{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,29]],"date-time":"2025-11-29T07:53:36Z","timestamp":1764402816526,"version":"build-2065373602"},"reference-count":55,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2018,3,15]],"date-time":"2018-03-15T00:00:00Z","timestamp":1521072000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The fungal pathogens Cryptococcus neoformans and Cryptococcus gattii can cause life-threatening infections in immune compromised and immune competent hosts. These pathogens enter the host via inhalation, and respiratory tract innate immune cells such as dendritic cells (DCs) are one of the first host cells they encounter. The interactions between Cryptococcus and innate immune cells play a critical role in the progression of disease in the host. This review will focus specifically on the interactions between Cryptococcus and dendritic cells (DCs), including recognition\/processing by DCs, effects of immune mediators on DC recruitment and activity, and the potential for DC vaccination against cryptococcosis.<\/jats:p>","DOI":"10.3390\/jof4010036","type":"journal-article","created":{"date-parts":[[2018,3,15]],"date-time":"2018-03-15T05:11:28Z","timestamp":1521090688000},"page":"36","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":18,"title":["Interactions of Cryptococcus with Dendritic Cells"],"prefix":"10.3390","volume":"4","author":[{"given":"Karen","family":"Wozniak","sequence":"first","affiliation":[{"name":"Department of Microbiology and Molecular Genetics, Oklahoma State University, Stillwater, OK 74078, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,3,15]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"158","DOI":"10.4049\/jimmunol.165.1.158","article-title":"Dendritic cells in the induction of protective and nonprotective anticryptococcal cell-mediated immune responses","volume":"165","author":"Bauman","year":"2000","journal-title":"J. Immunol."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1675","DOI":"10.4049\/jimmunol.129.4.1675","article-title":"The capsule of Cryptococcus neoformans passively inhibits phagocytosis of the yeast by macrophages","volume":"129","author":"Kozel","year":"1982","journal-title":"J. Immunol."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"S436","DOI":"10.1093\/cid\/10.Supplement_2.S436","article-title":"Role of the capsule in phagocytosis of Cryptococcus neoformans","volume":"10","author":"Kozel","year":"1988","journal-title":"Rev. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"1107","DOI":"10.2217\/fmb.13.84","article-title":"Elucidating the immunological function of the Cryptococcus neoformans capsule","volume":"8","author":"Vecchiarelli","year":"2013","journal-title":"Future Microbiol."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"592","DOI":"10.1128\/IAI.73.1.592-598.2005","article-title":"Opsonic requirements for dendritic cell-mediated responses to Cryptococcus neoformans","volume":"73","author":"Kelly","year":"2005","journal-title":"Infect. Immun."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"5373","DOI":"10.1128\/IAI.72.9.5373-5382.2004","article-title":"Involvement of CD14, toll-like receptors 2 and 4, and MyD88 in the host response to the fungal pathogen Cryptococcus neoformans in vivo","volume":"72","author":"Yauch","year":"2004","journal-title":"Infect. Immun."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"4620","DOI":"10.4049\/jimmunol.166.7.4620","article-title":"Toll-like receptor 4 mediates intracellular signaling without TNF-\u03b1 release in response to Cryptococcus neoformans polysaccharide capsule","volume":"166","author":"Shoham","year":"2001","journal-title":"J. Immunol."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1111\/j.1574-695X.2006.00078.x","article-title":"Limited contribution of toll-like receptor 2 and 4 to the host response to a fungal infectious pathogen, Cryptococcus neoformans","volume":"47","author":"Nakamura","year":"2006","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"4067","DOI":"10.4049\/jimmunol.180.6.4067","article-title":"Deoxynucleic acids from Cryptococcus neoformans activate myeloid dendritic cells via a TLR9-dependent pathway","volume":"180","author":"Nakamura","year":"2008","journal-title":"J. Immunol."},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"778","DOI":"10.1128\/IAI.05570-11","article-title":"Toll-like receptor 9-dependent activation of bone marrow-derived dendritic cells by URA5 DNA from Cryptococcus neoformans","volume":"80","author":"Tanaka","year":"2012","journal-title":"Infect. Immun."