{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T11:45:08Z","timestamp":1782819908235,"version":"3.54.5"},"reference-count":27,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T00:00:00Z","timestamp":1782777600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"European Union\u2019s Horizon 2020 research and innovation program","award":["101037796"],"award-info":[{"award-number":["101037796"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Agaricus bisporus is highly valued for its sensory and nutritional aspects, but it is highly perishable because of its intense postharvest metabolism. In this study, we looked at how white button mushrooms responded to three thermal regimes including constant refrigeration (4 \u00b0C), ambient storage (20 \u00b0C), and repeated temperature stress (RTS). The investigations were combined with an innovative smart packaging called Store Box (SB). Using an integrated volatilomics approach combining both gas chromatography\u2013mass spectrometry (GC-MS) and proton transfer reaction time of flight mass spectrometry (PTR-ToF-MS), we tracked volatile compounds from intact mushrooms and internal tissues and linked these with weight loss and CO2 production. According to the results, the SB stabilizes the internal microenvironment by reducing moisture loss and modulating gas exchange through controlled CO2 buildup. This mechanism attenuated the respiratory surge and significantly delayed the rise of spoilage-related markers like methanethiol, acetaldehyde, and hexanal. At the same time, SB preserved freshness indicators such as 1-octen-3-one and benzaldehyde, which normally fade as mushrooms age. The protective effect was especially clear during thermal fluctuations, where SB acted as a metabolic buffer. Overall, this work offers new insights into the volatilome dynamics of Agaricus bisporus and confirms that smart packaging can help offset the damage caused by temperature instability along the supply chain.<\/jats:p>","DOI":"10.3390\/jof12070477","type":"journal-article","created":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T08:26:15Z","timestamp":1782807975000},"page":"477","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Impact of Temperature Regimes and Smart Packaging on Volatilome Evolution During the Shelf Life of Agaricus bisporus"],"prefix":"10.3390","volume":"12","author":[{"given":"Mehdi","family":"Moayedi","sequence":"first","affiliation":[{"name":"Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, San Michele all\u2019Adige, 38010 Trento, Italy"},{"name":"Department of Chemical Sciences, University of Padua, Via Marzolo 1, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9383-9504","authenticated-orcid":false,"given":"Michele","family":"Pedrotti","sequence":"additional","affiliation":[{"name":"Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, San Michele all\u2019Adige, 38010 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iuliia","family":"Khomenko","sequence":"additional","affiliation":[{"name":"Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, San Michele all\u2019Adige, 38010 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3548-9921","authenticated-orcid":false,"given":"Emanuela","family":"Betta","sequence":"additional","affiliation":[{"name":"Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, San Michele all\u2019Adige, 38010 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2392-2705","authenticated-orcid":false,"given":"Andrea","family":"Romano","sequence":"additional","affiliation":[{"name":"Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, San Michele all\u2019Adige, 38010 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4694-4181","authenticated-orcid":false,"given":"Matteo","family":"Tonezzer","sequence":"additional","affiliation":[{"name":"Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, San Michele all\u2019Adige, 38010 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6740-3638","authenticated-orcid":false,"given":"Luca","family":"Cappellin","sequence":"additional","affiliation":[{"name":"Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, San Michele all\u2019Adige, 38010 Trento, Italy"},{"name":"Department of Chemical Sciences, University of Padua, Via Marzolo 1, 35131 Padova, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5715-9686","authenticated-orcid":false,"given":"Franco","family":"Biasioli","sequence":"additional","affiliation":[{"name":"Research and Innovation Centre, Fondazione Edmund Mach, Via Edmund Mach 1, San Michele all\u2019Adige, 38010 Trento, Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,6,30]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"72","DOI":"10.1016\/j.tifs.2018.05.012","article-title":"Recent Advances in Quality Preservation of Postharvest Mushrooms (Agaricus bisporus): A Review","volume":"78","author":"Zhang","year":"2018","journal-title":"Trends Food Sci. Technol."