{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,25]],"date-time":"2026-02-25T06:07:50Z","timestamp":1771999670650,"version":"3.50.1"},"reference-count":35,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,10,26]],"date-time":"2024-10-26T00:00:00Z","timestamp":1729900800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Live-cell imaging generally requires pretreatment with fluorophores to either monitor cellular functions or the dynamics of intracellular processes and structures. We have recently introduced full-field optical coherence tomography for the label-free live-cell imaging of fungi with potential clinical applications for the diagnosis of invasive fungal mold infections. While both the spatial resolution and technical set up of this technology are more likely designed for the histopathological analysis of tissue biopsies, there is to our knowledge no previous work reporting the use of a light interference-based optical technique for direct mycological examination and monitoring of intracellular processes. We describe the first application of dynamic full-field optical transmission tomography (D-FF-OTT) to achieve both high-resolution and live-cell imaging of fungi. First, D-FF-OTT allowed for the precise examination and identification of several elementary structures within a selection of fungal species commonly known to be responsible for invasive fungal infections such as Candida albicans, Aspergillus fumigatus, or Rhizopus arrhizus. Furthermore, D-FF-OTT revealed the intracellular trafficking of organelles and vesicles related to metabolic processes of living fungi, thus opening new perspectives in fast fungal infection diagnostics.<\/jats:p>","DOI":"10.3390\/jof10110741","type":"journal-article","created":{"date-parts":[[2024,10,28]],"date-time":"2024-10-28T08:39:07Z","timestamp":1730104747000},"page":"741","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Label-Free Optical Transmission Tomography for Direct Mycological Examination and Monitoring of Intracellular Dynamics"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6264-0607","authenticated-orcid":false,"given":"Eliott","family":"Teston","sequence":"first","affiliation":[{"name":"Lipness Team, Translational Research Center in Molecular Medicine\u2013 INSERM Joint Research Unit (CTM-UMR1231), University of Burgundy, 21000 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marc","family":"Sautour","sequence":"additional","affiliation":[{"name":"Department of Parasitology\/Mycology, Dijon Bourgogne University Hospital, 21000 Dijon, France"},{"name":"Unit\u00e9 mixte de recherche Proc\u00e9d\u00e9s Alimentaires et Microbiologiques (UMR PAM) A 02.102, Bourgogne Franche-Comt\u00e9 University, AgroSup Dijon, 21079 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"L\u00e9a","family":"Boulnois","sequence":"additional","affiliation":[{"name":"Medical Biology Laboratory, William Morey General Hospital, 71100 Chalon-sur-Sa\u00f4ne, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nicolas","family":"Augey","sequence":"additional","affiliation":[{"name":"LISPEN, Arts et Metiers Institute of Technology, 71100 Chalon-sur-Sa\u00f4ne, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Abdellah","family":"Dighab","sequence":"additional","affiliation":[{"name":"LISPEN, Arts et Metiers Institute of Technology, 71100 Chalon-sur-Sa\u00f4ne, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8104-4600","authenticated-orcid":false,"given":"Christophe","family":"Guillet","sequence":"additional","affiliation":[{"name":"LISPEN, Arts et Metiers Institute of Technology, 71100 Chalon-sur-Sa\u00f4ne, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5288-2904","authenticated-orcid":false,"given":"Dea","family":"Garcia-Hermoso","sequence":"additional","affiliation":[{"name":"Translational Mycology Research Group, Mycology Department, National Reference Center for Invasive Mycoses and Antifungals, Institut Pasteur, Paris Cit\u00e9 University, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fanny","family":"Lanternier","sequence":"additional","affiliation":[{"name":"Translational Mycology Research Group, Mycology Department, National Reference Center for Invasive Mycoses and Antifungals, Institut Pasteur, Paris Cit\u00e9 University, 75015 Paris, France"},{"name":"Necker-Enfants Malades Hospital, Assistance Publique-H\u00f4pitaux de Paris, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Elisabeth","family":"Bougnoux","sequence":"additional","affiliation":[{"name":"Necker-Enfants Malades Hospital, Assistance Publique-H\u00f4pitaux de Paris, 75015 Paris, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fr\u00e9d\u00e9ric","family":"Dalle","sequence":"additional","affiliation":[{"name":"Department of Parasitology\/Mycology, Dijon Bourgogne University Hospital, 21000 Dijon, France"},{"name":"Unit\u00e9 mixte de recherche Proc\u00e9d\u00e9s Alimentaires et Microbiologiques (UMR PAM) A 02.102, Bourgogne Franche-Comt\u00e9 University, AgroSup Dijon, 21079 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4214-8022","authenticated-orcid":false,"given":"Louise","family":"Basmaciyan","sequence":"additional","affiliation":[{"name":"Department of Parasitology\/Mycology, Dijon Bourgogne University Hospital, 21000 Dijon, France"},{"name":"Unit\u00e9 mixte de recherche Proc\u00e9d\u00e9s Alimentaires et Microbiologiques (UMR PAM) A 02.102, Bourgogne Franche-Comt\u00e9 University, AgroSup Dijon, 21079 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mathieu","family":"Blot","sequence":"additional","affiliation":[{"name":"Lipness Team, Translational Research Center in Molecular Medicine\u2013 INSERM Joint Research Unit (CTM-UMR1231), University of Burgundy, 21000 Dijon, France"},{"name":"Infectious Diseases Department, Dijon Bourgogne University Hospital, 21000 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pierre-Emmanuel","family":"Charles","sequence":"additional","affiliation":[{"name":"Lipness Team, Translational Research Center in Molecular Medicine\u2013 INSERM Joint Research Unit (CTM-UMR1231), University of Burgundy, 21000 Dijon, France"},{"name":"Department of Intensive Care Medicine, Dijon Bourgogne University Hospital, 21000 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2351-682X","authenticated-orcid":false,"given":"Jean-Pierre","family":"Quenot","sequence":"additional","affiliation":[{"name":"Lipness Team, Translational Research Center in Molecular Medicine\u2013 INSERM Joint Research Unit (CTM-UMR1231), University of Burgundy, 21000 Dijon, France"},{"name":"Department of Intensive Care Medicine, Dijon Bourgogne University Hospital, 21000 Dijon, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bianca","family":"Podac","sequence":"additional","affiliation":[{"name":"Medical Biology Laboratory, William Morey General Hospital, 71100 Chalon-sur-Sa\u00f4ne, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0998-0669","authenticated-orcid":false,"given":"Catherine","family":"Neuwirth","sequence":"additional","affiliation":[{"name":"Department of Bacteriology, University Hospital of Dijon, 21000 Dijon, France"},{"name":"UMR\/CNRS 6248 Chrono-Environnement, Bougogne Franche-Comt\u00e9 University, 25000 Besan\u00e7on, France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Claude","family":"Boccara","sequence":"additional","affiliation":[{"name":"Institut Langevin, Ecole Sup\u00e9rieure de Physique et Chimie Industrielle de la ville de Paris, Universit\u00e9 PSL, CNRS, 75005 Paris, 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