{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T01:56:51Z","timestamp":1780365411485,"version":"3.54.1"},"reference-count":30,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Interdisciplinary Center for Clinical Research (IZKF) at the University Hospital W\u00fcrzburg","award":["Z-2\/CSP-28"],"award-info":[{"award-number":["Z-2\/CSP-28"]}]},{"name":"Interdisciplinary Center for Clinical Research (IZKF) at the University Hospital W\u00fcrzburg","award":["1369-240"],"award-info":[{"award-number":["1369-240"]}]},{"name":"Robert Koch Institute","award":["Z-2\/CSP-28"],"award-info":[{"award-number":["Z-2\/CSP-28"]}]},{"name":"Robert Koch Institute","award":["1369-240"],"award-info":[{"award-number":["1369-240"]}]},{"name":"German Ministry of Health","award":["Z-2\/CSP-28"],"award-info":[{"award-number":["Z-2\/CSP-28"]}]},{"name":"German Ministry of Health","award":["1369-240"],"award-info":[{"award-number":["1369-240"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Fungal keratitis is a severe infection that often requires surgical intervention and is associated with poor outcomes. Penetrating keratoplasty allows for the complete removal of the fungal infiltrate and thus can be a turning point in therapy. The depth of pathogen invasion, which cannot always be reliably assessed by slit lamp examination, can be accurately determined through histological analysis of the corneal trephinate. In this study, we histologically analyzed two corneal trephinates obtained during an emergency keratoplasty performed for uncontrollable mycotic infections. In case 1, caused by Candida albicans, the infiltrate remained localized at the site of pathogen entry. In contrast, in case 2, Fusarium falciforme demonstrated extensive tissue invasion, spreading destructively throughout the cornea. This invasion pattern suggests that Fusarium keratitis is difficult to control due to its aggressive spreading behavior within the tissue. This explains the high rate of penetrating keratoplasty required in such cases.<\/jats:p>","DOI":"10.3390\/jof11090688","type":"journal-article","created":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T12:08:03Z","timestamp":1758542883000},"page":"688","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Localized Versus Diffuse Corneal Invasion in Fungal Keratitis: Histological Insights from Candida albicans and Fusarium falciforme"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9677-5967","authenticated-orcid":false,"given":"Johanna","family":"Theuersbacher","sequence":"first","affiliation":[{"name":"Department of Ophthalmology, University Hospital W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0008-8714-327X","authenticated-orcid":false,"given":"Lukas","family":"Haug","sequence":"additional","affiliation":[{"name":"Dr. Senckenberg Institute of Pathology, University Hospital Frankfurt, Goethe University Frankfurt, Theodor-Stern-Kai 7, 60590 Frankfurt am Main, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7246-5518","authenticated-orcid":false,"given":"Alexander Maximilian","family":"Aldejohann","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology, University of W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"},{"name":"National Reference Center for Invasive Fungal Infections, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knoell Institute, 07745 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Grit","family":"Walther","sequence":"additional","affiliation":[{"name":"National Reference Center for Invasive Fungal Infections, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knoell Institute, 07745 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Oliver","family":"Kurzai","sequence":"additional","affiliation":[{"name":"Institute for Hygiene and Microbiology, University of W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"},{"name":"National Reference Center for Invasive Fungal Infections, Leibniz Institute for Natural Product Research and Infection Biology-Hans Knoell Institute, 07745 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8613-5875","authenticated-orcid":false,"given":"Daniel","family":"Kampik","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jost","family":"Hillenkamp","sequence":"additional","affiliation":[{"name":"Department of Ophthalmology, University Hospital W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2025,9,22]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"957","DOI":"10.1007\/s00347-019-0871-9","article-title":"The German keratomycosis registry: Initial results of a multicenter survey","volume":"116","author":"Roth","year":"2019","journal-title":"Ophthalmologe"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1079","DOI":"10.1128\/AAC.02456-15","article-title":"International Evaluation of MIC Distributions and Epidemiological Cutoff Value (ECV) Definitions for Fusarium Species Identified by Molecular Methods for the CLSI Broth Microdilution Method","volume":"60","author":"Colombo","year":"2016","journal-title":"Antimicrob. 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