{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T13:00:16Z","timestamp":1770814816832,"version":"3.50.1"},"reference-count":40,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T00:00:00Z","timestamp":1770768000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["NKFIH K 147510"],"award-info":[{"award-number":["NKFIH K 147510"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["HUN-REN 2001007"],"award-info":[{"award-number":["HUN-REN 2001007"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100018818","name":"National Research, Development and Innovation Office","doi-asserted-by":"publisher","award":["TM NKFIA TKP2021-EGA-28"],"award-info":[{"award-number":["TM NKFIA TKP2021-EGA-28"]}],"id":[{"id":"10.13039\/501100018818","id-type":"DOI","asserted-by":"publisher"}]},{"name":"University of Szeged Open Access Fund","award":["8132"],"award-info":[{"award-number":["8132"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The species Candidozyma auris (formerly known as Candida auris) can be subdivided into four major and two minor clades. It is considered an emerging multidrug-resistant pathogen that causes invasive outbreaks around the world. Therefore, the accurate identification of this species plays an important role in combating invasion and facilitating pathogenic management. In our study an optional identification method was developed considering the possibility of using cellular fatty acids (FAs) as a taxonomic and diagnostic tool. FAs were recorded in the collected C. auris strains, and the species characteristic components were determined. Within the isolates examined, the clades were also separated in the statistical analysis. Furthermore, FAs from strains belonging to clade I and II have been divided into two distinct clusters. In testing the performance of the method, all identified samples showed good matches with the established C. auris record in the database without misreading. Taken together, cellular fatty acids were investigated as potential discriminatory biomarkers. The results suggest that this approach can distinguish C. auris from related species and provides distinctive fatty acid profiles for the investigated C. auris clades. The present findings revealed the first report on the application of whole cell FA components as taxonomic features in C. auris.<\/jats:p>","DOI":"10.3390\/jof12020130","type":"journal-article","created":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T09:15:12Z","timestamp":1770801312000},"page":"130","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fatty Acid Profiling Identification Method of Emerging Fungal Pathogen Candidozyma auris (Formally Candida auris)"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6042-3353","authenticated-orcid":false,"given":"Thu","family":"Huynh","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p Fasor 52, H-6726 Szeged, Hungary"},{"name":"Department of Biotechnology, Faculty of Chemical Engineering, Ho Chi Minh University of Technology (HCMUT), 268 Ly Thuong Kiet Street, District 10, Ho Chi Minh City 72607, Vietnam"},{"name":"Vietnam National University Ho Chi Minh City, Linh Trung Ward, Thu Duc District, Ho Chi Minh City 71351, Vietnam"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0232-635X","authenticated-orcid":false,"given":"Flora","family":"Bohner","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p Fasor 52, H-6726 Szeged, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Adiyadolgor","family":"Turbat","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p Fasor 52, H-6726 Szeged, Hungary"},{"name":"Laboratory of Microbiology, Institute of General and Experimental Biology, Mongolian Academy of Science, Ulaanbaatar 13330, Mongolia"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6666-1384","authenticated-orcid":false,"given":"Gy\u00f6rgy","family":"Sipos","sequence":"additional","affiliation":[{"name":"Functional Genomics and Bioinformatics Group, Faculty of Forestry, University of Sopron, Bajcsy-Zsilinszky Str. 4., H-9400 Sopron, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2939-9580","authenticated-orcid":false,"given":"Attila","family":"G\u00e1cser","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p Fasor 52, H-6726 Szeged, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0009-7773","authenticated-orcid":false,"given":"Csaba","family":"V\u00e1gv\u00f6lgyi","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p