{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:23:21Z","timestamp":1789431801402,"version":"build-2803163510"},"reference-count":96,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2024,7,20]],"date-time":"2024-07-20T00:00:00Z","timestamp":1721433600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Consejo Nacional de Humanidades, Ciencias y Tecnolog\u00edas","award":["Ciencia de Frontera 2019-6380"],"award-info":[{"award-number":["Ciencia de Frontera 2019-6380"]}]},{"name":"Consejo Nacional de Humanidades, Ciencias y Tecnolog\u00edas","award":["CBF2023-2024-655"],"award-info":[{"award-number":["CBF2023-2024-655"]}]},{"name":"Consejo Nacional de Humanidades, Ciencias y Tecnolog\u00edas","award":["CONACYT-M\u00e9xico"],"award-info":[{"award-number":["CONACYT-M\u00e9xico"]}]},{"name":"Red Tem\u00e1tica Glicociencia en Salud","award":["Ciencia de Frontera 2019-6380"],"award-info":[{"award-number":["Ciencia de Frontera 2019-6380"]}]},{"name":"Red Tem\u00e1tica Glicociencia en Salud","award":["CBF2023-2024-655"],"award-info":[{"award-number":["CBF2023-2024-655"]}]},{"name":"Red Tem\u00e1tica Glicociencia en Salud","award":["CONACYT-M\u00e9xico"],"award-info":[{"award-number":["CONACYT-M\u00e9xico"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Candidemia is an opportunistic mycosis with high morbidity and mortality rates. Even though Candida albicans is the main causative agent, other Candida species, such as Candida tropicalis, are relevant etiological agents of candidiasis and candidemia. Compared with C.\u00a0albicans, there is currently limited information about C.\u00a0tropicalis\u2019 biological aspects, including those related to the cell wall and the interaction with the host. Currently, it is known that its cell wall contains O-linked mannans, and the contribution of these structures to cell fitness has previously been addressed using cells subjected to chemical treatments or in mutants where O-linked mannans and other wall components are affected. Here, we generated a C.\u00a0tropicalis\u00a0pmt2\u2206 null mutant, which was affected in the first step of the O-linked mannosylation pathway. The null mutant was viable, contrasting with C.\u00a0albicans where this gene is essential. The phenotypical characterization showed that O-linked mannans were required for filamentation; proper cell wall integrity and organization; biofilm formation; protein secretion; and adhesion to extracellular matrix components, in particular to fibronectin; and type I and type II collagen. When interacting with human innate immune cells, it was found that this cell wall structure is dispensable for cytokine production, but mutant cells were more phagocytosed by monocyte-derived macrophages. Furthermore, the null mutant cells showed virulence attenuation in Galleria mellonella larvae. Thus, O-linked mannans are minor components of the cell wall that are involved in different aspects of C.\u00a0tropicalis\u2019 biology.<\/jats:p>","DOI":"10.3390\/jof10070502","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T17:36:04Z","timestamp":1721669764000},"page":"502","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Candida tropicalis PMT2 Is a Dispensable Gene for Viability but Required for Proper Interaction with the Host"],"prefix":"10.3390","volume":"10","author":[{"given":"Marco J.","family":"Hern\u00e1ndez-Ch\u00e1vez","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s\/n, col. Noria Alta, C.P., Guanajuato 36050, GTO, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5626-2892","authenticated-orcid":false,"given":"Iv\u00e1n","family":"Mart\u00ednez-Duncker","sequence":"additional","affiliation":[{"name":"Laboratorio de Glicobiolog\u00eda Humana y Diagn\u00f3stico Molecular, Centro de Investigaci\u00f3n en Din\u00e1mica Celular, Instituto de Investigaci\u00f3n en Ciencias B\u00e1sicas y Aplicadas, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca 62209, MOR, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Diana M.","family":"Clavijo-Giraldo","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s\/n, col. Noria Alta, C.P., Guanajuato 36050, GTO, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz A.","family":"L\u00f3pez-Ramirez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s\/n, col. Noria Alta, C.P., Guanajuato 36050, GTO, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6973-0595","authenticated-orcid":false,"given":"H\u00e9ctor M.","family":"Mora-Montes","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s\/n, col. Noria Alta, C.P., Guanajuato 36050, GTO, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2024,7,20]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"845","DOI":"10.1007\/s11046-023-00743-z","article-title":"Candida spp. in human intestinal health and disease: More than a gut feeling","volume":"188","author":"Kreulen","year":"2023","journal-title":"Mycopathologia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1007\/s10096-006-0159-2","article-title":"Attributable mortality of candidemia: A systematic review of matched cohort and case-control studies","volume":"25","author":"Falagas","year":"2006","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/s41572-024-00503-3","article-title":"Invasive candidiasis","volume":"10","author":"Kanj","year":"2024","journal-title":"Nat. Rev. Dis. 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