510"},"reference-count":45,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T00:00:00Z","timestamp":1717027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["823714322"],"award-info":[{"award-number":["823714322"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2023AH050727"],"award-info":[{"award-number":["2023AH050727"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["2023AH040114"],"award-info":[{"award-number":["2023AH040114"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["S202310369144"],"award-info":[{"award-number":["S202310369144"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Natural Science Foundation (Key project) of the University in Anhui Province","award":["823714322"],"award-info":[{"award-number":["823714322"]}]},{"name":"Natural Science Foundation (Key project) of the University in Anhui Province","award":["2023AH050727"],"award-info":[{"award-number":["2023AH050727"]}]},{"name":"Natural Science Foundation (Key project) of the University in Anhui Province","award":["2023AH040114"],"award-info":[{"award-number":["2023AH040114"]}]},{"name":"Natural Science Foundation (Key project) of the University in Anhui Province","award":["S202310369144"],"award-info":[{"award-number":["S202310369144"]}]},{"name":"Innovation and Entrepreneurship Project for College Students in Anhui Province","award":["823714322"],"award-info":[{"award-number":["823714322"]}]},{"name":"Innovation and Entrepreneurship Project for College Students in Anhui Province","award":["2023AH050727"],"award-info":[{"award-number":["2023AH050727"]}]},{"name":"Innovation and Entrepreneurship Project for College Students in Anhui Province","award":["2023AH040114"],"award-info":[{"award-number":["2023AH040114"]}]},{"name":"Innovation and Entrepreneurship Project for College Students in Anhui Province","award":["S202310369144"],"award-info":[{"award-number":["S202310369144"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Mitochondria, as the core metabolic organelles, play a crucial role in aerobic respiration\/biosynthesis in fungi. Numerous studies have demonstrated a close relationship between mitochondria and Candida albicans virulence and drug resistance. Here, we report an octapeptide-aminopeptidase located in the mitochondrial matrix named Oct1p. Its homolog in the model fungus Saccharomyces cerevisiae is one of the key proteins in maintaining mitochondrial respiration and protein stability. In this study, we utilized evolutionary tree analysis, gene knockout experiments, mitochondrial function detection, and other methods to demonstrate the impact of Oct1p on the mitochondrial function of C. albicans. Furthermore, through transcriptome analysis, real-time quantitative PCR, and morphological observation, we discovered that the absence of Oct1p results in functional abnormalities in C. albicans, affecting hyphal growth, cell adhesion, and biofilm formation. Finally, the in vivo results of the infection of Galleria mellonella larvae and vulvovaginal candidiasis in mice indicate that the loss of Oct1p led to the decreased virulence of C. albicans. In conclusion, this study provides a solid theoretical foundation for treating Candida diseases, developing new targeted drugs, and serves as a valuable reference for investigating the connection between mitochondria and virulence in other pathogenic fungi.<\/jats:p>","DOI":"10.3390\/jof10060391","type":"journal-article","created":{"date-parts":[[2024,5,30]],"date-time":"2024-05-30T08:15:54Z","timestamp":1717056954000},"page":"391","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Mitochondrial Protease Oct1p Regulates Mitochondrial Homeostasis and Influences Pathogenicity through Affecting Hyphal Growth and Biofilm Formation Activities in Candida albicans"],"prefix":"10.3390","volume":"10","author":[{"given":"Xiaoxiao","family":"Zhu","sequence":"first","affiliation":[{"name":"Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, 350 Longzihu Road, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feng","family":"Jin","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, 350 Longzihu Road, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guangyuan","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, 350 Longzihu Road, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tian","family":"Zhuang","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, 350 Longzihu Road, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cangcang","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, 350 Longzihu Road, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanjing","family":"Zhou","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, 350 Longzihu Road, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-7828-1509","authenticated-orcid":false,"given":"Xiaojia","family":"Niu","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, 