{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T18:42:09Z","timestamp":1782326529688,"version":"3.54.5"},"reference-count":92,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T00:00:00Z","timestamp":1737072000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"GORE-Coquimbo","award":["FIC-R 30485880-0"],"award-info":[{"award-number":["FIC-R 30485880-0"]}]},{"name":"GORE-Coquimbo","award":["PT17122"],"award-info":[{"award-number":["PT17122"]}]},{"name":"GORE-Coquimbo","award":["PT13121"],"award-info":[{"award-number":["PT13121"]}]},{"name":"GORE-Coquimbo","award":["2019-6380"],"award-info":[{"award-number":["2019-6380"]}]},{"name":"GORE-Coquimbo","award":["CBF2023-2024-655"],"award-info":[{"award-number":["CBF2023-2024-655"]}]},{"name":"Research Department of Universidad de La Serena [DIDULS]","award":["FIC-R 30485880-0"],"award-info":[{"award-number":["FIC-R 30485880-0"]}]},{"name":"Research Department of Universidad de La Serena [DIDULS]","award":["PT17122"],"award-info":[{"award-number":["PT17122"]}]},{"name":"Research Department of Universidad de La Serena [DIDULS]","award":["PT13121"],"award-info":[{"award-number":["PT13121"]}]},{"name":"Research Department of Universidad de La Serena [DIDULS]","award":["2019-6380"],"award-info":[{"award-number":["2019-6380"]}]},{"name":"Research Department of Universidad de La Serena [DIDULS]","award":["CBF2023-2024-655"],"award-info":[{"award-number":["CBF2023-2024-655"]}]},{"name":"CONAHCYT-Mexico","award":["FIC-R 30485880-0"],"award-info":[{"award-number":["FIC-R 30485880-0"]}]},{"name":"CONAHCYT-Mexico","award":["PT17122"],"award-info":[{"award-number":["PT17122"]}]},{"name":"CONAHCYT-Mexico","award":["PT13121"],"award-info":[{"award-number":["PT13121"]}]},{"name":"CONAHCYT-Mexico","award":["2019-6380"],"award-info":[{"award-number":["2019-6380"]}]},{"name":"CONAHCYT-Mexico","award":["CBF2023-2024-655"],"award-info":[{"award-number":["CBF2023-2024-655"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Proteins found within the fungal cell wall usually contain both N- and O-oligosaccharides. N-glycosylation is the process where these oligosaccharides (hereinafter: glycans) are attached to asparagine residues, while in O-glycosylation the glycans are covalently bound to serine or threonine residues. The PMT family is grouped into PMT1, PMT2, and PMT4 subfamilies. Using bioinformatics analysis within the Botrytis cinerea genome database, an ortholog to Saccharomyces cerevisiae Pmt4 and other fungal species was identified. The aim of this study was to assess the relevance of the bcpmt4 gene in B. cinerea glycosylation. For this purpose, the bcpmt4 gene was disrupted by homologous recombination in the B05.10 strain using a hygromycin B resistance cassette. Expression of bcpmt4 in S. cerevisiae \u0394Scpmt4 or \u0394Scpmt3 null mutants restored glycan levels like those observed in the parental strain. The phenotypic analysis showed that \u0394bcpmt4 null mutants exhibited significant changes in hyphal cell wall composition, including reduced mannan levels and increased amounts of chitin and glucan. Furthermore, the loss of bcpmt4 led to decreased glycosylation of glycoproteins in the B. cinerea cell wall. The null mutant lacking PMT4 was hypersensitive to a range of cell wall perturbing agents, antifungal drugs, and high hydrostatic pressure. Thus, in addition to their role in glycosylation, the PMT4 is required to virulence, biofilm formation, and membrane integrity. This study adds to our knowledge of the role of the B. cinerea\u00a0bcpmt4 gene, which is involved in glycosylation and cell biology, cell wall formation, and antifungal response.