{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T14:50:18Z","timestamp":1782399018280,"version":"3.54.5"},"reference-count":53,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T00:00:00Z","timestamp":1782345600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Henan Provincial Science and Technology Major Project","award":["221100110100"],"award-info":[{"award-number":["221100110100"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Wheat leaf rust, caused by Puccinia triticina Erikss. (Pt), is a major foliar disease that poses a significant threat to wheat production in Henan Province, a major wheat-growing region of China. Elucidating the population genetic structure of Pt is critical for predicting pathogen dispersal trends and guiding disease management. In this study, 384 Pt isolates collected from 13 locations in Henan were genotyped using 11 polymorphic simple sequence repeat (SSR) loci. A total of 204 multilocus genotypes (MLGs) were identified. The Sanmenxia (SMX) population exhibited the highest genetic diversity (H = 3.42; G = 30.12; \u03bb = 0.967), while Shangqiu (SQ) showed the lowest (H = 2.09; G = 4.59; \u03bb = 0.782). Analysis of molecular variance (AMOVA) revealed that 87% of the total genetic variation occurred within populations and 13% among populations. Population structure analyses consistently separated the 13 populations into two genetic clusters, with Xinyang (XY) and SQ forming one distinct group and the remaining 11 populations from the western, central, and northern regions constituting the other. The relative migration network further supported this pattern, showing a highly interconnected network among the central and western populations, but with XY and SQ forming an isolated subnetwork.<\/jats:p>","DOI":"10.3390\/jof12070468","type":"journal-article","created":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T12:37:55Z","timestamp":1782391075000},"page":"468","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Genetic Analysis Reveals Relationships Among Populations of Puccinia triticina from Henan Province of China"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6515-1609","authenticated-orcid":false,"given":"Shuhe","family":"Wang","sequence":"first","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Province Engineering Technology Research Center of Green Plant Protection, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yang","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Province Engineering Technology Research Center of Green Plant Protection, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuxin","family":"Gu","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Province Engineering Technology Research Center of Green Plant Protection, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jinhang","family":"Luo","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Province Engineering Technology Research Center of Green Plant Protection, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shengming","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Horticulture and Plant Protection, Henan University of Science and Technology, Luoyang 471023, China"},{"name":"Henan Province Engineering Technology Research Center of Green Plant Protection, Luoyang 471023, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,25]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Grote, U., Fasse, A., Nguyen, T.T., and Erenstein, O. (2021). Food Security and the Dynamics of Wheat and Maize Value Chains in Africa and Asia. Front. Sustain. Food Syst., 4.","DOI":"10.3389\/fsufs.2020.617009"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"100446","DOI":"10.1016\/j.gfs.2020.100446","article-title":"The threat to global food security from wheat rust: Ethical and historical issues in fighting crop diseases and preserving genetic diversity","volume":"26","author":"Lapthorn","year":"2020","journal-title":"Glob. Food Secur."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Maulenbay, A., and Rsaliyev, A. (2024). Fungal Disease Tolerance with a Focus on Wheat: A Review. J. Fungi, 10.","DOI":"10.3390\/jof10070482"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"102310","DOI":"10.1016\/j.mib.2023.102310","article-title":"Safeguarding wheat yields from cereal fungal invaders in the postgenomic era","volume":"73","author":"Minter","year":"2023","journal-title":"Curr. Opin. Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"537","DOI":"10.1016\/j.funbio.2020.02.013","article-title":"The progress of leaf rust research in wheat","volume":"124","author":"Prasad","year":"2020","journal-title":"Fungal Biol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"563","DOI":"10.1111\/j.1364-3703.2008.00487.x","article-title":"Wheat leaf rust caused by Puccinia triticina","volume":"9","author":"Bolton","year":"2008","journal-title":"Mol. Plant Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1094\/PHYTO-02-20-0032-R","article-title":"A Probabilistic Bio-Economic Assessment of the Global Consequences of Wheat Leaf Rust","volume":"110","author":"Chai","year":"2020","journal-title":"Phytopathology"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"143","DOI":"10.1007\/s10681-011-0361-x","article-title":"Global status of wheat leaf rust caused by Puccinia triticina","volume":"179","author":"Singh","year":"2011","journal-title":"Euphytica"},{"key":"ref_9","doi-asserted-by":"crossref","unstructured":"Reynolds, M.P., and Braun, H.-J. (2022). Global Trends in Wheat Production, Consumption and Trade. Wheat Improvement: Food Security in a Changing Climate, Springer International Publishing.","DOI":"10.1007\/978-3-030-90673-3_1"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"127066","DOI":"10.1016\/j.eja.2023.127066","article-title":"Impact of climate change on wheat production in China","volume":"153","author":"Sun","year":"2024","journal-title":"Eur. J. Agron."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"6","DOI":"10.1186\/s42483-023-00159-z","article-title":"Fighting wheat rusts in China: A look back and into the future","volume":"5","author":"Zhao","year":"2023","journal-title":"Phytopathol. Res."},{"key":"ref_12","unstructured":"Li, Z., and Zeng, S. (2002). Wheat Rusts in China, China Agriculture Press."},{"key":"ref_13","first-page":"77","article-title":"Analysis of the genetic diversity of Puccinia triticina isolated from different wheat cultivars","volume":"47","author":"Zhang","year":"2018","journal-title":"J. Henan Agric. Sci."},{"key":"ref_14","first-page":"29","article-title":"Epidemiological analysis of major wheat diseases in Henan during 2015","volume":"36","author":"Peng","year":"2016","journal-title":"China Plant Prot."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"455","DOI":"10.1094\/PDIS-05-19-1051-RE","article-title":"Race and virulence analysis of Puccinia triticina in China in 2014 and 2015","volume":"104","author":"Zhang","year":"2020","journal-title":"Plant Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"134734","DOI":"10.1016\/j.jclepro.2022.134734","article-title":"Effects of projected climate change on winter wheat yield in Henan, China","volume":"379","author":"Zhang","year":"2022","journal-title":"J. Clean. Prod."},{"key":"ref_17","unstructured":"Xia, X. (2021). Over-Summering Regionalization and Population Genetic Structure of Puccinia triticina in China. [Master\u2019s Thesis, Institute of Plant Protection Graduate School]."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1146\/annurev-phyto-080614-115936","article-title":"Genome-Enabled Analysis of Plant-Pathogen Migration","volume":"53","author":"Goss","year":"2015","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"342","DOI":"10.1111\/ppa.13657","article-title":"SSR genotypes of Puccinia triticina in 10 provinces of China reveal a possible source of the wheat leaf rust fungus","volume":"72","author":"Xia","year":"2022","journal-title":"Plant Pathol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"427","DOI":"10.1094\/PHYTO.2000.90.4.427","article-title":"Virulence and Molecular Polymorphism in International Collections of the Wheat Leaf Rust Fungus Puccinia triticina","volume":"90","author":"Kolmer","year":"2000","journal-title":"Phytopathology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"111","DOI":"10.1111\/j.1439-0434.2009.01573.x","article-title":"Molecular Polymorphism of the Wheat Leaf Rust Fungus in Morocco Using Amplified Fragment Length Polymorphism","volume":"158","author":"Bouftass","year":"2010","journal-title":"J. Phytopathol."