{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T19:54:52Z","timestamp":1765828492059,"version":"build-2065373602"},"reference-count":55,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2025,6,13]],"date-time":"2025-06-13T00:00:00Z","timestamp":1749772800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Research Council of Thailand","award":["N21A660515","FFB680075\/0337","31st Science and Technology Research Grant","T2-65-05"],"award-info":[{"award-number":["N21A660515","FFB680075\/0337","31st Science and Technology Research Grant","T2-65-05"]}]},{"name":"National Science, Research, and Innovation Fund, Thailand Science, Research, and Innovation","award":["N21A660515","FFB680075\/0337","31st Science and Technology Research Grant","T2-65-05"],"award-info":[{"award-number":["N21A660515","FFB680075\/0337","31st Science and Technology Research Grant","T2-65-05"]}]},{"name":"Thailand Toray Science Foundation","award":["N21A660515","FFB680075\/0337","31st Science and Technology Research Grant","T2-65-05"],"award-info":[{"award-number":["N21A660515","FFB680075\/0337","31st Science and Technology Research Grant","T2-65-05"]}]},{"name":"Scholarship of TU-NSTDA Excellent Research Graduate Scholarship","award":["N21A660515","FFB680075\/0337","31st Science and Technology Research Grant","T2-65-05"],"award-info":[{"award-number":["N21A660515","FFB680075\/0337","31st Science and Technology Research Grant","T2-65-05"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The development of yeast cell factories for efficient xylose utilization and xylitol production is crucial for advancing sustainable biotechnological processes. Xylose, a major component of lignocellulosic biomass, presents challenges for microbial conversion due to its complex metabolic pathways. This study presents the genomic perspective and xylitol production capability of a novel xylose utilizing yeast Cyberlindnera fabianii TBRC 4498. Genome sequencing and functional annotation revealed key metabolic networks and genes involved in the xylose metabolism pathway, providing insights into the strain\u2019s performance. The Cy. fabianii TBRC 4498 had excellent growth and xylose assimilation at a broad range of xylose concentrations from 40 to 140 g\/L, with the highest growth rate at 80 g\/L of xylose. The highest xylitol production yield (83.19 g\/L) was detected from 120 g\/L of xylose at 30 \u00b0C for 72 h, equivalent to 0.65 g xylitol\/g xylose and 1.16 g\/L\/h productivity. Remarkably, Cy. fabianii TBRC 4498 produced high-purity xylitol, achieving over 95% homogeneity without forming undesirable byproducts, such as acid or ethanol. These results demonstrated the potential of Cy. fabianii TBRC 4498 as a whole-cell biocatalyst for xylitol production using high xylose concentrations, offering a promising microbial cell factory for large-scale xylitol production from lignocellulosic sugar.<\/jats:p>","DOI":"10.3390\/jof11060453","type":"journal-article","created":{"date-parts":[[2025,6,16]],"date-time":"2025-06-16T06:40:27Z","timestamp":1750056027000},"page":"453","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":2,"title":["Genomic and Functional Analysis of a Novel Yeast Cyberlindnera fabianii TBRC 4498 for High-Yield Xylitol Production"],"prefix":"10.3390","volume":"11","author":[{"given":"Pawarin","family":"Bonthong","sequence":"first","affiliation":[{"name":"Department of Biotechnology, Faculty of Science and Technology, Rangsit Campus, Thammasat University, Pathum Thani 12120, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6741-684X","authenticated-orcid":false,"given":"Benjarat","family":"Bunterngsook","sequence":"additional","affiliation":[{"name":"Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3355-0071","authenticated-orcid":false,"given":"Wuttichai","family":"Mhuantong","sequence":"additional","affiliation":[{"name":"Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Katesuda","family":"Aiewviriyasakul","sequence":"additional","affiliation":[{"name":"Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Wipawee","family":"Sritusnee","sequence":"additional","affiliation":[{"name":"Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Verawat","family":"Champreda","sequence":"additional","affiliation":[{"name":"Enzyme