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Dimopoulou, M., Kolonas, A., Mourtakos, S., Androutsos, O., and Gortzi, O. (2022). Nutritional Composition and Biological Properties of Sixteen Edible Mushroom Species. Appl. Sci., 12.","DOI":"10.3390\/app12168074"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1016\/j.postharvbio.2015.03.007","article-title":"Postharvest Application of 4-Methoxy Cinnamic Acid for Extending the Shelf Life of Mushroom (Agaricus bisporus)","volume":"104","author":"Hu","year":"2015","journal-title":"Postharvest Biol. Technol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Tao, M., Zhu, Y., Chen, F., Fang, Y., Han, Y., Yin, G., and Li, N. (2024). Metabolomic and Transcriptomic Analyses Revealed Lipid Differentiation Mechanisms in Agaricus bisporus at Ambient Conditions. J. Fungi, 10.","DOI":"10.20944\/preprints202406.0547.v1"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"285","DOI":"10.1006\/fstl.2000.0657","article-title":"Post-Harvest Treatment with Citric Acid or Hydrogen Peroxide to Extend the Shelf Life of Fresh Sliced Mushrooms","volume":"33","author":"Brennan","year":"2000","journal-title":"LWT-Food Sci. Technol."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"110385","DOI":"10.1016\/j.scienta.2021.110385","article-title":"Inhibition of Browning and Shelf Life Extension of Button Mushroom (Agaricus bisporus) by Ergothioneine Treatment","volume":"288","author":"Qian","year":"2021","journal-title":"Sci. Hortic."},{"key":"ref_7","unstructured":"Thompson, A.K. (2008). Fruit and Vegetables: Harvesting, Handling and Storage, John Wiley & Sons."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"408","DOI":"10.1016\/j.postharvbio.2007.11.007","article-title":"Development of a Moisture Absorber for Packaging of Fresh Mushrooms (Agaricus bisporous)","volume":"48","author":"Mahajan","year":"2008","journal-title":"Postharvest Biol. Technol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"112585","DOI":"10.1016\/j.postharvbio.2023.112585","article-title":"Quality and Microbial Community Changes in Three Kinds of Boletus Wild Mushroom during Cold Storage","volume":"206","author":"Liu","year":"2023","journal-title":"Postharvest Biol. Technol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"e13839","DOI":"10.1111\/jfpp.13839","article-title":"Modified Atmosphere Packaging for Extending the Shelf Life of Fresh Agaricus bisporus","volume":"42","author":"Zalewska","year":"2018","journal-title":"J. Food Process. Preserv."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"e16648","DOI":"10.1111\/jfpp.16648","article-title":"Effect of Micro-Perforated Film Packing on Physicochemical Quality and Volatile Profile of Button Mushroom (Agaricus bisporus) during Postharvest Shelf-Life","volume":"46","author":"Cheng","year":"2022","journal-title":"J. Food Process. Preserv."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/j.fpsl.2017.09.001","article-title":"Shelf Life Extension of White Mushrooms (Agaricus bisporus) by Low Temperatures Conditioning, Modified Atmosphere, and Nanocomposite Packaging Material","volume":"14","author":"Gholami","year":"2017","journal-title":"Food Packag. Shelf Life"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"334","DOI":"10.1111\/j.1365-2621.1995.tb05667.x","article-title":"Fresh Mushroom Quality as Affected by Modified Atmosphere Packaging","volume":"60","author":"Roy","year":"1995","journal-title":"J. Food Sci."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"5568","DOI":"10.1080\/10408398.2022.2155798","article-title":"The Flavors of Edible Mushrooms: A Comprehensive Review of Volatile Organic Compounds and Their Analytical Methods","volume":"64","author":"Zhu","year":"2024","journal-title":"Crit. Rev. Food Sci. Nutr."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Su, T., Chen, Y., Liu, H., Gao, Y., Guo, J., Li, Y., Qi, Y., and Qiu, L. (2022). The Biosynthesis of 1-Octene-3-Ol by a Multifunctional Fatty Acid Dioxygenase and Hydroperoxide Lyase in Agaricus bisporus. J. Fungi, 8.","DOI":"10.3390\/jof8080827"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"524","DOI":"10.37290\/ctnr2641-452X.21:524-530","article-title":"Analysis of Volatile Compounds in Agaricus bisporus by Headspace Solid-Phase Microextraction-Gas Chromatography-Mass Spectrometry","volume":"21","author":"Yu","year":"2023","journal-title":"Curr. Top. Nutraceutical Res."