{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,7]],"date-time":"2026-01-07T07:38:10Z","timestamp":1767771490508,"version":"build-2065373602"},"reference-count":41,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T00:00:00Z","timestamp":1719360000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program","doi-asserted-by":"publisher","award":["2023YFD1401100","31401453","CARS-01\u221202A"],"award-info":[{"award-number":["2023YFD1401100","31401453","CARS-01\u221202A"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"National Natural Science Foundation of China","award":["2023YFD1401100","31401453","CARS-01\u221202A"],"award-info":[{"award-number":["2023YFD1401100","31401453","CARS-01\u221202A"]}]},{"name":"China Academy of Agricultural Science","award":["2023YFD1401100","31401453","CARS-01\u221202A"],"award-info":[{"award-number":["2023YFD1401100","31401453","CARS-01\u221202A"]}]},{"name":"Agricultural Science and Technology Innovation Program of China Academy of Agricultural Science","award":["2023YFD1401100","31401453","CARS-01\u221202A"],"award-info":[{"award-number":["2023YFD1401100","31401453","CARS-01\u221202A"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Ophibolin A, a fungal sesterterpene, exerts a pivotal influence in a diverse array of biological processes, encompassing herbicidal, bactericidal, fungicidal, and cytotoxic activities. Sixty genes associated with sesterterpene compound biosynthesis were obtained from Bipolaris eleusines via transcriptome sequencing, and those closely linked to ophiobolin A biosynthesis were subsequently filtered. A gene encoding 3-hydroxy-3-methylglutaryl-CoA reductase (HMGR) that catalyzes the first committed step of ophiobolin biosynthesis in the mevalonic acid (MVA) pathway was isolated and characterized using RACE (Rapid Amplification of cDNA Ends) technology from ophiobolin A-producing fungus, B. eleusines. The full-length cDNA of the B. eleusines\u00a0HMGR gene (BeHMGR) was 3906 bp and contained a 3474 bp open reading frame (ORF) encoding 1157 amino acids. Sequence analysis revealed that deduced BeHMGR had high homology to the known HMGRs from Pyrenophora tritici-repentis and Leptosphaeria maculans. It had a calculated molecular mass of about 124.65 kDa and an isoelectric point (pI) of 6.90. It contained two putative HMG-CoA-binding motifs and two NADP(H)-binding motifs. Induced expression analysis of the BeHMGR gene by methyl jasmonate treatment using quantitative fluorescence PCR showed that it significantly elevated after 3 h of methyl jasmonate treatment, peaked at 6 h, and then gradually decreased. This demonstrates that BeHMGR gene expression is induced by methyl jasmonate.<\/jats:p>","DOI":"10.3390\/jof10070445","type":"journal-article","created":{"date-parts":[[2024,6,26]],"date-time":"2024-06-26T09:29:33Z","timestamp":1719394173000},"page":"445","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Molecular Characterization and Expression Analysis of a Gene Encoding 3-Hydroxy-3-Methylglutaryl-CoA Reductase (HMGR) from Bipolaris eleusines, an Ophiobolin A-Producing Fungus"],"prefix":"10.3390","volume":"10","author":[{"given":"Jianping","family":"Zhang","sequence":"first","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1934-5864","authenticated-orcid":false,"given":"Ke","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Industrial Engineering, University of Arkansas, Fayetteville, AR 72701, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6372-1102","authenticated-orcid":false,"given":"Wei","family":"Tang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongjie","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaoyue","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yongliang","family":"Lu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liuqing","family":"Yu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Rice Biology and Breeding, China National Rice Research Institute, Hangzhou 310006, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2024,6,26]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"839","DOI":"10.1080\/09583157.2014.891724","article-title":"Bipolaris eleusines, a potential mycoherbicide candidate for control of barnyardgrass (Echinochloa crus-galli)","volume":"24","author":"Zhang","year":"2014","journal-title":"Biocontrol. 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