{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,18]],"date-time":"2026-08-18T04:52:01Z","timestamp":1787028721013,"version":"build-2736575974"},"reference-count":48,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2025,7,12]],"date-time":"2025-07-12T00:00:00Z","timestamp":1752278400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Colombia\u2019s Ministry of Science, Technology and Innovation (MIN-CIENCIAS)"},{"name":"School of Medicine and Health Sciences (ABN122), Universidad del Rosario, Bogot\u00e1, Colombia"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The number of fungal infections is steadily increasing, with considerable morbidity and mortality. Additionally, antifungal resistance is a growing concern, highlighting the need to develop new treatment options. One alternative is the use of antimicrobial peptides (AMPs). The aim of this study was to assess the in vitro and in vivo antifungal activity of designed short AMPs, Act-6 and Act 8-20, derived from cecropin transcripts of beetles from the family Scarabaeidae, against eight reference strains of the pathogenic yeasts Candida and Cryptococcus. We also evaluated the effect of these modified AMPs on the biofilm, morphogenesis, and cell morphology of Candida albicans, as well as the in vivo activity via a murine model of disseminated candidiasis. The AMPs herein analyzed exhibit differential antifungal activity against the yeasts assessed, and inhibit biofilm, hyphae, and pseudohyphae formation with morphological alterations in C. albicans. Moreover, the fungal load in mice treated with these AMPs significantly decreased. Altogether, our results suggest that Act-6 and Act 8-20 are promising antifungal molecules to control mycoses.<\/jats:p>","DOI":"10.3390\/jof11070519","type":"journal-article","created":{"date-parts":[[2025,7,14]],"date-time":"2025-07-14T10:55:41Z","timestamp":1752490541000},"page":"519","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Antimicrobial Peptides Act-6 and Act 8-20 Derived from Scarabaeidae Cecropins Exhibit Differential Antifungal Activity"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7713-3598","authenticated-orcid":false,"given":"Melissa","family":"Rodr\u00edguez","sequence":"first","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, Translational Medicine Institute, School of Medicine and Health Sciences, Universidad del Rosario, Bogota 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7906-1997","authenticated-orcid":false,"given":"Lily J.","family":"Toro","sequence":"additional","affiliation":[{"name":"Center of Biomedical Research, Group of Molecular Immunology, Universidad del Quind\u00edo, Armenia 630001, Quind\u00edo, Colombia"},{"name":"Health Knowledge Management Research Group, Medical Science Faculty, Corporaci\u00f3n Universitaria Empresarial Alexander Von Humboldt (UNIHUMBOLDT), Armenia 630004, Quind\u00edo, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7547-5172","authenticated-orcid":false,"given":"Carolina","family":"Firacative","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, Translational Medicine Institute, School of Medicine and Health Sciences, Universidad del Rosario, Bogota 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6641-1924","authenticated-orcid":false,"given":"Beatriz L.","family":"G\u00f3mez","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, Translational Medicine Institute, School of Medicine and Health Sciences, Universidad del Rosario, Bogota 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1521-1519","authenticated-orcid":false,"given":"Bruno","family":"Rivas-Santiago","sequence":"additional","affiliation":[{"name":"Biomedical Research Unit-Zacatecas, Mexican Institute for Social Security-IMSS, Zacatecas 98000, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6317-6666","authenticated-orcid":false,"given":"David","family":"Andreu","sequence":"additional","affiliation":[{"name":"Proteomics and Protein Chemistry Unit, Department of Medicine and Life Sciences, Pompeu Fabra University, Barcelona Biomedical Research Park, Dr. Aiguader 88, 08003 Barcelona, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7639-3053","authenticated-orcid":false,"given":"Jhon C.","family":"Casta\u00f1o","sequence":"additional","affiliation":[{"name":"Center of Biomedical Research, Group of Molecular Immunology, Universidad del Quind\u00edo, Armenia 630001, Quind\u00edo, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3057-9290","authenticated-orcid":false,"given":"German A.","family":"T\u00e9llez","sequence":"additional","affiliation":[{"name":"Center of Biomedical Research, Group of Molecular Immunology, Universidad del Quind\u00edo, Armenia 630001, Quind\u00edo, Colombia"},{"name":"Health Knowledge Management Research Group, Medical Science