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Xie, L., Guo, S., Rao, H., Lan, B., Zheng, B., and Zhang, N. (2024). Characterization of Volatile Flavor Compounds and Aroma Active Components in Button Mushroom (Agaricus bisporus) across Various Cooking Methods. Foods, 13.","DOI":"10.3390\/foods13050685"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"3709","DOI":"10.1021\/jf8036209","article-title":"Influence of Sporophore Development, Damage, Storage, and Tissue Specificity on the Enzymic Formation of Volatiles in Mushrooms (Agaricus bisporus)","volume":"57","author":"Combet","year":"2009","journal-title":"J. Agric. Food Chem."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"113802","DOI":"10.1016\/j.foodres.2023.113802","article-title":"Non-Destructive Egg Breed Separation Using Advanced VOC Analytical Techniques HSSE-GC-MS, PTR-TOF-MS, and SIFT-MS: Assessment of Performance and Systems\u2019 Complementarity","volume":"176","author":"Corion","year":"2024","journal-title":"Food Res. Int."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"191","DOI":"10.1016\/S0168-1176(97)00281-4","article-title":"On-Line Monitoring of Volatile Organic Compounds at Pptv Levels by Means of Proton-Transfer-Reaction Mass Spectrometry (PTR-MS) Medical Applications, Food Control and Environmental Research","volume":"173","author":"Lindinger","year":"1998","journal-title":"Int. J. Mass Spectrom. Ion Process."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"104729","DOI":"10.1016\/j.idairyj.2020.104729","article-title":"The Good, the Bad and the Aged: Predicting Sensory Quality of Anhydrous Milk Fat by PTR\/SRI-Tof-MS Analysis and Data Mining","volume":"109","author":"Pedrotti","year":"2020","journal-title":"Int. Dairy J."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"66","DOI":"10.3390\/jof7010066","article-title":"High-Throughput Volatilome Fingerprint Using PTR\u2013TOF\u2013MS Shows Species-Specific Patterns in Mortierella and Closely Related Genera","volume":"7","author":"Telagathoti","year":"2021","journal-title":"J. Fungi"},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Wr\u00f3blewski, T., Kami\u0144ska, A., and W\u0142odarkiewicz, A. (2025). Studies on the Emission of Volatile Organic Compounds from Selected Forest Mushrooms of the Genus Lactarius Using Proton-Transfer Reaction Mass Spectrometry. Molecules, 30.","DOI":"10.3390\/molecules30143000"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Panarese, A., Farneti, B., Zanella, A., and Khomenko, I. (2025). Non-Destructive PTR-ToF-MS Profiling of Red Delicious and Granny Smith Apple Volatilomes During Ripening. Agriculture, 15.","DOI":"10.3390\/agriculture15060638"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"Di Pierro, E.A., Franceschi, P., Endrizzi, I., Farneti, B., Poles, L., Masuero, D., Khomenko, I., Trenti, F., Marrano, A., and Vrhovsek, U. (2022). Valorization of Traditional Italian Walnut (Juglans regia L.) Production: Genetic, Nutritional and Sensory Characterization of Locally Grown Varieties in the Trentino Region. Plants, 11.","DOI":"10.3390\/plants11151986"},{"key":"ref_26","doi-asserted-by":"crossref","unstructured":"Chang, C.-K., Cheng, K.-C., Hou, C.-Y., Wu, Y.-S., and Hsieh, C.-W. (2021). Development of Active Packaging to Extend the Shelf Life of Agaricus bisporus by Using Plasma Technology. Polymers, 13.","DOI":"10.3390\/polym13132120"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"566","DOI":"10.1016\/j.foodchem.2019.01.053","article-title":"Odor-Contributing Volatile Compounds of Wild Edible Nordic Mushrooms Analyzed with HS\u2013SPME\u2013GC\u2013MS and HS\u2013SPME\u2013GC\u2013O\/FID","volume":"283","author":"Aisala","year":"2019","journal-title":"Food Chem."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/7\/477\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,6,30]],"date-time":"2026-06-30T10:52:13Z","timestamp":1782816733000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/7\/477"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,6,30]]},"references-count":27,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2026,7]]}},"alternative-id":["jof12070477"],"URL":"https:\/\/doi.org\/10.3390\/jof12070477","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,6,30]]}}}