{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T01:10:49Z","timestamp":1792113049246,"version":"4.4.0"},"reference-count":48,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2025,5,17]],"date-time":"2025-05-17T00:00:00Z","timestamp":1747440000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Open Research Fund Program of Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-construction by Ministry and Province)","award":["AHYY2023006"],"award-info":[{"award-number":["AHYY2023006"]}]},{"name":"Open Research Fund Program of Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-construction by Ministry and Province)","award":["XXBS-202301"],"award-info":[{"award-number":["XXBS-202301"]}]},{"name":"Open Research Fund Program of Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-construction by Ministry and Province)","award":["2024YL035"],"award-info":[{"award-number":["2024YL035"]}]},{"name":"Open Research Fund Program of Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization (Co-construction by Ministry and Province)","award":["2021711"],"award-info":[{"award-number":["2021711"]}]},{"name":"Talent Introduction Program of Anhui Academy of Agricultural Sciences","award":["AHYY2023006"],"award-info":[{"award-number":["AHYY2023006"]}]},{"name":"Talent Introduction Program of Anhui Academy of Agricultural Sciences","award":["XXBS-202301"],"award-info":[{"award-number":["XXBS-202301"]}]},{"name":"Talent Introduction Program of Anhui Academy of Agricultural Sciences","award":["2024YL035"],"award-info":[{"award-number":["2024YL035"]}]},{"name":"Talent Introduction Program of Anhui Academy of Agricultural Sciences","award":["2021711"],"award-info":[{"award-number":["2021711"]}]},{"name":"Quality Vegetable New Breeds and Green &amp; Efficient Cultivation Technology Transformation and Application","award":["AHYY2023006"],"award-info":[{"award-number":["AHYY2023006"]}]},{"name":"Quality Vegetable New Breeds and Green &amp; Efficient Cultivation Technology Transformation and Application","award":["XXBS-202301"],"award-info":[{"award-number":["XXBS-202301"]}]},{"name":"Quality Vegetable New Breeds and Green &amp; Efficient Cultivation Technology Transformation and Application","award":["2024YL035"],"award-info":[{"award-number":["2024YL035"]}]},{"name":"Quality Vegetable New Breeds and Green &amp; Efficient Cultivation Technology Transformation and Application","award":["2021711"],"award-info":[{"award-number":["2021711"]}]},{"name":"Anhui Vegetable Industry Technology System Fund","award":["AHYY2023006"],"award-info":[{"award-number":["AHYY2023006"]}]},{"name":"Anhui Vegetable Industry Technology System Fund","award":["XXBS-202301"],"award-info":[{"award-number":["XXBS-202301"]}]},{"name":"Anhui Vegetable Industry Technology System Fund","award":["2024YL035"],"award-info":[{"award-number":["2024YL035"]}]},{"name":"Anhui Vegetable Industry Technology System Fund","award":["2021711"],"award-info":[{"award-number":["2021711"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>With the increasing scarcity of traditional hardwood sawdust resources, developing sustainable substrates for edible fungi cultivation has become an urgent industrial priority. This study systematically evaluated the effects of bamboo sawdust substitutions (20%, 30%, 40%, and 50%) on mycelial growth, fruiting body development, and nutritional quality of Auricularia heimuer, while elucidating the underlying molecular mechanisms through transcriptome sequencing. The results demonstrated that bamboo substitution of \u226430% maintained normal mycelial growth and fruiting body differentiation, with 20% and 30% substitutions increasing yields by 5.30% and 3.70%, respectively, compared to the control. However, 50% substitution significantly reduced yield by 9.49%. Nutritional analysis revealed that 20\u201340% bamboo substitution significantly enhanced the contents of crude protein, polysaccharides, and essential minerals (calcium, iron, and selenium) in fruiting bodies. Transcriptome analysis identified upregulation of glycosyl hydrolase family genes and downregulation of redox-related genes with increasing bamboo proportions. Biochemical assays confirmed these findings, showing decreased oxidative substances and increased reductive compounds in mycelia grown with high bamboo content, which indicate disrupted cellular redox homeostasis. This study provides both a practical solution to alleviate the \u201cedible mushrooms derived from lignicolous fungi\u2013forest conflict\u201d and fundamental insights into fungal adaptation mechanisms to non-wood substrates, thus establishing a theoretical foundation for the valorization of agricultural and forestry wastes.