<\/jats:p>","DOI":"10.3390\/jof11010071","type":"journal-article","created":{"date-parts":[[2025,1,17]],"date-time":"2025-01-17T03:33:02Z","timestamp":1737084782000},"page":"71","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Botrytis cinerea PMT4 Is Involved in O-Glycosylation, Cell Wall Organization, Membrane Integrity, and Virulence"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5002-8309","authenticated-orcid":false,"given":"Ver\u00f3nica","family":"Plaza","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular y Bioqu\u00edmica, Departamento de Biolog\u00eda, Universidad de La Serena, La Serena 1700000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Pasten","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular y Bioqu\u00edmica, Departamento de Biolog\u00eda, Universidad de La Serena, La Serena 1700000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luz A.","family":"L\u00f3pez-Ram\u00edrez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato 36050, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6973-0595","authenticated-orcid":false,"given":"H\u00e9ctor M.","family":"Mora-Montes","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Universidad de Guanajuato, Guanajuato 36050, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Rubio-Astudillo","sequence":"additional","affiliation":[{"name":"Instituto de Ciencias Biom\u00e9dicas, Universidad Aut\u00f3noma de Chile, Santiago 7500912, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8745-7916","authenticated-orcid":false,"given":"Evelyn","family":"Silva-Moreno","sequence":"additional","affiliation":[{"name":"GEMA Center for Genomics, Ecology and Environment, Faculty of Interdisciplinary Studies, Universidad Mayor, Santiago 7510041, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1834-8390","authenticated-orcid":false,"given":"Luis","family":"Castillo","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular y Bioqu\u00edmica, Departamento de Biolog\u00eda, Universidad de La Serena, La Serena 1700000, Chile"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,1,17]]},"reference":[{"key":"ref_1","unstructured":"Elad, Y., Williamson, B., Tudzynski, P., and Delen, N. (2004). Botrytis spp. and diseases they cause in agricultural systems\u2014An introduction. Botrytis: Biology, Pathology and Control, Kluwer Academic Publishers."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Fillinger, S., and Elad, Y. (2016). Botrytis\u2014The Fungus, the Pathogen and Its Management in Agricultural Systems, Springer International Publishing.","DOI":"10.1007\/978-3-319-23371-0"},{"key":"ref_3","unstructured":"Jarvis, W. (1977). Botryotinia and Botrytis Species: Taxonomy, Physiology and Pathogenicity\u2014A Guide to the Literature, Agriculture Canada."},{"key":"ref_4","first-page":"100049","article-title":"Molecular identification and characterization of Botrytis cinerea associated to the endemic flora of semi-desert climate in Chile","volume":"2","author":"Notte","year":"2021","journal-title":"Curr. Res. Microb. Sci."},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Balasubramaniam, V.M., Barbosa-C\u00e1novas, G.V., and Lelieveld, H.L.M. (2016). Microbiological Aspects of High-Pressure Processing of Food: Inactivation of Microbial Vegetative Cells and Spores. High Pressure Processing of Food: Principles, Technology and Applications, Springer.","DOI":"10.1007\/978-1-4939-3234-4"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"De Simone, N., Pace, B., Grieco, F., Chimienti, M., Tyibilika, V., Santoro, V., Capozzi, V., Colelli, G., Spano, G., and Russo, P. (2020). Botrytis cinerea and Table Grapes: A Review of the Main Physical, Chemical, and Bio-Based Control Treatments in Post-Harvest. Foods, 9.","DOI":"10.3390\/foods9091138"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"55","DOI":"10.1016\/j.foodres.2016.11.029","article-title":"Effect of high hydrostatic pressure processing on the background microbial loads and quality of cantaloupe puree","volume":"91","author":"Mukhopadhyay","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"2029","DOI":"10.1099\/mic.0.26980-0","article-title":"Fungal cell wall chitinases and glucanases","volume":"150","author":"Adams","year":"2004","journal-title":"Microbiology"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"611","DOI":"10.1002\/yea.1790","article-title":"The Candida albicans cell wall protein Rhd3\/Pga29 is abundant in the yeast form and contributes to virulence","volume":"27","author":"Weindl","year":"2010","journal-title":"Yeast"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"2245","DOI":"10.1101\/gad.1121003","article-title":"Virulence-related surface glycoproteins in the yeast pathogen Candida glabrata are encoded in subtelomeric clusters and subject to RAP1- and SIR-dependent transcriptional silencing","volume":"17","author":"Pan","year":"2003","journal-title":"Genes Dev."