{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T13:12:00Z","timestamp":1788786720034,"version":"build-2803163510"},"reference-count":53,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,6]],"date-time":"2026-09-06T00:00:00Z","timestamp":1788652800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32160063"],"award-info":[{"award-number":["32160063"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32160063"],"award-info":[{"award-number":["32160063"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"the Science Research Fund Project of the Yunnan Provinc","award":["2026J1148"],"award-info":[{"award-number":["2026J1148"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Fungi","JoF"],"abstract":"<jats:p>Wild Polygala crotalarioides is an ethnomedicinal plant traditionally used for neurological health. Endophytic fungi associated with medicinal plants are increasingly recognized as sources of bioactive natural products; however, their diversity and biological activities in wild P. crotalarioides remain poorly understood. We used high-throughput sequencing and culture-dependent methods to explored the diversity, community composition, and bioactivities of endophytic fungi associated with wild P. crotalarioides. Culturable isolates were identified via ITS sequencing and further screened for AChE-inhibitory and antioxidant activities. Our results revealed that a total of 818 fungal OTUs were detected, revealing pronounced tissue-specific community differentiation. Stem tissues harbored the highest fungal diversity, richness, and number of unique OTUs. Ascomycota and Basidiomycota dominated fungal communities across all tissues, while distinct biomarker taxa were enriched in different organs. Functional annotation indicated marked tissue-dependent variation in trophic composition. Among 108 culturable isolates, Penicillium sp. PRP024 and Trichoderma sp. PRP008 exhibited the strongest AChE inhibitory activities (&gt;80%), whereas Fusarium sp. PSP053, Diaporthe sp. PSM027 and Exserohilum sp. PSP042 showed notable antioxidant activities. Wild P. crotalarioides hosts highly diverse and tissue-specific endophytic fungal communities. Several isolates exhibited promising acetylcholinesterase inhibitory and antioxidant activities, highlighting medicinal plant-associated endophytic fungi as potential sources of antioxidative natural products.<\/jats:p>","DOI":"10.3390\/jof12090669","type":"journal-article","created":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T12:12:25Z","timestamp":1788783145000},"page":"669","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Endophytic Fungi from Wild Polygala crotalarioides: Diversity, Antioxidant Capacity and Acetylcholinesterase Inhibitory Potential"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0441-4257","authenticated-orcid":false,"given":"Kaize","family":"Shen","sequence":"first","affiliation":[{"name":"Yunnan Key Laboratory of Gastrodia and Fungi Symbiotic Biology, Zhaotong University, Zhaotong 657000, China"},{"name":"Yunnan Engineering Research Center of 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Ethnopharmacol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"200","DOI":"10.1038\/s41573-020-00114-z","article-title":"Natural products in drug discovery: Advances and opportunities","volume":"20","author":"Atanasov","year":"2021","journal-title":"Nat. Rev. Drug Discov."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Roviello, G.N. (2025). Nature-Inspired Pathogen and Cancer Protein Covalent Inhibitors: From Plants and Other Natural Sources to Drug Development. Pathogens, 14.","DOI":"10.3390\/pathogens14111153"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Aleynova, O.A., and Kiselev, K.V. (2023). Interaction of plants and endophytic microorganisms: Molecular aspects, biological functions, community composition, and practical applications. Plants, 12.","DOI":"10.3390\/plants12040714"},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"108174","DOI":"10.1016\/j.plaphy.2023.108174","article-title":"Endophytic fungi: Unravelling plant-endophyte interaction and the multifaceted role of fungal endophytes in stress amelioration","volume":"206","author":"Waqar","year":"2024","journal-title":"Plant Physiol. Biochem."},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Nazir, A., Puthuveettil, A.R., Hussain, F.H.N., Hamed, K.E., and Munawar, N. (2024). Endophytic fungi: Nature\u2019s solution for antimicrobial resistance and sustainable agriculture. Front. Microbiol., 15.","DOI":"10.3389\/fmicb.2024.1461504"},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"Tsipinana, S., Husseiny, S., Alayande, K.A., Raslan, M., Amoo, S., and Adeleke, R. (2023). Contribution of endophytes towards improving plant bioactive metabolites: A rescue option against red-taping of medicinal plants. Front. Plant Sci., 14.","DOI":"10.3389\/fpls.2023.1248319"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Liu, M., Wang, X., and Gao, D. (2024). Polygalae Radix: Review of metabolites, pharmacological activities and toxicology. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1420853"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"106233","DOI":"10.1016\/j.fitote.2024.106233","article-title":"Polygala japonica Houtt.: A comprehensive review on its botany, traditional uses, phytochemistry, pharmacology, and pharmacokinetics","volume":"179","author":"Tang","year":"2024","journal-title":"Fitoterapia"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1186\/s12934-023-02118-x","article-title":"Bioactive compounds and biomedical applications of endophytic fungi: A recent review","volume":"22","author":"Hashem","year":"2023","journal-title":"Microb. Cell Fact."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhu, J., Wang, Z., Song, L., Fu, W., and Liu, L. (2023). Anti-Alzheimer\u2019s natural products derived from plant endophytic fungi. Molecules, 28.","DOI":"10.3390\/molecules28052259"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Li, S., Hou, Z., Ye, T., Song, X., Hu, X., and Chen, J. (2024). Saponin components in Polygala tenuifolia as potential candidate drugs for treating dementia. Front. Pharmacol., 15.","DOI":"10.3389\/fphar.2024.1431894"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"4621","DOI":"10.1002\/ptr.7930","article-title":"Tenuifolin in the prevention of Alzheimer\u2019s disease-like phenotypes: Investigation of the mechanisms from the perspectives of calpain system, ferroptosis, and apoptosis","volume":"37","author":"Li","year":"2023","journal-title":"Phytother. Res."},{"key":"ref_14","doi-asserted-by":"crossref","unstructured":"Grabowska, W., Bijak, M., Szelenberger, R., Gorniak, L., Podogrocki, M., Harmata, P., and Cichon, N. (2025). Acetylcholinesterase as a multifunctional target in amyloid-driven neurodegeneration: From dual-site inhibitors to anti-agregation strategies. Int. J. Mol. Sci., 26.","DOI":"10.3390\/ijms26178726"},{"key":"ref_15","first-page":"342","article-title":"The role of oxidative stress in neurodegenerative diseases and potential antioxidant therapies","volume":"3","author":"Calmarza","year":"2022","journal-title":"Adv. Lab. Med."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"1179","DOI":"10.17219\/acem\/176051","article-title":"Safety and efficacy of acetylcholinesterase inhibitors for Alzheimer\u2019s disease: A systematic review and meta-analysis","volume":"33","author":"Gao","year":"2024","journal-title":"Adv. Clin. Exp. Med."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Liu, S., Zhao, M., Liu, Y., Yang, X., Yan, H., Xu, H., Wu, Y., and Xu, Y. (2025). Comparative efficacy and safety of symptomatic therapy and disease-modifying therapy for Alzheimer\u2019s disease: A systematic review and network meta-analysis. Front. Neurosci., 19.","DOI":"10.3389\/fnins.2025.1656906"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Salgotra, R.K., and Chauhan, B.S. (2023). Genetic diversity, conservation, and utilization of plant genetic resources. Genes, 14.","DOI":"10.3390\/genes14010174"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3303","DOI":"10.1007\/s10722-023-01826-y","article-title":"Phenotypic diversity analysis of Polygala tenuifolia Willd germplasm resources in China","volume":"71","author":"Tian","year":"2024","journal-title":"Genet. Resour. Crop Evol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"2838","DOI":"10.1080\/23802359.2021.1964396","article-title":"The complete chloroplast genome characteristics of Polygala crotalarioides Buch.-Ham. ex DC. (Polygalaceae) from Yunnan, China","volume":"6","author":"Ma","year":"2021","journal-title":"Mitochondrial DNA Part B Resour."},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Shen, K., Xiong, Y., Liu, Y., Fan, X., Zhu, R., Hu, Z., Li, C., and Hua, Y. (2024). Community Structure and Diversity of Endophytic Fungi in Cultivated Polygala crotalarioides at Two Different Growth Stages Based on Culture-Independent and Culture-Based Methods. J. Fungi, 10.","DOI":"10.3390\/jof10030195"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"675","DOI":"10.1007\/s00203-022-03283-0","article-title":"Methods used for the study of endophytic fungi: A review on methodologies and challenges, and associated tips","volume":"204","author":"Lorenzi","year":"2022","journal-title":"Arch. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Zheng, H., Qiao, M., Xu, J., and Yu, Z. (2021). Culture-based and culture-independent assessments of endophytic fungal diversity in aquatic plants in southwest China. Front. Fungal Biol., 2.","DOI":"10.3389\/ffunb.2021.692549"},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Zuo, Y.L., Hu, Q.N., Qin, L., Liu, J.Q., and He, X.L. (2022). Species identity and combinations differ in their overall benefits to Astragalus adsurgens plants inoculated with single or multiple endophytic fungi under drought conditions. Front. Plant Sci., 13.","DOI":"10.3389\/fpls.2022.933738"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"i884","DOI":"10.1093\/bioinformatics\/bty560","article-title":"Fastp: An ultra-fast all-in-one FASTQ preprocessor","volume":"34","author":"Chen","year":"2018","journal-title":"Bioinformatics"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2957","DOI":"10.1093\/bioinformatics\/btr507","article-title":"FLASH: Fast length adjustment of short reads to improve genome assemblies","volume":"27","author":"Salzberg","year":"2011","journal-title":"Bioinformatics"},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"996","DOI":"10.1038\/nmeth.2604","article-title":"UPARSE: Highly accurate OTU sequences from microbial amplicon reads","volume":"10","author":"Edgar","year":"2013","journal-title":"Nat. Methods"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"5271","DOI":"10.1111\/mec.12481","article-title":"Towards a unified paradigm for sequence-based identification of fungi","volume":"22","author":"Nilsson","year":"2013","journal-title":"Mol. Ecol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"5261","DOI":"10.1128\/AEM.00062-07","article-title":"Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy","volume":"73","author":"Wang","year":"2007","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"7537","DOI":"10.1128\/AEM.01541-09","article-title":"Introducing mothur: Open-source, platform-independent, community-supported software for describing and comparing microbial communities","volume":"75","author":"Schloss","year":"2009","journal-title":"Appl. Environ. Microbiol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1016\/j.funeco.2015.06.006","article-title":"FUNGuild: An open annotation tool for parsing fungal community datasets by ecological guild","volume":"20","author":"Nguyen","year":"2016","journal-title":"Fungal Ecol."},{"key":"ref_32","doi-asserted-by":"crossref","unstructured":"Chen, H., and Boutros, P.C. (2011). VennDiagram: A package for the generation of highly-customizable Venn and Euler diagrams in R. BMC Bioinform., 12.","DOI":"10.1186\/1471-2105-12-35"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"647","DOI":"10.1002\/jobm.202100462","article-title":"Surface sterilization for isolation of endophytes: Ensuring what (not) to grow","volume":"62","author":"Sahu","year":"2022","journal-title":"J. Basic Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"617","DOI":"10.1046\/j.1469-8137.2000.00716.x","article-title":"Identification of endophytic fungi from Livistona chinensis based on morphology and rDNA sequences","volume":"147","author":"Guo","year":"2000","journal-title":"New Phytol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"16152","DOI":"10.1038\/s41598-019-52481-2","article-title":"Isolation of endophytic fungi and screening of Huperzine A-producing fungus from Huperzia serrata in Vietnam","volume":"9","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"88","DOI":"10.1016\/0006-2952(61)90145-9","article-title":"A new and rapid colorimetric determination of acetylcholinesterase activity","volume":"7","author":"Ellman","year":"1961","journal-title":"Biochem. Pharmacol."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"fnv184","DOI":"10.1093\/femsle\/fnv184","article-title":"Diversity, antimicrobial and antioxidant activities of culturable bacterial endophyte communities in Aloe vera","volume":"362","author":"Akinsanya","year":"2015","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"110623","DOI":"10.1016\/j.foodres.2021.110623","article-title":"Untargeted and targeted metabolomics reveal changes in the chemical constituents of instant dark tea during liquid-state fermentation by Eurotium cristatum","volume":"148","author":"An","year":"2021","journal-title":"Food Res. Int."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1186\/s13020-019-0241-1","article-title":"Biofertilizers regulate the soil microbial community and enhance Panax ginseng yields","volume":"14","author":"Dong","year":"2019","journal-title":"Chin. Med."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"2107","DOI":"10.1111\/1462-2920.14968","article-title":"The diversity and distribution of endophytes across biomes, plant phylogeny and host tissues: How far have we come and where do we go from here?","volume":"22","author":"Harrison","year":"2020","journal-title":"Environ. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","unstructured":"Fang, K., Miao, Y.F., Chen, L., Zhou, J., Yang, Z.P., Dong, X.F., and Zhang, H.B. (2019). Tissue-specific and geographical variation in endophytic fungi of Ageratina adenophora and fungal associations with the environment. Front. Microbiol., 10.","DOI":"10.3389\/fmicb.2019.02919"},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"57","DOI":"10.1007\/s00442-026-05894-6","article-title":"Leaf status drives shifts in both community composition and trophic mode of foliar endophytic fungi via variation in functional traits in Tibetan alpine meadow","volume":"208","author":"Kan","year":"2026","journal-title":"Oecologia"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"659","DOI":"10.1055\/s-2005-872943","article-title":"Beech leaf colonization by the endophyte Apiognomonia errabunda dramatically depends on light exposure and climatic conditions","volume":"7","author":"Bahnweg","year":"2005","journal-title":"Plant Biol."},{"key":"ref_44","doi-asserted-by":"crossref","unstructured":"Zheng, H., Liu, Q., Di, Y., Liu, T., Su, Y., He, Y., Chen, J., Peng, J., Lee, S.Y., and Hoa, I.T. (2025). Endophytic fungal diversity in Carpesium lipskyi from the Gaoligong Mountains, Yunnan, China. J. Fungi, 11.","DOI":"10.3390\/jof11100704"},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"1917","DOI":"10.1007\/s00203-021-02256-z","article-title":"Bioprospecting of endophytic microorganisms for bioactive compounds of therapeutic importance","volume":"203","author":"Raimi","year":"2021","journal-title":"Arch. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","unstructured":"He, C., Meng, D., Li, W., Li, X., and He, X. (2023). Dynamics of endophytic fungal communities associated with cultivated medicinal plants in farmland ecosystem. J. Fungi, 9.","DOI":"10.3390\/jof9121165"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"1190","DOI":"10.1016\/j.tim.2024.06.006","article-title":"Melanized root-associated fungi: Key players in plant-soil systems","volume":"32","author":"Netherway","year":"2024","journal-title":"Trends Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","unstructured":"Hil\u00e1rio, S., and Gon\u00e7alves, M.F.M. (2022). Endophytic Diaporthe as promising leads for the development of biopesticides and biofertilizers for a sustainable agriculture. Microorganisms, 10.","DOI":"10.3390\/microorganisms10122453"},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"105062","DOI":"10.1016\/j.arabjc.2023.105062","article-title":"Bioactive secondary metabolites produced by fungi of the genus Diaporthe (Phomopsis): Structures, biological activities, and biosynthesis","volume":"16","author":"Jiang","year":"2023","journal-title":"Arab. J. Chem."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"e70268","DOI":"10.1002\/mbo3.70268","article-title":"Bridging the cultivation gap in plant microbiomes: A comparative study of aerial root mucilage microbiome characterization by conventional isolation, prospector high-throughput cultivation, and molecular profiling","volume":"15","author":"Quattrone","year":"2026","journal-title":"MicrobiologyOpen"},{"key":"ref_51","first-page":"106","article-title":"Identification and AChE Inhibitory Activity of Culturable Endophytic Fungi Isolated from Polygala sibirica","volume":"44","author":"Shen","year":"2024","journal-title":"J. Southwest For. Univ."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Son, S.Y., Lee, S., Singh, D., Lee, N.R., Lee, D.Y., and Lee, C.H. (2018). Comprehensive secondary metabolite profiling toward delineating the solid and submerged-state fermentation of Aspergillus oryzae KCCM 12698. Front. Microbiol., 9.","DOI":"10.3389\/fmicb.2018.01076"},{"key":"ref_53","doi-asserted-by":"crossref","unstructured":"Shen, L., Roullier, C., Por\u00e9e, F.H., Gaslonde, T., Riffault-Valois, L., Grovel, O., Ruprich-Robert, G., and Chapeland-Leclerc, F. (2022). Complementary strategies to unlock Biosynthesis gene clusters encoding secondary metabolites in the filamentous fungus Podospora anserina. J. Fungi, 9.","DOI":"10.3390\/jof9010009"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/9\/669\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,7]],"date-time":"2026-09-07T12:54:50Z","timestamp":1788785690000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/12\/9\/669"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,6]]},"references-count":53,"journal-issue":{"issue":"9","published-online":{"date-parts":[[2026,9]]}},"alternative-id":["jof12090669"],"URL":"https:\/\/doi.org\/10.3390\/jof12090669","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,6]]}}}