{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T09:31:48Z","timestamp":1786699908745,"version":"build-2736575974"},"reference-count":41,"publisher":"MDPI AG","issue":"8","license":[{"start":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T00:00:00Z","timestamp":1786665600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Yan\u2019an University","award":["YAU202607971"],"award-info":[{"award-number":["YAU202607971"]}]},{"award":["YAU202607971"],"award-info":[{"award-number":["YAU202607971"]}],"id":[{"id":"https:\/\/ror.org\/01dyr7034","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Shaanxi Qinchuangyuan","award":["2025QCY-KXJ-128"],"award-info":[{"award-number":["2025QCY-KXJ-128"]}]},{"name":"Key Research and Development Program of Shaanxi Province","award":["2025NC-YBXM-078"],"award-info":[{"award-number":["2025NC-YBXM-078"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Fungi","JoF"],"abstract":"<jats:p>Fusarium graminearum, the major pathogen causing maize ear rot, severely threatens food security. Targeted gene deletion was used to characterize physiological functions of FgSRE1 in this pathogen. Transcriptomic profiling was conducted on axenic wild-type and \u0394FgSRE1 cultures, plus maize ear tissues colonized by both strains. Nine core DEGs functionally annotated to transmembrane transport, membrane homeostasis and oxidative stress pathways were selected for qRT-PCR validation, including four upregulated genes encoding putative transferases and five downregulated transporter genes. Phenotypic assays revealed that the \u0394FgSRE1 mutant displayed severe defects in conidial and ascospore production, along with drastically weakened pathogenicity on maize ears and leaves versus wild-type and complemented strains. qRT-PCR analysis confirmed consistent expression trends with the RNA-seq data: four putative transferase-encoding genes were up-regulated, whereas five genes related to membrane and redox transport were repressed. Together, the genetic and phenotypic results demonstrate that FgSRE1 contributes to reproductive development and virulence in F. graminearum, whereas the transcriptomic data suggest potential associations with metabolic, redox, and membrane-transport-related pathways. Our findings provide a basis for investigating the FgSRE1-associated virulence network and developing eco-friendly maize ear rot control strategies.<\/jats:p>","DOI":"10.3390\/jof12080607","type":"journal-article","created":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:08:29Z","timestamp":1786694909000},"page":"607","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Deletion of FgSRE1 Impairs Reproduction and Virulence of Fusarium graminearum and Alters Metabolic and Membrane-Transport Gene Expression"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-4545-7575","authenticated-orcid":false,"given":"Linru","family":"Shen","sequence":"first","affiliation":[{"name":"College of Life Sciences, Yan\u2019an University, Yan\u2019an 716000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongyan","family":"Hui","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Yan\u2019an University, Yan\u2019an 716000, China"},{"name":"Research and Development Centre of Ecological and Sustainable Application of Microbial Industry of the Loess Plateau in Shaanxi Province, Yan\u2019an 716000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lei","family":"Guo","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Yan\u2019an University, Yan\u2019an 716000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junshen","family":"Wang","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Yan\u2019an University, Yan\u2019an 716000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0009-6855-6850","authenticated-orcid":false,"given":"Jianchao","family":"Cui","sequence":"additional","affiliation":[{"name":"Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0004-4045-686X","authenticated-orcid":false,"given":"Haijiao","family":"Xu","sequence":"additional","affiliation":[{"name":"Changli Institute of Pomology, Hebei Academy of Agricultural and Forestry Sciences, Changli 066600, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaolong","family":"He","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Yan\u2019an University, Yan\u2019an 716000, China"},{"name":"Research and Development Centre of Ecological and Sustainable Application of Microbial Industry of the Loess Plateau in Shaanxi Province, Yan\u2019an 