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"322","DOI":"10.1016\/j.fgb.2012.01.010","article-title":"Pga13 in Candida albicans is localized in the cell wall and influences cell surface properties, morphogenesis and virulence","volume":"49","author":"Gelis","year":"2012","journal-title":"Fungal Genet. Biol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/13693780701435317","article-title":"Discovering the secrets of the Candida albicans agglutinin-like sequence (ALS) gene family\u2014A sticky pursuit","volume":"46","author":"Hoyer","year":"2008","journal-title":"Med. Mycol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1111\/j.1567-1364.2011.00727.x","article-title":"Pga26 mediates filamentation and biofilm formation and is required for virulence in Candida albicans","volume":"11","author":"Laforet","year":"2011","journal-title":"FEMS Yeast Res."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1111\/j.1567-1364.2005.00017.x","article-title":"Molecular organization of the cell wall of Candida albicans and its relation to pathogenicity","volume":"6","author":"Elorza","year":"2006","journal-title":"FEMS Yeast Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"4157","DOI":"10.1099\/mic.0.26924-0","article-title":"PST1 and ECM33 encode two yeast cell surface GPI proteins important for cell wall integrity","volume":"150","author":"Pardo","year":"2004","journal-title":"Microbiology"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"229","DOI":"10.1023\/A:1026007923908","article-title":"Cell wall composition and structure of Yarrowia lipolytica transposon mutants affected in calcofluor sensitivity","volume":"84","author":"Valentin","year":"2003","journal-title":"Antonie Van Leeuwenhoek"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"3671","DOI":"10.1073\/pnas.89.9.3671","article-title":"A heat shock gene from Saccharomyces cerevisiae encoding a secretory glycoprotein","volume":"89","author":"Russo","year":"1992","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"781","DOI":"10.1002\/yea.1007","article-title":"Genome-wide identification of fungal GPI proteins","volume":"20","author":"Hellingwerf","year":"2003","journal-title":"Yeast"},{"key":"ref_19","unstructured":"Mora-Montes, H.M. (2013). A Genomic Inventory of Cell Wall Biosynthesis in the Ubiquitous Plant Pathogen Botrytis cinerea. The Fungal Cell Wall, Nova Biomedical."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1002\/yea.1016","article-title":"Functional analysis of the cysteine residues and the repetitive sequence of Saccharomyces cerevisiae Pir4\/Cis3: The repetitive sequence is needed for binding to the cell wall beta-1,3-glucan","volume":"20","author":"Castillo","year":"2003","journal-title":"Yeast"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"11523","DOI":"10.1074\/jbc.M600314200","article-title":"Pir proteins of Saccharomyces cerevisiae are attached to beta-1,3-glucan by a new protein-carbohydrate linkage","volume":"281","author":"Ecker","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"3397","DOI":"10.1105\/tpc.109.065839","article-title":"The O-Mannosyltransferase PMT4 Is Essential for Normal Appressorium Formation and Penetration in Ustilago maydis","volume":"21","author":"Ibeas","year":"2009","journal-title":"Plant Cell"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1475","DOI":"10.1128\/EC.00040-09","article-title":"Aspergillus nidulans Protein O-Mannosyltransferases Play Roles in Cell Wall Integrity and Developmental Patterning","volume":"8","author":"Kriangkripipat","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"2260","DOI":"10.1128\/EC.00261-07","article-title":"O-mannosyltransferase 1 in Aspergillus fumigatus (AfPmt1p) is crucial for cell wall integrity and conidium morphology, especially at an elevated temperature","volume":"6","author":"Zhou","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1205","DOI":"10.1111\/j.1365-2958.2010.07164.x","article-title":"Members of protein O-mannosyltransferase family in Aspergillus fumigatus differentially affect growth, morphogenesis and viability","volume":"76","author":"Mouyna","year":"2010","journal-title":"Mol. Microbiol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1111\/j.1365-2958.2005.04692.x","article-title":"Protein O-mannosylation is crucial for cell wall integrity, septation and viability in fission yeast","volume":"57","author":"Willer","year":"2005","journal-title":"Mol. