{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T12:53:14Z","timestamp":1785761594764,"version":"3.56.0"},"reference-count":73,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T00:00:00Z","timestamp":1785715200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100012166","name":"National Key R&D Program of China","doi-asserted-by":"publisher","award":["2023YFD1301603"],"award-info":[{"award-number":["2023YFD1301603"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Pioneering Science and Technology Project of the National Center of Technology Innovation for Pigs","award":["NCTIP-XD\/B15"],"award-info":[{"award-number":["NCTIP-XD\/B15"]}]},{"name":"Chongqing Special Financial Fund Project","award":["25503C"],"award-info":[{"award-number":["25503C"]}]},{"name":"Chongqing Modern Agricultural Industry Technology System","award":["CQMAITS202312"],"award-info":[{"award-number":["CQMAITS202312"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Fungi","JoF"],"abstract":"<jats:p>Chinese distillers\u2019 grains (CDGs) are a lignocellulose-rich agricultural byproduct with considerable bioconversion potential; however, their recalcitrant structure imposes a major bottleneck for efficient valorization. In this study, six white-rot fungal strains and two Trichoderma strains were isolated from wild macrofungi, among which Ganoderma sp. 2G1-6 exhibited the highest laccase activity, reaching 11.29 U\/mL. Whole-genome sequencing revealed a 49.15 Mb genome containing 12,437 predicted proteins, including a substantial repertoire of 196 glycoside hydrolases and 113 auxiliary activity enzymes implicated in lignocellulose degradation. Solid-state fermentation of CDGs with Ganoderma sp. 2G1-6 produced high enzyme activities, including endoglucanase (13.3 \u00b1 4.6 U\/g), xylanase (538.8 \u00b1 33.0 U\/g), \u03b2-glucosidase (26,572 \u00b1 2996 U\/g), and laccase (231.2 \u00b1 16.7 U\/g). Concomitantly, the nutritional profile of CDGs was markedly improved after 30 days, with crude protein increasing by 28.51%, acid-soluble protein by 33.33%, and soluble dietary fiber by 159%. Complementary structural analyses (FTIR, SEM, XRD, and TGA) confirmed progressive disruption of the lignocellulosic matrix, selective delignification, and enhanced cellulose exposure. These findings establish Ganoderma sp. 2G1-6 as a promising microbial chassis for CDGs valorization and provide preliminary functional profiles of Ganoderma species for lignocellulose bioconversion.<\/jats:p>","DOI":"10.3390\/jof12080574","type":"journal-article","created":{"date-parts":[[2026,8,3]],"date-time":"2026-08-03T11:50:21Z","timestamp":1785757821000},"page":"574","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Screening White-Rot Fungi and Characterizing Lignocellulose Degradation by Ganoderma sp. from Chinese Distillers\u2019 Grains"],"prefix":"10.3390","volume":"12","author":[{"given":"Yanling","family":"Hu","sequence":"first","affiliation":[{"name":"Chongqing Academy of Animal Science, Chongqing 402460, China"},{"name":"National Center of Technology Innovation for Pigs, Chongqing 402460, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lingzhen","family":"Zhou","sequence":"additional","affiliation":[{"name":"Chongqing Academy of Animal Science, Chongqing 402460, China"},{"name":"National Center of Technology Innovation for Pigs, Chongqing 402460, China"},{"name":"College of Animal Science and Technology, Southwest University, Chongqing 402460, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ya","family":"Wang","sequence":"additional","affiliation":[{"name":"Chongqing Academy of Animal Science, Chongqing 402460, China"},{"name":"National Center of Technology Innovation for Pigs, Chongqing 402460, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0003-5516-669X","authenticated-orcid":false,"given":"Yuyin","family":"Cai","sequence":"additional","affiliation":[{"name":"Chongqing Academy of Animal Science, Chongqing 402460, China"},{"name":"National Center of Technology Innovation for Pigs, Chongqing 402460, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaofeng","family":"Guan","sequence":"additional","affiliation":[{"name":"Chongqing Academy of Animal Science, Chongqing 402460, China"},{"name":"National Center of Technology Innovation for Pigs, Chongqing 402460, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Feiyun","family":"Yang","sequence":"additional","affiliation":[{"name":"Chongqing Academy of Animal Science, Chongqing 402460, China"},{"name":"National Center of Technology Innovation for Pigs, Chongqing 402460, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zuohua","family":"Liu","sequence":"additional","affiliation":[{"name":"Chongqing Academy of Animal Science, Chongqing 402460, China"},{"name":"National Center of Technology Innovation for Pigs, Chongqing 402460, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chengling","family":"Bao","sequence":"additional","affiliation":[{"name":"Chongqing Academy of Animal Science, Chongqing 402460, China"},{"name":"National Center of Technology Innovation for Pigs, Chongqing 402460, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,3]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"221","DOI":"10.1146\/annurev-animal-030117-014838","article-title":"Meeting Global Feed Protein Demand: Challenge, Opportunity, and Strategy","volume":"7","author":"Kim","year":"2019","journal-title":"Annu. 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