{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T17:02:55Z","timestamp":1780938175360,"version":"3.54.1"},"reference-count":47,"publisher":"MDPI AG","issue":"6","license":[{"start":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T00:00:00Z","timestamp":1780876800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["FGEU-2025-0005"],"award-info":[{"award-number":["FGEU-2025-0005"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012190","name":"Ministry of Science and Higher Education of the Russian Federation","doi-asserted-by":"publisher","award":["125031003376-3"],"award-info":[{"award-number":["125031003376-3"]}],"id":[{"id":"10.13039\/501100012190","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>In this study, four Fusarium strains isolated from Poaceae plants infected by Claviceps spp. and one strain isolated from the stem of Cirsium arvense collected from two regions of Russia that are separated by a long distance were analyzed in detail. These fungi were accurately identified through a phylogenetic analysis of the fragments of translation elongation factor 1-\u03b1 and RNA polymerase second largest subunit loci. Four of them belong to F. heterosporum species, and one strain, MFG 13060, together with the historical reference strain BBA 62226, forms a distinct lineage within the F. heterosporum species complex (FHSC). The morphological features of the anamorph structures of the fungi within the FHSC are presented. All the analyzed F. heterosporum strains are heterothallic and require a partner to mate. The fertile perithecia of F. heterosporum were obtained in a crossing experiment, and the teleomorph structures were characterized in detail. The screening of 19 mycotoxins typically produced by Fusarium fungi using high-performance liquid chromatography with tandem mass spectrometry revealed the ability of the strains to produce only moniliformin on an autoclaved rice substrate. A reassessment of the species diversity, distribution, and significance of fungi belonging to the FHSC is necessary to elucidate the unclear relationships between F. heterosporum, Claviceps fungi, and cereal plants.<\/jats:p>","DOI":"10.3390\/jof12060416","type":"journal-article","created":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T16:25:16Z","timestamp":1780935916000},"page":"416","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Insights into the Species Diversity and Features of Fungi in the Fusarium heterosporum Species Complex"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-5350-3221","authenticated-orcid":false,"given":"Olga P.","family":"Gavrilova","sequence":"first","affiliation":[{"name":"Laboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection, 196608 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7657-6618","authenticated-orcid":false,"given":"Aleksandra S.","family":"Orina","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection, 196608 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadezhda N.","family":"Gogina","sequence":"additional","affiliation":[{"name":"Laboratory of Biochemical Analysis, All-Russian Scientific Research and Technological Institute of Poultry, 141311 Sergiev Posad, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3276-561X","authenticated-orcid":false,"given":"Tatiana Yu.","family":"Gagkaeva","sequence":"additional","affiliation":[{"name":"Laboratory of Mycology and Phytopathology, All-Russian Institute of Plant Protection, 196608 St. Petersburg, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"2092","DOI":"10.5943\/mycosphere\/14\/1\/25","article-title":"Endophytic Fusarium and allied fungi from Rosa roxburghii in China","volume":"14","author":"Zhang","year":"2023","journal-title":"Mycosphere"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Fan, Q., Su, P., Xiao, J., Lou, F., Huang, X., Yang, Z., Chen, B., Shen, P., and Wang, Y. (2025). Phylogenomic, morphological, and phylogenetic evidence reveals five new species and two new host records of Nectriaceae (Hypocreales) from China. Biology, 14.","DOI":"10.3390\/biology14070871"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Gerlach, W., and Nirenberg, H. (1982). The Genus Fusarium: A Pictorial Atlas, Kommissionsverlag P. Parey.","DOI":"10.2307\/3792677"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"100116","DOI":"10.1016\/j.simyco.2021.100116","article-title":"Fusarium: More than a node or a foot-shaped basal cell","volume":"98","author":"Crous","year":"2021","journal-title":"Stud. Mycol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"13","DOI":"10.5598\/imafungus.2018.09.01.02","article-title":"Karyotype evolution in Fusarium","volume":"9","author":"Waalwijk","year":"2018","journal-title":"IMA Fungus"},{"key":"ref_6","unstructured":"Booth, C. (1971). The Genus Fusarium, Commonwealth Mycological Institute."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"120","DOI":"10.1071\/AP96020","article-title":"Fusarium heterosporum associated with paspalum ergot in eastern Australia","volume":"25","author":"Ali","year":"1996","journal-title":"Australas. Plant Pathol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Leslie, J.F., and Summerell, B.A. (2006). The Fusarium Laboratory Manual, Blackwell Publishing.","DOI":"10.1002\/9780470278376"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"293","DOI":"10.1016\/j.funbio.2010.01.005","article-title":"Characterisation of New Zealand Fusarium populations using a polyphasic approach differentiates the F. avenaceum\/F. acuminatum\/F. tricinctum species complex in cereal and grassland systems","volume":"114","author":"Harrow","year":"2010","journal-title":"Fungal Biol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"ycaf124","DOI":"10.1093\/ismeco\/ycaf124","article-title":"Synthetic spike-in metabarcoding for plant pathogen diagnostics results in precise quantification of copy number within the genus Fusarium","volume":"5","author":"Oppenheimer","year":"2025","journal-title":"ISME Commun."