{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,23]],"date-time":"2026-09-23T05:04:34Z","timestamp":1790139874490,"version":"4.0.1"},"reference-count":51,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,18]],"date-time":"2026-09-18T00:00:00Z","timestamp":1789689600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Agencia Nacional de Investigaci\u00f3n e Innovaci\u00f3n","award":["POS_EXT_2021_1_172115"],"award-info":[{"award-number":["POS_EXT_2021_1_172115"]}],"id":[{"id":"https:\/\/ror.org\/03egaj678","id-type":"ROR","asserted-by":"crossref"}]},{"award":["POS_EXT_2021_1_172115"],"award-info":[{"award-number":["POS_EXT_2021_1_172115"]}],"id":[{"id":"https:\/\/ror.org\/03egaj678","id-type":"ROR","asserted-by":"publisher"}]},{"name":"INIA","award":["RTA2017-00009-C04-04"],"award-info":[{"award-number":["RTA2017-00009-C04-04"]}]},{"award":["RTA2017-00009-C04-04"],"award-info":[{"award-number":["RTA2017-00009-C04-04"]}],"id":[{"id":"https:\/\/ror.org\/011q66e29","id-type":"ROR","asserted-by":"publisher"}]},{"name":"ERDF","award":["PID2020-114648RR-C33"],"award-info":[{"award-number":["PID2020-114648RR-C33"]}]},{"name":"ERDF","award":["MCIN\/AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033"]}]},{"award":["PID2020-114648RR-C33"],"award-info":[{"award-number":["PID2020-114648RR-C33"]}],"id":[{"id":"https:\/\/ror.org\/01rkhv978","id-type":"ROR","asserted-by":"publisher"}]},{"award":["MCIN\/AEI\/10.13039\/501100011033"],"award-info":[{"award-number":["MCIN\/AEI\/10.13039\/501100011033"]}],"id":[{"id":"https:\/\/ror.org\/01rkhv978","id-type":"ROR","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Fungi","JoF"],"abstract":"<jats:p>This study evaluated the in vitro and in planta efficacy of six chemical fungicides (boscalid, captan, copper oxychloride, difenoconazole, pyraclostrobin, and potassium hydrogen carbonate) and two biological control agents (Bacillus amyloliquefaciens subsp. plantarum D747 and Trichoderma atroviride strain SC1) against Diaporthe amygdali, the causal agent of twig canker and shoot blight disease of almond. In vitro assays demonstrated that D. amygdali is highly sensitive to captan, difenoconazole, and pyraclostrobin. Regarding biological products, dual antagonism assays showed high efficacy for T. atroviride. Subsequently, the pre- and post-infection activity of all products was assessed on one-year-old almond plants. Efficacy varied significantly depending on the timing of application. Trichoderma atroviride was effective at all application times, achieving mean percent disease control (MPDC) values of 100% when applied preventively. Pre-infection applications of difenoconazole and pyraclostrobin resulted in 100% MPDC. However, their efficacy progressively decreased when applied post-infection. Bacillus amyloliquefaciens and captan showed variable results, reaching a maximum of 73% and 87% MPDC, respectively, when applied before inoculation. In contrast, boscalid, copper oxychloride, and potassium hydrogen carbonate exhibited very low MPDC values, regardless of application timing. These findings provide valuable information for improving management strategies against D. amygdali in almond orchards.