{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T09:42:46Z","timestamp":1785404566996,"version":"3.56.0"},"reference-count":36,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T00:00:00Z","timestamp":1783468800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006477","name":"National Taiwan University","doi-asserted-by":"publisher","award":["112HT613006"],"award-info":[{"award-number":["112HT613006"]}],"id":[{"id":"10.13039\/501100006477","id-type":"DOI","asserted-by":"publisher"}]},{"award":["112HT613006"],"award-info":[{"award-number":["112HT613006"]}],"id":[{"id":"https:\/\/ror.org\/05bqach95","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Ganoderic acids (GAs) are bioactive lanostane-type triterpenoids produced by Ganoderma lucidum that accumulate predominantly in fruiting bodies, whose long cultivation period limits their practical production. Using Global Natural Products Social Molecular Networking (GNPS), we identified G. lucidum TM701, which accumulated 99 GA derivatives in mycelia and exhibited higher triterpenoid levels and greater chemical diversity than the commercial strain BCRC 36203. Four abundant GAs (GA-Mb\/Mc, GA-S\/Mf, GA-T, and GA-R) were selected as marker compounds for monitoring GA production. A modified two-stage cultivation strategy, combining submerged inoculum preparation with nutrient optimization during static cultivation, increased GA production to 1396 mg L\u22121 GAs in potato dextrose broth supplemented with 2% glucose. Heat treatment revealed interconversion among GA-Mb\/Mc, GA-Mh, and GA-P\/Q, indicating a dehydration-driven stabilization of conjugated diene structures. G. lucidum TM701 mycelia not only provide a platform for triterpenoid production, but also serve as a promising model for elucidating GA biosynthesis and investigating their thermal transformation.<\/jats:p>","DOI":"10.3390\/jof12070500","type":"journal-article","created":{"date-parts":[[2026,7,8]],"date-time":"2026-07-08T17:27:31Z","timestamp":1783531651000},"page":"500","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Enhanced Production and Profiling of Ganoderic Acids in Ganoderma lucidum Mycelia via Two-Stage Cultivation and GNPS-Guided Metabolomics"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2381-503X","authenticated-orcid":false,"given":"Chieh-Hsi","family":"Tsao","sequence":"first","affiliation":[{"name":"Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9769-0914","authenticated-orcid":false,"given":"Hsin-Ya","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6717-4342","authenticated-orcid":false,"given":"Kai-Wen","family":"Cheng","sequence":"additional","affiliation":[{"name":"Leeuwenhoek Laboratories Co., Ltd., Taipei 10688, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Guan-Yuan","family":"Chen","sequence":"additional","affiliation":[{"name":"Department and Graduate Institute of Forensic Medicine, College of Medicine, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hao-Ting","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2892-5326","authenticated-orcid":false,"given":"Cheng-Chih","family":"Hsu","sequence":"additional","affiliation":[{"name":"Leeuwenhoek Laboratories Co., Ltd., Taipei 10688, Taiwan"},{"name":"Department of Chemistry, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nan-Wei","family":"Su","sequence":"additional","affiliation":[{"name":"Department of Agricultural Chemistry, National Taiwan University, Taipei 10617, Taiwan"},{"name":"Department of Biochemical Science and Technology, National Taiwan University, Taipei 10617, Taiwan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,7,8]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1243","DOI":"10.1007\/s00253-010-2871-1","article-title":"Current progress in the study on biosynthesis and regulation of ganoderic acids","volume":"88","author":"Shi","year":"2010","journal-title":"Appl. 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