{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:32:34Z","timestamp":1787923954797,"version":"build-2784847793"},"reference-count":45,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T00:00:00Z","timestamp":1787702400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Qingyang City Science and Technology Planning Project","award":["QY-STK-2024A-089"],"award-info":[{"award-number":["QY-STK-2024A-089"]}]},{"name":"Science and Technology Innovation Guidance Program of Gansu Province","award":["26CXNM001"],"award-info":[{"award-number":["26CXNM001"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Fungi","JoF"],"abstract":"<jats:p>Fusarium solani is a major soil-borne pathogen responsible for root rot diseases, posing a significant threat to agricultural productivity. The development of environmentally sustainable alternatives to chemical fungicides is therefore urgently needed. This study evaluated the antifungal activity of fermentation broths and extracellular metabolites derived from Tilletia laevis fungi at different developmental stages against F. solani under in vitro conditions. Six test agents, including carbendazim (one positive fungicide control), caffeic acid, phenylacetylglycine, and 6-hydroxypyridine-2-carboxylic acid (candidate bioactive metabolites), were evaluated for mycelial growth inhibition, EC50 values, and physiological responses. All treatments exhibited concentration-dependent inhibitory effects, with carbendazim showing the highest activity (EC50 = 21.26 mg L\u22121). Among the metabolites, 6-hydroxypyridine-2-carboxylic acid and phenylacetylglycine demonstrated notable antifungal efficacy, particularly at higher concentrations. Fermentation broths from the promycelial stage showed stronger inhibition than those from the teliospore stage, indicating stage-specific metabolite activity. Biochemical analyses of F. solani mycelia revealed significant changes in soluble protein, soluble sugar, and antioxidant enzyme activities (SOD and POD), suggesting that antifungal effects are mediated through metabolic disruption and oxidative stress. These findings highlight the potential of T. laevis-derived metabolites as eco-friendly biofungicides and provide a theoretical basis for sustainable management of soil-borne diseases. Further studies are required to identify active compounds and validate their efficacy under field conditions.<\/jats:p>","DOI":"10.3390\/jof12090639","type":"journal-article","created":{"date-parts":[[2026,8,26]],"date-time":"2026-08-26T15:04:32Z","timestamp":1787756672000},"page":"639","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Metabolites of Tilletia laevis Suppress Fusarium solani Growth Through Metabolic Disruption and Oxidative Stress Responses"],"prefix":"10.3390","volume":"12","author":[{"given":"Delai","family":"Chen","sequence":"first","affiliation":[{"name":"College of Agriculture and Bioengineering, Longdong University, Qingyang 745000, China"},{"name":"Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration, Qingyang 745000, 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China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"}]},{"given":"Liting","family":"Chen","sequence":"additional","affiliation":[{"name":"College of Agriculture and Bioengineering, Longdong University, Qingyang 745000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"}]},{"given":"Pin","family":"Liu","sequence":"additional","affiliation":[{"name":"College of Agriculture and Bioengineering, Longdong University, Qingyang 745000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"resources","vocabulary":"credit"}]},{"given":"Duo","family":"Jin","sequence":"additional","affiliation":[{"name":"College of Agriculture and Bioengineering, Longdong University, Qingyang 745000, 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(2022). Metabolomic analysis of wheat grains after Tilletia laevis K\u00fchn infection by using ultrahigh-performance liquid chromatography\u2013Q-exactive mass spectrometry. Metabolites, 12.","DOI":"10.3390\/metabo12090805"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"111153","DOI":"10.1016\/j.ygeno.2025.111153","article-title":"Whole genome sequencing of Tilletia laevis, the fungal pathogen causing common bunt","volume":"173","author":"Yang","year":"2025","journal-title":"Genomics"},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"He, T., Ren, Z., Muhae-Ud-Din, G., Guo, Q., Liu, T., Chen, W., and Gao, L. (2022). Transcriptomics analysis of wheat tassel response to Tilletia laevis K\u00fchn, which causes common bunt of wheat. