{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,16]],"date-time":"2026-09-16T19:51:45Z","timestamp":1789588305554,"version":"build-2803163510"},"reference-count":20,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2024,6,18]],"date-time":"2024-06-18T00:00:00Z","timestamp":1718668800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, Brazil","award":["409734\/2021-7"],"award-info":[{"award-number":["409734\/2021-7"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, Brazil","award":["APQ-1061-4.01\/21"],"award-info":[{"award-number":["APQ-1061-4.01\/21"]}]},{"name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico, Brazil","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Ci\u00eancia e Tecnologia do Estado de Pernambuco","award":["409734\/2021-7"],"award-info":[{"award-number":["409734\/2021-7"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Ci\u00eancia e Tecnologia do Estado de Pernambuco","award":["APQ-1061-4.01\/21"],"award-info":[{"award-number":["APQ-1061-4.01\/21"]}]},{"name":"Funda\u00e7\u00e3o de Amparo \u00e0 Ci\u00eancia e Tecnologia do Estado de Pernambuco","award":["001"],"award-info":[{"award-number":["001"]}]},{"name":"CAPES Finance","award":["409734\/2021-7"],"award-info":[{"award-number":["409734\/2021-7"]}]},{"name":"CAPES Finance","award":["APQ-1061-4.01\/21"],"award-info":[{"award-number":["APQ-1061-4.01\/21"]}]},{"name":"CAPES Finance","award":["001"],"award-info":[{"award-number":["001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The gold standard diagnosis of sporotrichosis is the isolation of Sporothrix sp. in culture media, but this is a time-consuming test that is susceptible to contamination and can be affected by the fungal load. Molecular methods such as nested PCR are gaining more ground in the management of several infections as they are tools for the rapid and accurate identification of microorganisms from pure cultures or directly from biological samples. This study aimed to apply a nested PCR molecular protocol for the rapid detection of Sporothrix spp. directly from clinical samples. Thirteen samples\u2014six from skin biopsies, five from skin exudates, and two from conjunctival secretions\u2014were obtained from patients diagnosed with sporotrichosis due to S. brasiliensis. Calmodulin gene sequencing identified all the isolates as S. brasiliensis. Nested PCR was able to detect all the Sporothrix sensu lato directly from clinical samples as well as the CBS 120339 reference strain. The nested PCR protocol stands out as a diagnostic alternative, as it allows the identification of Sporothrix spp. directly from clinical samples without the need for fungal isolation.<\/jats:p>","DOI":"10.3390\/jof10060432","type":"journal-article","created":{"date-parts":[[2024,6,19]],"date-time":"2024-06-19T08:06:06Z","timestamp":1718784366000},"page":"432","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Rapid Molecular Diagnosis of Sporotrichosis Directly from Biological Samples from a Reference Center in Brazil"],"prefix":"10.3390","volume":"10","author":[{"given":"Amanda Gabriela","family":"da Silva","sequence":"first","affiliation":[{"name":"Postgraduate Program in Tropical Medicine, Center for Medical Sciences, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil"},{"name":"Laboratory for Research and Diagnosis in Tropical Diseases, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5531-4963","authenticated-orcid":false,"given":"Arthur Felipe Cavalcanti","family":"de Matos","sequence":"additional","affiliation":[{"name":"Laboratory for Research and Diagnosis in Tropical Diseases, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bruna Rodrigues","family":"de Sousa","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Tropical Medicine, Center for Medical Sciences, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil"},{"name":"Laboratory for Research and Diagnosis in Tropical Diseases, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia Elise","family":"Ferraz","sequence":"additional","affiliation":[{"name":"Dermatology Reference Service, Hospital das Cl\u00ednicas, Federal University of Pernambuco (UFPE), Recife 50670-901, Pernambuco, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0620-0735","authenticated-orcid":false,"given":"Raul Leal Faria","family":"Luiz","sequence":"additional","affiliation":[{"name":"Oswaldo Cruz Institute, FIOCRUZ, Av Brasil, 4365, Manguinhos, Rio de Janeiro CEP 21045-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rejane Pereira","family":"Neves","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Tropical Medicine, Center for Medical Sciences, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil"},{"name":"Postgraduate Program in Fungal Biology, Biosciences Center, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8846-877X","authenticated-orcid":false,"given":"Reginaldo Gon\u00e7alves de","family":"Lima-Neto","sequence":"additional","affiliation":[{"name":"Postgraduate Program in Tropical Medicine, Center for Medical Sciences, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil"},{"name":"Laboratory for Research and Diagnosis in Tropical Diseases, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil"},{"name":"Dermatology Reference Service, Hospital das Cl\u00ednicas, Federal University of Pernambuco (UFPE), Recife 50670-901, Pernambuco, Brazil"},{"name":"Postgraduate Program in Fungal Biology, Biosciences Center, Federal University of Pernambuco (UFPE), Recife 50740-570, Pernambuco, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7670-9225","authenticated-orcid":false,"given":"Manoel Marques Evangelista","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Oswaldo Cruz Institute, FIOCRUZ, Av Brasil, 4365, Manguinhos, Rio de Janeiro CEP 21045-360, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"De Oliveira-Bento, A., de Sena Costa, A.S., Lima, S.L., do Monte Alves, M., de Azevedo Melo, A.S., Rodrigues, A.M., da Silva-Rocha, W.P., Milan, E.P., and Chaves, G.M. 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