<\/jats:p>","DOI":"10.3390\/jof11050387","type":"journal-article","created":{"date-parts":[[2025,5,19]],"date-time":"2025-05-19T06:31:29Z","timestamp":1747636289000},"page":"387","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Feasibility of Bamboo Sawdust as Sustainable Alternative Substrate for Auricularia heimuer Cultivation"],"prefix":"10.3390","volume":"11","author":[{"given":"Ya-Hui","family":"Wang","sequence":"first","affiliation":[{"name":"Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization, Co-Construction by Ministry and Province, Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230001, China"},{"name":"Institute of Vegetables, Anhui Academy of Agricultural Sciences, Hefei 230001, China"},{"name":"Edible and Medicinal Mushroom Innovation Centre, Anhui Academy of Agricultural Sciences, Hefei 230001, China"},{"name":"Dongzhi Black Fungus Industrial Technology Research and Development Centre, Chizhou 247200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cong-Sheng","family":"Yan","sequence":"additional","affiliation":[{"name":"Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization, Co-Construction by Ministry and Province, Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230001, China"},{"name":"Institute of Vegetables, Anhui Academy of Agricultural Sciences, Hefei 230001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yong-Jin","family":"Deng","sequence":"additional","affiliation":[{"name":"Sericulture Research Institute, Anhui Academy of Agricultural Sciences, Hefei 230001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zheng-Fu","family":"Zhu","sequence":"additional","affiliation":[{"name":"Institute of Vegetables, Anhui Academy of Agricultural Sciences, Hefei 230001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hua-An","family":"Sun","sequence":"additional","affiliation":[{"name":"Institute of Vegetables, Anhui Academy of Agricultural Sciences, Hefei 230001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4652-5278","authenticated-orcid":false,"given":"Hui-Ping","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hong-Yuan","family":"Zhao","sequence":"additional","affiliation":[{"name":"Institute of Vegetables, Anhui Academy of Agricultural Sciences, Hefei 230001, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guo-Qing","family":"Li","sequence":"additional","affiliation":[{"name":"Key Laboratory of Horticultural Crop Germplasm Innovation and Utilization, Co-Construction by Ministry and Province, Institute of Horticultural, Anhui Academy of Agricultural Sciences, Hefei 230001, China"},{"name":"Institute of Vegetables, Anhui Academy of Agricultural Sciences, Hefei 230001, China"},{"name":"Edible and Medicinal Mushroom Innovation Centre, Anhui Academy of Agricultural Sciences, Hefei 230001, China"},{"name":"Dongzhi Black Fungus Industrial Technology Research and Development Centre, Chizhou 247200, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,5,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"3949","DOI":"10.1007\/s00253-022-11985-0","article-title":"Edible mushroom industry in China: Current state and perspectives","volume":"106","author":"Li","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1355","DOI":"10.1007\/s00253-021-11749-2","article-title":"Challenges and opportunities in producing high-quality edible mushrooms from lignocellulosic biomass in a small scale","volume":"106","author":"Balan","year":"2022","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1128\/MMBR.00035-14","article-title":"Plant-polysaccharide-degrading enzymes from basidiomycetes","volume":"78","author":"Rytioja","year":"2014","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Xu, L., Yang, W., Qiu, T., Gao, X., Zhang, H., Zhang, S., Cui, H., Guo, L., Yu, H., and Yu, H. (2023). Complete genome sequences and comparative secretomic analysis for the industrially cultivated edible mushroom Lyophyllum decastes reveals insights on evolution and lignocellulose