Technology Research Team, Biorefinery Technology and Bioproduct Research Group, National Center for Genetic Engineering and Biotechnology, 113 Thailand Science Park, Khlong Luang, Pathum Thani 12120, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9383-5098","authenticated-orcid":false,"given":"Hataikarn","family":"Lekakarn","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science and Technology, Rangsit Campus, Thammasat University, Pathum Thani 12120, Thailand"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,6,13]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"834","DOI":"10.7150\/ijbs.6.834","article-title":"Microbial and bioconversion production of D-xylitol and its detection and application","volume":"6","author":"Chen","year":"2010","journal-title":"Int. J. Biol. Sci."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Tiefenbacher, K.F. (2017). Technology of Main Ingredients\u2014Sweeteners and Lipids. Wafer Waffle, 123\u2013225.","DOI":"10.1016\/B978-0-12-809438-9.00003-X"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1514","DOI":"10.1080\/10408398.2012.702288","article-title":"Xylitol: A review on bioproduction, application, health benefits, and related safety issues","volume":"55","author":"Mushtaq","year":"2015","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"da Silva, S.S., and Chandel, A.K. (2012). Application of xylitol in food formulations and benefits for health. D-xylitol: Fermentative production, Application and Commercialization, Springer.","DOI":"10.1007\/978-3-642-31887-0"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.procbio.2018.03.023","article-title":"Effect of cosubstrate on xylitol production by Debaryomyces nepalensis NCYC 3413: A cybernetic modelling approach","volume":"69","author":"Pappu","year":"2018","journal-title":"Process Biochem."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"349","DOI":"10.1002\/yea.3394","article-title":"Xylitol production on sugarcane biomass hydrolysate by newly identified JA2 strain","volume":"36","author":"Morais","year":"2019","journal-title":"Yeast"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"919","DOI":"10.1007\/s10482-015-0546-8","article-title":"Xylitol production by yeasts isolated from rotting wood in the Gal\u00e1pagos Islands, Ecuador, and description of Cyberlindnera galapagoensis f.a., sp. nov","volume":"108","author":"Cadete","year":"2015","journal-title":"Anton. Leeuw. Int. J. G."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Carneiro, C.V.G.C., Silva, F.C.d.P.e., and Almeida, J.R.M. (2019). Xylitol production: Identification and comparison of new producing yeasts. Microorganisms, 7.","DOI":"10.3390\/microorganisms7110484"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"690","DOI":"10.1002\/bbb.2196","article-title":"Novel potential yeast strains for the biotechnological production of xylitol from sugarcane bagasse","volume":"15","author":"Palladino","year":"2021","journal-title":"Biofuel Bioprod. Biorefining"},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Kusumawati, N., Sumarlan, S.H., Zubaidah, E., and Wardani, A.K. (2023). Isolation of xylose-utilizing yeasts from oil palm waste for xylitol and ethanol production. Bioresour. Bioprocess., 10.","DOI":"10.1186\/s40643-023-00691-y"},{"key":"ref_11","first-page":"89","article-title":"Nutrient limitation leads to penetrative growth into agar and affects aroma formation in Pichia fabianii, P. kudriavzevii and Saccharomyces cerevisiae","volume":"32","author":"Dijksterhuis","year":"2015","journal-title":"Yeast"},{"key":"ref_12","first-page":"10","article-title":"Genome sequences of Cyberlindnera fabianii 65, Pichia kudriavzevii 129, and Saccharomyces cerevisiae 131 isolated from fermented masau fruits in Zimbabwe","volume":"5","author":"Derks","year":"2017","journal-title":"Genome Announc."