Fasor 52, H-6726 Szeged, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8211-5431","authenticated-orcid":false,"given":"Tam\u00e1s","family":"Papp","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p Fasor 52, H-6726 Szeged, Hungary"},{"name":"HUN-REN-SZTE Pathomechanisms of Fungal Infections Research Group, University of Szeged, K\u00f6z\u00e9p Fasor 52, H-6726 Szeged, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M\u00f3nika","family":"Varga","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p Fasor 52, H-6726 Szeged, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1651-4623","authenticated-orcid":false,"given":"Andr\u00e1s","family":"Szekeres","sequence":"additional","affiliation":[{"name":"Department of Biotechnology and Microbiology, Faculty of Science and Informatics, University of Szeged, K\u00f6z\u00e9p Fasor 52, H-6726 Szeged, Hungary"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2026,2,11]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"41","DOI":"10.1111\/j.1348-0421.2008.00083.x","article-title":"Candida auris sp. nov., a novel ascomycetous yeast isolated from the external ear canal of an inpatient in a Japanese hospital","volume":"53","author":"Satoh","year":"2009","journal-title":"Microbiol. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2405","DOI":"10.1080\/22221751.2022.2125349","article-title":"Confirmation of fifth Candida auris clade by whole genome sequencing","volume":"11","author":"Spruijtenburg","year":"2022","journal-title":"Emerg. Microbes Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1111\/myc.12886","article-title":"Candida auris otomycosis in Iran and review of recent literature","volume":"62","author":"Abastabar","year":"2019","journal-title":"Mycoses"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"100878","DOI":"10.1016\/S2666-5247(24)00101-0","article-title":"Detection and characterisation of a sixth Candida auris clade in Singapore: A genomic and phenotypic study","volume":"5","author":"Suphavilai","year":"2024","journal-title":"Lancet Microbe"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"743","DOI":"10.1016\/j.jiac.2019.05.034","article-title":"Candida auris: A pathogen difficult to identify, treat, and eradicate and its characteristics in Japanese strains","volume":"25","author":"Iguchi","year":"2019","journal-title":"J. Infect. Chemother."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Kordalewska, M., and Perlin, D.S. (2019). Identification of drug resistant Candida auris. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.01918"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e280","DOI":"10.1016\/S1473-3099(24)00749-7","article-title":"Global guideline for the diagnosis and management of candidiasis: An initiative of the ECMM in cooperation with ISHAM and ASM","volume":"25","author":"Cornely","year":"2025","journal-title":"Lancet Infect Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"5346","DOI":"10.1038\/s41467-018-07779-6","article-title":"Genomic insights into multidrug-resistance, mating and virulence in Candida auris and related emerging species","volume":"9","author":"Gade","year":"2018","journal-title":"Nat. Commun."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1146\/annurev-med-061523-021233","article-title":"Candida auris: Epidemiology and antifungal strategy","volume":"76","author":"Eix","year":"2025","journal-title":"Annu. Rev. Med."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1287","DOI":"10.2147\/IDR.S249864","article-title":"Candida auris: From multidrug resistance to pan-resistant strains","volume":"13","author":"Ademe","year":"2020","journal-title":"Infect. Drug Resist."},{"key":"ref_11","unstructured":"Centers for Disease Control and Prevention (2025, October 10). Antifungal Susceptibility Testing for C. auris, Available online: https:\/\/www.cdc.gov\/candida-auris\/hcp\/laboratories\/antifungal-susceptibility-testing.html."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Caceres, D.H., Forsberg, K., Welsh, R.M., Sexton, D.J., Lockhart, S.R., Jackson, B.R., and Chiller, T. (2019). Candida auris: A review of recommendations for detection and control in healthcare settings. J. Fungi, 5.","DOI":"10.3390\/jof5040111"},{"key":"ref_13","unstructured":"Centers for Disease Control and Prevention (2025, October 10). Identification of C. auris, Available online: https:\/\/www.cdc.gov\/candida-auris\/hcp\/laboratories\/identification-of-c-auris.html."