{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,12]],"date-time":"2025-10-12T04:00:34Z","timestamp":1760241634126,"version":"build-2065373602"},"reference-count":37,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2018,6,12]],"date-time":"2018-06-12T00:00:00Z","timestamp":1528761600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Certain endophytic fungi belonging to the Hypoxylon genus have recently been found to produce volatile organic compounds (VOCs) that have potential relevance as hydrocarbon fuels. Here, a recently discovered Hypoxylon sp. (BS15) was demonstrated to also produce VOCs, but with diminished VOC production after an extended period of in vitro growth. Restoring VOC production was partially achieved by growing BS15 in growth media containing finely ground woody tissue from the original host plant (Taxodium distichum). In an effort to isolate VOC production modulators, extracts from this woody tissue were made by sequentially extracting with dichloromethane, methanol, and water. Both the dichloromethane and water extracts were found to modulate VOC production, while the methanol extract had no effect. Surprisingly, the woody tissue remaining after exhaustive extraction was also shown to act as a VOC production modulator when included in the growth media, with changes observed in the production of four compounds. This woody tissue also induced production of two compounds not observed in the original BS15 extract. Filter paper had the same modulating effect as exhaustively extracted woody tissue, suggesting the modulation was perhaps due to cellulose degradation products. Overall, this study demonstrated that VOC production in BS15 can be influenced by multiple compounds in the woody tissue rather than a single modulator.<\/jats:p>","DOI":"10.3390\/jof4020069","type":"journal-article","created":{"date-parts":[[2018,6,12]],"date-time":"2018-06-12T10:58:32Z","timestamp":1528801112000},"page":"69","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Restoring Waning Production of Volatile Organic Compounds in the Endophytic Fungus Hypoxylon sp. (BS15)"],"prefix":"10.3390","volume":"4","author":[{"given":"Yuemin","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Chemistry, University of Central Florida, 4111 Libra Drive, Orlando, FL 32816, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8363-2019","authenticated-orcid":false,"given":"James K.","family":"Harper","sequence":"additional","affiliation":[{"name":"Department of Chemistry, University of Central Florida, 4111 Libra Drive, Orlando, FL 32816, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2018,6,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"274","DOI":"10.2307\/3545919","article-title":"Endophyte: The evolution of a term, and clarification of its use and definition","volume":"73","author":"Wilson","year":"1995","journal-title":"Oikos"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"491","DOI":"10.1128\/MMBR.67.4.491-502.2003","article-title":"Bioprospecting for microbial endophytes and their natural products","volume":"67","author":"Strobel","year":"2003","journal-title":"Microbiol. Mol. Biol. Rev."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"40","DOI":"10.2225\/vol3-issue1-fulltext-4","article-title":"Endophytic microorganisms: A review on insect control and recent advances on tropical plants","volume":"3","author":"Azevedo","year":"2000","journal-title":"Electron. J. Biotechnol."},{"key":"ref_4","first-page":"1346","article-title":"Mechanisms of fungal endophytes in plant protection against pathogens","volume":"4","author":"Gao","year":"2010","journal-title":"Afr. J. Microbiol. Res."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"463","DOI":"10.1016\/S0031-9422(00)00408-8","article-title":"Ambuic acid, a highly functionalized cyclohexenone with antifungal activity from Pestalotiopsis spp. and Monochaetia sp.","volume":"56","author":"Li","year":"2001","journal-title":"Phytochemistry"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"183","DOI":"10.1016\/S0378-1097(03)00426-9","article-title":"Kakadumycins, novel antibiotics from Streptomyces sp. NRRL 30566, an endophyte of Grevillea pteridifolia","volume":"224","author":"Castillo","year":"2003","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"3319","DOI":"10.1099\/mic.0.2008\/022186-0","article-title":"The production of myco-diesel hydrocarbons and their derivatives by the endophytic fungus Gliocladium roseum (NRRL 50072)","volume":"154","author":"Strobel","year":"2008","journal-title":"Microbiology"},{"key":"ref_8","first-page":"14","article-title":"Optimisation of engine operating parameters for eucalyptus oil mixed diesel fueled diesel engine using Taguchi method","volume":"6","author":"Tamilvendhan","year":"2011","journal-title":"ARPN J. Eng. Appl. Sci."