degradation potential. Front. Microbiol., 14.","DOI":"10.3389\/fmicb.2023.1137162"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1615\/IntJMedMushrooms.v19.i4.80","article-title":"Effect of different proportions of agrowaste on cultivation yield and nutritional composition of the culinary-medicinal jelly mushroom Auricularia polytricha (Higher Basidiomycetes)","volume":"19","author":"Wu","year":"2017","journal-title":"Int. J. Med. Mushrooms"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"2961","DOI":"10.3390\/su6052961","article-title":"Edible mushroom cultivation for food security and rural development in China: Bio-innovation, technological dissemination and marketing","volume":"6","author":"Zhang","year":"2014","journal-title":"Sustainability"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"111942","DOI":"10.1016\/j.scienta.2023.111942","article-title":"Comprehensive evaluation of agronomic traits and mineral elements of Auricularia heimuer cultivated on corncob substrates","volume":"314","author":"Xu","year":"2023","journal-title":"Sci. Hortic."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Fang, M., Sun, X., Yao, F., Lu, L., Ma, X., Shao, K., and Kaimoyo, E. (2024). A combination of transcriptome and enzyme activity analysis unveils key genes and patterns of corncob lignocellulose degradation by Auricularia heimuer under cultivation conditions. J. Fungi, 10.","DOI":"10.3390\/jof10080545"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"uhad122","DOI":"10.1093\/hr\/uhad122","article-title":"Single-cell transcriptome atlas reveals spatiotemporal developmental trajectories in the basal roots of moso bamboo (Phyllostachys edulis)","volume":"10","author":"Cheng","year":"2023","journal-title":"Hortic. Res."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"117210","DOI":"10.1016\/j.indcrop.2023.117210","article-title":"Bamboos as a cultivated medicinal grass for industries: A systematic review","volume":"203","author":"Sawarkar","year":"2023","journal-title":"Ind. Crops Prod."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1926","DOI":"10.15376\/biores.17.1.Dlamini","article-title":"Bamboo as a valuable resource and its utilization in historical and modern-day China","volume":"17","author":"Dlamini","year":"2022","journal-title":"Bioresources"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1007\/s12229-011-9082-z","article-title":"Review of carbon fixation in bamboo forests in China","volume":"77","author":"Zhou","year":"2011","journal-title":"Bot. Rev."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1007\/s13199-020-00673-0","article-title":"Bamboo nutrients and microbiome affect gut microbiome of giant panda","volume":"80","author":"Jin","year":"2020","journal-title":"Symbiosis"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s10570-022-04882-2","article-title":"Short-term thermal drying-induced pore expansion effects of cellulosic fibers and its applications","volume":"30","author":"Mo","year":"2023","journal-title":"Cellulose"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1980","DOI":"10.1038\/s41396-022-01247-2","article-title":"Adaptation of gut microbiome and host metabolic systems to lignocellulosic degradation in bamboo rats","volume":"16","author":"Xiao","year":"2022","journal-title":"ISME J."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1653","DOI":"10.1093\/treephys\/tpad074","article-title":"Inhibition of DNA and RNA methylation disturbs root development of moso bamboo","volume":"43","author":"Liufu","year":"2023","journal-title":"Tree Physiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"96","DOI":"10.1111\/1442-1984.12076","article-title":"Comparisons of soil-water content between a Moso bamboo (Phyllostachys pubescens) forest and an evergreen broadleaved forest in western Japan","volume":"30","author":"Shinohara","year":"2015","journal-title":"Plant Species Biol."