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Liang, J., Yuan, H., Fei, Y., Wang, H., Qu, C., Bai, W., and Liu, G. (2024). Effects of Saccharomyces cerevisiae and Cyberlindnera fabianii inoculation on rice-flavor baijiu fermentation. Foods, 13.","DOI":"10.3390\/foods13193175"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"113","DOI":"10.1016\/j.fm.2019.04.010","article-title":"Dynamic modelling of brewers\u2019 yeast and Cyberlindnera fabianii co-culture behaviour for steering fermentation performance","volume":"83","author":"Pijffers","year":"2019","journal-title":"Food Microbiol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1402","DOI":"10.1002\/wer.1499","article-title":"Mycoremediation of azo dyes using Cyberlindnera fabianii yeast strain: Application of designs of experiments for decolorization optimization","volume":"93","author":"Danouche","year":"2021","journal-title":"Water Environ. Res."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Prjibelski, A., Antipov, D., Meleshko, D., Lapidus, A., and Korobeynikov, A. (2020). Using SPAdes De Novo Assembler. Curr. Protoc. Bioinform., 70.","DOI":"10.1002\/cpbi.102"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Alonge, M., Lebeigle, L., Kirsche, M., Jenike, K., Ou, S., Aganezov, S., Wang, X., Lippman, Z.B., Schatz, M.C., and Soyk, S. (2022). Automated assembly scaffolding using RagTag elevates a new tomato system for high-throughput genome editing. Genome Biol., 23.","DOI":"10.1186\/s13059-022-02823-7"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1072","DOI":"10.1093\/bioinformatics\/btt086","article-title":"QUAST: Quality assessment tool for genome assemblies","volume":"29","author":"Gurevich","year":"2013","journal-title":"Bioinformatics"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Manni, M., Berkeley, M.R., Seppey, M., and Zdobnov, E.M. (2021). BUSCO: Assessing genomic data quality and beyond. Curr. Protoc., 1.","DOI":"10.1002\/cpz1.323"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"W465","DOI":"10.1093\/nar\/gki458","article-title":"AUGUSTUS: A web server for gene prediction in eukaryotes that allows user-defined constraints","volume":"33","author":"Stanke","year":"2005","journal-title":"Nucleic Acids Res."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Haas, B.J., Salzberg, S.L., Zhu, W., Pertea, M., Allen, J.E., Orvis, J., White, O., Buell, C.R., and Wortman, J.R. (2008). Automated eukaryotic gene structure annotation using EVidenceModeler and the program to assemble spliced alignments. Genome Biol., 9.","DOI":"10.1186\/gb-2008-9-1-r7"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"D523","DOI":"10.1093\/nar\/gkac1052","article-title":"UniProt: The Universal Protein Knowledgebase in 2023","volume":"51","author":"Consortium","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"W95","DOI":"10.1093\/nar\/gky418","article-title":"dbCAN2: A meta server for automated carbohydrate-active enzyme annotation","volume":"46","author":"Zhang","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"726","DOI":"10.1016\/j.jmb.2015.11.006","article-title":"BlastKOALA and GhostKOALA: KEGG tools for functional characterization of genome and metagenome sequences","volume":"428","author":"Kanehisa","year":"2016","journal-title":"J. Mol. Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"W84","DOI":"10.1093\/nar\/gky350","article-title":"PANNZER2: A rapid functional annotation web server","volume":"46","author":"Medlar","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_27","first-page":"D309","article-title":"eggNOG 5.0: A hierarchical, functionally and phylogenetically annotated orthology resource based on 5090 organisms and 2502 viruses","volume":"47","author":"Szklarczyk","year":"2018","journal-title":"Nucleic Acids Res."