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Zhang, L., Zhao, P., Meng, Q., Yan, H., and Liu, D. (2024). The Migration, Diversity, and Evolution of Puccinia triticina in China. Plants, 13.","DOI":"10.3390\/plants13172438"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Xu, Z., Li, H., Xia, X., Liu, B., Gao, L., Chen, W., and Liu, T. (2022). SSR Genotypes of the Puccinia triticina in 15 Provinces of China Indicate Regional Migration in One Season from East to West and South to North. Agronomy, 12.","DOI":"10.3390\/agronomy12123068"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s13313-018-0542-3","article-title":"Microsatellite analysis of Puccinia triticina from Triticum and Aegilops hosts","volume":"47","author":"Gultyaeva","year":"2018","journal-title":"Australas. Plant Pathol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1094\/PHYTO-10-18-0411-R","article-title":"Multilocus Genotypes of the Wheat Leaf Rust Fungus Puccinia triticina in Worldwide Regions Indicate Past and Current Long-Distance Migration","volume":"109","author":"Kolmer","year":"2019","journal-title":"Phytopathology"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Zhang, L., Zhang, L., Meng, Q., Yan, H., and Liu, D. (2023). Virulence and molecular genetic diversity, variation, and evolution of the Puccinia triticina population in Hebei Province of China from 2001 to 2010. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1095677"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"786","DOI":"10.1094\/PHYTO-10-16-0388-R","article-title":"Genetic Differentiation of the Wheat Leaf Rust Fungus Puccinia triticina in Pakistan and Genetic Relationship to Other Worldwide Populations","volume":"107","author":"Kolmer","year":"2017","journal-title":"Phytopathology"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1779","DOI":"10.1016\/S2095-3119(18)61923-9","article-title":"Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences","volume":"17","author":"Liu","year":"2018","journal-title":"J. Integr. Agric."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1135","DOI":"10.1007\/s10658-020-01956-4","article-title":"Population genetic structures of Puccinia triticina in five provinces of China","volume":"156","author":"Ma","year":"2020","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_30","first-page":"89","article-title":"Genetic diversity of Puccinia triticina by SSR in some regions of China","volume":"21","author":"Xu","year":"2013","journal-title":"J. Agric. Biotechnol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1186\/s42483-023-00163-3","article-title":"Molecular evidences for population differentiation and the migration from south to north of Puccinia triticina in eastern China","volume":"5","author":"Li","year":"2023","journal-title":"Phytopathol. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"501","DOI":"10.1094\/PDIS-01-12-0072-RE","article-title":"Population Genetic Structures of Puccinia striiformis in Ningxia and Gansu Provinces of China","volume":"97","author":"Liang","year":"2013","journal-title":"Plant Dis."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"442","DOI":"10.1111\/jph.12578","article-title":"Development of a sequence-characterized amplified region marker using inter-simple sequence repeats for detection of Puccinia striiformis f. sp. tritici","volume":"165","author":"Wang","year":"2017","journal-title":"J. Phytopathol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"240","DOI":"10.1186\/1756-0500-4-240","article-title":"A rapid genotyping method for an obligate fungal pathogen, Puccinia striiformis f.sp. tritici, based on DNA extraction from infected leaf and Multiplex PCR genotyping","volume":"4","author":"Ali","year":"2011","journal-title":"BMC Res. Notes"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1046\/j.1471-8286.2003.00350.x","article-title":"Isolation of 12 microsatellite loci, using an enrichment protocol, in the phytopathogenic fungus Puccinia triticina","volume":"3","author":"Duan","year":"2003","journal-title":"Mol. Ecol. Notes"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1111\/j.1471-8286.2007.01686.x","article-title":"Development of simple sequence repeat markers for the plant pathogenic rust fungus Puccinia triticina","volume":"7","author":"Szabo","year":"2007","journal-title":"Mol. Ecol. Notes"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"2537","DOI":"10.1093\/bioinformatics\/bts460","article-title":"GenAlEx 6.5: Genetic analysis in Excel. Population genetic software for teaching and research\u2014An update","volume":"28","author":"Peakall","year":"2012","journal-title":"Bioinformatics"},{"key":"ref_38","doi-asserted-by":"crossref","unstructured":"Kamvar, Z.N., Tabima, J.F., and Gr\u00fcnwald, N.J. (2014). Poppr: An R package for genetic analysis of populations with clonal, partially clonal, and\/or sexual reproduction. PeerJ, 2.","DOI":"10.7717\/peerj.281"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"2128","DOI":"10.1093\/bioinformatics\/bti282","article-title":"PowerMarker: An integrated analysis environment for genetic marker analysis","volume":"21","author":"Liu","year":"2005","journal-title":"Bioinformatics"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"485","DOI":"10.1111\/2041-210X.13748","article-title":"DA: Population structure inference using discriminant analysis","volume":"13","author":"Qin","year":"2021","journal-title":"Methods Ecol. Evol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1403","DOI":"10.1093\/bioinformatics\/btn129","article-title":"adegenet: A R package for the multivariate analysis of genetic markers","volume":"24","author":"Jombart","year":"2008","journal-title":"Bioinformatics"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"94","DOI":"10.1186\/1471-2156-11-94","article-title":"Discriminant analysis of principal components a new method for the analysis of genetically structured populations","volume":"11","author":"Jombart","year":"2010","journal-title":"BMC Genet."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"945","DOI":"10.1093\/genetics\/155.2.945","article-title":"Inference of Population Structure Using Multilocus Genotype Data","volume":"155","author":"Pritchard","year":"2000","journal-title":"Genetics"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2611","DOI":"10.1111\/j.1365-294X.2005.02553.x","article-title":"Detecting the number of clusters of individuals using the software structure: A simulation study","volume":"14","author":"Evanno","year":"2005","journal-title":"Mol. Ecol."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"176","DOI":"10.1111\/1755-0998.12719","article-title":"StructureSelector: A web-based software to select and visualize the optimal number of clusters using multiple methods","volume":"18","author":"Li","year":"2017","journal-title":"Mol. Ecol. Resour."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.1111\/1755-0998.12387","article-title":"Clumpak: A program for identifying clustering modes and packaging population structure inferences across K","volume":"15","author":"Kopelman","year":"2015","journal-title":"Mol. Ecol. Resour."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"3461","DOI":"10.1002\/ece3.2096","article-title":"Directional genetic differentiation and relative migration","volume":"6","author":"Sundqvist","year":"2016","journal-title":"Ecol. Evol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"75","DOI":"10.1016\/j.tpb.2014.02.003","article-title":"On the transition of genetic differentiation from isolation to panmixia: What we can learn from and","volume":"93","author":"Alcala","year":"2014","journal-title":"Theor. Popul. Biol."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Luchi, N. (2022). Genetic Analysis of Plant Pathogens Natural Populations. Plant Pathology, Springer.","DOI":"10.1007\/978-1-0716-2517-0"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Li, H., Zhao, N., Zhang, Q., Huang, L., Zhang, H., Gao, L., Chen, W., and Liu, T. (2025). Genetic and wind field analysis of wheat leaf rust (Puccinia triticina) dispersal: From winter sources in Gansu and Shaanxi to summer epidemics in China. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1558898"},{"key":"ref_51","doi-asserted-by":"crossref","unstructured":"Sugiyama, M. (2006, January 25\u201329). Local Fisher Discriminant Analysis for Supervised Dimensionality Reduction. Proceedings of the 23rd International Conference on Machine Learning, Pittsburgh, PA, USA.","DOI":"10.1145\/1143844.1143958"},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Qin, X., Chiang, C.W.K., and Gaggiotti, O.E. (2022). KLFDAPC: A supervised machine learning approach for spatial genetic structure analysis. Brief. Bioinform., 23.","DOI":"10.1093\/bib\/bbac202"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"2130","DOI":"10.1038\/s41564-023-01494-9","article-title":"Nuclear exchange generates population diversity in the wheat leaf rust pathogen Puccinia triticina","volume":"8","author":"Sperschneider","year":"2023","journal-title":"Nat. Microbiol."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/7\/468\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,25]],"date-time":"2026-06-25T14:15:56Z","timestamp":1782396956000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/7\/468"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,25]]},"references-count":53,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["jof12070468"],"URL":"https:\/\/doi.org\/10.3390\/jof12070468","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,25]]}}}