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Chutimanukul, P., Phatthanamas, W., Thepsilvisut, O., Chantarachot, T., Thongtip, A., and Chutimanukul, P. (2023). Commercial scale production of Yamabushitake mushroom (Hericium erinaceus (Bull.) Pers. 1797) using rubber and bamboo sawdust substrates in tropical regions. Sci. Rep., 13.","DOI":"10.1038\/s41598-023-40601-y"},{"key":"ref_19","first-page":"1416","article-title":"Feasibility of Auricularia cornea cultivation with bamboo sawdust instead of wood sawdust and comprehensive evaluation of quality","volume":"35","author":"Ye","year":"2023","journal-title":"Acta Agric. Zhejiangensis"},{"key":"ref_20","first-page":"30","article-title":"Experiment of cultivating Lentinula edodes with Phyllostachys edulis sawdust","volume":"41","author":"Zeng","year":"2022","journal-title":"Edible Fungi China"},{"key":"ref_21","first-page":"36","article-title":"Study on Auricularia auricula-judae cultivation using waste of Phyllostachys edulis processing as substrate","volume":"34","author":"Fu","year":"2015","journal-title":"Edible Fungi China"},{"key":"ref_22","first-page":"89","article-title":"Auricularia heimuer new cultivar \u2018Wanheimuer 1(A3)\u2019","volume":"50","author":"Li","year":"2023","journal-title":"Acta Hortic. Sin."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"129651","DOI":"10.1016\/j.foodchem.2021.129651","article-title":"Nutritional value and biological properties of Chilean wild and commercial edible mushrooms","volume":"356","author":"Valderrama","year":"2021","journal-title":"Food Chem."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1556\/066.2016.45.1.3","article-title":"Chemical analysis of king oyster mushroom pleurotus eryngii fruitbodies","volume":"45","author":"Kruzselyi","year":"2016","journal-title":"Acta Aliment."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"64","DOI":"10.1080\/21501203.2014.917733","article-title":"Nutritional composition of two wild mushrooms consumed by the tribals of the Western Ghats of India","volume":"5","author":"Sudheep","year":"2014","journal-title":"Mycology"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"643","DOI":"10.1016\/j.foodchem.2005.09.087","article-title":"Non-volatile components of several novel species of edible fungi in China","volume":"100","author":"Guo","year":"2007","journal-title":"Food Chem."},{"key":"ref_27","first-page":"89","article-title":"Comparison of phenol-sulfuric acid method and anthrone sulfate method for determining the content of Ganoderma lucidum polysaccharides in Ganoderma lucidum spore","volume":"14","year":"2024","journal-title":"China Food Saf."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"490","DOI":"10.1016\/j.jksus.2018.04.021","article-title":"Cultivation of oyster mushroom (Pleurotus ostrreatus) on fermented moso bamboo sawdust","volume":"31","author":"Yamauchi","year":"2019","journal-title":"J. King Saud Univ. Sci."},{"key":"ref_29","unstructured":"Wu, Y., Ding, L., Li, S.-p., Zhang, Z., and Wu, Y.-l. (2012, January 23\u201324). The analysis of selenoprotein and Se-polysaccharide in selenium-enriched Lentinan edodes. Proceedings of the 1st International Conference on Energy and Environmental Protection (ICEEP 2012), Hohhot, China."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"e00648-18","DOI":"10.1128\/AAC.00648-18","article-title":"Biochemical and structural basis of triclosan resistance in a novel Enoyl-Acyl carrier protein reductase","volume":"62","author":"Khan","year":"2018","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"2213","DOI":"10.1016\/j.str.2015.09.013","article-title":"Structural basis for cyclopropanation by a unique Enoyl-Acyl carrier protein reductase","volume":"23","author":"Khare","year":"2015","journal-title":"Structure"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"de Ramon-Carbonell, M., and Sanchez-Torres, P. (2021). Penicillium digitatum MFS transporters can display different roles during pathogen-fruit interaction. Int. J. Food Microbiol., 337.","DOI":"10.1016\/j.ijfoodmicro.2020.108918"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.tibs.2013.01.003","article-title":"Structural advances for the major facilitator superfamily (MFS) transporters","volume":"38","author":"Yan","year":"2013","journal-title":"Trends Biochem. Sci."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"123","DOI":"10.1038\/nrm.2015.25","article-title":"Understanding transport by the major facilitator superfamily (MFS): Structures pave the way","volume":"17","author":"Quistgaard","year":"2016","journal-title":"Nat. Rev. Mol. Cell Biol."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Dos Santos, S.C., Teixeira, M.C., Dias, P.J., and Isabel, S.