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"546","DOI":"10.1111\/j.1365-2958.2004.04401.x","article-title":"PMT family of Candida albicans: Five protein mannosyltransferase isoforms affect growth, morphogenesis and antifungal resistance","volume":"55","author":"Prill","year":"2005","journal-title":"Mol. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5752","DOI":"10.1002\/j.1460-2075.1996.tb00961.x","article-title":"The PMT gene family: Protein O-glycosylation in Saccharomyces cerevisiae is vital","volume":"15","author":"Gentzsch","year":"1996","journal-title":"Embo J."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"3871","DOI":"10.1002\/pmic.200800110","article-title":"A study of the Candida albicans cell wall proteome","volume":"8","author":"Castillo","year":"2008","journal-title":"Proteomics"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"124","DOI":"10.1016\/j.fgb.2005.12.002","article-title":"Genomic response programs of Candida albicans following protoplasting and regeneration","volume":"43","author":"Castillo","year":"2006","journal-title":"Fungal Genet. Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1128\/EC.00182-06","article-title":"Role of protein O-mannosyltransferase Pmt4 in the morphogenesis and virulence of Cryptococcus neoformans","volume":"6","author":"Olson","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"4571","DOI":"10.1128\/IAI.73.8.4571-4580.2005","article-title":"Virulence of the fungal pathogen Candida albicans requires the five isoforms of protein mannosyltransferases","volume":"73","author":"Rouabhia","year":"2005","journal-title":"Infect. Immun."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Willger, S.D., Ernst, J.F., Alspaugh, J.A., and Lengeler, K.B. (2009). Characterization of the PMT gene family in Cryptococcus neoformans. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0006321"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"542","DOI":"10.1093\/glycob\/cwp206","article-title":"Reduced expression of the O-mannosyltransferase 2 (AfPmt2) leads to deficient cell wall and abnormal polarity in Aspergillus fumigatus","volume":"20","author":"Fang","year":"2010","journal-title":"Glycobiology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"251","DOI":"10.18388\/abp.2008_3072","article-title":"Disruption of Trichoderma reesei gene encoding protein O-mannosyltransferase I results in a decrease of the enzyme activity and alteration of cell wall composition","volume":"55","author":"Pniewski","year":"2008","journal-title":"Acta Biochim. Pol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1973","DOI":"10.1099\/mic.0.27005-0","article-title":"Molecular characterization of protein O-mannosyltransferase and its involvement in cell-wall synthesis in Aspergillus nidulans","volume":"150","author":"Oka","year":"2004","journal-title":"Microbiology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"3657","DOI":"10.1099\/mic.0.28088-0","article-title":"Protein O-mannosyltransferase A of Aspergillus awamori is involved in O-mannosylation of glucoamylase I","volume":"151","author":"Oka","year":"2005","journal-title":"Microbiology"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Gonzalez, M., Brito, N., Frias, M., and Gonzalez, C. (2013). Botrytis cinerea Protein O-Mannosyltransferases Play Critical Roles in Morphogenesis, Growth, and Virulence. