{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,19]],"date-time":"2025-12-19T05:34:17Z","timestamp":1766122457021,"version":"3.48.0"},"reference-count":28,"publisher":"MDPI AG","issue":"12","license":[{"start":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T00:00:00Z","timestamp":1765929600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"NIH","award":["5R01AI146103"],"award-info":[{"award-number":["5R01AI146103"]}]},{"DOI":"10.13039\/100007699","name":"University of Georgia","doi-asserted-by":"crossref","id":[{"id":"10.13039\/100007699","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The fungal pathogen Candida albicans can infect diverse tissues, a reflection of its broad metabolic repertoire. The transcription factor Adr1 is required for utilization of several citric acid cycle intermediates that are found in tissue. Many Adr1-activated genes encode enzymes with well-defined roles in citrate metabolism or gluconeogenesis. Here, we focus on HGT17 (C4_01070W, orf19.4682), an Adr1-activated gene that encodes a possible citrate transporter. We provide two lines of evidence that HGT17 is a key functional target of Adr1. First, forced expression of HGT17 in an adr1\u0394\/\u0394 mutant improves growth on citrate as a carbon source. Second, hgt17\u0394\/\u0394 and adr1\u0394\/\u0394 mutants incubated in citrate medium present similar gene expression defects compared to the wild type. Noteworthy is down-regulation in both mutants of citric acid cycle genes, glycolysis\/gluconeogenesis genes, and ergosterol synthesis genes. These common features may reflect a specific effect of citrate as an inducer of citric acid cycle enzymes or a global effect of carbon and energy limitation. In either case, the results argue that reduced HGT17 expression contributes substantially to the impact of an adr1\u0394\/\u0394 mutation on growth and gene expression.<\/jats:p>","DOI":"10.3390\/jof11120889","type":"journal-article","created":{"date-parts":[[2025,12,17]],"date-time":"2025-12-17T10:41:23Z","timestamp":1765968083000},"page":"889","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Hgt17-Adr1 Relationship in Candida albicans Citrate Utilization"],"prefix":"10.3390","volume":"11","author":[{"given":"Amelia M.","family":"White","sequence":"first","affiliation":[{"name":"Department of Microbiology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0868-4000","authenticated-orcid":false,"given":"Aaron P.","family":"Mitchell","sequence":"additional","affiliation":[{"name":"Department of Microbiology, University of Georgia, Athens, GA 30602, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2025,12,17]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"432","DOI":"10.1016\/j.chom.2019.02.008","article-title":"Candida albicans Morphogenesis Programs Control the Balance between Gut Commensalism and Invasive Infection","volume":"25","author":"Witchley","year":"2019","journal-title":"Cell Host Microbe"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"614","DOI":"10.1016\/j.tim.2014.07.001","article-title":"Metabolism impacts upon Candida immunogenicity and pathogenicity at multiple levels","volume":"22","author":"Brown","year":"2014","journal-title":"Trends Microbiol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"165rv113","DOI":"10.1126\/scitranslmed.3004404","article-title":"Hidden killers: Human fungal infections","volume":"4","author":"Brown","year":"2012","journal-title":"Sci. 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