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"363","DOI":"10.1111\/j.1574-695X.2011.00859.x","article-title":"Cryptococcus neoformans suppresses the activation of bone marrow-derived dendritic cells stimulated with its own DNA, but not with DNA from other fungi","volume":"63","author":"Yamamoto","year":"2011","journal-title":"FEMS Immunol. Med. Microbiol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"754","DOI":"10.2353\/ajpath.2010.091104","article-title":"TLR9 signaling is required for generation of the adaptive immune protection in Cryptococcus neoformans-infected lungs","volume":"177","author":"Zhang","year":"2010","journal-title":"Am. J. Pathol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Wang, J.P., Lee, C.K., Akalin, A., Finberg, R.W., and Levitz, S.M. (2011). Contributions of the MyD88-dependent receptors IL-18r, IL-1R, and TLR9 to host defenses following pulmonary challenge with Cryptococcus neoformans. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0026232"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"817","DOI":"10.1038\/ni.2369","article-title":"C-type lectin receptors orchestrate antifungal immunity","volume":"13","author":"Hardison","year":"2012","journal-title":"Nat. Immunol."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1115","DOI":"10.1111\/j.1348-0421.2007.tb04007.x","article-title":"Dectin-1 is not required for the host defense to Cryptococcus neoformans","volume":"51","author":"Nakamura","year":"2007","journal-title":"Microbiol. Immunol."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"671","DOI":"10.1128\/IAI.02835-14","article-title":"Dectin-2 deficiency promotes Th2 response and mucin production in the lungs after pulmonary infection with Cryptococcus neoformans","volume":"83","author":"Nakamura","year":"2015","journal-title":"Infect. Immun."},{"key":"ref_17","doi-asserted-by":"crossref","unstructured":"Campuzano, A., Castro-Lopez, N., Wozniak, K.L., Leopold Wager, C.M., and Wormley, F.L. (2017). Dectin-3 is not required for protection against Cryptococcus neoformans infection. PLoS ONE, 12.","DOI":"10.1371\/journal.pone.0169347"},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"820","DOI":"10.1128\/IAI.73.2.820-827.2005","article-title":"Mannoproteins from Cryptococcus neoformans promote dendritic cell maturation and activation","volume":"73","author":"Pietrella","year":"2005","journal-title":"Infect. Immun."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"3053","DOI":"10.4049\/jimmunol.176.5.3053","article-title":"Cryptococcus neoformans glycoantigens are captured by multiple lectin receptors and presented by dendritic cells","volume":"176","author":"Mansour","year":"2006","journal-title":"J. Immunol."},{"key":"ref_20","doi-asserted-by":"crossref","unstructured":"Dan, J.M., Wang, J.P., Lee, C.K., and Levitz, S.M. (2008). Cooperative stimulation of dendritic cells by Cryptococcus neoformans mannoproteins and CpG oligodeoxynucleotides. PLoS ONE, 3.","DOI":"10.1371\/journal.pone.0002046"},{"key":"ref_21","doi-asserted-by":"crossref","unstructured":"Wozniak, K.L., Ravi, S., Macias, S., Young, M.L., Olszewski, M.A., Steele, C., and Wormley, F.L. (2009). Insights into the mechanisms of protective immunity against Cryptococcus neoformans infection using a mouse model of pulmonary cryptococcosis. PLoS ONE, 4.","DOI":"10.1371\/journal.pone.0006854"},{"key":"ref_22","doi-asserted-by":"crossref","unstructured":"Wozniak, K.L., Hardison, S.E., Kolls, J.K., and Wormley, F.L. (2011). Role of IL-17A on resolution of pulmonary C. neoformans infection. PLoS ONE, 6.","DOI":"10.1371\/journal.pone.0017204"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"937","DOI":"10.1128\/IAI.01477-13","article-title":"Interleukin-17A enhances host defense against cryptococcal lung infection through effects mediated by leukocyte recruitment, activation, and gamma interferon production","volume":"82","author":"Murdock","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Grahnert, A., Richter, T., Piehler, D., Eschke, M., Schulze, B., Muller, U., Protschka, M., Kohler, G., Sabat, R., and Brombacher, F. (2014). IL-4 receptor-\u03b1-dependent control of Cryptococcus neoformans in the early phase of pulmonary infection. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0087341"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"4107","DOI":"10.4049\/jimmunol.1400650","article-title":"Early or late IL-10 blockade enhances th1 and Th17 effector responses and promotes fungal clearance in mice with cryptococcal lung infection","volume":"193","author":"Murdock","year":"2014","journal-title":"J. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1810","DOI":"10.4049\/jimmunol.1501512","article-title":"Local GM-CSF-dependent differentiation and activation of pulmonary dendritic cells and macrophages protect against progressive cryptococcal lung infection in mice","volume":"196","author":"Chen","year":"2016","journal-title":"J. Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"3880","DOI":"10.1128\/IAI.01773-14","article-title":"Cryptococcus gattii infection dampens Th1 and Th17 responses by attenuating dendritic cell function and pulmonary chemokine expression in the immunocompetent hosts","volume":"82","author":"Angkasekwinai","year":"2014","journal-title":"Infect. Immun."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"610","DOI":"10.4049\/jimmunol.181.1.610","article-title":"CCR2 mediates conventional dendritic cell recruitment and the formation of bronchovascular mononuclear cell infiltrates in the lungs of mice infected with Cryptococcus neoformans","volume":"181","author":"Osterholzer","year":"2008","journal-title":"J. Immunol."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"8044","DOI":"10.4049\/jimmunol.0902823","article-title":"Accumulation of CD11b+ lung dendritic cells in response to fungal infection results from the CCR2-mediated recruitment and differentiation of Ly-6Chigh monocytes","volume":"183","author":"Osterholzer","year":"2009","journal-title":"J. Immunol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"238","DOI":"10.4049\/jimmunol.1203435","article-title":"Scavenger receptor a modulates the immune response to pulmonary Cryptococcus neoformans infection","volume":"191","author":"Qiu","year":"2013","journal-title":"J. Immunol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"1231","DOI":"10.3389\/fimmu.2017.01231","article-title":"Exploitation of scavenger receptor, macrophage receptor with collagenous structure, by Cryptococcus neoformans promotes alternative activation of pulmonary lymph node CD11b+ conventional dendritic cells and non-protective Th2 bias","volume":"8","author":"Xu","year":"2017","journal-title":"Front. Immunol."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3548","DOI":"10.4049\/jimmunol.1700057","article-title":"Scavenger receptor marco orchestrates early defenses and contributes to fungal containment during cryptococcal infection","volume":"198","author":"Xu","year":"2017","journal-title":"J. Immunol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"370","DOI":"10.1189\/jlb.1002476","article-title":"The polysaccharide capsule of Cryptococcus neoformans interferes with human dendritic cell maturation and activation","volume":"74","author":"Vecchiarelli","year":"2003","journal-title":"J. Leukoc. Biol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"1581","DOI":"10.1128\/IAI.01184-07","article-title":"The presence of capsule in Cryptococcus neoformans influences the gene expression profile in dendritic cells during interaction with the fungus","volume":"76","author":"Lupo","year":"2008","journal-title":"Infect. Immun."},{"key":"ref_35","doi-asserted-by":"crossref","unstructured":"Wiesner, D.L., Specht, C.A., Lee, C.K., Smith, K.D., Mukaremera, L., Lee, S.T., Lee, C.G., Elias, J.A., Nielsen, J.N., and Boulware, D.R. (2015). Chitin recognition via chitotriosidase promotes pathologic type-2 helper T cell responses to cryptococcal infection. PLoS Pathog., 11.","DOI":"10.1371\/journal.ppat.1004701"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"3817","DOI":"10.1128\/IAI.00317-06","article-title":"In vivo role of dendritic cells in a murine model of pulmonary cryptococcosis","volume":"74","author":"Wozniak","year":"2006","journal-title":"Infect. Immun."},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"4764","DOI":"10.1128\/IAI.00660-08","article-title":"Cryptococcus neoformans enters the endolysosomal pathway of dendritic cells and is killed by lysosomal components","volume":"76","author":"Wozniak","year":"2008","journal-title":"Infect. Immun."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"15945","DOI":"10.1073\/pnas.0607528103","article-title":"Recruitment of CD63 to Cryptococcus neoformans phagosomes requires acidification","volume":"103","author":"Love","year":"2006","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"739","DOI":"10.1038\/srep00739","article-title":"Mechanisms of dendritic cell lysosomal killing of Cryptococcus","volume":"2","author":"Hole","year":"2012","journal-title":"Sci. Rep."},{"key":"ref_40","unstructured":"Wozniak, K.L. (2018). Oklahoma State University, Stillwater, OK, USA, Unpublished work."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"249","DOI":"10.4049\/jimmunol.1202707","article-title":"Cryptococcus gattii is killed by dendritic cells, but evades adaptive immunity by failing to induce dendritic cell maturation","volume":"191","author":"Huston","year":"2013","journal-title":"J. Immunol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"1259","DOI":"10.4049\/jimmunol.1501089","article-title":"Cryptococcus gattii capsule blocks surface recognition required for dendritic cell maturation independent of internalization and antigen processing","volume":"196","author":"Huston","year":"2016","journal-title":"J. Immunol."