<\/jats:p>","DOI":"10.3390\/jof12090700","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:09:35Z","timestamp":1790075375000},"page":"700","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Management of Twig Canker and Shoot Blight of Almond Caused by Diaporthe amygdali: Efficacy of Chemical Fungicides and Biological Control Agents"],"prefix":"10.3390","volume":"12","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-4729-3652","authenticated-orcid":false,"given":"Carolina","family":"Francia","sequence":"first","affiliation":[{"name":"Instituto Nacional de Investigaci\u00f3n Agropecuaria, INIA La Estanzuela, Colonia 70000, Uruguay"},{"name":"Instituto Agroforestal Mediterr\u00e1neo, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3815-8578","authenticated-orcid":false,"given":"Josep","family":"Armengol","sequence":"additional","affiliation":[{"name":"Instituto Agroforestal Mediterr\u00e1neo, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Antonio","family":"Ram\u00f3n","sequence":"additional","affiliation":[{"name":"Instituto Agroforestal Mediterr\u00e1neo, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5773-3104","authenticated-orcid":false,"given":"M\u00f3nica","family":"Berbegal","sequence":"additional","affiliation":[{"name":"Instituto Agroforestal Mediterr\u00e1neo, Universitat Polit\u00e8cnica de Val\u00e8ncia, 46022 Valencia, Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,18]]},"reference":[{"key":"ref_1","unstructured":"Arquero, O., Belmonte, A., Casado, B., Cruz-Blanco, M., Espadafor, M., Fern\u00e1ndez, J.L., Gallego, J.C., Garc\u00eda, A., Lorite, I., and Lovera, M. (2013). Manual Del Almendro, Junta de Andalucia."},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Calder\u00f3n-Pav\u00f3n, A., Francisco Garc\u00eda-Tejero, I., Noguera-Artiaga, L., Lipan, L., Sendra, E., Hern\u00e1ndez, F., Francisco Herencia-Gal\u00e1n, J., Antonio Carbonell-Barrachina, \u00c1., and Hugo Dur\u00e1n Zuazo, V. (2025). Linking Almond Yield and Quality to the Production System and Irrigation Strategy Considering the Plantation Age in a Mediterranean Semiarid Environment. Agronomy, 15.","DOI":"10.3390\/agronomy15061448"},{"key":"ref_3","first-page":"36","article-title":"An\u00e1lisis de La Evoluci\u00f3n Territorial y Varietal Del Cultivo Del Almendro En Espa\u00f1a","volume":"404","author":"Egea","year":"2015","journal-title":"Vida Rural"},{"key":"ref_4","unstructured":"Malag\u00f3n, J. (2020). Cultivo Del Almendro, Direcci\u00f3n General de Desarrollo Rural."},{"key":"ref_5","first-page":"307","article-title":"Emerging Diseases of Almond Crop in Southern Spain","volume":"1","author":"Lovera","year":"2024","journal-title":"Acta Hortic."},{"key":"ref_6","first-page":"128","article-title":"El Futuro de La Sanidad Vegetal Del Almendro","volume":"49","year":"2016","journal-title":"Fruticultura"},{"key":"ref_7","first-page":"166","article-title":"Las Enfermedades Del Almendro En Andaluc\u00eda","volume":"49","author":"Lovera","year":"2016","journal-title":"Fruticultura"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1007\/s13225-010-0057-x","article-title":"Phylogeny, Morphology and Pathogenicity of Diaporthe and Phomopsis Species on Almond in Portugal","volume":"44","author":"Diogo","year":"2010","journal-title":"Fungal Divers."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"987","DOI":"10.1007\/s42161-023-01420-2","article-title":"Elucidating the Almond Constriction Canker Caused by Diaporthe amygdali in Sicily (South Italy)","volume":"105","author":"Gusella","year":"2023","journal-title":"J. Plant Pathol."},{"key":"ref_10","doi-asserted-by":"crossref","unstructured":"Le\u00f3n, M., Berbegal, M., Rodr\u00edguez-Reina, J.M., Elena, G., Abad-Campos, P., Ram\u00f3n-Albalat, A., Olmo, D., Vicent, A., Luque, J., and Miarnau, X. (2020). Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain. Agronomy, 10.","DOI":"10.3390\/agronomy10081062"},{"key":"ref_11","first-page":"532","article-title":"Phomopsis Blight on Almond in Hungary (Pathogen: Phomopsis amygdali, Teleomorph: Diaporthe amygdali)","volume":"53","author":"Varjas","year":"2017","journal-title":"N\u00f6v\u00e9nyv\u00e9delem"},{"key":"ref_12","first-page":"384","article-title":"Aktualni Problemi u Za\u0161titi Intenzivnih Nasada Bajama Od Bolesti i \u0160tetnika","volume":"17","year":"2017","journal-title":"Glas. Biljn. Za\u0161t."},{"key":"ref_13","unstructured":"Teviotdale, B.L., Michailides, T.J., and Pscheidt, J.W. (2002). Phomopsis Canker and Fruit Rot. Compendium of Nut Crop Diseases in Temperate Zones, APS Press."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1094\/PDIS.2002.86.7.721","article-title":"Effect of Fungicides, Application Timing, and Canker Removal on Incidence and Severity of Constriction Canker of Peach","volume":"86","author":"Lalancette","year":"2002","journal-title":"Plant Dis."},{"key":"ref_15","unstructured":"Gil, A.M., Arribas Carrasco, G., and Barrios Sanrom\u00e0, G. (2015). Gu\u00eda de Gesti\u00f3n Integrada de Plagas: Almendro, Ministerio de Agricultura, Alimentaci\u00f3n y Medio Ambiente."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"124746","DOI":"10.1016\/j.colsurfa.2020.124746","article-title":"Formulation Optimization of D-Limonene-Loaded Nanoemulsions as a Natural and Efficient Biopesticide","volume":"596","author":"Feng","year":"2020","journal-title":"Colloids Surf. A Physicochem. Eng. Asp."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"559","DOI":"10.1007\/s10658-022-02627-2","article-title":"Biocontrol Potential of Bacillus licheniformis W10 against Peach Shoot Blight Caused by Phomopsis amygdali","volume":"165","author":"Ji","year":"2023","journal-title":"Eur. J. Plant Pathol."},{"key":"ref_18","first-page":"69","article-title":"Chemical and Biological Control of Phomopsis amygdali the Causal Agent of Contriction Canker of Almond in Tunisia","volume":"3","author":"Rhouma","year":"2008","journal-title":"Tunis. J. Plant Prot."},{"key":"ref_19","first-page":"6","article-title":"Constriction Canker Management in Peach","volume":"3","author":"Schnabel","year":"2003","journal-title":"Southeast. Reg. Peach Newsl."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"194","DOI":"10.1111\/j.1439-0434.2008.01469.x","article-title":"Contribution of Pathogens to Peach Fruit Rot in Northern Greece and Their Sensitivity to Iprodione, Carbendazim, Thiophanate-Methyl and Tebuconazole Fungicides","volume":"157","author":"Thomidis","year":"2009","journal-title":"J. Phytopathol."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"449","DOI":"10.1111\/j.1365-2338.1997.tb00664.x","article-title":"Incidence of Phomopsis amygdali, Botryosphaeria berengeriana and Valsa cincta Diseases in Almond under Different Control Strategies","volume":"27","author":"Tuset","year":"1997","journal-title":"EPPO Bull."},{"key":"ref_22","first-page":"281","article-title":"Virulence and Control Efficacy in Field of Fungicides on Phomopsis amygdali of Peach","volume":"30","author":"ZhaoLin","year":"2013","journal-title":"J. Fruit Sci."},{"key":"ref_23","doi-asserted-by":"crossref","unstructured":"Oliver, R.P., and Beckerman, J.L. (2022). Fungicides in Practice, CABI.","DOI":"10.1079\/9781789246926.0000"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"707","DOI":"10.1094\/PHYTO-10-22-0370-KD","article-title":"Fungicide Resistance: Progress in Understanding Mechanism, Monitoring, and Management","volume":"113","author":"Yin","year":"2023","journal-title":"Phytopathology"},{"key":"ref_25","doi-asserted-by":"crossref","unstructured":"European Food Safety Authority (EFSA), Bellisai, G., Bernasconi, G., Binaglia, M., Carrasco Cabrera, L., Castellan, I., Castoldi, A.F., Santonja, G.G., Greco, L., and Istace, F. (2024). Updated Reasoned Opinion on the Toxicological Properties and Maximum Residue Levels (MRLs) for the Benzimidazole Substances Carbendazim and Thiophanate-Methyl. EFSA J., 22, e8569.","DOI":"10.2903\/j.efsa.2024.8569"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1007\/s13225-013-0245-6","article-title":"Diaporthe Species Occurring on Citrus in China","volume":"61","author":"Huang","year":"2013","journal-title":"Fungal Divers."