-C. (2014). MFS transporters required for multidrug\/multixenobiotic (MD\/MX) resistance in the model yeast: Understanding their physiological function through post-genomic approaches. Front. Physiol., 5.","DOI":"10.3389\/fphys.2014.00180"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"391","DOI":"10.1007\/s10930-018-9785-7","article-title":"WD40 repeat proteins: Signalling scaffold with diverse functions","volume":"37","author":"Jain","year":"2018","journal-title":"Protein J."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"980","DOI":"10.1016\/j.ijbiomac.2021.10.062","article-title":"Glycosyl hydrolases family 5, subfamily 5: Relevance and structural insights for designing improved biomass degrading cocktails","volume":"193","author":"Neis","year":"2021","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Fang, M., Wang, X., Chen, Y., Wang, P., Lu, L., Lu, J., Yao, F., and Zhang, Y. (2020). Genome Sequence Analysis of Auricularia heimuer Combined with Genetic Linkage Map. J. Fungi, 6.","DOI":"10.3390\/jof6010037"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Tyibilika, V., Setati, M.E., Bloem, A., Divol, B., and Camarasa, C. (2024). Differences in the management of intracellular redox state between wine yeast species dictate their fermentation performances and metabolite production. Int. J. Food Microbiol., 411.","DOI":"10.1016\/j.ijfoodmicro.2023.110537"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"56","DOI":"10.1016\/j.freeradbiomed.2017.12.024","article-title":"Redox and the circadian clock in plant immunity: A balancing act","volume":"119","author":"Karapetyan","year":"2018","journal-title":"Free Radic. Biol. Med."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1015","DOI":"10.1016\/j.bbabio.2018.05.012","article-title":"Online in vivo monitoring of cytosolic NAD redox dynamics in Ustilago maydis","volume":"1859","author":"Hartmann","year":"2018","journal-title":"Biochim. Biophys. Acta-Bioenerg."},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Vallieres, C., Golinelli-Cohen, M.-P., Guittet, O., Lepoivre, M., Huang, M.-E., and Vernis, L. (2023). Redox-based strategies against infections by eukaryotic pathogens. Genes, 14.","DOI":"10.3390\/genes14040778"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2913","DOI":"10.1093\/jxb\/erv073","article-title":"Nitric oxide is a ubiquitous signal for maintaining redox balance in plant cells: Regulation of ascorbate peroxidase as a case study","volume":"66","author":"Foresi","year":"2015","journal-title":"J. Exp. Bot."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"177396","DOI":"10.1016\/j.scitotenv.2024.177396","article-title":"The Keap1-Nrf2\/ARE signaling pathway regulates redox balance and apoptosis in the small yellow croaker (Larimichthys polyactis) under hypoxic stress","volume":"957","author":"Wang","year":"2024","journal-title":"Sci. Total Environ."},{"key":"ref_45","doi-asserted-by":"crossref","unstructured":"Moulis, J.-M. (2020). Cellular dynamics of transition metal exchange on proteins: A challenge but a bonanza for coordination chemistry. Biomolecules, 10.","DOI":"10.3390\/biom10111584"},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"Fones, H., Davis, C.A.R., Rico, A., Fang, F., Smith, J.A.C., and Preston, G.M. (2010). Metal hyperaccumulation armors plants against disease. PLoS Pathogens, 6.","DOI":"10.1371\/journal.ppat.1001093"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3292","DOI":"10.1021\/acscatal.5b00335","article-title":"Mo2C\/Graphene Nanocomposite As a Hydrodeoxygenation Catalyst for the Production of Diesel Range Hydrocarbons","volume":"5","author":"Kim","year":"2015","journal-title":"ACS Catal."},{"key":"ref_48","first-page":"1510","article-title":"Screening of reference genes for qRT-PCR amplification in Auricularia heimuer","volume":"39","author":"Zhang","year":"2020","journal-title":"Mycosystema"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/11\/5\/387\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:34:36Z","timestamp":1760031276000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/11\/5\/387"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,5,17]]},"references-count":48,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2025,5]]}},"alternative-id":["jof11050387"],"URL":"https:\/\/doi.org\/10.3390\/jof11050387","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,5,17]]}}}