{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,21]],"date-time":"2026-07-21T10:02:14Z","timestamp":1784628134773,"version":"3.55.0"},"reference-count":58,"publisher":"MDPI AG","issue":"11","license":[{"start":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T00:00:00Z","timestamp":1730419200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Science and Engineering Research Board","award":["CRG\/2022\/001047"],"award-info":[{"award-number":["CRG\/2022\/001047"]}]},{"name":"Science and Engineering Research Board","award":["R01 AI063503"],"award-info":[{"award-number":["R01 AI063503"]}]},{"name":"Science and Engineering Research Board","award":["G0L1622N"],"award-info":[{"award-number":["G0L1622N"]}]},{"name":"Science and Engineering Research Board","award":["C3\/22\/007"],"award-info":[{"award-number":["C3\/22\/007"]}]},{"name":"Government of India","award":["CRG\/2022\/001047"],"award-info":[{"award-number":["CRG\/2022\/001047"]}]},{"name":"Government of India","award":["R01 AI063503"],"award-info":[{"award-number":["R01 AI063503"]}]},{"name":"Government of India","award":["G0L1622N"],"award-info":[{"award-number":["G0L1622N"]}]},{"name":"Government of India","award":["C3\/22\/007"],"award-info":[{"award-number":["C3\/22\/007"]}]},{"name":"Boost to University Interdisciplinary Life Science Departments for Education and Research Program (DBT-Builder)","award":["CRG\/2022\/001047"],"award-info":[{"award-number":["CRG\/2022\/001047"]}]},{"name":"Boost to University Interdisciplinary Life Science Departments for Education and Research Program (DBT-Builder)","award":["R01 AI063503"],"award-info":[{"award-number":["R01 AI063503"]}]},{"name":"Boost to University Interdisciplinary Life Science Departments for Education and Research Program (DBT-Builder)","award":["G0L1622N"],"award-info":[{"award-number":["G0L1622N"]}]},{"name":"Boost to University Interdisciplinary Life Science Departments for Education and Research Program (DBT-Builder)","award":["C3\/22\/007"],"award-info":[{"award-number":["C3\/22\/007"]}]},{"name":"National Institutes of Allergy and Infectious Diseases","award":["CRG\/2022\/001047"],"award-info":[{"award-number":["CRG\/2022\/001047"]}]},{"name":"National Institutes of Allergy and Infectious Diseases","award":["R01 AI063503"],"award-info":[{"award-number":["R01 AI063503"]}]},{"name":"National Institutes of Allergy and Infectious Diseases","award":["G0L1622N"],"award-info":[{"award-number":["G0L1622N"]}]},{"name":"National Institutes of Allergy and Infectious Diseases","award":["C3\/22\/007"],"award-info":[{"award-number":["C3\/22\/007"]}]},{"name":"FWO","award":["CRG\/2022\/001047"],"award-info":[{"award-number":["CRG\/2022\/001047"]}]},{"name":"FWO","award":["R01 AI063503"],"award-info":[{"award-number":["R01 AI063503"]}]},{"name":"FWO","award":["G0L1622N"],"award-info":[{"award-number":["G0L1622N"]}]},{"name":"FWO","award":["C3\/22\/007"],"award-info":[{"award-number":["C3\/22\/007"]}]},{"name":"Industrial Research Fund","award":["CRG\/2022\/001047"],"award-info":[{"award-number":["CRG\/2022\/001047"]}]},{"name":"Industrial Research Fund","award":["R01 AI063503"],"award-info":[{"award-number":["R01 AI063503"]}]},{"name":"Industrial Research Fund","award":["G0L1622N"],"award-info":[{"award-number":["G0L1622N"]}]},{"name":"Industrial Research Fund","award":["C3\/22\/007"],"award-info":[{"award-number":["C3\/22\/007"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Certain species of Mucorales have been identified as causative agents of mucormycosis, a rare yet often lethal fungal infection. Notably, these fungi exhibit intrinsic resistance to common azole drugs, which target lipids. Given the pivotal role of lipids in drug resistance and their contribution to innate resistance to azoles, this study provides a comprehensive overview of key lipid classes, including sphingolipids (SLs), glycerophospholipids (GPLs), and sterols, in Rhizopus delemar 99-880, a well-characterized reference strain among Mucorales. Using shotgun lipidomics as well as liquid- and gas-chromatography-based mass spectrometric analyses, we identified the lipid intermediates and elucidated the biosynthetic pathways of SLs, PGLs, and sterols. The acidic SLs were not found, probably because the acidic branch of the SL biosynthesis pathway terminates at \u03b1-hydroxy phytoceramides, as evident by their high abundance. Intermediates in the neutral SL pathway incorporated higher levels of 16:0 fatty acid compared to other pathogenic fungi. A strikingly high phosphatidylethanolamine (PE)\/phosphatdylcholine (PC) ratio was observed among GPLs. Ergosterol remains the major sterol, similar to other fungi, and our analysis confirms the existence of alternate ergosterol biosynthesis pathways. The total lipidomic profile of R. delemar 99-880 offers insights into its lipid metabolism and potential implications for studying pathogenesis and drug resistance mechanisms.