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Francia, C., L\u00e1zaro, E., Novell\u00f3n, M., Ram\u00f3n-Albalat, A., Beluz\u00e1n, F., Vicent, A., Berbegal, M., and Armengol, J. (2025). Biology and Epidemiology of Diaporthe amygdali: Understanding How Environmental Factors Influence Fungal Growth, Sporulation, Infection and Lesion Development on Almond. Front. Plant Sci., 16.","DOI":"10.3389\/fpls.2025.1717223"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1890","DOI":"10.1094\/PDIS-09-21-1875-RE","article-title":"Susceptibility of Almond (Prunus dulcis) Cultivars to Twig Canker and Shoot Blight Caused by Diaporthe amygdali","volume":"106","author":"Miarnau","year":"2022","journal-title":"Plant Dis."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Lucchese, P.G., Dlalah, N., Buisine, A., Nigro, F., Pollastro, S., and Duval, H. (2026). Study on the Susceptibility of Some Almond (Prunus dulcis) Cultivars to the Pathogen Diaporthe amygdali. Plants, 15.","DOI":"10.3390\/plants15010165"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1038\/s41438-020-00444-4","article-title":"Pedigree Analysis of 220 Almond Genotypes Reveals Two World Mainstream Breeding Lines Based on Only Three Different Cultivars","volume":"8","author":"Romero","year":"2021","journal-title":"Hortic. Res."},{"key":"ref_31","doi-asserted-by":"crossref","unstructured":"Turco, S., Brugneti, F., Cardacino, A., and Mazzaglia, A. (2025). Harnessing Genomics of Diaporthe amygdali for Improved Control of Peach Twig Canker and Shoot Blight (TCSB). Plants, 14.","DOI":"10.3390\/plants14192960"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"785","DOI":"10.1111\/ppa.13525","article-title":"Thiophanate-Methyl and Carbendazim Resistance in Fusicoccum amygdali, the Causal Agent of Constriction Canker of Peach and Almond","volume":"71","author":"Fontaine","year":"2022","journal-title":"Plant Pathol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"705","DOI":"10.1094\/PDIS-06-18-1052-RE","article-title":"Investigation of Fungi Causing Twig Blight Diseases on Peach Trees in South Carolina","volume":"103","author":"Froelich","year":"2019","journal-title":"Plant Dis."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"199","DOI":"10.1146\/annurev-phyto-080614-120357","article-title":"Soil Health Paradigms and Implications for Disease Management","volume":"53","author":"Larkin","year":"2015","journal-title":"Annu. Rev. Phytopathol."},{"key":"ref_35","unstructured":"(2025, July 01). MAPA Consultas Fitosanitarias. Available online: https:\/\/servicio.mapa.gob.es\/regfiweb#."},{"key":"ref_36","unstructured":"De Li\u00f1\u00e1n, C. (2024). Vademecum de Productos Fitosanitarios y Nutricionales, TecnoAgr\u00edcola de Espa\u00f1a."},{"key":"ref_37","doi-asserted-by":"crossref","unstructured":"Rueden, C.T., Schindelin, J., Hiner, M.C., DeZonia, B.E., Walter, A.E., Arena, E.T., and Eliceiri, K.W. (2017). ImageJ2: ImageJ for the next Generation of Scientific Image Data. BMC Bioinform., 18.","DOI":"10.1186\/s12859-017-1934-z"},{"key":"ref_38","first-page":"425","article-title":"Characterization of Trichoderma Isolates from Southern Italy, and Their Potential Biocontrol Activity against Grapevine Trunk Disease Fungi","volume":"59","author":"Tomaselli","year":"2020","journal-title":"Phytopathol. Mediterr."