716000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yufei","family":"Huang","sequence":"additional","affiliation":[{"name":"College of Plant Protection, Shenyang Agricultural University, Shenyang 110866, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bo","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Life Sciences, Yan\u2019an University, Yan\u2019an 716000, China"},{"name":"Research and Development Centre of Ecological and Sustainable Application of Microbial Industry of the Loess Plateau in Shaanxi Province, Yan\u2019an 716000, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,8,14]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Ashiq, S., Edwards, S., Watson, A., and Back, M. (2022). Biofumigation for the Management of Fusarium graminearum in a Wheat-Maize Rotation. Pathogens, 11.","DOI":"10.3390\/pathogens11121427"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Magarini, A., Passera, A., Ghidoli, M., Casati, P., and Pilu, R. (2023). Genetics and Environmental Factors Associated with Resistance to Fusarium graminearum, the Causal Agent of Gibberella Ear Rot in Maize. Agronomy, 13.","DOI":"10.3390\/agronomy13071836"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1584","DOI":"10.1038\/s42003-024-07265-4","article-title":"The transcription factor FgSfp1 orchestrates mycotoxin deoxynivalenol biosynthesis in Fusarium graminearum","volume":"7","author":"Sun","year":"2024","journal-title":"Commun. Biol."},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Zhang, J., Shi, H., Yang, Y., Zeng, C., Jia, Z., Ma, T., Wu, M., Du, J., Huang, N., and Pan, G. (2023). Kernel Bioassay Evaluation of Maize Ear Rot and Genome-Wide Association Analysis for Identifying Genetic Loci Associated with Resistance to Fusarium graminearum Infection. J. Fungi, 9.","DOI":"10.20944\/preprints202310.1379.v1"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"2155","DOI":"10.3724\/SP.J.1006.2022.13055","article-title":"Precise characterization and analysis of maize germplasm resources for resistance to Fusarium ear rot and Gibberella ear rot","volume":"48","author":"Duan","year":"2022","journal-title":"Acta Agron. Sin."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Ma, Z., Wang, J., Wen, S., Ren, J., Hui, H., Huang, Y., Yang, J., Zhao, B., Liu, B., and Gao, Z. (2024). Evaluation of Maize Hybrids for Resistance to Ear Rot Caused by Dominant Fusarium Species in Northeast China. Agronomy, 14.","DOI":"10.3390\/agronomy14040855"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"821","DOI":"10.1007\/s42360-022-00515-7","article-title":"Chemical control of post-flowering stalk rot of maize with new fungicide formulations","volume":"75","author":"Gogoi","year":"2022","journal-title":"Indian Phytopathol."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Mesterhazy, A. (2024). Food Safety Aspects of Breeding Maize to Multi-Resistance against the Major (Fusarium graminearum, F. verticillioides, Aspergillus flavus) and Minor Toxigenic Fungi (Fusarium spp.) as Well as to Toxin Accumulation, Trends, and Solutions\u2014A Review. J. Fungi, 10.","DOI":"10.3390\/jof10010040"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"188","DOI":"10.1016\/j.ijbiomac.2022.02.133","article-title":"Prediction of effector proteins and their implications in pathogenicity of phytopathogenic filamentous fungi: A review","volume":"206","author":"Wu","year":"2022","journal-title":"Int. J. Biol. Macromol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"927","DOI":"10.1126\/science.adw1568","article-title":"Outsmarted by fungi","volume":"387","author":"Gutjahr","year":"2025","journal-title":"Science"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"1460","DOI":"10.1111\/nph.70075","article-title":"Structure-guided insights into the biology of fungal effectors","volume":"246","author":"Lahfa","year":"2025","journal-title":"New Phytol."},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Gu, X., Cao, Z., Li, Z., Yu, H., and Liu, W. (2024). Plant immunity suppression by an \u03b2-1,3-glucanase of the maize anthracnose pathogen Colletotrichum graminicola. BMC Plant Biol., 24.","DOI":"10.1186\/s12870-024-05053-0"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"618","DOI":"10.1038\/s41477-024-01642-x","article-title":"Targeting Magnaporthe oryzae effector MoErs1 and host papain-like protease OsRD21 interaction to combat rice blast","volume":"10","author":"Liu","year":"2024","journal-title":"Nat. Plants"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Yang, G., Yang, J., Zhang, Q., Wang, W., Feng, L., Zhao, L., An, B., Wang, Q., He, C., and Luo, H. (2022). The effector protein CgNLP1 of Colletotrichum gloeosporioides affects invasion and disrupts nuclear localization of necrosis-induced transcription factor HbMYB8-Like to suppress plant defense signaling. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.911479"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"297","DOI":"10.1094\/MPMI-08-24-0103-FI","article-title":"The Fusarium graminearum effector protease FgTPP1 suppresses immune responses and facilitates Fusarium head blight disease","volume":"38","author":"Darino","year":"2025","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"2131","DOI":"10.1094\/PHYTO-02-24-0068-R","article-title":"Effector Protein Serine Carboxypeptidase FgSCP Is Essential for Full Virulence in Fusarium graminearum and Is Involved in Modulating Plant Immune Responses","volume":"114","author":"Liu","year":"2024","journal-title":"Phytopathology"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"eadw6401","DOI":"10.1126\/sciadv.adw6401","article-title":"Plasticity of the cytotoxic Nep1-like protein enables promiscuity in binding to its lipid receptor glycosylinositol phosphorylceramides","volume":"11","author":"Kalanj","year":"2025","journal-title":"Sci. Adv."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4686","DOI":"10.1038\/ncomms5686","article-title":"Unconventionally secreted effectors of two filamentous pathogens target plant salicylate biosynthesis","volume":"5","author":"Liu","year":"2014","journal-title":"Nat. Commun."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"He, S., Huang, Y., Sun, Y., Liu, B., Wang, S., Xuan, Y., and Gao, Z. (2022). The secreted ribonuclease SRE1 contributes to Setosphaeria turcica virulence and activates plant immunity. Front. Microbiol., 13.","DOI":"10.3389\/fmicb.2022.941991"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"365","DOI":"10.1111\/jipb.12997","article-title":"Fg12 ribonuclease secretion contributes to Fusarium graminearum virulence and induces plant cell death","volume":"63","author":"Yang","year":"2020","journal-title":"J. Integr. Plant Biol."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Hao, Z., Pan, L., Xu, J., Yu, C., Li, J., and Luo, L. (2025). FGSE02, a Novel Secreted Protein in Fusarium graminearum FG-12, Leads to Cell Death in Plant Tissues and Modulates Fungal Virulence. J. Fungi, 11.","DOI":"10.3390\/jof11050397"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"440","DOI":"10.1093\/bfgp\/elu033","article-title":"Transcriptome characteristics of filamentous fungi deduced using high-throughput analytical technologies","volume":"13","author":"Meijueiro","year":"2014","journal-title":"Brief. Funct. Genom."},{"key":"ref_23","first-page":"1","article-title":"Using RNA-seq for analysis of differential gene expression in fungal species","volume":"Volume 1361","author":"Devaux","year":"2016","journal-title":"Yeast Functional Genomics: Methods and Protocols"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"e03024-19","DOI":"10.1128\/AEM.03024-19","article-title":"FgHtf1 Regulates Global Gene Expression towards Aerial Mycelium and Conidiophore Formation in the Cereal Fungal Pathogen Fusarium graminearum","volume":"86","author":"Fan","year":"2020","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"51","DOI":"10.1007\/s00253-009-2416-7","article-title":"New tools for the genetic manipulation of filamentous fungi","volume":"86","author":"Hoff","year":"2010","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"4237","DOI":"10.1021\/acs.jafc.3c08093","article-title":"Characterization of SsHog1 and Shk1 Using Efficient Gene Knockout Systems through Repeated Protoplasting and CRISPR\/Cas9 Ribonucleoprotein Approaches in Sclerotinia sclerotiorum","volume":"72","author":"Ma","year":"2024","journal-title":"J. Agric. Food Chem."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"7879","DOI":"10.1007\/s00253-020-10717-6","article-title":"FvatfA regulates growth, stress tolerance as well as mycotoxin and pigment productions in Fusarium verticillioides","volume":"104","author":"Murvai","year":"2020","journal-title":"Appl. Microbiol. Biotechnol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"33","DOI":"10.1186\/s42483-020-00074-7","article-title":"FpCzf14 is a putative C2H2 transcription factor regulating conidiation in Fusarium pseudograminearum","volume":"2","author":"Chen","year":"2020","journal-title":"Phytopathol. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"357","DOI":"10.1038\/nmeth.3317","article-title":"HISAT: A fast spliced aligner with low memory requirements","volume":"12","author":"Kim","year":"2015","journal-title":"Nat. Methods"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"550","DOI":"10.1186\/s13059-014-0550-8","article-title":"Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2","volume":"15","author":"Love","year":"2014","journal-title":"Genome Biol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"543","DOI":"10.1007\/s10658-024-02850-z","article-title":"Dynamics of Fusarium graminearum inoculum on residues of naturally infected winter and summer crops","volume":"169","author":"Mourelos","year":"2024","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Buttar, Z.A., Cheng, M., Wei, P., Zhang, Z., Lv, C., Zhu, C., Ali, N.F., Kang, G., Wang, D., and Zhang, K. (2024). Update on the basic understanding of Fusarium graminearum virulence factors in common wheat research. Plants, 13.","DOI":"10.3390\/plants13081159"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"127506","DOI":"10.1016\/j.micres.2023.127506","article-title":"Virulence factors of the genus Fusarium with targets in plants","volume":"277","author":"Plasencia","year":"2023","journal-title":"Microbiol. Res."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"e70174","DOI":"10.1111\/mpp.70174","article-title":"FgNdk1 Promotes Effector Secretion to Scavenge ROS During Fusarium graminearum Infection","volume":"26","author":"Sun","year":"2025","journal-title":"Mol. Plant Pathol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"478","DOI":"10.1094\/MPMI-12-22-0254-R","article-title":"Fusarium graminearum Effector FgNls1 Targets Plant Nuclei to Induce Wheat Head Blight","volume":"36","author":"Hao","year":"2023","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_36","doi-asserted-by":"crossref","unstructured":"Alouane, T., Rimbert, H., Bormann, J., Gonz\u00e1lez-Montiel, G.A., Loesgen, S., Sch\u00e4fer, W., Freitag, M., Langin, T., and Bonhomme, L. (2021). Comparative Genomics of Eight Fusarium graminearum Strains with Contrasting Aggressiveness Reveals an Expanded Open Pangenome and Extended Effector Content Signatures. Int. J. Mol. Sci., 22.","DOI":"10.3390\/ijms22126257"},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Garcia-Ceron, D., Lowe, R.G.T., McKenna, J.A., Brain, L.M., Dawson, C.S., Clark, B., Berkowitz, O., Faou, P., Whelan, J., and Bleackley, M.R. (2021). Extracellular Vesicles from Fusarium graminearum Contain Protein Effectors Expressed during Infection of Corn. J. Fungi, 7.","DOI":"10.3390\/jof7110977"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"708","DOI":"10.1080\/00275514.2024.2373665","article-title":"Genome-wide search and gene expression studies reveal candidate effectors with a role in pathogenicity and virulence in Fusarium graminearum","volume":"116","author":"Fatima","year":"2024","journal-title":"Mycologia"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Li, P., Zhao, R., Fang, Y., Fan, Y., Hu, Q., Huang, W., Ma, W., and Zhang, C. (2025). Proteomic Analysis of the Fusarium graminearum Secretory Proteins in Wheat Apoplast Reveals a Cell-Death-Inducing M43 Peptidase. J. Fungi, 11.","DOI":"10.3390\/jof11040240"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1094\/MPMI-03-25-0027-R","article-title":"An RGAE homolog in Fusarium graminearum is critical for initial infection in wheat and barley","volume":"38","author":"Rhoades","year":"2025","journal-title":"Mol. Plant-Microbe Interact."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"369","DOI":"10.1111\/tpj.15949","article-title":"Proximity-dependent biotinylation identifies a suite of candidate effector proteins from Fusarium graminearum","volume":"112","author":"Miltenburg","year":"2022","journal-title":"Plant J."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/8\/607\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,14]],"date-time":"2026-08-14T08:47:54Z","timestamp":1786697274000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/8\/607"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,14]]},"references-count":41,"journal-issue":{"issue":"8","published-online":{"date-parts":[[2026,8]]}},"alternative-id":["jof12080607"],"URL":"https:\/\/doi.org\/10.3390\/jof12080607","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,14]]}}}