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0065924"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"445","DOI":"10.1007\/BF00351784","article-title":"Variations in Ploidy among Isolates of Botrytis cinerea\u2014Implications for Genetic and Molecular Analyses","volume":"25","author":"Buttner","year":"1994","journal-title":"Curr. Genet."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1006\/pmpp.1998.0142","article-title":"Detoxification of alpha-tomatine by Botrytis cinerea","volume":"52","author":"Quidde","year":"1998","journal-title":"Physiol. Mol. Plant Pathol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"2821","DOI":"10.1021\/jf030672j","article-title":"Characterization of the antifungal activity on Botrytis cinerea of the natural diterpenoids kaurenoic acid and 3beta-hydroxy-kaurenoic acid","volume":"52","author":"Cotoras","year":"2004","journal-title":"J. Agric. Food Chem."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"36","DOI":"10.1016\/j.fgb.2015.01.012","article-title":"bcpmr1 encodes a P-type Ca2+\/Mn2+-ATPase mediating cell-wall integrity and virulence in the phytopathogen Botrytis cinerea","volume":"76","author":"Plaza","year":"2015","journal-title":"Fungal Genet. Biol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"641","DOI":"10.1111\/j.1364-3703.2005.00312.x","article-title":"Functional analysis of Botrytis cinerea pectin methylesterase genes by PCR-based targeted mutagenesis: Bcpme1 and Bcpme2 are dispensable for virulence of strain B05.10","volume":"6","author":"Kars","year":"2005","journal-title":"Mol. Plant Pathol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"230","DOI":"10.1007\/s10327-011-0311-4","article-title":"Bchex virulence gene of Botrytis cinerea: Characterization and functional analysis","volume":"77","author":"Aguayo","year":"2011","journal-title":"J. Gen. Plant Pathol."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Meuer, S., Wittwer, C., and Nakagawara, K.-I. (2001). Quantification on the LightCycler. Rapid Cycle Real-Time PCR: Methods and Applications, Springer.","DOI":"10.1007\/978-3-642-59524-0"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"402","DOI":"10.1006\/meth.2001.1262","article-title":"Analysis of relative gene expression data using real-time quantitative PCR and the 2\u2212\u0394\u0394CT method","volume":"25","author":"Livak","year":"2001","journal-title":"Methods"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"990","DOI":"10.1016\/j.fgb.2011.06.002","article-title":"The D-galacturonic acid catabolic pathway in Botrytis cinerea","volume":"48","author":"Zhang","year":"2011","journal-title":"Fungal Genet. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1038\/nprot.2007.13","article-title":"High-efficiency yeast transformation using the LiAc\/SS carrier DNA\/PEG method","volume":"2","author":"Gietz","year":"2007","journal-title":"Nat. Protoc."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"2184","DOI":"10.1128\/EC.00350-07","article-title":"Endoplasmic reticulum alpha-glycosidases of Candida albicans are required for N glycosylation, cell wall integrity, and normal host-fungus interaction","volume":"6","author":"Bates","year":"2007","journal-title":"Eukaryot. Cell"},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"3104","DOI":"10.1128\/jb.177.11.3104-3110.1995","article-title":"Identification of three mannoproteins in the cell wall of Saccharomyces cerevisiae","volume":"177","author":"Caro","year":"1995","journal-title":"J. Bacteriol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1480","DOI":"10.1091\/mbc.e06-11-1049","article-title":"A phenomics approach in yeast links proton and calcium pump function in the Golgi","volume":"18","author":"Yadav","year":"2007","journal-title":"Mol. Biol. Cell"},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1111\/j.1365-2958.2005.04991.x","article-title":"Different signalling pathways involving a Galpha protein, cAMP and a MAP kinase control germination of Botrytis cinerea conidia","volume":"59","author":"Doehlemann","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.ijfoodmicro.2018.08.027","article-title":"Antifungal activity of proteolytic fraction (P1G10) from (Vasconcellea cundinamarcensis) latex inhibit cell growth and cell wall integrity