},{"key":"ref_39","unstructured":"Posit Team (2025). RStudio: Integrated Development Environment for R. Posit Software, PBC. Available online: https:\/\/posit.co\/Posit."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"378","DOI":"10.32614\/RJ-2017-066","article-title":"GlmmTMB Balances Speed and Flexibility among Packages for Zero-Inflated Generalized Linear Mixed Modeling","volume":"9","author":"Brooks","year":"2017","journal-title":"R J."},{"key":"ref_41","unstructured":"Lenth, R.V., and Piaskowski, J. (2026, February 05). Emmeans: Estimated Marginal Means, Aka Least-Squares Means. Available online: https:\/\/cran.r-project.org\/package=emmeans."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"346","DOI":"10.1002\/bimj.200810425","article-title":"Simultaneous Inference in General Parametric Models","volume":"50","author":"Hothorn","year":"2008","journal-title":"Biom. J."},{"key":"ref_43","unstructured":"Kassambara, A. (2026, July 23). Rstatix: Pipe-Friendly Framework for Basic Statistical Tests. Available online: https:\/\/cran.r-project.org\/web\/packages\/rstatix\/index.html."},{"key":"ref_44","unstructured":"Graves, S., Piepho, H.-P., and Selzer, L. (2026, July 23). MultcompView: Visualizations of Paired Comparisons. Available online: https:\/\/cran.r-project.org\/package=multcompView."},{"key":"ref_45","doi-asserted-by":"crossref","first-page":"649","DOI":"10.1094\/PHYTO-02-25-0047-R","article-title":"Effect of Weather Variables on the Inoculum of Diaporthe amygdali Causal Agent of Twig Canker and Shoot Blight in Almond Orchards","volume":"115","author":"Vicent","year":"2025","journal-title":"Phytopathology"},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3368","DOI":"10.1094\/PDIS-11-20-2371-RE","article-title":"Evaluation of Pruning Wound Protection Products for the Management of Almond Canker Diseases in California","volume":"105","author":"Holland","year":"2021","journal-title":"Plant Dis."},{"key":"ref_47","first-page":"77","article-title":"Evaluation of Fungicides to Protect Pruning Wounds from Botryosphaeriaceae Species Infections on Almond Trees","volume":"56","author":"Olmo","year":"2017","journal-title":"Phytopathol. Mediterr."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"211","DOI":"10.1007\/s42161-023-01546-3","article-title":"Control of Monilinia Blossom and Twig Blight (Monilinia laxa) by Boron, Pyroligneous Acid and Boscalid","volume":"106","author":"Uysal","year":"2023","journal-title":"J. Plant Pathol."},{"key":"ref_49","unstructured":"Adaskaveg, J.E., Michailides, T., and Eskalen, A. (2022). Fungicides, Bactericides, Biocontrols, and Natural Products for Deciduous Tree Fruit and Nut, Citrus, Strawberry, and Vine Crops in California 2022, University of California Agriculture and Natural Resources."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1094\/PHYTO-02-23-0075-R","article-title":"Evaluation of Biological Control Agents for the Protection of Almond Pruning Wounds against Infection by Fungal Canker Pathogens","volume":"113","author":"Travadon","year":"2023","journal-title":"Phytopathology"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"105292","DOI":"10.1016\/j.biocontrol.2023.105292","article-title":"Field Evaluation of Biological Wound Treatments for the Management of Almond, Cherry, and Grapevine Fungal Canker Diseases","volume":"185","author":"Travadon","year":"2023","journal-title":"Biol. 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