{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T22:46:25Z","timestamp":1765233985817,"version":"build-2065373602"},"reference-count":33,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T00:00:00Z","timestamp":1743465600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Project of Shandong Province Higher Educational Youth Innovation Science and Technology Program","award":["2022KJ096","XKY2410"],"award-info":[{"award-number":["2022KJ096","XKY2410"]}]},{"name":"Science and Technology Program of University of Jinan","award":["2022KJ096","XKY2410"],"award-info":[{"award-number":["2022KJ096","XKY2410"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The Qinghai-Tibet Plateau, known as \u201cthe third pole\u201d of the Earth, boasts unique climatic conditions with abundant sunlight, low temperature, and significant annual temperature variations, nurturing a diverse array of soil microorganisms with rich metabolic products. In this study, 132 fungal isolates were isolated and identified from the soil samples collected in Nyingchi, Tibet, belonging to 32 genera and 59 species, while Ascomycota, Mucoromycota, and Basidiomycota accounted for 91.7%, 7.5%, and 0.8%, respectively. The evolutionary relationships among 59 representative strains were revealed by constructing a phylogenetic tree, while Penicillium, Fusarium, and Aspergillus were the most widespread fungal genera here, and the antibacterial activity of these strains was evaluated by the agar diffusion assay, leading to 27 active strains. Notably, six of them demonstrated significant activities against two or more tested bacteria. The antibacterial efficacy of the extracts of these six fungi, which were derived from four distinct media, was further evaluated at a concentration of 500 \u03bcg\/mL. This study provides a valuable supplement to the investigations of cultivable soil fungi in Nyingchi, Tibet, laying a foundation for the development of soil fungi and antibacterial lead compounds in the area.<\/jats:p>","DOI":"10.3390\/jof11040276","type":"journal-article","created":{"date-parts":[[2025,4,1]],"date-time":"2025-04-01T11:08:40Z","timestamp":1743505720000},"page":"276","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Exploring the Biodiversity and Antibacterial Potential of the Culturable Soil Fungi in Nyingchi, Tibet"],"prefix":"10.3390","volume":"11","author":[{"given":"Shan-Shan","family":"Huang","sequence":"first","affiliation":[{"name":"School of Biological Science and Technology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Haishan","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-1040-1611","authenticated-orcid":false,"given":"Xia-Fei","family":"Li","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun-Ying","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiujun","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan-Juan","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9162-3355","authenticated-orcid":false,"given":"Fuhang","family":"Song","sequence":"additional","affiliation":[{"name":"Key Laboratory of Geriatric Nutrition and Health, Ministry of Education of China, School of Light Industry, Beijing Technology and Business University, Beijing 100048, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jie","family":"Bao","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5959-6473","authenticated-orcid":false,"given":"Hua","family":"Zhang","sequence":"additional","affiliation":[{"name":"School of Biological Science and Technology, University of Jinan, 336 West Road of Nan Xinzhuang, Jinan 250022, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,4,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"371","DOI":"10.1016\/j.ijantimicag.2018.11.010","article-title":"Antibiotic discovery: History, methods and perspectives","volume":"53","author":"Durand","year":"2019","journal-title":"Int. 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