350 Longzihu Road, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongchen","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, 350 Longzihu Road, Hefei 230012, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1581-1538","authenticated-orcid":false,"given":"Daqiang","family":"Wu","sequence":"additional","affiliation":[{"name":"Department of Pathogenic Biology and Immunology, College of Integrated Chinese and Western Medicine, Anhui University of Chinese Medicine, 350 Longzihu Road, Hefei 230012, China"},{"name":"Key Laboratory of Xin\u2019an Medicine, Ministry of Education, College of Nursing, Anhui University of Chinese Medicine, 350 Longzihu Road, Hefei 230038, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,5,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"929","DOI":"10.1038\/nature09486","article-title":"The energetics of genome complexity","volume":"467","author":"Lane","year":"2010","journal-title":"Nature"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Zeng, L.B., Huang, Y.C., Tan, J.J., Peng, J., Hu, N.Y., Liu, Q., Cao, Y.L., Zhang, Y.P., Chen, J.Z., and Huang, X.T. (2023). QCR7 affects the virulence of Candida albicans and the uptake of multiple carbon sources present in different host niches. Front. Cell. Infect. Microbiol., 13.","DOI":"10.3389\/fcimb.2023.1136698"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"ftw018","DOI":"10.1093\/femspd\/ftw018","article-title":"Pathogenesis of Candida albicans biofilm","volume":"74","author":"Tsui","year":"2016","journal-title":"Pathog. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"fov027","DOI":"10.1093\/femsyr\/fov027","article-title":"System-level impact of mitochondria on fungal virulence: To metabolism and beyond","volume":"15","author":"Calderone","year":"2015","journal-title":"FEMS Yeast Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1376","DOI":"10.1128\/EC.05184-11","article-title":"Mitochondria and Fungal Pathogenesis: Drug Tolerance, Virulence, and Potential for Antifungal Therapy","volume":"10","author":"Traven","year":"2011","journal-title":"Eukaryot. Cell"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"281","DOI":"10.1080\/21505594.2020.1870082","article-title":"MSS2 maintains mitochondrial function and is required for chitosan resistance, invasive growth, biofilm formation and virulence in Candida albicans","volume":"12","author":"Ke","year":"2021","journal-title":"Virulence"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.mib.2019.04.002","article-title":"Connecting iron regulation and mitochondrial function in Cryptococcus neoformans","volume":"52","author":"Horianopoulos","year":"2019","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"159","DOI":"10.1080\/21505594.2016.1188235","article-title":"Exploiting mitochondria as targets for the development of new antifungals","volume":"8","author":"Li","year":"2017","journal-title":"Virulence"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"930","DOI":"10.1111\/mmi.13167","article-title":"Mitochondrial dynamics in the pathogenic mold Aspergillus fumigatus: Therapeutic and evolutionary implications","volume":"98","author":"Neubauer","year":"2015","journal-title":"Mol. Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"292","DOI":"10.1016\/j.ejcb.2015.05.003","article-title":"Compartmentalization of iron between mitochondria and the cytosol and its regulation","volume":"94","author":"Hoffmann","year":"2015","journal-title":"Eur. J. Cell Biol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"426","DOI":"10.1080\/21505594.2017.1414133","article-title":"Exploring and exploiting the connection between mitochondria and the virulence of human pathogenic fungi","volume":"9","author":"Verma","year":"2018","journal-title":"Virulence"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"970","DOI":"10.1016\/j.molcel.2017.11.023","article-title":"Multi-omic Mitoprotease Profiling Defines a Role for Oct1p in Coenzyme Q Production","volume":"68","author":"Veling","year":"2017","journal-title":"Mol. Cell"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1083\/jcb.201308006","article-title":"The i-AAA protease YME1L and OMA1 cleave OPA1 to balance mitochondrial fusion and fission","volume":"204","author":"Anand","year":"2014","journal-title":"J. Cell Biol."