},{"key":"ref_28","first-page":"D222","article-title":"CDD: NCBI\u2019s conserved domain database","volume":"43","author":"Derbyshire","year":"2014","journal-title":"Nucleic Acids Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1023","DOI":"10.1038\/s41587-021-01156-3","article-title":"SignalP 6.0 predicts all five types of signal peptides using protein language models","volume":"40","author":"Teufel","year":"2022","journal-title":"Nat. Biotechnol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1111\/2041-210X.12073","article-title":"Improved software detection and extraction of ITS1 and ITS2 from ribosomal ITS sequences of fungi and other eukaryotes for analysis of environmental sequencing data","volume":"4","author":"Ryberg","year":"2013","journal-title":"Methods Ecol. Evol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"5114","DOI":"10.1038\/s41467-018-07641-9","article-title":"High throughput ANI analysis of 90K prokaryotic genomes reveals clear species boundaries","volume":"9","author":"Jain","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"D777","DOI":"10.1093\/nar\/gkac894","article-title":"UFCG: Database of universal fungal core genes and pipeline for genome-wide phylogenetic analysis of fungi","volume":"51","author":"Kim","year":"2022","journal-title":"Nucleic Acids Res."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"473","DOI":"10.5248\/110.473","article-title":"Cyberlindnera, a replacement name for Lindnera Kurtzman et al., nom. illegit","volume":"110","author":"Minter","year":"2009","journal-title":"Mycotaxon"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"939","DOI":"10.1111\/j.1567-1364.2008.00419.x","article-title":"Phylogenetic relationships among species of Pichia, Issatchenkia and Williopsis determined from multigene sequence analysis, and the proposal of Barnettozyma gen. nov., Lindnera gen. nov. and Wickerhamomyces gen. nov","volume":"8","author":"Kurtzman","year":"2008","journal-title":"FEMS Yeast Res."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Bellut, K., Michel, M., Zarnkow, M., Hutzler, M., Jacob, F., Atzler, J.J., Hoehnel, A., Lynch, K.M., and Arendt, E.K. (2019). Screening and application of Cyberlindnera yeasts to produce a fruity, non-alcoholic beer. Fermentation, 5.","DOI":"10.3390\/fermentation5040103"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"914","DOI":"10.1002\/cche.10670","article-title":"Impact of solid-state fermentation by Aspergillus niger 4Q and Cyberlindnera fabianii J2 on antioxidant and flavor properties of whole grain barley","volume":"100","author":"Zhai","year":"2023","journal-title":"Cereal Chem."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Sousa-Silva, M., Vieira, D., Soares, P., Casal, M., and Soares-Silva, I. (2021). Expanding the knowledge on the skillful yeast Cyberlindnera jadinii. J. Fungi, 7.","DOI":"10.3390\/jof7010036"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Freel, K.C., Sarilar, V., Neuveglise, C., Devillers, H., Friedrich, A., and Schacherer, J. (2014). Genome sequence of the yeast Cyberlindnera fabianii (Hansenula fabianii). Genome Announc., 2.","DOI":"10.1128\/genomeA.00638-14"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"818","DOI":"10.1002\/bit.10631","article-title":"Control of xylose consumption by xylose transport in recombinant Saccharomyces cerevisiae","volume":"82","author":"Jeppsson","year":"2003","journal-title":"Biotechnol. Bioeng."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"511","DOI":"10.1111\/j.1567-1364.2009.00509.x","article-title":"Hexose and pentose transport in ascomycetous yeasts: An overview","volume":"9","author":"Leandro","year":"2009","journal-title":"FEMS Yeast Res."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Bueno, J.G.R., Borelli, G., Corr\u00eaa, T.L.R., Fiamenghi, M.B., Jos\u00e9, J., de Carvalho, M., de Oliveira, L.C., Pereira, G.A.G., and dos Santos, L.V. (2020). Novel xylose transporter Cs4130 expands the sugar uptake repertoire in recombinant Saccharomyces cerevisiae strains at high xylose concentrations. Biotechnol. Biofuels, 13.","DOI":"10.1186\/s13068-020-01782-0"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"447","DOI":"10.1007\/s002530050954","article-title":"Effect of xylitol and trehalose on dry resistance of yeasts","volume":"47","author":"Krallish","year":"1997","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2059","DOI":"10.1111\/1751-7915.13881","article-title":"Production of xylitol by Saccharomyces cerevisiae using waste xylose mother liquor and corncob