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/S0007-1536(80)80053-2","article-title":"Fusarium heterosporum and Claviceps purpurea on Lolium perenne","volume":"74","author":"Gay","year":"1980","journal-title":"Trans. Br. Mycol. Soc."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1016\/S0007-1536(83)80032-1","article-title":"Fusarium heterosporum, a highly specialized hyperparasite of Claviceps purpurea","volume":"80","author":"Hornok","year":"1983","journal-title":"Trans. Br. Mycol. Soc."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/S0269-915X(09)80673-3","article-title":"Fusarium heterosporum growing on ergots (Claviceps purpurea) in spikelets of common cord-grass (Spartina anglica) in the Isle of Wight","volume":"8","author":"Preece","year":"1994","journal-title":"Mycologist"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1046\/j.1469-8137.1998.00123.x","article-title":"The long-term epidemic of Claviceps purpurea on Spartina anglica in Poole Harbour: Pattern of infection, effects on seed production and the role of Fusarium heterosporum","volume":"138","author":"Raybould","year":"1998","journal-title":"New Phytol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"66","DOI":"10.17221\/48\/2009-PPS","article-title":"Effect of the mycoflora of ergot (Claviceps purpurea) sclerotia on their viability","volume":"46","year":"2010","journal-title":"Plant Prot. Sci."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1016\/j.fgb.2012.12.004","article-title":"Phylogenetic analyses of RPB1 and RPB2 support a middle Cretaceous origin for a clade comprising all agriculturally and medically important fusaria","volume":"52","author":"Rooney","year":"2013","journal-title":"Fungal Genet. Biol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"1597","DOI":"10.1094\/PDIS-09-21-2035-SR","article-title":"DNA Sequence-Based Identification of Fusarium: A Work in Progress","volume":"106","author":"Whitaker","year":"2022","journal-title":"Plant Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"2044","DOI":"10.1073\/pnas.95.5.2044","article-title":"Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies","volume":"95","author":"Kistler","year":"1998","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"1799","DOI":"10.1093\/oxfordjournals.molbev.a026092","article-title":"Phylogenetic relationships among ascomycetes: Evidence from an RNA polymerase II subunit","volume":"16","author":"Liu","year":"1999","journal-title":"Mol. Biol. Evol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1139\/cjb-2012-0178","article-title":"Multigene differences between Microdochium nivale and Microdochium majus","volume":"91","author":"Jewell","year":"2013","journal-title":"Botany"},{"key":"ref_21","unstructured":"(2026, April 10). FUSARIOID-ID Database\u2013Food, Fibre & Health. Available online: https:\/\/www.fusarium.org\/."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1547","DOI":"10.1093\/molbev\/msy096","article-title":"MEGA X: Molecular evolutionary genetics analysis across computing platforms","volume":"35","author":"Kumar","year":"2018","journal-title":"Mol. Biol. Evol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Lord, E., Leclercq, M., Boc, A., Diallo, A.B., and Makarenkov, V. (2012). Armadillo 1.1: An original workflow platform for designing and conducting phylogenetic analysis and simulations. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0029903"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1530","DOI":"10.1093\/molbev\/msaa015","article-title":"IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era","volume":"37","author":"Minh","year":"2020","journal-title":"Mol. Biol. Evol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"434","DOI":"10.1080\/00275514.1998.12026929","article-title":"New Fusarium species and combinations within the Gibberella fujikuroi species complex","volume":"90","author":"Nirenberg","year":"1998","journal-title":"Mycologia"},{"key":"ref_26","unstructured":"Nirenberg, H. (1976). Untersuchungen \u00fcber die Morphologische und Biologische Differenzierung in der Fusarium-Section Liseola, Mitteilungen der Biologischen Bundesanstalt f\u00fcr Land- und Forstwirtschaft."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"4419","DOI":"10.1128\/AEM.70.8.4419-4423.2004","article-title":"Mating type sequences in asexually reproducing Fusarium species","volume":"70","author":"Moretti","year":"2004","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"145","DOI":"10.1016\/j.chroma.2014.08.037","article-title":"Optimization and validation of a quantitative liquid chromatography-tandem mass spectrometric method covering 295 bacterial and fungal metabolites including all regulated mycotoxins in four model food matrices","volume":"1362","author":"Sulyok","year":"2014","journal-title":"J. Chromatogr. A"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"115","DOI":"10.3114\/sim.2011.68.05","article-title":"A revision of Cyanonectria and Geejayessia gen. nov., and related species with Fusarium-like anamorphs","volume":"8","author":"Schroers","year":"2011","journal-title":"Stud. Mycol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"149","DOI":"10.1007\/BF01103339","article-title":"Sexual fertility of forty Fusarium strains from the European Fusarium sambucinum project","volume":"129","author":"Desjardins","year":"1995","journal-title":"Mycopathologia"},{"key":"ref_31","unstructured":"Summerell, B.A., Leslie, J.F., Backhouse, D., and Bryden, W.L. (2001). Perithecial species of Fusarium. Fusarium: Paul E. Nelson Memorial Symposium, APS Press."