{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,4]],"date-time":"2026-08-04T04:45:10Z","timestamp":1785818710701,"version":"3.56.0"},"reference-count":23,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012453","name":"China Agricultural Research System","doi-asserted-by":"crossref","award":["CARS-01-38"],"award-info":[{"award-number":["CARS-01-38"]}],"id":[{"id":"10.13039\/501100012453","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Fungi","JoF"],"abstract":"<jats:p>Sexual reproduction of the ascomycetous fungus Magnaporthe oryzae is reported to be regulated by illuminance and temperature, but their temporal interaction during perithecial production remains unclear. Here, we performed full-factorial condition transfer assays (illuminance: low\/0 lx, medium\/1000 lx, or high\/5000 lx; temperature: low\/19 \u00b0C or high\/25 \u00b0C) using a pair of high-fertility M. oryzae isolates collected from green bristlegrass to characterize sequential and combinatorial effects of illuminance and temperature on perithecial production. We found that perithecial production showed a strong positive correlation with illuminance at 19 \u00b0C, and continuous darkness completely abolished their fertility. Early illuminance priming promotes perithecial production in a dose-dependent manner, whereas prolonged initial darkness for over four days results in remarkable reductions in perithecial production. Moreover, pre-transfer at 25 \u00b0C promoted perithecial production under 1000 lx or darkness, and post-transfer at 19 \u00b0C sustained or enhanced perithecial production. We also found that plates initially cultured at 5000 lx maintain high capacity for perithecial production across most post-transfer environments, especially under the post-transfer condition at 19 \u00b0C with over 2000 perithecia per plate. Taken together, our study demonstrates that combinations of high illuminance and low temperature promote perithecial production of the green bristlegrass blast fungus M. oryzae under laboratory conditions, providing alternative insights into the roles of environmental factors in fungal sexual reproduction.<\/jats:p>","DOI":"10.3390\/jof12080564","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T11:50:21Z","timestamp":1785757821000},"page":"564","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["High Illuminance and Low Temperature Promote Perithecial Production of the Green Bristlegrass Blast Fungus Magnaporthe oryzae"],"prefix":"10.3390","volume":"12","author":[{"given":"Jintao","family":"Liu","sequence":"first","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China"},{"name":"Liaoning Province Saline-Alkali Land Utilization Research Institute, Panjin 124010, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zixue","family":"Wu","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huiying","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qifeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Heilongjiang Provincial Plant Inspection and Quarantine Station, Harbin 150036, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haixu","family":"Sang","sequence":"additional","affiliation":[{"name":"Liaoning Province Saline-Alkali Land Utilization Research Institute, Panjin 124010, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6085-9245","authenticated-orcid":false,"given":"Jun","family":"Yang","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Laboratory of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"298","DOI":"10.1128\/MMBR.00005-10","article-title":"The evolution of sex: A perspective from the fungal kingdom","volume":"74","author":"Lee","year":"2010","journal-title":"Microbiol. 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