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"116","DOI":"10.1007\/s12281-021-00420-y","article-title":"Candida auris: Diagnostic challenges and emerging opportunities for the clinical microbiology laboratory","volume":"15","author":"Keighley","year":"2021","journal-title":"Curr. Fungal Infect. Rep."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1823","DOI":"10.1128\/JCM.00367-15","article-title":"Multidrug-resistant Candida auris misidentified as Candida haemulonii: Characterization by matrix-assisted laser desorption ionization-time of flight mass spectrometry and DNA sequencing and its antifungal susceptibility profile variability by Vitek 2, CLSI broth microdilution, and Etest method","volume":"53","author":"Kathuria","year":"2015","journal-title":"J. Clin. Microbiol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2206","DOI":"10.4014\/jmb.1609.09012","article-title":"Identification of uncommon Candida species using commercial identification system","volume":"26","author":"Kim","year":"2016","journal-title":"J. Microbiol. Biotechnol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"e0080821","DOI":"10.1128\/jcm.00808-21","article-title":"Tools for detecting a \u201csuperbug\u201d: Updates on Candida auris testing","volume":"60","author":"Lockhart","year":"2022","journal-title":"J. Clin. Microbiol."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"807","DOI":"10.1007\/s10096-016-2864-9","article-title":"Matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF MS) for rapid identification of micro-organisms in the routine clinical microbiology laboratory","volume":"36","author":"Wattal","year":"2017","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"564","DOI":"10.1002\/jobm.200900084","article-title":"Isolation and identification of a Candida digboiensis strain from an extreme acid mine drainage of the Lignite Mine, Gujarat","volume":"49","author":"Patel","year":"2009","journal-title":"J. Basic Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"213","DOI":"10.1016\/S0732-8893(00)00205-4","article-title":"Discriminative power of fatty acid methyl ester (FAME) analysis using the Microbial Identification System (MIS) for Candida (Torulopsis) glabrata and Saccharomyces cerevisiae","volume":"38","author":"Schmidt","year":"2000","journal-title":"Diag. Microbiol. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"3696","DOI":"10.1128\/JCM.38.10.3696-3704.2000","article-title":"Differentiation between Candida dubliniensis and Candida albicans by fatty acid methyl ester analysis using gas-liquid chromatography","volume":"38","author":"Schmidt","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_22","unstructured":"MIDI Inc. (2018). The Sherlock Chromatographic Analysis System Operating Manual, MIDI Inc.. [6th ed.]."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"134","DOI":"10.1093\/cid\/ciw691","article-title":"Simultaneous emergence of multidrug-resistant Candida auris on 3 continents confirmed by whole-genome sequencing and epidemiological analyses","volume":"64","author":"Lockhart","year":"2017","journal-title":"Clin. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Sekizuka, T., Iguchi, S., Umeyama, T., Inamine, Y., Makimura, K., Kuroda, M., Miyazaki, Y., and Kikuchi, K. (2019). Clade II Candida auris possess genomic structural variations related to an ancestral strain. PLoS ONE, 14.","DOI":"10.1371\/journal.pone.0223433"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.cmi.2015.10.022","article-title":"Evidence of genotypic diversity among Candida auris isolates by multilocus sequence typing, matrix-assisted laser desorption ionization time-of-flight mass spectrometry and amplified fragment length polymorphism","volume":"22","author":"Prakash","year":"2016","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"e02971-19","DOI":"10.1128\/mBio.02971-19","article-title":"Development of Candida auris short tandem repeat typing and its application to a global collection of isolates","volume":"11","author":"Puts","year":"2020","journal-title":"mBio"},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Carolus, H., Jacobs, S., Romero, C.L., Deparis, Q., Cuomo, C.A., Meis, J.F., and Van Dijck, P. (2021). Diagnostic allele\u2013specific PCR for the identification of Candida auris clades. J. Fungi, 7.","DOI":"10.3390\/jof7090754"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e0063422","DOI":"10.1128\/spectrum.00634-22","article-title":"ClaID: A Rapid Method of clade-level identification of the multidrug resistant human fungal pathogen Candida auris","volume":"10","author":"Narayanan","year":"2022","journal-title":"Microbiol. Spectr."