<\/jats:p>","DOI":"10.3390\/jof10110760","type":"journal-article","created":{"date-parts":[[2024,11,1]],"date-time":"2024-11-01T13:09:27Z","timestamp":1730466567000},"page":"760","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["A Comprehensive Analysis of the Lipidomic Signatures in Rhizopus delemar"],"prefix":"10.3390","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-8266-8605","authenticated-orcid":false,"given":"Basharat","family":"Ali","sequence":"first","affiliation":[{"name":"Amity Institute of Integrative Science and Health, Amity University Gurugram, Gurugram 122413, India"},{"name":"Amity Institute of Biotechnology, Amity University Gurugram, Gurugram 122413, India"},{"name":"School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Anshu","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Amity Institute of Integrative Science and Health, Amity University Gurugram, Gurugram 122413, India"},{"name":"Amity Institute of Biotechnology, Amity University Gurugram, Gurugram 122413, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohit","family":"Kumar","sequence":"additional","affiliation":[{"name":"Yeast Biofuel Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Praveen","family":"Kumar","sequence":"additional","affiliation":[{"name":"Amity Institute of Integrative Science and Health, Amity University Gurugram, Gurugram 122413, India"},{"name":"Amity Institute of Biotechnology, Amity University Gurugram, Gurugram 122413, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1507-3475","authenticated-orcid":false,"given":"Hans","family":"Carolus","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cell Biology, Department of Biology, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Celia","family":"Lobo Romero","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cell Biology, Department of Biology, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rudy","family":"Vergauwen","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cell Biology, Department of Biology, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0995-6696","authenticated-orcid":false,"given":"Ashutosh","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biochemistry, University of Lucknow, Lucknow 226007, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9319-4593","authenticated-orcid":false,"given":"Atanu","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Amity Institute of Integrative Science and Health, Amity University Gurugram, Gurugram 122413, India"},{"name":"Amity Institute of Biotechnology, Amity University Gurugram, Gurugram 122413, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amresh","family":"Prakash","sequence":"additional","affiliation":[{"name":"Amity Institute of Integrative Science and Health, Amity University Gurugram, Gurugram 122413, India"},{"name":"Amity Institute of Biotechnology, Amity University Gurugram, Gurugram 122413, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9097-9253","authenticated-orcid":false,"given":"Shivaprakash M.","family":"Rudramurthy","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, Postgraduate Institute of Medical Education & Research, Chandigarh 160012, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1542-897X","authenticated-orcid":false,"given":"Patrick","family":"Van Dijck","sequence":"additional","affiliation":[{"name":"Laboratory of Molecular Cell Biology, Department of Biology, KU Leuven, 3000 Leuven, Belgium"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3787-8530","authenticated-orcid":false,"given":"Ashraf S.","family":"Ibrahim","sequence":"additional","affiliation":[{"name":"Division of Infectious Diseases, The Lundquist Institute at 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