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.823907"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Lunzer, M., Dumalasov\u00e1, V., Pfatrisch, K., Buerstmayr, H., and Grausgruber, H. (2023). Common bunt in organic wheat: Unravelling infection characteristics relevant for resistance breeding. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1264458"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Zhang, C., Chen, W., Wang, B., Wang, Y., Li, N., Li, R., Yan, Y., Sun, Y., and He, J. (2024). Potato glycoside alkaloids exhibit antifungal activity by regulating the tricarboxylic acid cycle pathway of Fusarium solani. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1390269"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"527","DOI":"10.1080\/07060661.2020.1745893","article-title":"Effect of temperature on Fusarium solani and F. tricinctum growth and disease development in soybean","volume":"42","author":"Yan","year":"2020","journal-title":"Can. J. Plant Pathol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"2974","DOI":"10.1094\/PDIS-12-21-2738-RE","article-title":"Effects of soil type, temperature, and moisture on development of Fusarium root rot of soybean by Fusarium solani (FSSC 11) and Fusarium tricinctum","volume":"106","author":"Yan","year":"2022","journal-title":"Plant Dis."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1007\/s42360-019-00151-8","article-title":"Carbendazim sensitivity of Fusarium solani soil isolates","volume":"72","author":"Tarafder","year":"2019","journal-title":"Indian Phytopathol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"100410","DOI":"10.1016\/j.emcon.2024.100410","article-title":"A comprehensive review on environmental and human health impacts of chemical pesticide usage","volume":"11","author":"Zhou","year":"2025","journal-title":"Emerg. Contam."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Leskovac, A., and Petrovi\u0107, S. (2023). Pesticide use and degradation strategies: Food safety, challenges and perspectives. Foods, 12.","DOI":"10.3390\/foods12142709"},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Attia, M.S., Abdelaziz, A.M., Al-Askar, A.A., Arishi, A.A., Abdelhakim, A.M., and Hashem, A.H. (2022). Plant growth-promoting fungi as biocontrol tool against fusarium wilt disease of tomato plant. J. Fungi, 8.","DOI":"10.3390\/jof8080775"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Torres-Rodriguez, J.A., Reyes-P\u00e9rez, J.J., Carranza-Pati\u00f1o, M.S., Herrera-Feijoo, R.J., Preciado-Rangel, P., and Hernandez-Montiel, L.G. (2025). Biocontrol of Fusarium solani: Antifungal activity of chitosan and induction of defence enzymes. Plants, 14.","DOI":"10.3390\/plants14030431"},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Xu, D., Xue, M., Shen, Z., Jia, X., Hou, X., Lai, D., and Zhou, L. (2021). Phytotoxic secondary metabolites from fungi. Toxins, 13.","DOI":"10.3390\/toxins13040261"},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Jabran, M., Shafique, M.S., Abbas, A., Han, W., Ali, M.A., and Gao, L. (2025). Antifungal potential of silver and copper nanoparticles in suppressing Tilletia indica for Karnal bunt resistance in wheat. BMC Plant Biol., 25.","DOI":"10.1186\/s12870-025-07547-x"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Prabhu, S., Poorniammal, R., and Dufoss\u00e9, L. (2025). Microbial metabolites: A sustainable approach to combat plant pests. Metabolites, 15.","DOI":"10.3390\/metabo15060418"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"4987","DOI":"10.1007\/s00344-026-12193-0","article-title":"Transcriptional Regulation and Functional Dynamics of Secondary Metabolites in Plant Adaptation and Defense","volume":"45","author":"Ayub","year":"2026","journal-title":"J. Plant Growth Regul."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"154","DOI":"10.9734\/ajrcs\/2024\/v9i1257","article-title":"Management of Fusarium wilt of tomato (Fusarium oxysporum f. sp. lycopersici) and related soil-borne diseases using eco-friendly methods: A review","volume":"9","author":"Haruna","year":"2024","journal-title":"Asian J. Res. Crop Sci."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"4186","DOI":"10.1002\/ps.8122","article-title":"Rhizospheric microbiota of suppressive soil protect plants against Fusarium solani infection","volume":"80","author":"Li","year":"2024","journal-title":"Pest Manag. Sci."