{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T16:59:22Z","timestamp":1765213162337,"version":"3.46.0"},"reference-count":33,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,5]],"date-time":"2025-12-05T00:00:00Z","timestamp":1764892800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003086","name":"Basque Government","doi-asserted-by":"publisher","award":["IT-1657-22"],"award-info":[{"award-number":["IT-1657-22"]}],"id":[{"id":"10.13039\/501100003086","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Candidozyma auris (Candida auris) is an emerging pathogenic yeast of global concern due to its persistence on abiotic and biotic surfaces and the difficulty of treating the severe infections it causes, which are frequently associated with high mortality rates because of its extensive antifungal resistance. Thus, new therapeutic strategies are urgently needed to complement or replace current antifungal drugs. In this study, we evaluated the efficacy of Ca37, a monoclonal antibody (mAb) targeting the alcohol dehydrogenase (Adh) protein of Candida albicans, against C. auris both in vitro and in vivo. Protein electrophoresis and Western Blot analyses demonstrated immunoreactivity of Ca37 mAb with C. auris total protein and cell wall-associated protein extracts, among which Adh was identified. In vitro, incubation with Ca37 mAb significantly reduced the growth of several C. auris strains and enhanced the phagocytic activity of RAW 264.7 murine macrophages. In vivo, Ca37 mAb treatment increased the survival of Galleria mellonella larvae. In a murine model of systemic infection, treated mice displayed improved clinical condition, along with a greater number and larger area of immune-associated foci in the kidneys, suggesting enhanced fungal recognition. These findings support the potential of Ca37 mAb as an antifungal immunotherapy, although further studies in murine models are necessary to establish optimal dosing, efficacy, and mechanisms of action.<\/jats:p>","DOI":"10.3390\/jof11120864","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T16:20:29Z","timestamp":1765210829000},"page":"864","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of the Ca37 Monoclonal Antibody Targeting Alcohol Dehydrogenase Against Candidozyma auris (Candida auris) In Vitro and In Vivo"],"prefix":"10.3390","volume":"11","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5118-4127","authenticated-orcid":false,"given":"Oier","family":"Rodriguez-Erenaga","sequence":"first","affiliation":[{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9707-8733","authenticated-orcid":false,"given":"Maialen","family":"Areitio","sequence":"additional","affiliation":[{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"},{"name":"Section of Immunology, Vetsuisse Faculty, Institute of Experimental Immunology, University of Zurich (UZH), 8057 Zurich, Switzerland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lucia","family":"Abio-Dorronsoro","sequence":"additional","affiliation":[{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nahia","family":"Cazalis-Bereicua","sequence":"additional","affiliation":[{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9994-6602","authenticated-orcid":false,"given":"Leire","family":"Aparicio-Fernandez","sequence":"additional","affiliation":[{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"},{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 01006 Vitoria-Gasteiz, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8540-5629","authenticated-orcid":false,"given":"Leire","family":"Martin-Souto","sequence":"additional","affiliation":[{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4610-3905","authenticated-orcid":false,"given":"Idoia","family":"Buldain","sequence":"additional","affiliation":[{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 01006 Vitoria-Gasteiz, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5528-451X","authenticated-orcid":false,"given":"Be\u00f1at","family":"Zaldibar","sequence":"additional","affiliation":[{"name":"CBET Research Group, Department of Zoology and Animal Cell Biology, Faculty of Science and Technology, Research Centre for Experimental Marine Biology and Biotechnology PIE, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0478-1038","authenticated-orcid":false,"given":"Aitor","family":"Rementeria","sequence":"additional","affiliation":[{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2594-8220","authenticated-orcid":false,"given":"Aitziber","family":"Antoran","sequence":"additional","affiliation":[{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2696-162X","authenticated-orcid":false,"given":"Andoni","family":"Ramirez-Garcia","sequence":"additional","affiliation":[{"name":"MicrobiomicsEHU Research Group, Department of Immunology, Microbiology and Parasitology, University of the Basque Country (UPV\/EHU), 48940 Leioa, Spain"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,5]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1348-0421.2008.00083.x","article-title":"Candida auris Sp. 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