{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T09:10:56Z","timestamp":1789031456275,"version":"build-2803163510"},"reference-count":30,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T00:00:00Z","timestamp":1788998400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Hubei Provincial Joint Fund for Innovation and Development of Traditional Chinese Medicine","award":["2026AFC0923"],"award-info":[{"award-number":["2026AFC0923"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32500333"],"award-info":[{"award-number":["32500333"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["32500333"],"award-info":[{"award-number":["32500333"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"name":"Project of Hubei Province Traditional Chinese Medicine Innovation Team","award":["ZY2025J002"],"award-info":[{"award-number":["ZY2025J002"]}]},{"name":"key project at the central government level","award":["2060302"],"award-info":[{"award-number":["2060302"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Fungi","JoF"],"abstract":"<jats:p>Fungal biosynthetic gene clusters (BGCs) offer a genomic entry point for natural-product discovery, but predicted regions alone neither establish pathway activity nor identify metabolites. Armillaria is a useful model because chemically rich, forest-associated species span pathogenic and saprotrophic lifestyles. We analysed nine assemblies in a role-aware design: seven independent taxa with high completeness assessed using Benchmarking Universal Single-Copy Orthologs (BUSCO), an A. mellea BUSCO duplication-sensitivity sample and an A. solidipes intraspecific strain control for A. ostoyae. The analysis resolved a terpene-rich repertoire containing a shared but edge-robust gene cluster family (GCF) component alongside unevenly distributed candidate families. Targeted neighbourhood analysis recovered protoilludene synthase (PRO1) orthologs in all assemblies (92.2\u2013100.0% amino-acid identity); the core neighbourhood was retained across the seven primary taxa, whereas flanking tailoring-associated genes varied. Notably, the A. gallica PRO1 ortholog lay 23,614 bp outside the nearest antiSMASH region. Thus, whole-region prediction can obscure pathway-centred conservation in basidiomycetes. The resulting framework prioritises reproducible candidates while reserving chemical identity, expression and ecological function for direct validation.<\/jats:p>","DOI":"10.3390\/jof12090677","type":"journal-article","created":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T08:07:41Z","timestamp":1789027661000},"page":"677","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Comparative Phylogenomics Reveals Conserved Protoilludene Synthase Neighbourhoods and Unevenly Distributed Biosynthetic Gene Cluster Families in Armillaria"],"prefix":"10.3390","volume":"12","author":[{"given":"Huijun","family":"Su","sequence":"first","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine,  Wuhan 430065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ming","family":"Luo","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine,  Wuhan 430065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhuan","family":"Miao","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine,  Wuhan 430065, China"},{"name":"Hubei Shizhen Laboratory, Wuhan 430065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaohua","family":"Li","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine,  Wuhan 430065, China"},{"name":"Hubei Shizhen Laboratory, Wuhan 430065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dahui","family":"Liu","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine,  Wuhan 430065, China"},{"name":"Hubei Shizhen Laboratory, Wuhan 430065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei","family":"Su","sequence":"additional","affiliation":[{"name":"School of Pharmacy, Hubei University of Chinese Medicine,  Wuhan 430065, China"},{"name":"Hubei Shizhen Laboratory, Wuhan 430065, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,10]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"167","DOI":"10.1038\/s41579-018-0121-1","article-title":"Fungal secondary metabolism: Regulation, function and drug discovery","volume":"17","author":"Keller","year":"2019","journal-title":"Nat. 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