residues","volume":"14","author":"He","year":"2021","journal-title":"Microb. Biotechnol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Umai, D., Kayalvizhi, R., Kumar, V., and Jacob, S. (2022). Xylitol: Bioproduction and applications\u2014A review. Front. Sustain., 3.","DOI":"10.3389\/frsus.2022.826190"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"3322","DOI":"10.1016\/j.biortech.2010.10.111","article-title":"Development of a yeast strain for xylitol production without hydrolysate detoxification as part of the integration of co-product generation within the lignocellulosic ethanol process","volume":"102","author":"Huang","year":"2011","journal-title":"Bioresour. Technol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"34","DOI":"10.3109\/10242422.2013.870558","article-title":"Optimization of D-xylose to xylitol biotransformation by Candida guilliermondii cells permeabilized with Triton X-100","volume":"32","author":"Cortez","year":"2013","journal-title":"Biocatal. Biotransform."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"7930","DOI":"10.1021\/jf034426j","article-title":"Multiple forms of xylose reductase in Candida intermedia: Comparison of their functional properties using quantitative structure-activity relationships, steady-state kinetic analysis, and pH studies","volume":"51","author":"Nidetzky","year":"2003","journal-title":"J. Agric. Food Chem."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Zha, J., Yuwen, M., Qian, W., and Wu, X. (2021). Yeast-based biosynthesis of natural products from xylose. Front. Bioeng. Biotechnol., 9.","DOI":"10.3389\/fbioe.2021.634919"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1038\/nbt1290","article-title":"Genome sequence of the lignocellulose-bioconverting and xylose-fermenting yeast Pichia stipitis","volume":"25","author":"Jeffries","year":"2007","journal-title":"Nat. Biotechnol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1357","DOI":"10.1111\/jam.12327","article-title":"Xylitol production by Cyberlindnera (Williopsis) saturnus, a tropical mangrove yeast from xylose and corn cob hydrolysate","volume":"115","author":"Kamat","year":"2013","journal-title":"J. Appl. Microbiol."},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1385\/ABAB:98-100:1-9:449","article-title":"Influence of oxygen availability on cell growth and xylitol production by Candida guilliermondii","volume":"98","author":"Faria","year":"2002","journal-title":"Appl. Biochem. Biotechnol."},{"key":"ref_52","first-page":"3037","article-title":"Molecular identification of novel yeast isolate associated with xylitol production and its physicochemical parameters optimization","volume":"8","author":"Rengaraju","year":"2014","journal-title":"J. Pure Appl. Microbiol."},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"387","DOI":"10.1002\/jctb.2732","article-title":"Xylitol production by Candida tropicalis from corn cob hemicellulose hydrolysate in a two-stage fed-batch fermentation process","volume":"87","author":"Li","year":"2012","journal-title":"J. Chem. Technol. Biotechnol."},{"key":"ref_54","unstructured":"Neeru, C., Chandrajit, B., and Vidyasagar, J. (2013). Biological production of xylitol from corn husk and switchgrass by Pichia stiptis. Res. J. Chem. Sci., 2231."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.cattod.2014.10.054","article-title":"Xylitol production from cashew apple bagasse by Kluyveromyces marxianus CCA510","volume":"255","author":"Gomes","year":"2015","journal-title":"Catal. Today"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/11\/6\/453\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T17:51:51Z","timestamp":1760032311000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/11\/6\/453"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,6,13]]},"references-count":55,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2025,6]]}},"alternative-id":["jof11060453"],"URL":"https:\/\/doi.org\/10.3390\/jof11060453","relation":{},"ISSN":["2309-608X"],"issn-type":[{"type":"electronic","value":"2309-608X"}],"subject":[],"published":{"date-parts":[[2025,6,13]]}}}