},{"key":"ref_19","doi-asserted-by":"crossref","unstructured":"Baroja-Fern\u00e1ndez, E., Bahaji, A., and Mu\u00f1oz, F.J. (2025). Fungal Phytopathogens as a Source of Metabolites for Agricultural Applications. Fungal Metabolites for Agricultural Applications: Biostimulation and Crop Protection by Fungal Biotechnology, Springer Nature Switzerland.","DOI":"10.1007\/978-3-031-76587-2_13"},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Amuzu, P., Pan, X., Hou, X., Sun, J., Jakada, M.A., Odigie, E., Xu, D., Lai, D., and Zhou, L. (2024). Recent updates on the secondary metabolites from Fusarium fungi and their biological activities covering 2019 to 2024. J. Fungi, 10.","DOI":"10.3390\/jof10110778"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"2613","DOI":"10.1080\/87559129.2025.2487893","article-title":"Microbial-Derived Bioactive Substances: Sources, Identification, and Application in Fruit Disease Management","volume":"41","author":"Yang","year":"2025","journal-title":"Food Rev. Int."},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Abo-Elyousr, K.A., Ibrahim, O.H., Al-Qurashi, A.D., Mousa, M.A., and Saad, M.M. (2022). Biocontrol potential of endophytic fungi for the eco-friendly management of root rot of Cuminum cyminum caused by Fusarium solani. Agronomy, 12.","DOI":"10.3390\/agronomy12112612"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"850","DOI":"10.1038\/159850b0","article-title":"Distortion of fungal hyphae in the presence of certain inhibitors","volume":"159","author":"Vincent","year":"1947","journal-title":"Nature"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"113380","DOI":"10.1016\/j.scienta.2024.113380","article-title":"In vitro control of Fusarium oxysporum f. sp. lycopersici Race 3 by the innovative formulation consisting of a thymol-based emulsion, and evaluation of its effects on the vigor of tomato seeds","volume":"336","author":"Peccini","year":"2024","journal-title":"Sci. Hortic."},{"key":"ref_25","first-page":"e242502","article-title":"Plant extracts inhibiting mycelial growth of Fusarium solani f. sp. piperis and Phytopythium helicoides","volume":"50","author":"Fernandes","year":"2024","journal-title":"Summa Phytopathol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"417","DOI":"10.1007\/s42994-024-00181-5","article-title":"Thiophanate-methyl and its major metabolite carbendazim weaken rhizobacteria-mediated defense responses in cucumbers against Fusarium wilt","volume":"5","author":"Cui","year":"2024","journal-title":"Abiotech"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"e26383","DOI":"10.1016\/j.heliyon.2024.e26718","article-title":"Salt stress influences the proliferation of Fusarium solani and enhances the severity of wilt disease in potato","volume":"10","author":"Tiwari","year":"2024","journal-title":"Heliyon"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"91","DOI":"10.1007\/s11240-023-02446-1","article-title":"Developing resistance against soil-borne Fusarium pathogen causing tomato wilt through in vitro cell line selection","volume":"153","author":"Shaunak","year":"2023","journal-title":"Plant Cell Tissue Organ Cult."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"18137","DOI":"10.1007\/s11356-015-5005-6","article-title":"Antifungal efficiency of a lipopeptide biosurfactant derived from Bacillus subtilis SPB1 versus the phytopathogenic fungus, Fusarium solani","volume":"22","author":"Mnif","year":"2015","journal-title":"Environ. Sci. Pollut. Res."},{"key":"ref_30","doi-asserted-by":"crossref","unstructured":"Su, L., Zhang, L., Nie, D., Kuramae, E.E., Shen, B., and Shen, Q. (2020). Bacterial tomato pathogen Ralstonia solanacearum invasion modulates rhizosphere compounds and facilitates the cascade effect of fungal pathogen Fusarium solani. Microorganisms, 8.","DOI":"10.3390\/microorganisms8060806"},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"967","DOI":"10.1111\/jph.12517","article-title":"Application of Soil-borne Actinomycetes for Biological Control against Fusarium Wilt of Chickpea (Cicer arietinum) caused by Fusarium solani fsp pisi","volume":"164","author":"Soltanzadeh","year":"2016","journal-title":"J. Phytopathol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"279","DOI":"10.3390\/bacteria1040021","article-title":"Isolation and Characterization of Bacillus velezensis from Lake Bogoria as a Potential Biocontrol of Fusarium solani in Phaseolus vulgaris L.","volume":"1","author":"Wekesa","year":"2022","journal-title":"Bacteria"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1186\/s41938-019-0145-5","article-title":"Isolation and molecular identification of Rhizoctonia solani and Fusarium solani isolated from cucumber (Cucumis sativus L.) and their control feasibility by Pseudomonas fluorescens and Bacillus subtilis","volume":"29","author":"Alkhafije","year":"2019","journal-title":"Egypt. J. Biol. Pest Control"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"126440","DOI":"10.1016\/j.micres.2020.126440","article-title":"Forest tree associated bacteria for potential biological control of Fusarium solani and of Fusarium kuroshium, causal agent of Fusarium dieback","volume":"235","author":"Reverchon","year":"2020","journal-title":"Microbiol. Res."