in Botrytis cinerea","volume":"289","author":"Salas","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"ref_54","doi-asserted-by":"crossref","unstructured":"Torres-Ossandon, M.J., Castillo, L., Uribe, E., Bilbao-Sainz, C., Ah-Hen, K.S., and Vega-Galvez, A. (2023). Combined Effect of High Hydrostatic Pressure and Proteolytic Fraction P1G10 from Vasconcellea cundinamarcensis Latex against Botrytis cinerea in Grape Juice. Foods, 12.","DOI":"10.3390\/foods12183400"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1016\/j.ifset.2015.01.010","article-title":"Effect of high hydrostatic pressure (HHP) treatments on microbiological shelf-life of chilled Chilean jack mackerel (Trachurus murphyi)","volume":"29","author":"Reyes","year":"2015","journal-title":"Innov. Food Sci. Emerg. Technol."},{"key":"ref_56","first-page":"3916","article-title":"Purification and visualization of lipopolysaccharide from Gram-negative bacteria by hot aqueous-phenol extraction","volume":"63","author":"Davis","year":"2012","journal-title":"J. Vis. Exp."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"9068","DOI":"10.1074\/jbc.274.13.9068","article-title":"Transmembrane topology of pmt1p, a member of an evolutionarily conserved family of protein O-mannosyltransferases","volume":"274","author":"Scheinost","year":"1999","journal-title":"J. Biol. Chem."},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"567","DOI":"10.1006\/jmbi.2000.4315","article-title":"Predicting transmembrane protein topology with a hidden Markov model: Application to complete genomes","volume":"305","author":"Krogh","year":"2001","journal-title":"J. Mol. Biol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"90","DOI":"10.1074\/jbc.M510360200","article-title":"Outer chain N-glycans are required for cell wall integrity and virulence of Candida albicans","volume":"281","author":"Bates","year":"2006","journal-title":"J. Biol. Chem."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"44","DOI":"10.1111\/j.1469-0691.2008.02676.x","article-title":"Genomics in the detection of damage in microbial systems: Cell wall stress in yeast","volume":"15","author":"Arroyo","year":"2009","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"1533","DOI":"10.1091\/mbc.e03-07-0511","article-title":"O-glycosylation as a sorting determinant for cell surface delivery in yeast","volume":"15","author":"Proszynski","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"1019","DOI":"10.1002\/yea.320100804","article-title":"A new approach for isolating cell wall mutants in Saccharomyces cerevisiae by screening for hypersensitivity to calcofluor white","volume":"10","author":"Ram","year":"1994","journal-title":"Yeast"},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"127","DOI":"10.1111\/j.1749-6632.2009.05182.x","article-title":"High hydrostatic pressure and the cell membrane: Stress response of Saccharomyces cerevisiae","volume":"1189","author":"Bravim","year":"2010","journal-title":"Ann. N. Y. Acad. Sci."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"21310","DOI":"10.3390\/molecules191221310","article-title":"Effects of high hydrostatic pressure on Escherichia coli ultrastructure, membrane integrity and molecular composition as assessed by FTIR spectroscopy and microscopic imaging techniques","volume":"19","author":"Prieto","year":"2014","journal-title":"Molecules"},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3488","DOI":"10.1128\/AAC.00606-06","article-title":"Protein O-mannosyltransferase isoforms regulate biofilm formation in Candida albicans","volume":"50","author":"Goyard","year":"2006","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_66","doi-asserted-by":"crossref","unstructured":"Bates, S., Hall, R.A., Cheetham, J., Netea, M.G., MacCallum, D.M., Brown, A.J., Odds, F.C., and Gow, N.A. (2013). Role of the Candida albicans MNN1 gene family in cell wall structure and virulence. BMC Res. Notes, 6.","DOI":"10.1186\/1756-0500-6-294"},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"472","DOI":"10.1128\/EC.00378-09","article-title":"The Aspergillus fumigatus P-Type Golgi Apparatus Ca2+\/Mn2+ ATPase