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e01444-24","DOI":"10.1128\/spectrum.01444-24","article-title":"Improvement of a MALDI-TOF database for the reliable identification of Candidozyma auris (formally Candida auris) and related species","volume":"13","author":"Zhu","year":"2025","journal-title":"Microbiol. Spectr."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Grenfell, R.C., da Silva Junior, A.R., Del Negro, G.M.B., Munhoz, R.B., Gimenes, V.M.F., Assis, D.M., Rockstroh, A.C., Motta, A.L., Rossi, F., and Juliano, L. (2016). Identification of Candida haemulonii complex species: Use of ClinProTools to overcome limitations of the Bruker Biotyper, VITEK MSTM IVD, and VITEK MS RUO databases. Front. Microbiol., 7.","DOI":"10.3389\/fmicb.2016.00940"},{"key":"ref_31","first-page":"e00029-17","article-title":"Candida auris Incident Management Team; Manuel R, Brown CS. Candida auris: A review of the literature","volume":"31","author":"Taori","year":"2018","journal-title":"Clin. Microbiol. Rev."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"513","DOI":"10.1111\/myc.12907","article-title":"A high-throughput and rapid method for accurate identification of emerging multidrug-resistant Candida auris","volume":"62","author":"Ahmad","year":"2019","journal-title":"Mycoses"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"e01223-17","DOI":"10.1128\/JCM.01223-17","article-title":"Development and validation of a real-time PCR assay for rapid detection of Candida auris from surveillance samples","volume":"56","author":"Leach","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_34","first-page":"001478","article-title":"Genomic analysis of Candida auris transmission within an urban region","volume":"11","author":"Barbian","year":"2025","journal-title":"Microb. Genom."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"107","DOI":"10.5858\/arpa.2018-0508-RA","article-title":"Candida auris: An emerging yeast pathogen posing distinct challenges for laboratory diagnostics, treatment, and infection prevention","volume":"144","author":"Hata","year":"2019","journal-title":"Arch. Pathol. Labor. Med."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"e00591-18","DOI":"10.1128\/JCM.00591-18","article-title":"Rapid detection of Candida auris based on Loop-Mediated Isothermal Amplification (LAMP)","volume":"56","author":"Yamamoto","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1007\/s11046-024-00892-9","article-title":"Assessment of LAMPAuris for rapid detection of Candida auris in clinical specimens","volume":"189","author":"Yamamoto","year":"2024","journal-title":"Mycopathologia"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"e00257-19","DOI":"10.1128\/mSystems.00257-19","article-title":"Multi-omics signature of Candida auris, an emerging and multidrug-resistant pathogen","volume":"4","author":"Heyman","year":"2019","journal-title":"MSystems"},{"key":"ref_39","first-page":"e00822-21","article-title":"Comparative molecular and immunoregulatory analysis of extracellular vesicles from Candida albicans and Candida auris","volume":"6","author":"Heyman","year":"2021","journal-title":"MSystems"},{"key":"ref_40","doi-asserted-by":"crossref","unstructured":"Valdez, A.F., Bohner, F., Goldman, J.P., Jaquiery, A.B., Amaral, E.V.C.d., Correa-Junior, D., Draganski, A., Nimrichter, L., Nosanchuk, J.D., and Zamith-Miranda, D. (2025). Candida auris antifungal resistance, virulence and susceptibility to a novel nitric oxide-releasing microparticle and its correlations to clade identification. Microbiol. Res., 16.","DOI":"10.3390\/microbiolres16010015"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/2\/130\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,2,11]],"date-time":"2026-02-11T10:08:36Z","timestamp":1770804516000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/2\/130"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,2,11]]},"references-count":40,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2026,2]]}},"alternative-id":["jof12020130"],"URL":"https:\/\/doi.org\/10.3390\/jof12020130","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,2,11]]}}}