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In the present study, we investigated the biocontrol efficacy of eight native isolates of Trichoderma spp. against the soilborne phytopathogens Sclerotinia\u00a0sclerotiorum and Rhizoctonia\u00a0solani and a representative of the Mucoromycota, Phycomyces\u00a0blakesleeanus. An in vitro dual culture test showed a complete (100%) inhibition of S. sclerotiorum and P. blakesleeanus by each tested Trichoderma strain and a high (80\u2013100%) inhibition of R. solani. The crude chloroform extracts, whose peptide contents were confirmed by thin-layer chromatography, caused a concentration-dependent reduction in the growth of the target fungi, with inhibition comparable to the effect of the peptaibol standard alamethicin. Despite the differences between fungi from the phyla Basidiomycota, Ascomycota, and Mucoromycota, their inhibition by alamethicin followed the same dose\u2013response dependence. The growth inhibition of P. blakesleeanus induced by Trichoderma extracts was characterized by a significantly increased activity of antioxidative defense enzymes. Both variants of biocontrol agents, the native strains of Trichoderma spp. and their extracts, are efficient in controlling fungal growth and should be considered for the development of new potent bioformulations applicable in agriculture.<\/jats:p>","DOI":"10.3390\/jof11070535","type":"journal-article","created":{"date-parts":[[2025,7,18]],"date-time":"2025-07-18T11:12:34Z","timestamp":1752837154000},"page":"535","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":9,"title":["Biocontrol Potential of Native Trichoderma Strains Toward Soil-Borne Phytopathogenic and Saprotrophic Fungi"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4975-3471","authenticated-orcid":false,"given":"Kristina","family":"Atlagi\u0107","sequence":"first","affiliation":[{"name":"University of Belgrade\u2014Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1676-7508","authenticated-orcid":false,"given":"Tijana","family":"Cveti\u0107 Anti\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade\u2014Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8173-713X","authenticated-orcid":false,"given":"Jovana","family":"Luki\u010di\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade\u2014Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4703-3016","authenticated-orcid":false,"given":"Katarina","family":"Kru\u0161\u010di\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade\u2014Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0314-5032","authenticated-orcid":false,"given":"Miroslav","family":"\u017divi\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade\u2014Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8872-2099","authenticated-orcid":false,"given":"Nikola","family":"Unkovi\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade\u2014Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3794-7655","authenticated-orcid":false,"given":"Tanja","family":"Paji\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade\u2014Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9733-2367","authenticated-orcid":false,"given":"Katarina","family":"Stevanovi\u0107","sequence":"additional","affiliation":[{"name":"University of Belgrade\u2014Faculty of Biology, Studentski trg 16, 11158 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2626-0794","authenticated-orcid":false,"given":"Nata\u0161a V.","family":"Todorovi\u0107","sequence":"additional","affiliation":[{"name":"Institute for Biological Research \u201cSini\u0161a Stankovi\u0107\u201d, National Institute of the Republic of Serbia, University of Belgrade, Bulevar Despota Stefana 142, 11000 Belgrade, Serbia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,7,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/bs.af2s.2024.07.010","article-title":"Chapter Three\u2014Pesticides in Agriculture: Food Security vs. Food Safety","volume":"9","author":"Sharma","year":"2024","journal-title":"Advances in Food Security and Sustainability"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Manoharachary, C., Singh, H.B., and Varma, A. 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