{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,9]],"date-time":"2025-10-09T19:13:20Z","timestamp":1760037200700,"version":"build-2065373602"},"reference-count":42,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T00:00:00Z","timestamp":1755216000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Jilin Provincial Key Research and Development Program","award":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"],"award-info":[{"award-number":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"]}]},{"name":"National Key Research and Development Program of China","award":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"],"award-info":[{"award-number":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"]}]},{"name":"the 2024 Science and Technology Support Project of the Inner Mongolia Innovation Center of Biological Breeding Technology","award":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"],"award-info":[{"award-number":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"]}]},{"name":"Key R&amp;D Program Project of Ordos City","award":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"],"award-info":[{"award-number":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"]}]},{"name":"Modern Agricultural Industry Technology System of Jilin Province in 2025","award":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"],"award-info":[{"award-number":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"]}]},{"name":"the Modern Agroindustry Technology Research System","award":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"],"award-info":[{"award-number":["20240304155SF","2024YFD1200204-4","2024NSZC01","YF20240036","JLARS-2025-060202","CARS20"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Single-factor and orthogonal experiments were conducted to investigate the biological characteristics of Dichomitus squalens strains isolated from wild fruiting bodies collected in Tekes County, Xinjiang Uygur Autonomous Region. Building upon the optimal mycelial culture conditions identified, domestication cultivation studies were performed, including experiments to induce fruiting body formation. Liquid strains were inoculated into substrates to monitor developmental stages from primordia formation to mature fruiting bodies, with macroscopic characteristics recorded throughout the cultivation process. Crude polysaccharides were extracted from the cultivated fruiting bodies using the water extraction and ethanol precipitation method. The scavenging rates of these polysaccharides against hydroxyl radicals (OH\u2212) and superoxide anion radicals (O2\u2212) were measured to evaluate their in vitro antioxidant activity. Results demonstrated that the optimal growth conditions for D. squalens were as follows: sucrose as the preferred carbon source, yeast extract powder as the optimal nitrogen source, a pH of 5.0, and a temperature of 30 \u00b0C. Among these factors, pH exerted the most significant influence on the mycelial growth rate, followed by nitrogen source, carbon source, and temperature. Mature fruiting bodies developed approximately 57 days after inoculation with liquid strains. The crude polysaccharide extraction yield from the cultivated fruiting bodies reached 7.07%, with a total polysaccharide content of 24.69% in the extract. The crude polysaccharides exhibited potent radical scavenging activity: at a concentration of 5.0 mg\/mL, the hydroxyl radical scavenging rate was 56.74%, while the superoxide anion radical scavenging rate reached 78.3%. These findings indicate that D. squalens possesses significant antioxidant potential.<\/jats:p>","DOI":"10.3390\/jof11080594","type":"journal-article","created":{"date-parts":[[2025,8,15]],"date-time":"2025-08-15T10:33:42Z","timestamp":1755254022000},"page":"594","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Biological Characteristics and Domestication of Dichomitus squalens and the Antioxidant Activity of Its Cultivated Fruiting Bodies"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-4012-1648","authenticated-orcid":false,"given":"Li-Bo","family":"Wang","sequence":"first","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Mycology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0037-9407","authenticated-orcid":false,"given":"Zheng-Xiang","family":"Qi","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Mycology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3939-8852","authenticated-orcid":false,"given":"Tao","family":"Zhang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Mycology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4627-5240","authenticated-orcid":false,"given":"Ke-Qing","family":"Qian","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Mycology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0138-8500","authenticated-orcid":false,"given":"Hai-Yan","family":"Lv","sequence":"additional","affiliation":[{"name":"College of Horticulture, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9508-8188","authenticated-orcid":false,"given":"Bo","family":"Zhang","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Mycology, Jilin Agricultural University, Changchun 130118, China"},{"name":"Industrial Development Institute for Plants, Animals and Fungi Integration of Biyang County, Zhumadian 463799, China"},{"name":"Inner Mongolia Innovation Center of Biological Breeding Technology, Hinggan League Institute of Agricultural and Husbandry Sciences, Ulanhot 137400, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4719-7210","authenticated-orcid":false,"given":"Yu","family":"Li","sequence":"additional","affiliation":[{"name":"Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China"},{"name":"College of Mycology, Jilin Agricultural University, Changchun 130118, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,8,15]]},"reference":[{"unstructured":"Li, Y., Li, T.H., Yang, Z.L., Bau, T., and Dai, Y.C. 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