Faculty, Corporaci\u00f3n Universitaria Empresarial Alexander Von Humboldt (UNIHUMBOLDT), Armenia 630004, Quind\u00edo, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4495-7077","authenticated-orcid":false,"given":"Juli\u00e1n E.","family":"Mu\u00f1oz","sequence":"additional","affiliation":[{"name":"Studies in Translational Microbiology and Emerging Diseases (MICROS) Research Group, Translational Medicine Institute, School of Medicine and Health Sciences, Universidad del Rosario, Bogota 111221, Colombia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"18026","DOI":"10.1038\/nrdp.2018.26","article-title":"Invasive Candidiasis","volume":"4","author":"Pappas","year":"2018","journal-title":"Nat. Rev. Dis. Primers"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"389","DOI":"10.1016\/j.idc.2021.03.007","article-title":"Candidemia and Invasive Candidiasis","volume":"35","author":"McCarty","year":"2021","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Bongomin, F., Gago, S., Oladele, R., and Denning, D. (2017). Global and Multi-National Prevalence of Fungal Diseases\u2014Estimate Precision. J. Fungi, 3.","DOI":"10.3390\/jof3040057"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"42","DOI":"10.2174\/1871526515666150320162036","article-title":"Candidiasis: A Fungal Infection- Current Challenges and Progress in Prevention and Treatment","volume":"15","author":"Hani","year":"2015","journal-title":"Infect. Disord.-Drug Targets"},{"key":"ref_5","first-page":"39","article-title":"Enfoque Cl\u00ednico Del S\u00edndrome Febril Agudo En Colombia","volume":"21","author":"Cuervo","year":"2017","journal-title":"Infectio"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1038\/s41572-024-00503-3","article-title":"Invasive Candidiasis","volume":"10","author":"Kanj","year":"2024","journal-title":"Nat. Rev. Dis. Primer"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Ben-Ami, R. (2018). Treatment of Invasive Candidiasis: A Narrative Review. J. Fungi, 4.","DOI":"10.3390\/jof4030097"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"792","DOI":"10.1016\/j.cmi.2019.03.028","article-title":"Resistance of Candida to Azoles and Echinocandins Worldwide","volume":"25","author":"Pristov","year":"2019","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Carmo, A., Rocha, M., Pereirinha, P., Tom\u00e9, R., and Costa, E. (2023). Antifungals: From Pharmacokinetics to Clinical Practice. Antibiotics, 12.","DOI":"10.3390\/antibiotics12050884"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"317","DOI":"10.1016\/j.tim.2004.05.008","article-title":"The Distinct Morphogenic States of Candida albicans","volume":"12","author":"Sudbery","year":"2004","journal-title":"Trends Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"179","DOI":"10.1016\/j.idc.2015.10.006","article-title":"Cryptococcosis","volume":"30","author":"Maziarz","year":"2016","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1748","DOI":"10.1016\/S1473-3099(22)00499-6","article-title":"The Global Burden of HIV-Associated Cryptococcal Infection in Adults in 2020: A Modelling Analysis","volume":"22","author":"Rajasingham","year":"2022","journal-title":"Lancet Infect. Dis."},{"key":"ref_13","unstructured":"World Health Organization (2022). Guidelines for Diagnosing, Preventing and Managing Cryptococcal Disease Among Adults, Adolescents and Children Living with HIV."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"e170391","DOI":"10.1590\/0074-02760170391","article-title":"The War on Cryptococcosis: A Review of the Antifungal Arsenal","volume":"113","author":"Mourad","year":"2018","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Melhem, M.S.C., Leite J\u00fanior, D.P., Takahashi, J.P.F., Macioni, M.B., Oliveira, L.D., De Ara\u00fajo, L.S., Fava, W.S., Bonfietti, L.X., Paniago, A.M.M., and Venturini, J. (2024). Antifungal Resistance in Cryptococcal Infections. Pathogens, 13.","DOI":"10.3390\/pathogens13020128"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Huan, Y., Kong, Q., Mou, H., and Yi, H. (2020). Antimicrobial Peptides: Classification, Design, Application and Research Progress in Multiple Fields. Front. Microbiol., 11.","DOI":"10.3389\/fmicb.2020.582779"},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Luo, Y., and Song, Y. (2021). Mechanism of Antimicrobial Peptides: Antimicrobial, Anti-Inflammatory and Antibiofilm Activities. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms222111401"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1155\/2012\/101989","article-title":"Coleopteran Antimicrobial Peptides: Prospects for Clinical Applications","volume":"2012","author":"Ntwasa","year":"2012","journal-title":"Int. J. Microbiol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"170626","DOI":"10.1016\/j.peptides.2021.170626","article-title":"Novel Antimicrobial Cecropins Derived from O. curvicornis and D. satanas Dung Beetles","volume":"145","author":"Toro","year":"2021","journal-title":"Peptides"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1093\/jisesa\/ieab054","article-title":"New Insect Host Defense Peptides (HDP) From Dung Beetle (Coleoptera: Scarabaeidae) Transcriptomes","volume":"21","author":"Toro","year":"2021","journal-title":"J. Insect Sci."