{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T02:59:00Z","timestamp":1770173940171,"version":"3.49.0"},"reference-count":26,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T00:00:00Z","timestamp":1770076800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32271898"],"award-info":[{"award-number":["32271898"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Esteya vermicola is a nematophagous fungus with strong parasitic ability against the pinewood nematode (Bursaphelenchus xylophilus) and shows great potential for the biological control of pine wilt disease. However, this fungus is highly sensitive to environmental stress factors and often exhibits early necrosis when cultured on conventional nutrient-rich media, limiting its large-scale application. In this study, we optimized the long-term cultivation and conidiation conditions of E. vermicola CBS115803 by supplementing minimal medium (MM) with amino acids, and evaluated its stress tolerance and infectivity against the pinewood nematode. Among 20 tested amino acids, histidine significantly increased total conidia production, while arginine, glutamine, and proline markedly promoted the formation of lunate conidia. The combination of arginine, histidine, glutamine, and proline (AHGP) produced the highest overall conidia yield and lunate conidia proportion. The MM + AHGP medium maintained long-term colony viability, whereas colonies on PDA and CM media showed obvious degeneration. This formulation also improved mycelial growth, total conidiation, and the proportion of lunate conidia. Moreover, conidia produced on MM + AHGP exhibited the highest germination rates and infectivity under various stress conditions, including cold, heat, oxidative, osmotic, and UV stresses. Conidia germination was significantly enhanced following treatment at 0 \u00b0C, suggesting that low temperatures may activate dormancy-breaking pathways. This amino acid-optimized medium offers an effective technical foundation for stable large-scale production and storage of E. vermicola conidia, providing a new avenue for the biocontrol of pine wilt disease.<\/jats:p>","DOI":"10.3390\/jof12020107","type":"journal-article","created":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T12:46:11Z","timestamp":1770122771000},"page":"107","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Amino Acid-Optimized Medium Improves Long-Term Growth, Conidiation, Stress Tolerance, and Biocontrol Performance of Esteya vermicola Against Pinewood Nematode"],"prefix":"10.3390","volume":"12","author":[{"given":"Xuan","family":"Peng","sequence":"first","affiliation":[{"name":"The College of Life Science, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yilan","family":"Wang","sequence":"additional","affiliation":[{"name":"The College of Life Science, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhongwu","family":"Cheng","sequence":"additional","affiliation":[{"name":"The College of Life Science, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tengqing","family":"Ye","sequence":"additional","affiliation":[{"name":"The College of Life Science, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lihui","family":"Han","sequence":"additional","affiliation":[{"name":"The College of Life Science, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ping","family":"Lin","sequence":"additional","affiliation":[{"name":"The College of Life Science, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Li","sequence":"additional","affiliation":[{"name":"The College of Life Science, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7835-3266","authenticated-orcid":false,"given":"Chengjian","family":"Xie","sequence":"additional","affiliation":[{"name":"The College of Life Science, Chongqing Normal University, Chongqing 401331, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"483","DOI":"10.1111\/jen.12604","article-title":"Transmission of Bursaphelenchus xylophilus between Monochamus alternatus and Monochamus saltuarius through Interspecific Mating Behaviour","volume":"143","author":"Togashi","year":"2019","journal-title":"J. Appl. Entomol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1","DOI":"10.48044\/jauf.1982.001","article-title":"Pine Wilt-A Disease You Should Know","volume":"8","author":"Dropkin","year":"1982","journal-title":"Arboric. Urban For."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Cardoso, J.M.S., Manadas, B., Abrantes, I., Robertson, L., Arcos, S.C., Troya, M.T., Navas, A., and Fonseca, L. (2024). Pine Wilt Disease: What Do We Know from Proteomics?. BMC Plant Biol., 24.","DOI":"10.1186\/s12870-024-04771-9"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Li, M., Li, H., Ding, X., Wang, L., Wang, X., and Chen, F. (2022). The Detection of Pine Wilt Disease: A Literature Review. Int. J. Mol. Sci., 23.","DOI":"10.3390\/ijms231810797"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"1026","DOI":"10.1111\/ppa.13875","article-title":"Pine Wilt Disease: A Global Threat to Forestry","volume":"73","author":"Back","year":"2024","journal-title":"Plant Pathol."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Faria, J.M.S., Barbosa, P., Vieira, P., Vicente, C.S.L., Figueiredo, A.C., and Mota, M. (2021). Phytochemicals as Biopesticides Against the Pinewood Nematode Bursaphelenchus Xylophilus: A Review on Essential Oils and Their Volatiles. Plants, 10.","DOI":"10.3390\/plants10122614"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Fan, Y., Liu, L., Wu, C., Yu, G., Wang, Z., Fan, J., and Tu, C. (2023). The Effect of Regulating Soil pH on the Control of Pine Wilt Disease in a Black Pine Forest. Forests, 14.","DOI":"10.3390\/f14081583"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"380","DOI":"10.1007\/s12275-007-0122-7","article-title":"High Infectivity of an Endoparasitic Fungus Strain, Esteya Vermicola, Against Nematodes","volume":"46","author":"Wang","year":"2008","journal-title":"J. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Pires, D., Vicente, C.S.L., Inacio, M.L., and Mota, M. (2022). The Potential of Esteya spp. for the Biocontrol of the Pinewood Nematode, Bursaphelenchus xylophilus. Microorganisms, 10.","DOI":"10.3390\/microorganisms10010168"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1002\/ps.7809","article-title":"The Essential Role of Arginine Biosynthetic Genes in Lunate Conidia Formation, Conidiation, Mycelial Growth, and Virulence of Nematophagous Fungus, Esteya vermicola CBS115803","volume":"80","author":"Chen","year":"2024","journal-title":"Pest Manag. Sci."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1111\/jam.14964","article-title":"Effect of Culture Conditions on Conidia Production and Enhancement of Environmental Stress Resistance of Esteya Vermicola in Solid-State Fermentation","volume":"131","author":"Zhu","year":"2021","journal-title":"J. Appl. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"86","DOI":"10.21307\/jofnem-2017-048","article-title":"Esteya Vermicola Controls the Pinewood Nematode, Bursaphelenchus Xylophilus, in Pine Seedlings","volume":"49","author":"Wang","year":"2017","journal-title":"J. Nematol."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"2854","DOI":"10.1002\/ps.5839","article-title":"In Vivo Infection of Bursaphelenchus Xylophilus by the Fungus Esteya vermicola","volume":"76","author":"Wang","year":"2020","journal-title":"Pest Manag. Sci."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Lin, F., Ye, J., Wang, H., Zhang, A., and Zhao, B. (2013). Host Deception: Predaceous Fungus, Esteya vermicola, Entices Pine Wood Nematode by Mimicking the Scent of Pine Tree for Nutrient. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0071676"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"201","DOI":"10.1264\/jsme2.ME16167","article-title":"Esteya Vermicola, a Nematophagous Fungus Attacking the Pine Wood Nematode, Harbors a Bacterial Endosymbiont Affiliated with Gammaproteobacteria","volume":"32","author":"Wang","year":"2017","journal-title":"Microbes Environ."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"838","DOI":"10.1139\/w11-068","article-title":"Growth of Esteya Vermicola in Media Amended with Nitrogen Sources Yields Conidia with Increased Predacity and Resistance to Environmental Stress","volume":"57","author":"Wang","year":"2011","journal-title":"Can. J. Microbiol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"306","DOI":"10.1007\/s00284-013-0365-y","article-title":"Effect of Nutrition and Environmental Factors on the Endoparasitic Fungus Esteya vermicola, a Biocontrol Agent Against Pine Wilt Disease","volume":"67","author":"Xue","year":"2013","journal-title":"Curr. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2805","DOI":"10.1007\/s11274-014-1704-2","article-title":"Optimization of storage condition for maintaining long-term viability of nematophagous fungus Esteya vermicola as biocontrol agent against pinewood nematode","volume":"30","author":"Xue","year":"2014","journal-title":"World J. Microbiol. Biotechnol."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Mohamed, H., Awad, M.F., Shah, A.M., Nazir, Y., Naz, T., Hassane, A., Nosheen, S., and Song, Y. (2022). Evaluation of Different Standard Amino Acids to Enhance the Biomass, Lipid, Fatty Acid, and \u03b3-Linolenic Acid Production in Rhizomucor pusillus and Mucor circinelloides. Front. Nutr., 9.","DOI":"10.3389\/fnut.2022.876817"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"105639","DOI":"10.1016\/j.biocontrol.2024.105639","article-title":"Amino acids promote the rejuvenation of degenerated Metarhizium anisopliae","volume":"198","author":"Yang","year":"2024","journal-title":"Biol. Control"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Ar\u00e9valo-Jaimes, B.V., and Torrents, E. (2024). Died or Not Dyed: Assessment of Viability and Vitality Dyes on Planktonic Cells and Biofilms from Candida parapsilosis. J. Fungi, 10.","DOI":"10.3390\/jof10030209"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1080\/07388550290789432","article-title":"The Autolysis of Industrial Filamentous Fungi","volume":"22","author":"White","year":"2002","journal-title":"Crit. Rev. Biotechnol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"2353","DOI":"10.1139\/b91-296","article-title":"The Influence of Cysteine, Cysteine Analogs, and Other Amino Acids on Spore Germination of Alternaria Species","volume":"69","author":"Daigle","year":"1991","journal-title":"Can. J. Bot."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Liu, X., Ye, G., Feng, Z., Zhou, Y., Qin, Y., Yao, Q., and Zhu, H. (2024). Cold Storage Promotes Germination and Colonization of Arbuscular Mycorrhizal Fungal Hyphae as Propagules. Front. Plant Sci., 15.","DOI":"10.3389\/fpls.2024.1450829"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"2080","DOI":"10.1094\/PDIS-02-16-0215-RE","article-title":"Effects of Temperature and Duration of Preconditioning Cold Treatment on Sclerotial Germination of Claviceps purpurea","volume":"100","author":"Uppala","year":"2016","journal-title":"Plant Dis."},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Deng, Y., Wang, R., Zhang, Y., Li, J., and Gooneratne, R. (2023). Effect of Amino Acids on Fusarium oxysporum Growth and Pathogenicity Regulated by TORC1-Tap42 Gene and Related Interaction Protein Analysis. Foods, 12.","DOI":"10.3390\/foods12091829"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/2\/107\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,3]],"date-time":"2026-02-03T13:10:51Z","timestamp":1770124251000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/2\/107"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,3]]},"references-count":26,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["jof12020107"],"URL":"https:\/\/doi.org\/10.3390\/jof12020107","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,3]]}}}