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"13562","DOI":"10.15835\/nbha52213562","article-title":"Marine actinomycetes for biocontrol of Fusarium solani in tomato plants: In vitro and in vivo studies","volume":"52","author":"Adame","year":"2024","journal-title":"Not. Bot. Horti Agrobot. Cluj-Napoca"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3532","DOI":"10.1002\/ps.70474","article-title":"Control of tobacco Fusarium root rot by Bacillus amyloliquefaciens HN11 and its influence on the rhizosphere microbial community","volume":"82","author":"Zhang","year":"2026","journal-title":"Pest Manag. Sci."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"104774","DOI":"10.1016\/j.biocontrol.2021.104774","article-title":"Biocontrol mechanisms of Trichoderma harzianum ITEM 3636 against peanut brown root rot caused by Fusarium solani RC 386","volume":"164","author":"Erazo","year":"2021","journal-title":"Biol. Control"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1007\/s10658-016-1104-6","article-title":"Biochemical and plant nutrient alterations induced by Meloidogyne javanica and Fusarium oxysporum f. sp. radicis lycopersici co-infection on tomato cultivars with differing level of resistance to M. javanica","volume":"148","author":"Lobna","year":"2017","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_39","first-page":"227","article-title":"Differential physiological and molecular responses of susceptible and resistant tomato genotypes to Alternaria solani infection","volume":"12","author":"Haghpanah","year":"2023","journal-title":"J. Crop Prot."},{"key":"ref_40","first-page":"155","article-title":"Biochemical Analysis of Healthy and Infected Soybean Plants with Fusarium solani and Fusarium oxysporum","volume":"2","author":"Waghmode","year":"2025","journal-title":"Sciences"},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"120963","DOI":"10.1016\/j.indcrop.2025.120963","article-title":"Biocontrol effects and underlying mechanism of Bacillus subtilis Pn1 on Panax notoginseng root rot caused by Fusarium solani","volume":"229","author":"Kunfa","year":"2025","journal-title":"Ind. Crops Prod."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"e14435","DOI":"10.1111\/1751-7915.14435","article-title":"Streptomyces-triggered coordination between rhizosphere microbiomes and plant transcriptome enables watermelon Fusarium wilt resistance","volume":"17","author":"Ge","year":"2024","journal-title":"Microb. Biotechnol."},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1007\/s11104-025-08010-4","article-title":"Chemical dialogues in the rhizosphere: Metabolomics perspectives on plant defence and microbial interactions","volume":"518","author":"Sibanyoni","year":"2026","journal-title":"Plant Soil"},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Chen, Q., Song, Y., An, Y., Lu, Y., and Zhong, G. (2024). Mechanisms and impact of rhizosphere microbial metabolites on crop health, traits, functional components: A comprehensive review. Molecules, 29.","DOI":"10.3390\/molecules29245922"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"11103","DOI":"10.1038\/s41598-023-37837-z","article-title":"Biocontrol agent of root-knot nematode Meloidogyne javanica and root-rot fungi, Fusarium solani in okra morphological, anatomical characteristics and productivity under greenhouse conditions","volume":"13","author":"Ali","year":"2023","journal-title":"Sci. Rep."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/9\/639\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,28]],"date-time":"2026-08-28T13:02:53Z","timestamp":1787922173000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/9\/639"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,8,26]]},"references-count":45,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["jof12090639"],"URL":"https:\/\/doi.org\/10.3390\/jof12090639","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,8,26]]}}}