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"381","DOI":"10.4014\/jmb.1711.11057","article-title":"Design and Engineering of Antimicrobial Peptides Based on LPcin-YK3, an Antimicrobial Peptide Derivative from Bovine Milk","volume":"28","author":"Kim","year":"2018","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1686","DOI":"10.1002\/anie.202006372","article-title":"The Challenge of Peptide Proteolytic Stability Studies: Scarce Data, Difficult Readability, and the Need for Harmonization","volume":"60","author":"Cavaco","year":"2021","journal-title":"Angew. Chem. Int. Ed. Engl."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1349","DOI":"10.1111\/cts.12985","article-title":"Estimating Peptide Half-Life in Serum from Tunable, Sequence-Related Physicochemical Properties","volume":"14","author":"Cavaco","year":"2021","journal-title":"Clin. Transl. Sci."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1111\/j.1399-3011.1999.tb01620.x","article-title":"Structure-antibacterial, Antitumor and Hemolytic Activity Relationships of Cecropin A-magainin 2 and Cecropin A-melittin Hybrid Peptides","volume":"53","author":"Shin","year":"1999","journal-title":"J. Pept. Res."},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Flores-Alvarez, L.J., Jim\u00e9nez-Alc\u00e1ntar, P., Ochoa-Zarzosa, A., and L\u00f3pez-Meza, J.E. (2023). The Antimicrobial Peptide \u03b3-Thionin from Habanero Chile (Capsicum Chinense) Induces Caspase-Independent Apoptosis on Human K562 Chronic Myeloid Leukemia Cells and Regulates Epigenetic Marks. Molecules, 28.","DOI":"10.3390\/molecules28093661"},{"key":"ref_26","unstructured":"Clinical and Laboratory Standards Institute (CLSI) (2022). Performance Standards for Antifungal Susceptibility Testing of Yeasts Guidelines for Diagnosing, Clinical and Laboratory Standards Institute. [3rd ed.]."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Spampinato, C., and Leonardi, D. (2013). Candida Infections, Causes, Targets, and Resistance Mechanisms: Traditional and Alternative Antifungal Agents. BioMed Res. Int., 2013.","DOI":"10.1155\/2013\/204237"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1494","DOI":"10.1038\/nprot.2008.141","article-title":"A Simple and Reproducible 96-Well Plate-Based Method for the Formation of Fungal Biofilms and Its Application to Antifungal Susceptibility Testing","volume":"3","author":"Pierce","year":"2008","journal-title":"Nat. Protoc."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Mu\u00f1oz, J.E., Rossi, D.C.P., Ishida, K., Spadari, C.C., Melhem, M.S.C., Garcia, D.M., Caires, A.C.F., Taborda, C.P., and Rodrigues, E.G. (2017). Antifungal Activity of the Biphosphinic Cyclopalladate C7a against Candida albicans Yeast Forms In Vitro and In Vivo. Front. Microbiol., 8.","DOI":"10.3389\/fmicb.2017.00771"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Torres, R., Barreto-Santamar\u00eda, A., Ar\u00e9valo-Pinz\u00f3n, G., Firacative, C., G\u00f3mez, B.L., Escand\u00f3n, P., Patarroyo, M.A., and Mu\u00f1oz, J.E. (2023). In Vitro Antifungal Activity of Three Synthetic Peptides against Candida auris and Other Candida Species of Medical Importance. Antibiotics, 12.","DOI":"10.3390\/antibiotics12081234"},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Rossi, D.C., Mu\u00f1oz, J.E., Carvalho, D.D., Belmonte, R., Faintuch, B., Borelli, P., Miranda, A., Taborda, C.P., and Daffre, S. (2012). Therapeutic Use of a Cationic Antimicrobial Peptide from the Spider Acanthoscurria gomesiana in the Control of Experimental Candidiasis. BMC Microbiol., 12.","DOI":"10.1186\/1471-2180-12-28"},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Dutta, P., Sahu, R.K., Dey, T., Lahkar, M.D., Manna, P., and Kalita, J. (2019). Beneficial Role of Insect-Derived Bioactive Components against Inflammation and Its Associated Complications (Colitis and Arthritis) and Cancer. Chem. Biol. Interact., 313.","DOI":"10.1016\/j.cbi.2019.108824"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"6497","DOI":"10.1111\/j.1742-4658.2009.07360.x","article-title":"Multifunctional Host Defense Peptides: Antimicrobial Peptides, the Small yet Big Players in Innate and Adaptive Immunity","volume":"276","author":"Auvynet","year":"2009","journal-title":"FEBS J."