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"377","DOI":"10.1111\/j.1364-3703.2004.00237.x","article-title":"Claviceps purpurea: Molecular aspects of a unique pathogenic lifestyle","volume":"5","author":"Tudzynski","year":"2004","journal-title":"Mol. Plant Pathol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"4039","DOI":"10.1128\/aem.62.11.4039-4043.1996","article-title":"Production of fusaric acid by Fusarium species","volume":"62","author":"Bacon","year":"1996","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"324","DOI":"10.1021\/np50015a015","article-title":"The isolation and identification of several trichothecene mycotoxins from Fusarium heterosporum","volume":"44","author":"Cole","year":"1981","journal-title":"J. Nat. Prod."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"373","DOI":"10.1146\/annurev-phyto-020620-102825","article-title":"Mycotoxin production in Fusarium according to contemporary species concepts","volume":"59","author":"Munkvold","year":"2021","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"186","DOI":"10.1039\/b413523g","article-title":"Equisetin biosynthesis in Fusarium heterosporum","volume":"2","author":"Sims","year":"2005","journal-title":"Chem. Commun."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Shi, W., Tan, Y., Wang, S., Gardiner, D.M., De Saeger, S., Liao, Y., Wang, C., Fan, Y., Wang, Z., and Wu, A. (2017). Mycotoxigenic potentials of Fusarium species in various culture matrices revealed by mycotoxin profiling. Toxins, 9.","DOI":"10.3390\/toxins9010006"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"35","DOI":"10.1007\/BF02945091","article-title":"Moniliformin production in the genus Fusarium","volume":"14","author":"Nirenberg","year":"1998","journal-title":"Mycotoxin Res."},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Fraeyman, S., Croubels, S., Devreese, M., and Antonissen, G. (2017). Emerging Fusarium and Alternaria mycotoxins: Occurrence, toxicity and toxicokinetics. Toxins, 9.","DOI":"10.3390\/toxins9070228"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1002\/jsfa.9140","article-title":"Fusarium species and moniliformin occurrence in sorghum grains used as ingredient for animal feed in Argentina","volume":"99","author":"Pena","year":"2019","journal-title":"J. Sci. Food Agric."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"323","DOI":"10.1080\/19393210.2025.2519718","article-title":"Moniliformin in cereal grains available in the Japanese market","volume":"18","author":"Aoki","year":"2025","journal-title":"Food Addit. Contam. Part B"},{"key":"ref_42","doi-asserted-by":"crossref","unstructured":"Woo, S.Y., Lee, S.Y., Park, S.B., Kim, S.E., Kang, Y.W., and Chun, H.S. (2025). Assessment of natural occurrence and risk of the emerging mycotoxin moniliformin in South Korea. Toxins, 17.","DOI":"10.3390\/toxins17020050"},{"key":"ref_43","first-page":"5082","article-title":"Scientific opinion on the risks to human and animal health related to the presence of moniliformin in food and feed","volume":"16","author":"Knutsen","year":"2018","journal-title":"EFSA J."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1080\/026520399283849","article-title":"The effects of cereal substrate and temperature on production of beauvericin, moniliformin and fusaproliferin by Fusarium subglutinans ITEM-1434","volume":"16","author":"Kostecki","year":"1999","journal-title":"Food Addit. Contam."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/j.ijfoodmicro.2010.07.015","article-title":"Mycotoxin production of selected Fusarium species at different culture conditions","volume":"143","author":"Kokkonen","year":"2010","journal-title":"Int. J. Food Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/j.ijfoodmicro.2019.02.016","article-title":"Mycotoxins produced by Fusarium proliferatum and F. pseudonygamai on maize, sorghum and pearl millet grains in vitro","volume":"296","author":"Vismer","year":"2019","journal-title":"Int. J. Food Microbiol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"112312","DOI":"10.1016\/j.postharvbio.2023.112312","article-title":"Fusarium proliferatum secondary metabolite profile in vitro depends on the origin of the isolates and is clearly reduced in stored garlic","volume":"200","author":"Gasser","year":"2023","journal-title":"Postharvest Biol. Technol."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/6\/416\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T16:53:40Z","timestamp":1780937620000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/6\/416"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,8]]},"references-count":47,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2026,6]]}},"alternative-id":["jof12060416"],"URL":"https:\/\/doi.org\/10.3390\/jof12060416","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,8]]}}}