{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,31]],"date-time":"2026-01-31T01:00:20Z","timestamp":1769821220326,"version":"3.49.0"},"reference-count":67,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T00:00:00Z","timestamp":1769731200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Department of Biotechnology (DBT), Government of India","award":["BT\/PR47834\/MED\/29\/1656\/2023"],"award-info":[{"award-number":["BT\/PR47834\/MED\/29\/1656\/2023"]}]},{"name":"Boost to University Interdisciplinary Life Science Departments","award":["BT\/INF\/22\/SP25072\/2022"],"award-info":[{"award-number":["BT\/INF\/22\/SP25072\/2022"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>In Candida species, including Candidozyma auris (formerly Candida auris), overexpression of efflux pumps is a well-established mechanism of antifungal resistance. However, accumulating evidence indicates that impaired drug import may also significantly contribute to reduced antifungal susceptibility. Sugar importers, historically viewed solely as hexose transporters (HGTs), are now emerging as potential indirect modulators of antifungal uptake. Here, we performed a comprehensive inventory and functional analysis of the HGT family in C. auris to assess its contribution to antifungal import. Phylogenetic analyses revealed that C. auris HGTs are more closely related to those of Candida albicans (C. albicans) than Saccharomyces cerevisiae (S. cerevisiae). All HGT genes showed basal expression, with several significantly downregulated upon fluconazole (FLC) exposure. To establish functional relevance, we generated a mini-library of HGT deletion mutants. Notably, the \u0394hgt13 strain exhibited markedly increased FLC resistance, concomitant with reduced intracellular FLC accumulation and decreased membrane permeability. Consistently, molecular docking and molecular dynamics simulations demonstrated strong and stable interactions between FLC and Hgt13p. Together, these findings implicate Hgt13p as a key determinant of FLC import and membrane permeability, revealing reduced FLC import could also contribute to antifungal resistance in C. auris.<\/jats:p>","DOI":"10.3390\/jof12020094","type":"journal-article","created":{"date-parts":[[2026,1,30]],"date-time":"2026-01-30T13:04:46Z","timestamp":1769778286000},"page":"94","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comprehensive Insights into Sugar Transporters of Candidozyma auris and Their Roles in Antifungal Resistance"],"prefix":"10.3390","volume":"12","author":[{"given":"Praveen","family":"Kumar","sequence":"first","affiliation":[{"name":"Amity Institute of Biotechnology, Amity University Gurgaon, Manesar 122413, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mohit","family":"Kumar","sequence":"additional","affiliation":[{"name":"Amity Institute of Biotechnology, Amity University Gurgaon, Manesar 122413, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amandeep","family":"Saini","sequence":"additional","affiliation":[{"name":"Amity Institute of Biotechnology, Amity University Gurgaon, Manesar 122413, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sheikh Owais","family":"Mohamad","sequence":"additional","affiliation":[{"name":"Department of Bioresources, School of Biological Science, University of Kashmir, Srinagar 190006, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-8266-8605","authenticated-orcid":false,"given":"Basharat","family":"Ali","sequence":"additional","affiliation":[{"name":"School of Life Sciences, Jawaharlal Nehru University, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7328-4057","authenticated-orcid":false,"given":"Brooke D.","family":"Esquivel","sequence":"additional","affiliation":[{"name":"School of Science and Engineering, University of Missouri Kansas City, Kansas City, MO 64110, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9319-4593","authenticated-orcid":false,"given":"Atanu","family":"Banerjee","sequence":"additional","affiliation":[{"name":"Amity Institute of Biotechnology, Amity University Gurgaon, Manesar 122413, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3229-1378","authenticated-orcid":false,"given":"Theodore C.","family":"White","sequence":"additional","affiliation":[{"name":"School of Science and Engineering, University of Missouri Kansas City, Kansas City, MO 64110, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naseem A.","family":"Gaur","sequence":"additional","affiliation":[{"name":"Yeast Biofuel Group, International Centre for Genetic Engineering and Biotechnology, New Delhi 110067, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2242-0551","authenticated-orcid":false,"given":"Abdul Haseeb","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Bioresources, School of Biological Science, University of Kashmir, Srinagar 190006, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amresh","family":"Prakash","sequence":"additional","affiliation":[{"name":"Amity Institute of Biotechnology, Amity University Gurgaon, Manesar 122413, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rajendra","family":"Prasad","sequence":"additional","affiliation":[{"name":"Amity Institute of Biotechnology, Amity University Gurgaon, Manesar 122413, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,1,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1007\/s12275-016-0647-8","article-title":"Human Fungal Pathogens: Why Should We Learn?","volume":"54","author":"Kim","year":"2016","journal-title":"J. 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