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"35","DOI":"10.2174\/1389203053027494","article-title":"A Re-Evaluation of the Role of Host Defence Peptides in Mammalian Immunity","volume":"6","author":"Bowdish","year":"2005","journal-title":"Curr. Protein Pept. Sci."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Toro Segovia, L.J., T\u00e9llez Ram\u00edrez, G.A., Henao Arias, D.C., Rivera Duran, J.D., Bedoya, J.P., and Casta\u00f1o Osorio, J.C. (2017). Identification and Characterization of Novel Cecropins from the Oxysternon conspicillatum Neotropic Dung Beetle. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0187914"},{"key":"ref_36","first-page":"11786337211026927","article-title":"Candida Bloodstream Infections: Changes in Epidemiology and Increase in Drug Resistance","volume":"14","author":"Kotey","year":"2021","journal-title":"Infect. Dis. Res. Treat."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"759","DOI":"10.4014\/jmb.1609.09004","article-title":"Design, Characterization, and Antimicrobial Activity of a Novel Antimicrobial Peptide Derived from Bovine Lactophoricin","volume":"27","author":"Kim","year":"2017","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/s12275-016-5514-0","article-title":"Interaction of Candida albicans with Host Cells: Virulence Factors, Host Defense, Escape Strategies, and the Microbiota","volume":"54","author":"Mogavero","year":"2016","journal-title":"J. Microbiol."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"310","DOI":"10.1016\/j.micinf.2016.01.002","article-title":"Candida albicans Biofilms: Development, Regulation, and Molecular Mechanisms","volume":"18","author":"Gulati","year":"2016","journal-title":"Microbes Infect."},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Pinilla, G., Coronado, Y.T., Chaves, G., Mu\u00f1oz, L., Navarrete, J., Salazar, L.M., Taborda, C.P., and Mu\u00f1oz, J.E. (2022). In Vitro Antifungal Activity of LL-37 Analogue Peptides against Candida Spp. J. Fungi, 8.","DOI":"10.3390\/jof8111173"},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Sun, C., Zhao, X., Jiao, Z., Peng, J., Zhou, L., Yang, L., Huang, M., Tian, C., and Guo, G. (2022). The Antimicrobial Peptide AMP-17 Derived from Musca Domestica Inhibits Biofilm Formation and Eradicates Mature Biofilm in Candida albicans. Antibiotics, 11.","DOI":"10.3390\/antibiotics11111474"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1007\/s10096-011-1273-3","article-title":"Candida and Invasive Candidiasis: Back to Basics","volume":"31","author":"Lim","year":"2012","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"2509","DOI":"10.2147\/IDR.S250278","article-title":"Antimicrobial Peptide AMP-17 Affects Candida albicans by Disrupting Its Cell Wall and Cell Membrane Integrity","volume":"13","author":"Ma","year":"2020","journal-title":"Infect. Drug Resist."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Polonelli, L., Ciociola, T., Elviri, L., Zanello, P.P., Giovati, L., Arruda, D.C., Mu\u00f1oz, J.E., Mortara, R.A., Morace, G., and Borghi, E. (2016). A Naturally Occurring Antibody Fragment Neutralizes Infectivity of Diverse Infectious Agents. Sci. Rep., 6.","DOI":"10.1038\/srep35018"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"e3306","DOI":"10.1002\/psc.3306","article-title":"The Effect of Structural Modification of Antimicrobial Peptides on Their Antimicrobial Activity, Hemolytic Activity, and Plasma Stability","volume":"27","author":"Wang","year":"2021","journal-title":"J. Pept. Sci."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"9421","DOI":"10.1021\/acs.jmedchem.0c00583","article-title":"Rational Avoidance of Protease Cleavage Sites and Symmetrical End-Tagging Significantly Enhances the Stability and Therapeutic Potential of Antimicrobial Peptides","volume":"63","author":"Zhu","year":"2020","journal-title":"J. Med. Chem."},{"key":"ref_47","doi-asserted-by":"crossref","unstructured":"Yan, T., Li, F., Li, J., and Chen, F. (2021). Antifungal Activity of ToAP2D Peptide Against Sporothrix globosa. Front. Bioeng. Biotechnol., 9.","DOI":"10.3389\/fbioe.2021.761518"},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"881","DOI":"10.1128\/AAC.02624-15","article-title":"Development and In Vivo Evaluation of a Novel Histatin-5 Bioadhesive Hydrogel Formulation against Oral Candidiasis","volume":"60","author":"Kong","year":"2016","journal-title":"Antimicrob. Agents Chemother."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/11\/7\/519\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T18:09:04Z","timestamp":1760033344000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/11\/7\/519"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,7,12]]},"references-count":48,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2025,7]]}},"alternative-id":["jof11070519"],"URL":"https:\/\/doi.org\/10.3390\/jof11070519","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2025,7,12]]}}}