{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:19:39Z","timestamp":1785435579127,"version":"3.56.0"},"reference-count":30,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,6,24]],"date-time":"2026-06-24T00:00:00Z","timestamp":1782259200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000974","name":"Australian Centre for International Agricultural Research","doi-asserted-by":"publisher","award":["CROP\/2023\/186"],"award-info":[{"award-number":["CROP\/2023\/186"]}],"id":[{"id":"10.13039\/501100000974","id-type":"DOI","asserted-by":"publisher"}]},{"award":["CROP\/2023\/186"],"award-info":[{"award-number":["CROP\/2023\/186"]}],"id":[{"id":"https:\/\/ror.org\/04wyg6495","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Aflatoxins are carcinogenic secondary metabolites produced by Aspergillus flavus and Aspergillus parasiticus. These two fungi are ubiquitous in soil and are often found in agricultural fields. Four aflatoxin variants commonly found in infected crops are: AFB1, AFB2, AFG1, and AFG2. Aspergillus parasiticus can produce all aflatoxin variants, with A. flavus only able to produce the aflatoxin B variants. Production of aflatoxins typically occurs as pre-harvest contamination in the Australian peanut-growing regions of Queensland. This study analysed geographic variations in aflatoxin component variants using the HPLC method for the 2020\u20132024 season peanuts. Aflatoxin-G was found as most common aflatoxin variant across three of the four peanut-growing regions. This study also evaluated diversity of A. flavus and A. parasiticus across the four peanut-growing regions, using post-harvest soil samples from the 2023\u20132024 growing season. Aspergillus parasiticus was found to be most prevalent (97% isolates) across the regions, whereas A. flavus was least prevalent (3% isolates) and only found in the Tolga region. The North Burnett had no Aspergillus colonies identified from the soil samples in the current year of collection. The data suggests the aflatoxin G variant is most predominant in Australian peanuts and also that there is large variation between the growing regions for prevalence of Aspergillus species.<\/jats:p>","DOI":"10.3390\/jof12070463","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T03:20:21Z","timestamp":1782357621000},"page":"463","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Geographic Profiling of Aspergillus Species and Aflatoxin Variants Across Peanut-Growing Regions of Queensland Australia"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0001-2830-0895","authenticated-orcid":false,"given":"Rebecca","family":"Payne","sequence":"first","affiliation":[{"name":"Centre for Crop Health, School of Science, Engineering and Digital Technologies, University of Southern Queensland, Toowoomba, QLD 4350, Australia"},{"name":"School of Health, Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD 4701, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5290-1781","authenticated-orcid":false,"given":"Dante L.","family":"Adorada","sequence":"additional","affiliation":[{"name":"Centre for Crop Health, School of Science, Engineering and Digital Technologies, University of Southern Queensland, Toowoomba, QLD 4350, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Graeme C.","family":"Wright","sequence":"additional","affiliation":[{"name":"Centre for Crop Health, School of Science, Engineering and Digital Technologies, University of Southern Queensland, Toowoomba, QLD 4350, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8095-3685","authenticated-orcid":false,"given":"Surya","family":"Bhattarai","sequence":"additional","affiliation":[{"name":"Centre for Crop Health, School of Science, Engineering and Digital Technologies, University of Southern Queensland, Toowoomba, QLD 4350, Australia"},{"name":"School of Health, Medical and Applied Sciences, Central Queensland University, Rockhampton, QLD 4701, Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,24]]},"reference":[{"key":"ref_1","unstructured":"Google (2024, July 26). Soil Sampling Locations. Available online: https:\/\/www.google.com\/maps."},{"key":"ref_2","unstructured":"Bureau of Meteorology (2025, June 02). Climate Data Online, Available online: http:\/\/www.bom.gov.au\/climate\/data\/."},{"key":"ref_3","unstructured":"Bureau of Meteorology (2024, October 15). Climate Classification Maps, Available online: http:\/\/www.bom.gov.au\/climate\/maps\/averages\/climate-classification\/?maptype=kpngrp."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"2372","DOI":"10.1111\/ppa.13982","article-title":"Two new qPCR assays for detecting and quantifying the Aspergillus flavus and Aspergillus parasiticus clades in maize kernels","volume":"73","author":"Leharanger","year":"2024","journal-title":"Plant Pathol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"205","DOI":"10.1016\/j.foodcont.2012.11.023","article-title":"Mycotoxin production in major crops as influenced by growing, harvesting, storage and processing, with emphasis on the achievement of Food Safety Objectives","volume":"32","author":"Pitt","year":"2013","journal-title":"Food Control"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1111\/j.1365-2672.1994.tb04418.x","article-title":"Detection of toxigenic isolates of Aspergillus flavus and related species on coconut cream agar","volume":"76","author":"Dyer","year":"1994","journal-title":"J. Appl. Bacteriol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1383","DOI":"10.1016\/j.sjbs.2020.11.073","article-title":"Molecular profile of aflatoxigenic and non-aflatoxigenic isolates of Aspergillus flavus isolated from stored maize","volume":"28","author":"Shehata","year":"2021","journal-title":"Saudi J. Biol. Sci."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"1787","DOI":"10.1080\/19440049.2018.1488276","article-title":"Evaluation of aflatoxin and Aspergillus sp. contamination in raw peanuts and peanut-based products along this supply chain in Malaysia","volume":"35","author":"Norlia","year":"2018","journal-title":"Food Addit. Contam. Part A"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"330","DOI":"10.1016\/j.ijfoodmicro.2006.09.007","article-title":"Studies on Aspergillus section Flavi isolated from maize in northern Italy","volume":"113","author":"Giorni","year":"2007","journal-title":"Int. J. Food Microbiol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1143","DOI":"10.1007\/s00203-020-01822-1","article-title":"Molecular profile of non-aflatoxigenic phenotype in native strains of Aspergillus flavus","volume":"202","author":"Rao","year":"2020","journal-title":"Arch. Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Pitt, J.I., and Hocking, A.D. (2022). Fungi and Food Spoilage, Springer. [4th ed.].","DOI":"10.1007\/978-3-030-85640-3"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"101","DOI":"10.1016\/j.foodres.2017.02.006","article-title":"The biodiversity of Aspergillus section Flavi and aflatoxins in the Brazilian peanut production chain","volume":"94","author":"Martins","year":"2017","journal-title":"Food Res. Int."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"767","DOI":"10.1080\/00275514.1998.12026969","article-title":"Soil populations of Aspergillus species from section Flavi along a transect through peanut-growing regions of the United States","volume":"90","author":"Horn","year":"1998","journal-title":"Mycologia"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"133","DOI":"10.1007\/s12550-010-0048-5","article-title":"Mycotoxigenic fungi in peanuts from different geographic regions of Egypt","volume":"26","author":"Sultan","year":"2010","journal-title":"Mycotoxin Res."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"991","DOI":"10.3390\/toxins4110991","article-title":"Toxigenic potential of Aspergillus species occurring on maize kernels from two agro-ecological zones in Kenya","volume":"4","author":"Okoth","year":"2012","journal-title":"Toxins"},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Zhang, C., Selvaraj, J.N., Yang, Q., and Liu, Y. (2017). A Survey of Aflatoxin-Producing Aspergillus sp. from Peanut Field Soils in Four Agroecological Zones of China. Toxins, 9.","DOI":"10.3390\/toxins9010040"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"257","DOI":"10.1007\/s11046-009-9221-9","article-title":"Survey of Vietnamese peanuts, corn and soil for the presence of Aspergillus flavus and Aspergillus parasiticus","volume":"168","author":"Kennedy","year":"2009","journal-title":"Mycopathologia"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"37","DOI":"10.1016\/j.soilbio.2008.09.013","article-title":"Distribution of Aspergillus section Flavi in soils of maize fields in three agroecological zones of Nigeria","volume":"41","author":"Donner","year":"2009","journal-title":"Soil. Biol. Biochem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1007\/s11046-005-0242-8","article-title":"A survey on distribution of Aspergillus section Flavi in corn field soils in Iran: Population patterns based on aflatoxins, cyclopiazonic acid and sclerotia production","volume":"161","author":"Allameh","year":"2006","journal-title":"Mycopathologia"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"21","DOI":"10.1080\/15572536.2003.11833245","article-title":"Biogeography of Aspergillus Species in Soil and Litter","volume":"94","author":"Klich","year":"2002","journal-title":"Mycologia"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"233","DOI":"10.1007\/s11046-006-0059-0","article-title":"Mycotoxins in Australia: Biocontrol of aflatoxin in peanuts","volume":"162","author":"Pitt","year":"2006","journal-title":"Mycopathologia"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Mamo, F.T., Abate, B.A., Zheng, Y., Nie, C., He, M., and Liu, Y. (2021). Distribution of Aspergillus Fungi and Recent Aflatoxin Reports, Health Risks, and Advances in Developments of Biological Mitigation Strategies in China. Toxins, 13.","DOI":"10.3390\/toxins13100678"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Adhikari, B.N., Callicott, K.A., and Cotty, P.J. (2021). Conservation and Loss of a Putative Iron Utilization Gene Cluster among Genotypes of Aspergillus flavus. Microorganisms, 9.","DOI":"10.3390\/microorganisms9010137"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.fgb.2018.04.009","article-title":"Identification and functional analysis of the aspergillic acid gene cluster in Aspergillus flavus","volume":"116","author":"Lebar","year":"2018","journal-title":"Fungal Genet. Biol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1565","DOI":"10.1111\/ppa.13067","article-title":"Distribution and genetic diversity among Aspergillus flavus isolates across three agro-ecologies essential for maize cultivation in Ghana","volume":"68","author":"Dadzie","year":"2019","journal-title":"Plant Pathol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1842","DOI":"10.1016\/j.soilbio.2010.06.025","article-title":"Crop rotation and soil temperature influence the community structure of Aspergillus flavus in soil","volume":"42","author":"Cotty","year":"2010","journal-title":"Soil Biol. Biochem."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Nji, Q.N., Babalola, O.O., and Mwanza, M. (2023). Soil Aspergillus Species, Pathogenicity and Control Perspectives. J. Fungi, 9.","DOI":"10.3390\/jof9070766"},{"key":"ref_28","doi-asserted-by":"crossref","unstructured":"Pfliegler, W.P., P\u00f3csi, I., Gy\u0151ri, Z., and Pusztahelyi, T. (2020). The Aspergilli and Their Mycotoxins: Metabolic Interactions With Plants and the Soil Biota. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02921"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"e02782-18","DOI":"10.1128\/mBio.02782-18","article-title":"Fitness Cost of Aflatoxin Production in Aspergillus flavus When Competing with Soil Microbes Could Maintain Balancing Selection","volume":"10","author":"Drott","year":"2019","journal-title":"mBio"},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Pitt, J.I., and Hocking, A.D. (2022). Methods for Enumeration, Isolation and Identification. Fungi and Food Spoilage, Springer International Publishing.","DOI":"10.1007\/978-3-030-85640-3_4"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/7\/463\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T04:16:44Z","timestamp":1782361004000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/7\/463"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,24]]},"references-count":30,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["jof12070463"],"URL":"https:\/\/doi.org\/10.3390\/jof12070463","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,24]]}}}