PmrA Is Involved in Cation Homeostasis and Cell Wall Integrity but Is Not Essential for Pathogenesis","volume":"9","author":"Pinchai","year":"2010","journal-title":"Eukaryot. Cell"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"23408","DOI":"10.1074\/jbc.M502162200","article-title":"Candida albicans Pmr1p, a secretory pathway P-type Ca2+\/Mn2+-ATPase, is required for glycosylation and virulence","volume":"280","author":"Bates","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_69","doi-asserted-by":"crossref","first-page":"9335","DOI":"10.1074\/jbc.M114.616334","article-title":"Manganese redistribution by calcium-stimulated vesicle trafficking bypasses the need for P-type ATPase function","volume":"290","author":"Muniz","year":"2015","journal-title":"J. Biol. Chem."},{"key":"ref_70","doi-asserted-by":"crossref","first-page":"1124","DOI":"10.1128\/EC.3.5.1124-1135.2004","article-title":"Schizosaccharomyces pombe Pmr1p is essential for cell wall integrity and is required for polarized cell growth and cytokinesis","volume":"3","author":"Cortes","year":"2004","journal-title":"Eukaryot. Cell"},{"key":"ref_71","doi-asserted-by":"crossref","first-page":"553","DOI":"10.1111\/j.1365-2958.2009.06786.x","article-title":"Dynamics of cell wall components of Magnaporthe grisea during infectious structure development","volume":"73","author":"Fujikawa","year":"2009","journal-title":"Mol. Microbiol."},{"key":"ref_72","doi-asserted-by":"crossref","unstructured":"Navarro-Arias, M.J., Defosse, T.A., Dementhon, K., Csonka, K., Mellado-Mojica, E., Dias Valerio, A., Gonzalez-Hernandez, R.J., Courdavault, V., Clastre, M., and Hernandez, N.V. (2016). Disruption of Protein Mannosylation Affects Candida guilliermondii Cell Wall, Immune Sensing, and Virulence. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.01951"},{"key":"ref_73","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1128\/jb.179.2.463-469.1997","article-title":"Increase in chitin as an essential response to defects in assembly of cell wall polymers in the ggp1delta mutant of Saccharomyces cerevisiae","volume":"179","author":"Popolo","year":"1997","journal-title":"J. Bacteriol."},{"key":"ref_74","doi-asserted-by":"crossref","first-page":"6279","DOI":"10.1128\/jb.179.20.6279-6284.1997","article-title":"Altered extent of cross-linking of beta1,6-glucosylated mannoproteins to chitin in Saccharomyces cerevisiae mutants with reduced cell wall beta1,3-glucan content","volume":"179","author":"Kapteyn","year":"1997","journal-title":"J. Bacteriol."},{"key":"ref_75","doi-asserted-by":"crossref","first-page":"1167","DOI":"10.2217\/fmb.09.88","article-title":"Protein glycosylation in Candida","volume":"4","author":"Gow","year":"2009","journal-title":"Future Microbiol."},{"key":"ref_76","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1002\/yea.678","article-title":"Mutation of the homologue of GDP-mannose pyrophosphorylase alters cell wall structure, protein glycosylation and secretion in Hansenula polymorpha","volume":"18","author":"Agaphonov","year":"2001","journal-title":"Yeast"},{"key":"ref_77","doi-asserted-by":"crossref","first-page":"1833","DOI":"10.1083\/jcb.110.5.1833","article-title":"Yeast KRE genes provide evidence for a pathway of cell wall beta-glucan assembly","volume":"110","author":"Boone","year":"1990","journal-title":"J. Cell Biol."},{"key":"ref_78","first-page":"6346","article-title":"The yeast KRE9 gene encodes an O glycoprotein involved in cell surface beta-glucan assembly","volume":"13","author":"Brown","year":"1993","journal-title":"Mol. Cell. Biol."},{"key":"ref_79","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1002\/yea.320060606","article-title":"The glucanase-soluble mannoproteins limit cell wall porosity in Saccharomyces cerevisiae","volume":"6","author":"Klis","year":"1990","journal-title":"Yeast"},{"key":"ref_80","doi-asserted-by":"crossref","unstructured":"Lipke, P.N. (2018). What We Do Not Know about Fungal Cell Adhesion Molecules. J. Fungi, 4.","DOI":"10.3390\/jof4020059"},{"key":"ref_81","doi-asserted-by":"crossref","first-page":"1051","DOI":"10.1074\/jbc.M411413200","article-title":"Mnt1p and Mnt2p of Candida albicans are partially redundant alpha-1,2-mannosyltransferases that participate in O-linked mannosylation and are required for adhesion and virulence","volume":"280","author":"Munro","year":"2005","journal-title":"J. Biol. Chem."