{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,19]],"date-time":"2026-04-19T06:59:07Z","timestamp":1776581947140,"version":"3.51.2"},"reference-count":90,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T00:00:00Z","timestamp":1746144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n","award":["Ciencia de Frontera 2019-6380"],"award-info":[{"award-number":["Ciencia de Frontera 2019-6380"]}]},{"name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n","award":["CBF2023-2024-655"],"award-info":[{"award-number":["CBF2023-2024-655"]}]},{"name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n","award":["Ciencia de Frontera 2019-6380"],"award-info":[{"award-number":["Ciencia de Frontera 2019-6380"]}]},{"name":"Secretar\u00eda de Ciencia, Humanidades, Tecnolog\u00eda e Innovaci\u00f3n","award":["CBF2023-2024-655"],"award-info":[{"award-number":["CBF2023-2024-655"]}]},{"name":"Red Tem\u00e1tica Glicociencia en Salud [CONACYT-M\u00e9xico]","award":["Ciencia de Frontera 2019-6380"],"award-info":[{"award-number":["Ciencia de Frontera 2019-6380"]}]},{"name":"Red Tem\u00e1tica Glicociencia en Salud [CONACYT-M\u00e9xico]","award":["CBF2023-2024-655"],"award-info":[{"award-number":["CBF2023-2024-655"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Sporothrix schenckii is a pathogenic fungus of worldwide distribution and one of the etiological agents of sporotrichosis. The cell wall is the first point of contact with host cells; therefore, its composition has been widely studied. It has a cell wall composed of chitin, \u03b2-glucans, and glycoproteins modified with N-linked and O-linked glycans. Protein O-linked glycosylation is mediated by two gene families, PMT and MNT. Therefore, we evaluated the relevance of protein O-linked glycosylation during the interaction of S. schenckii with the host. Independent silencing of the MNT1 and PMT2 was accomplished by interference RNA. Morphological analyses revealed defects in cell morphology in both yeast and mycelial cells; however, these defects differed between MNT1 and PMT2 silencing. Subsequently, the cell wall was characterized, and the silencing of these genes markedly changed cell wall organization. When the silenced strains interacted with human peripheral blood mononuclear cells, a reduced ability to stimulate the proinflammatory cytokines IL-6 and TNF\u03b1 was found. However, the PMT2-silenced mutants also stimulated higher levels of IL-10 and IL-1\u03b2. Interaction with macrophages and neutrophils was also altered, with increased phagocytosis and decreased extracellular trap formation in both sets of silenced strains. Survival assays in Galleria mellonella larvae showed that silencing of any of these genes reduced the ability of S. schenckii to kill the host. In addition, the mutant strains showed defects in the adhesion to extracellular matrix proteins. These data indicate that MNT1 and PMT2 are relevant for cell wall synthesis and interaction with the host.<\/jats:p>","DOI":"10.3390\/jof11050352","type":"journal-article","created":{"date-parts":[[2025,5,2]],"date-time":"2025-05-02T07:44:23Z","timestamp":1746171863000},"page":"352","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Silencing of MNT1 and PMT2 Shows the Importance of O-Linked Glycosylation During the Sporothrix schenckii\u2013Host Interaction"],"prefix":"10.3390","volume":"11","author":[{"given":"Manuela","family":"G\u00f3mez-Gaviria","sequence":"first","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s\/n, col. Noria Alta, C.P., Guanajuato 36050, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4987-086X","authenticated-orcid":false,"given":"Jos\u00e9 A.","family":"Mart\u00ednez-\u00c1lvarez","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s\/n, col. Noria Alta, C.P., Guanajuato 36050, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Iv\u00e1n","family":"Mart\u00ednez-Duncker","sequence":"additional","affiliation":[{"name":"Laboratorio de Glicobiolog\u00eda Humana y Diagn\u00f3stico Molecular, Centro de Investigaci\u00f3n en Din\u00e1mica Celular, Instituto de Investigaci\u00f3n en Ciencias B\u00e1sicas y Aplicadas, Universidad Aut\u00f3noma del Estado de Morelos, Cuernavaca 62209, Mexico"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0461-7521","authenticated-orcid":false,"given":"Andrea Regina de Souza","family":"Baptista","sequence":"additional","affiliation":[{"name":"Center for Microorganism\u2019 Research, Biomedical Institute, Fluminense Federal University, Campus Valonguinho-Alameda Barros Terra, S\/N, Niter\u00f3i 24020-150, RJ, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6973-0595","authenticated-orcid":false,"given":"H\u00e9ctor M.","family":"Mora-Montes","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda, Divisi\u00f3n de Ciencias Naturales y Exactas, Campus Guanajuato, Universidad de Guanajuato, Noria Alta s\/n, col. 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