},{"key":"ref_43","first-page":"101","article-title":"Evasion of innate immune responses by the highly virulent Cryptococcus gattii by altering capsule glucuronoxylomannan structure","volume":"5","author":"Urai","year":"2015","journal-title":"Front. Cell. Infect. Microbiol."},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"2493","DOI":"10.1128\/IAI.00103-16","article-title":"Antifungal activity of plasmacytoid dendritic cells against Cryptococcus neoformans in vitro requires expression of dectin-3 (CLEC4D) and reactive oxygen species","volume":"84","author":"Hole","year":"2016","journal-title":"Infect. Immun."},{"key":"ref_45","unstructured":"Wozniak, K.L., and Levitz, S.M. (2011). T-Cell and DC Responses to Cryptococcus, ASM Press."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"423","DOI":"10.1080\/08830180213274","article-title":"Cryptococcal infection and Th1-Th2 cytokine balance","volume":"21","author":"Koguchi","year":"2002","journal-title":"Int. Rev. Immunol."},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.fgb.2014.10.013","article-title":"Molecules at the interface of Cryptococcus and the host that determine disease susceptibility","volume":"78","author":"Wozniak","year":"2015","journal-title":"Fungal Genet. Biol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"1453","DOI":"10.1128\/IAI.00274-06","article-title":"Protection against cryptococcosis by using a murine gamma interferon-producing Cryptococcus neoformans strain","volume":"75","author":"Wormley","year":"2007","journal-title":"Infect. Immun."},{"key":"ref_49","doi-asserted-by":"crossref","unstructured":"Specht, C.A., Lee, C.K., Huang, H., Tipper, D.J., Shen, Z.T., Lodge, J.K., Leszyk, J., Ostroff, G.R., and Levitz, S.M. (2015). Protection against experimental cryptococcosis following vaccination with glucan particles containing Cryptococcus alkaline extracts. mBio, 6.","DOI":"10.1128\/mBio.01905-15"},{"key":"ref_50","doi-asserted-by":"crossref","unstructured":"Specht, C.A., Lee, C.K., Huang, H., Hester, M.M., Liu, J., Luckie, B.A., Torres Santana, M.A., Mirza, Z., Khoshkenar, P., and Abraham, A. (2017). Vaccination with recombinant Cryptococcus proteins in glucan particles protects mice against cryptococcosis in a manner dependent upon mouse strain and cryptococcal species. mBio, 8.","DOI":"10.1128\/mBio.01872-17"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"312","DOI":"10.4049\/jimmunol.1200603","article-title":"Relative contributions of dectin-1 and complement to immune responses to particulate \u03b2-glucans","volume":"189","author":"Huang","year":"2012","journal-title":"J. Immunol."},{"key":"ref_52","doi-asserted-by":"crossref","unstructured":"Huang, H., Ostroff, G.R., Lee, C.K., Specht, C.A., and Levitz, S.M. (2010). Robust stimulation of humoral and cellular immune responses following vaccination with antigen-loaded \u03b2-glucan particles. mBio, 1.","DOI":"10.1128\/mBio.00164-10"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"1585","DOI":"10.1128\/CVI.00463-13","article-title":"Characterization and optimization of the glucan particle-based vaccine platform","volume":"20","author":"Huang","year":"2013","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1577","DOI":"10.1128\/IAI.02827-14","article-title":"Dendritic cell-based immunization ameliorates pulmonary infection with highly virulent Cryptococcus gattii","volume":"83","author":"Ueno","year":"2015","journal-title":"Infect. Immun."},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"327","DOI":"10.1007\/978-1-4939-7104-6_22","article-title":"Immunization with antigen-pulsed dendritic cells against highly virulent Cryptococcus gattii infection: Analysis of cytokine-producing T cells","volume":"1625","author":"Ueno","year":"2017","journal-title":"Methods Mol. Biol."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/4\/1\/36\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T14:57:08Z","timestamp":1760194628000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/4\/1\/36"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,3,15]]},"references-count":55,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2018,3]]}},"alternative-id":["jof4010036"],"URL":"https:\/\/doi.org\/10.3390\/jof4010036","relation":{},"ISSN":["2309-608X"],"issn-type":[{"type":"electronic","value":"2309-608X"}],"subject":[],"published":{"date-parts":[[2018,3,15]]}}}