},{"key":"ref_82","doi-asserted-by":"crossref","first-page":"1786","DOI":"10.1128\/aem.59.6.1786-1791.1993","article-title":"Adhesion of Nongerminated Botrytis cinerea Conidia to Several Substrata","volume":"59","author":"Doss","year":"1993","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_83","doi-asserted-by":"crossref","first-page":"260","DOI":"10.1128\/aem.61.1.260-265.1995","article-title":"Adhesion of germlings of Botrytis cinerea","volume":"61","author":"Doss","year":"1995","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_84","doi-asserted-by":"crossref","first-page":"470","DOI":"10.1128\/EC.00364-12","article-title":"Adhesins in human fungal pathogens: Glue with plenty of stick","volume":"12","author":"Bader","year":"2013","journal-title":"Eukaryot. Cell"},{"key":"ref_85","doi-asserted-by":"crossref","first-page":"282","DOI":"10.1128\/MMBR.00037-06","article-title":"A biochemical guide to yeast adhesins: Glycoproteins for social and antisocial occasions","volume":"71","author":"Dranginis","year":"2007","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_86","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/j.bbamem.2004.08.002","article-title":"Membrane fluidity and its roles in the perception of environmental signals","volume":"1666","author":"Los","year":"2004","journal-title":"Biochim. Biophys. Acta"},{"key":"ref_87","doi-asserted-by":"crossref","unstructured":"Marin-Menguiano, M., Moreno-Sanchez, I., Barrales, R.R., Fernandez-Alvarez, A., and Ibeas, J.I. (2019). N-glycosylation of the protein disulfide isomerase Pdi1 ensures full Ustilago maydis virulence. PLoS Pathog., 15.","DOI":"10.1101\/571125"},{"key":"ref_88","doi-asserted-by":"crossref","first-page":"1203","DOI":"10.1590\/S0100-879X2005000800007","article-title":"The effect of hydrostatic pressure on membrane-bound proteins","volume":"38","author":"Scarlata","year":"2005","journal-title":"Braz. J. Med. Biol. Res."},{"key":"ref_89","doi-asserted-by":"crossref","unstructured":"Sun, J.Z., Pang, C.Y., Cheng, X., Yang, B.Y., Jin, B.B., Jin, L., Qi, Y.X., Sun, Y., Chen, X., and Liu, W.D. (2023). Investigation of the antifungal activity of the dicarboximide fungicide iprodione against Bipolaris maydis. Pestic. Biochem. Phys., 190.","DOI":"10.1016\/j.pestbp.2022.105319"},{"key":"ref_90","doi-asserted-by":"crossref","first-page":"3933","DOI":"10.1128\/AAC.00615-08","article-title":"Genetic analysis of fenhexamid-resistant field isolates of the phytopathogenic fungus Botrytis cinerea","volume":"52","author":"Fillinger","year":"2008","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_91","doi-asserted-by":"crossref","unstructured":"Chen, X.L., Liu, C., Tang, B., Ren, Z., Wang, G.L., and Liu, W. (2020). Quantitative proteomics analysis reveals important roles of N-glycosylation on ER quality control system for development and pathogenesis in Magnaporthe oryzae. PLoS Pathog., 16.","DOI":"10.1371\/journal.ppat.1008355"},{"key":"ref_92","doi-asserted-by":"crossref","unstructured":"Perez-Garcia, L.A., Csonka, K., Flores-Carreon, A., Estrada-Mata, E., Mellado-Mojica, E., Nemeth, T., Lopez-Ramirez, L.A., Toth, R., Lopez, M.G., and Vizler, C. (2016). Role of Protein Glycosylation in Candida parapsilosis Cell Wall Integrity and Host Interaction. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00306"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/11\/1\/71\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,8]],"date-time":"2025-10-08T10:30:19Z","timestamp":1759919419000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/11\/1\/71"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,1,17]]},"references-count":92,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2025,1]]}},"alternative-id":["jof11010071"],"URL":"https:\/\/doi.org\/10.3390\/jof11010071","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,1,17]]}}}