{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T12:15:52Z","timestamp":1790079352159,"version":"4.0.1"},"reference-count":68,"publisher":"MDPI AG","issue":"9","license":[{"start":{"date-parts":[[2026,9,21]],"date-time":"2026-09-21T00:00:00Z","timestamp":1789948800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["U24A20745"],"award-info":[{"award-number":["U24A20745"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"award":["U24A20745"],"award-info":[{"award-number":["U24A20745"]}],"id":[{"id":"https:\/\/ror.org\/01h0zpd94","id-type":"ROR","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100020084","name":"Key Research and Development Plan of the Guangzhou Science and Technology Bureau","doi-asserted-by":"publisher","award":["2024B01J1191"],"award-info":[{"award-number":["2024B01J1191"]}],"id":[{"id":"10.13039\/501100020084","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["J. Fungi","JoF"],"abstract":"<jats:p>Sporothrix globosa is the predominant cause of sporotrichosis in China, highlighting the need for effective vaccine strategies. Cyclophilins from pathogenic microorganisms have emerged as potential vaccine antigens. We previously generated a high-yield, high-purity recombinant truncated S. globosa cyclophilin B protein (SgtrCypB) retaining its functional domain. Here, BALB\/c mice were immunized subcutaneously with SgtrCypB alone or formulated with Freund\u2019s adjuvant to evaluate immunogenicity. Only the adjuvant-formulated vaccine induced a high antibody titer (1:51,200). Then, the infection-challenge experiments compared the SgtrCypB formulated with Freund\u2019s adjuvant and PBS formulated with Freund\u2019s adjuvant groups. Following challenge, SgtrCypB plus adjuvant reduced cutaneous lesion size (0.6 vs. 1.0 cm at week 1) without significantly affecting ulcer incidence. In systemic S. globosa infection, vaccination increased survival to 90% versus 40% in controls and reduced organ fungal burdens. Partial cross-protection was observed against S. schenckii, with 60% versus 20% survival and lower fungal burdens in the kidneys and lungs. Protection against C. albicans was minimal. These findings identify SgtrCypB as a promising protective antigen against S. globosa and S. schenckii and support further evaluation with clinically applicable adjuvants.<\/jats:p>","DOI":"10.3390\/jof12090706","type":"journal-article","created":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T11:09:35Z","timestamp":1790075375000},"page":"706","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["SgtrCypB Formulated with Freund\u2019s Adjuvant Confers Protective Immunity Against S. globosa and Partial Cross-Protection Against S. schenckii Infections in Mice"],"prefix":"10.3390","volume":"12","author":[{"given":"Ling","family":"Hu","sequence":"first","affiliation":[{"name":"Department of Dermatology and Venereology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510600, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"methodology","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"},{"role":"writing-review-editing","vocabulary":"credit"}]},{"given":"Baicheng","family":"Deng","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"software","vocabulary":"credit"},{"role":"data-curation","vocabulary":"credit"}]},{"given":"Yuyan","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510600, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"formal-analysis","vocabulary":"credit"},{"role":"writing-original-draft","vocabulary":"credit"}]},{"given":"Meizhen","family":"Zhong","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510600, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"validation","vocabulary":"credit"},{"role":"investigation","vocabulary":"credit"}]},{"given":"Xiaoyu","family":"Zhi","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510600, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"visualization","vocabulary":"credit"}]},{"given":"Xuchu","family":"Hu","sequence":"additional","affiliation":[{"name":"Department of Parasitology, Zhongshan School of Medicine, Sun Yat-Sen University, Guangzhou 510000, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"writing-review-editing","vocabulary":"credit"},{"role":"supervision","vocabulary":"credit"},{"role":"conceptualization","vocabulary":"credit"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6320-8031","authenticated-orcid":false,"given":"Huaiqiu","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venereology, The Third Affiliated Hospital of Sun Yat-Sen University, Guangzhou 510600, China"}],"role":[{"vocabulary":"crossref","role":"author"},{"role":"conceptualization","vocabulary":"credit"},{"role":"funding-acquisition","vocabulary":"credit"},{"role":"project-administration","vocabulary":"credit"}]}],"member":"1968","published-online":{"date-parts":[[2026,9,21]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1007\/s11046-020-00425-0","article-title":"The threat of emerging and re-emerging pathogenic Sporothrix species","volume":"185","author":"Rodrigues","year":"2020","journal-title":"Mycopathologia"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e13806","DOI":"10.1111\/myc.13806","article-title":"Sporothrix and Sporotrichosis: A South African Perspective on a Growing Global Health Threat","volume":"67","author":"Fuchs","year":"2024","journal-title":"Mycoses"},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"407","DOI":"10.1007\/s11046-022-00642-9","article-title":"A Spotlight on Sporothrix and Sporotrichosis","volume":"187","author":"Rodrigues","year":"2022","journal-title":"Mycopathologia"},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"4863","DOI":"10.2147\/IJGM.S531445","article-title":"Updates on Current Situation of Cutaneous Sporotrichosis: A Narrative Review","volume":"18","author":"Shi","year":"2025","journal-title":"Int. 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