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1038\/nrm3984","article-title":"New roles for mitochondrial proteases in health, ageing and disease","volume":"16","author":"Langer","year":"2015","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"2135","DOI":"10.1091\/mbc.e11-02-0169","article-title":"Mitochondrial protein turnover: Role of the precursor intermediate peptidase Oct1 in protein stabilization","volume":"22","author":"Prinz","year":"2011","journal-title":"Mol. Biol. Cell"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"17011","DOI":"10.1074\/jbc.M117.788588","article-title":"Proteolytic cleavage by the inner membrane peptidase (IMP) complex or Oct1 peptidase controls the localization of the yeast peroxiredoxin Prx1 to distinct mitochondrial compartments","volume":"292","author":"Gomes","year":"2017","journal-title":"J. Biol. Chem."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1128\/EC.4.2.298-309.2005","article-title":"Strains and strategies for large-scale gene deletion studies of the diploid human fungal pathogen Candida albicans","volume":"4","author":"Noble","year":"2005","journal-title":"Eukaryot. Cell"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1016\/j.fgb.2005.05.006","article-title":"Gene disruption in Candida albicans using a synthetic, codon-optimised Cre-loxP system","volume":"42","author":"Dennison","year":"2005","journal-title":"Fungal Genet. Biol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"4591134","DOI":"10.1155\/2022\/4591134","article-title":"Quercetin Protects Ethanol-Induced Hepatocyte Pyroptosis via Scavenging Mitochondrial ROS and Promoting PGC-1\u03b1-Regulated Mitochondrial Homeostasis in L02 Cells","volume":"2022","author":"Zhao","year":"2022","journal-title":"Oxidative Med. Cell. Longev."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"953","DOI":"10.1002\/iub.2476","article-title":"Naringin-generated ROS promotes mitochondria-mediated apoptosis in Candida albicans","volume":"73","author":"Kim","year":"2021","journal-title":"Iubmb Life"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1506","DOI":"10.1093\/emboj\/18.6.1506","article-title":"Sac1p plays a crucial role in microsomal ATP transport, which is distinct from its function in Golgi phospholipid metabolism","volume":"18","author":"Then","year":"1999","journal-title":"EMBO J."},{"key":"ref_22","first-page":"56","article-title":"Functional changes during apoptosis revealed by the simultaneous detection of mitochondrial membrane potential and DNA content by JC-1 and Hoechst 33342","volume":"59A","author":"Troiano","year":"2004","journal-title":"Cytom. Part A"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"jcs259980","DOI":"10.1242\/jcs.259980","article-title":"Loss of Num1-mediated cortical dynein anchoring negatively impacts respiratory growth","volume":"135","author":"White","year":"2022","journal-title":"J. Cell Sci."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Mahmoudabadi, A.Z., Zarrin, M., and Kiasat, N. (2014). Biofilm Formation and Susceptibility to Amphotericin B and Fluconazole in Candida albicans. Jundishapur J. Microbiol., 7.","DOI":"10.5812\/jjm.17105"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2253","DOI":"10.1038\/nprot.2006.397","article-title":"Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red","volume":"1","author":"Ram","year":"2006","journal-title":"Nat. Protoc."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Song, Y.J., Li, S.X., Zhao, Y.J., Zhang, Y.S., Lv, Y., Jiang, Y.Y., Wang, Y., Li, D.M., and Zhang, H. (2019). ADH1 promotes Candida albicans pathogenicity by stimulating oxidative phosphorylation. Int. J. Med. Microbiol., 309.","DOI":"10.1016\/j.ijmm.2019.151330"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e24888","DOI":"10.1002\/jcla.24888","article-title":"Phenotypes characterization and ABC genotypes distribution of clinical Candida albicans isolates","volume":"37","author":"Mahmoodi","year":"2023","journal-title":"J. Clin. Lab. Anal."},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Zhang, Q.Y., Xu, L., Yuan, S., Zhou, Q.H., Wang, X.X., Wang, L., Hu, Z.M., and Yan, Y.J. (2020). NGT1 Is Essential for N-Acetylglucosamine-Mediated Filamentous Growth Inhibition and HXK1 Functions as a Positive Regulator of Filamentous Growth in Candida tropicalis. Int. J. Mol. Sci., 21.","DOI":"10.3390\/ijms21114036"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1007\/s11046-010-9297-2","article-title":"An analysis of the Impact of NRG1 Overexpression on the Candida albicans Response to Specific Environmental Stimuli","volume":"170","author":"Cleary","year":"2010","journal-title":"Mycopathologia"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"1560","DOI":"10.1166\/sam.2023.4537","article-title":"Experimental Study on the Inhibition of Methicillin-Resistant Staphylococcus aureus Growth and Biofilm Formation by Berberine Hydrochloride Combined with Vancomycin","volume":"15","author":"Gan","year":"2023","journal-title":"Sci. Adv. Mater."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Liu, L.Y., Zhang, X.Y., Kayastha, S., Tan, L.H., Zhang, H., Tan, J.W., Li, L.Y., Mao, J.H., and Sun, Y. (2022). A Preliminary in vitro and in vivo Evaluation of the Effect and Action Mechanism of 17-AAG Combined with Azoles Against Azole-Resistant Candida spp.. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.825745"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"6179","DOI":"10.1128\/AAC.03046-15","article-title":"The Synergistic Effect of Azoles and Fluoxetine against Resistant Strains Is Attributed to Attenuating Fungal Virulence","volume":"60","author":"Gu","year":"2016","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"809","DOI":"10.2217\/fmb-2023-0033","article-title":"Extract of couplet medicines treats vulvovaginal candidiasis by affecting the vaginal mucosal barrier","volume":"18","author":"Zhang","year":"2023","journal-title":"Future Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"11247","DOI":"10.1038\/s41598-018-29423-5","article-title":"Estrogen Action in the Epithelial Cells of the Mouse Vagina Regulates Neutrophil Infiltration and Vaginal Tissue Integrity","volume":"8","author":"Li","year":"2018","journal-title":"Sci. Rep."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Liu, C., Xu, C., Du, Y., Liu, J., and Ning, Y. (2021). Role of agglutinin-like sequence protein 3 (Als3) in the structure and antifungal resistance of Candida albicans biofilms. FEMS Microbiol. Lett., 368.","DOI":"10.1093\/femsle\/fnab089"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1062","DOI":"10.1126\/science.288.5468.1062","article-title":"A high-affinity iron permease essential for Candida albicans virulence","volume":"288","author":"Ramanan","year":"2000","journal-title":"Science"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Martorano-Fernandes, L., Goodwine, J.G., Ricomini, A.P., Nobile, C.J., and Cury, A.A.D. (2023). Candida albicans Adhesins Als1 and Hwp1 Modulate Interactions with Streptococcus mutans. Microorganisms, 11.","DOI":"10.3390\/microorganisms11061391"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"295","DOI":"10.1038\/s41388-023-02907-z","article-title":"ROS production by mitochondria: Function or dysfunction?","volume":"43","author":"Palma","year":"2024","journal-title":"Oncogene"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Williams, R.B., and Lorenz, M.C. (2020). Multiple Alternative Carbon Pathways Combine to Promote Candida albicans Stress Resistance, Immune Interactions, and Virulence. mBio, 11.","DOI":"10.1128\/mBio.03070-19"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"280","DOI":"10.1128\/EC.00372-06","article-title":"Mutations in alternative carbon utilization pathways in Candida albicans attenuate virulence and confer pleiotropic phenotypes","volume":"6","author":"Lorenz","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"544","DOI":"10.3760\/cma.j.issn.0366-6999.2010.05.007","article-title":"Relationship between antifungal resistance of fluconazole resistant Candida albicans and mutations in ERG11 gene","volume":"123","author":"Feng","year":"2010","journal-title":"Chin. Med. J."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"105696","DOI":"10.1016\/j.micpath.2022.105696","article-title":"Molecular epidemiology, antifungal susceptibility, and ERG11 gene mutation of Candida species isolated from vulvovaginal candidiasis: Comparison between recurrent and non-recurrent infections","volume":"170","author":"Esfahani","year":"2022","journal-title":"Microb. Pathog."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"106166","DOI":"10.1016\/j.micpath.2023.106166","article-title":"Production of IgY against iron permease Ftr1 from Candida albicans and evaluation of its antifungal activity using Galleria mellonella as a model of systemic infection","volume":"181","author":"Gameiro","year":"2023","journal-title":"Microb. Pathog."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1016\/j.tim.2014.09.001","article-title":"Candida albicans hyphal initiation and elongation","volume":"22","author":"Lu","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Uppuluri, P., Chaturvedi, A.K., Srinivasan, A., Banerjee, M., Ramasubramaniam, A.K., K\u00f6hler, J.R., Kadosh, D., and Lopez-Ribot, J.L. (2010). Dispersion as an Important Step in the Candida albicans Biofilm Developmental Cycle. PLoS Pathog., 6.","DOI":"10.1371\/journal.ppat.1000828"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/10\/6\/391\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,10]],"date-time":"2025-10-10T14:51:05Z","timestamp":1760107865000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/10\/6\/391"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,30]]},"references-count":45,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2024,6]]}},"alternative-id":["jof10060391"],"URL":"https:\/\/doi.org\/10.3390\/jof10060391","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,30]]}}}