{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:23:17Z","timestamp":1789431797125,"version":"build-2803163510"},"reference-count":71,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,1]],"date-time":"2025-08-01T00:00:00Z","timestamp":1754006400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Instituto Polit\u00e9cnico Nacional, Mexico","award":["CBF-2025-I-2871"],"award-info":[{"award-number":["CBF-2025-I-2871"]}]},{"name":"Instituto Polit\u00e9cnico Nacional, Mexico","award":["2025-07"],"award-info":[{"award-number":["2025-07"]}]},{"name":"Bordallo-Landa-Guill\u00e9n Foundation","award":["CBF-2025-I-2871"],"award-info":[{"award-number":["CBF-2025-I-2871"]}]},{"name":"Bordallo-Landa-Guill\u00e9n Foundation","award":["2025-07"],"award-info":[{"award-number":["2025-07"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Candidozyma auris is a multidrug-resistant, thermo- and osmotolerant yeast capable of persisting on biotic and abiotic surfaces, attributes likely linked to its cell wall composition. Here, seven putative genes encoding yapsins, aspartyl proteases GPI-anchored to the membrane or cell wall, were identified in the genomes of C. auris CJ97 and 20-1498, from clades III and IV, respectively. The C. auris YPS1 gene is orthologous to the SAP9 of C. albicans. The YPS7 gene is orthologous to YPS7 in C. glabrata and S. cerevisiae, so that they may share similar roles. An in silico analysis suggested an interaction between pepstatin and the catalytic domain of Yps1 and Yps7. Although this inhibitor, when combined with caffeine, had a subtle effect on the growth of C. auris, it induced alterations in the cell wall. CauYPS1 and CauYPS7 expression increased under nutrient starvation and NaCl, and at 42 \u00b0C. The transcriptome of the 20-1498 strain suggests that autophagy may play a role in thermal stress, probably degrading deleterious proteins or maintaining cell wall and vacuolar homeostasis. Therefore, CauYps1 and CauYps7 may play a role in the cell wall integrity of C. auris in stress conditions, and they could be a target of new antifungal or antivirulence agents.<\/jats:p>","DOI":"10.3390\/jof11080573","type":"journal-article","created":{"date-parts":[[2025,8,5]],"date-time":"2025-08-05T08:46:55Z","timestamp":1754383615000},"page":"573","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["The GPI-Anchored Aspartyl Proteases Encoded by the YPS1 and YPS7 Genes of Candidozyma auris and Their Role Under Stress Conditions"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-8100-3080","authenticated-orcid":false,"given":"Alvaro","family":"Vidal-Montiel","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de Bacterias y Levaduras, Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Prol. de Carpio y Plan de Ayala. Col. Sto. Tom\u00e1s, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daniel","family":"Clark-Flores","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de Bacterias y Levaduras, Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Prol. de Carpio y Plan de Ayala. Col. Sto. Tom\u00e1s, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7895-9460","authenticated-orcid":false,"given":"Eulogio","family":"Valent\u00edn-G\u00f3mez","sequence":"additional","affiliation":[{"name":"Departamento de Microbiolog\u00eda y Ecolog\u00eda, Universidad de Valencia, 46100 Burjassot, Valencia, Spain"},{"name":"Grupo de Investigaci\u00f3n Infecci\u00f3n Grave, Instituto de Investigaci\u00f3n La Fe, 46026 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3602-8000","authenticated-orcid":false,"given":"Juan Pedro","family":"Luna-Arias","sequence":"additional","affiliation":[{"name":"Departamento de Biolog\u00eda Celular, Centro de Investigaci\u00f3n y de Estudios Avanzados del Instituto Polit\u00e9cnico Nacional (Cinvestav-IPN), Mexico City 07360, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Erika","family":"Rosales-Cruz","sequence":"additional","affiliation":[{"name":"Laboratorio de Investigaci\u00f3n en Hematopatolog\u00eda, Departamento de Morfolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"C\u00e9sar","family":"Hern\u00e1ndez-Rodr\u00edguez","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de Bacterias y Levaduras, Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Prol. de Carpio y Plan de Ayala. Col. Sto. Tom\u00e1s, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0055-2991","authenticated-orcid":false,"given":"Lourdes","family":"Villa-Tanaca","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de Bacterias y Levaduras, Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Prol. de Carpio y Plan de Ayala. Col. Sto. Tom\u00e1s, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margarita","family":"Ju\u00e1rez-Montiel","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular de Bacterias y Levaduras, Departamento de Microbiolog\u00eda, Escuela Nacional de Ciencias Biol\u00f3gicas, Instituto Polit\u00e9cnico Nacional, Prol. de Carpio y Plan de Ayala. Col. Sto. Tom\u00e1s, Mexico City 11340, Mexico"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1997","DOI":"10.1099\/00221287-147-8-1997","article-title":"Candida albicans proteinases: Resolving the mystery of a gene family","volume":"147","author":"Hube","year":"2001","journal-title":"Microbiology"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"688","DOI":"10.1074\/jbc.M509297200","article-title":"Glycosylphosphatidylinositol-anchored proteases of Candida albicans target proteins necessary for both cellular processes and host-pathogen interactions","volume":"281","author":"Albrecht","year":"2006","journal-title":"J. Biol. 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