{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:52:39Z","timestamp":1785761559893,"version":"3.56.0"},"reference-count":27,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T00:00:00Z","timestamp":1785542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Fungi","JoF"],"abstract":"<jats:p>Invasive fungal infections, including candidemia and invasive aspergillosis, remain significant causes of morbidity and mortality in at-risk patient populations. Rapid, accurate diagnosis is critical for timely antifungal therapy and improved clinical outcomes. We evaluated two commercial molecular assays, the CandID Multiplex PCR and AspID Real-Time PCR, for detection of clinically relevant Candida and Aspergillus species, respectively, in at-risk patients in Paraguay. The CandID assay demonstrated strong agreement with blood culture in detecting candidemia. The AspID assay showed high concordance with galactomannan testing, particularly in bronchoalveolar lavage specimens, supporting its role as a complementary diagnostic tool. Our findings underscore the value of integrating molecular diagnostics into routine fungal diagnostic workflows to improve early detection of invasive fungal infections.<\/jats:p>","DOI":"10.3390\/jof12080567","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T11:50:21Z","timestamp":1785757821000},"page":"567","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Evaluation of Molecular Assays for Rapid Diagnosis of Invasive Fungal Infections: Clinical Experience with the Commercial CandID and AspID Real-Time PCR Assays in Paraguay"],"prefix":"10.3390","volume":"12","author":[{"given":"Jos\u00e9","family":"Pereira","sequence":"first","affiliation":[{"name":"Center for Dermatological Diseases, National Leprosy Control Program, Ministry of Public Health and Social Welfare, San Lorenzo 2160, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Melisa","family":"Florent\u00edn","sequence":"additional","affiliation":[{"name":"Center for Dermatological Diseases, National Leprosy Control Program, Ministry of Public Health and Social Welfare, San Lorenzo 2160, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amiliana","family":"Pineda","sequence":"additional","affiliation":[{"name":"Center for Dermatological Diseases, National Leprosy Control Program, Ministry of Public Health and Social Welfare, San Lorenzo 2160, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Desiree","family":"Almir\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto de Previsi\u00f3n Social, Asunci\u00f3n 1209, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gemma","family":"Johnson","sequence":"additional","affiliation":[{"name":"IMMY, Norman, OK 73069, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Monserrat","family":"Aldama","sequence":"additional","affiliation":[{"name":"Center for Dermatological Diseases, National Leprosy Control Program, Ministry of Public Health and Social Welfare, San Lorenzo 2160, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Idalina","family":"Franco","sequence":"additional","affiliation":[{"name":"Center for Dermatological Diseases, National Leprosy Control Program, Ministry of Public Health and Social Welfare, San Lorenzo 2160, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hern\u00e1n","family":"Barrios","sequence":"additional","affiliation":[{"name":"Center for Dermatological Diseases, National Leprosy Control Program, Ministry of Public Health and Social Welfare, San Lorenzo 2160, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7551-0054","authenticated-orcid":false,"given":"Olga","family":"Aldama","sequence":"additional","affiliation":[{"name":"Center for Dermatological Diseases, National Leprosy Control Program, Ministry of Public Health and Social Welfare, San Lorenzo 2160, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0190-3568","authenticated-orcid":false,"given":"Fernando","family":"Ar\u00e9valos","sequence":"additional","affiliation":[{"name":"Center for Dermatological Diseases, National Leprosy Control Program, Ministry of Public Health and Social Welfare, San Lorenzo 2160, Paraguay"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6506-189X","authenticated-orcid":false,"given":"Meredith","family":"Brown","sequence":"additional","affiliation":[{"name":"IMMY, Norman, OK 73069, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tom M.","family":"Chiller","sequence":"additional","affiliation":[{"name":"IMMY, Norman, OK 73069, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8749-9809","authenticated-orcid":false,"given":"Diego H.","family":"C\u00e1ceres","sequence":"additional","affiliation":[{"name":"IMMY, Norman, OK 73069, USA"},{"name":"Emerging Diseases Research Group, Studies in Translational Microbiology and Faculty of Medical Sciences, Universidad del Rosario, Bogota 111711, Colombia"},{"name":"Center of Expertise in Mycology Radboudumc\/CWZ, 6500 HB Nijmegen, The Netherlands"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,1]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Cort\u00e9s, J.A., Valderrama-Rios, M.C., Pe\u00e7anha-Pietrobom, P.M., J\u00fanior, M.S., Diaz-Brochero, C., Robles-Torres, R.R., Espinosa-Almanza, C.J., Nocua-B\u00e1ez, L.C., Nucci, M., and \u00c1lvarez-Moreno, C.A. 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Lancet Microbe, 7.","DOI":"10.1016\/j.lanmic.2025.101307"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/8\/567\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:17:47Z","timestamp":1785759467000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/8\/567"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,1]]},"references-count":27,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["jof12080567"],"URL":"https:\/\/doi.org\/10.3390\/jof12080567","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,1]]}}}