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"235485","DOI":"10.1155\/2012\/235485","article-title":"Eucalyptus biodiesel as an alternative to diesel fuel: Preparation and tests on diesel engine","volume":"2012","author":"Tarabet","year":"2012","journal-title":"J. Biomed. Biotechnol."},{"key":"ref_10","unstructured":"Kazuo Sugito, K.S.T. (1981). Fuel Composition. (No. 4,297,109), U.S. Patent."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"903","DOI":"10.1007\/s00248-010-9759-6","article-title":"Hypoxylon sp., an endophyte of Persea indica, producing 1,8-cineole and other bioactive volatiles with fuel potential","volume":"60","author":"Tomsheck","year":"2010","journal-title":"Microb. Ecol."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"259","DOI":"10.1039\/c3np70129h","article-title":"Methods of discovery and techniques to study endophytic fungi producing fuel-related hydrocarbons","volume":"31","author":"Strobel","year":"2014","journal-title":"Nat. Prod. Rep."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"518","DOI":"10.1111\/ina.12350","article-title":"Volatile organic compounds emitted by filamentous fungi isolated from flooded homes after Hurricane Sandy show toxicity in a Drosophila bioassay","volume":"27","author":"Zhao","year":"2017","journal-title":"Indoor Air"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"243","DOI":"10.1016\/j.jscs.2012.02.007","article-title":"Separation and identification of hydrocarbons and other volatile compounds from cultures of Aspergillus niger by GC\u2013MS using two different capillary columns and solvents","volume":"19","author":"Siddiquee","year":"2015","journal-title":"J. Saudi Chem. Soc."},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Fiers, M., Lognay, G., Fauconnier, M.-L., and Jijakli, M.H. (2013). Volatile compound-mediated interactions between barley and pathogenic fungi in the soil. PLoS ONE, 8.","DOI":"10.1371\/journal.pone.0066805"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"891","DOI":"10.1002\/cbdv.201500284","article-title":"Study of the vapor phase over Fusarium fungi cultured on various substrates","volume":"13","author":"Gustyleva","year":"2016","journal-title":"Chem. Biodivers."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"973","DOI":"10.1007\/s10529-015-1773-9","article-title":"Bioprospecting\u2014Fuels from fungi","volume":"37","author":"Strobel","year":"2015","journal-title":"Biotechnol. Lett."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"165","DOI":"10.1007\/s11101-010-9163-3","article-title":"Muscodor species\u2014Endophytes with biological promise","volume":"10","author":"Strobel","year":"2011","journal-title":"Phytochem. Rev."},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"270","DOI":"10.1099\/mic.0.032540-0","article-title":"Volatile antimicrobials from Muscodor crispans, a novel endophytic fungus","volume":"156","author":"Mitchell","year":"2010","journal-title":"Microbiology"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"196","DOI":"10.1080\/21501203.2013.877990","article-title":"Muscodor kashayum sp. nov.\u2014A new volatile anti-microbial producing endophytic fungus","volume":"4","author":"Meshram","year":"2013","journal-title":"Mycology"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"93","DOI":"10.5248\/128.93","article-title":"Muscodor strobelii, a new endophytic species from South India","volume":"128","author":"Meshram","year":"2014","journal-title":"Mycotaxon"},{"key":"ref_22","first-page":"55","article-title":"Muscodor darjeelingensis, a new endophytic fungus of Cinnamomum camphora collected from northeastern Himalayas","volume":"66","author":"Saxena","year":"2014","journal-title":"Sydowia"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"47","DOI":"10.1007\/s13213-014-0834-y","article-title":"Muscodor tigerii sp. nov.-volatile antibiotic producing endophytic fungus from the Northeastern Himalayas","volume":"65","author":"Saxena","year":"2015","journal-title":"Ann. Microbiol."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1341","DOI":"10.1007\/s13213-012-0593-6","article-title":"Molecular and morphological evidence support four new species in the genus Muscodor from northern Thailand","volume":"63","author":"Suwannarach","year":"2013","journal-title":"Ann. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"319","DOI":"10.1007\/S10267-011-0165-9","article-title":"Muscodor sutura, a novel endophytic fungus with volatile antibiotic activities","volume":"53","author":"Kudalkar","year":"2012","journal-title":"Mycoscience"},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"797","DOI":"10.1016\/j.funbio.2010.07.006","article-title":"Muscodor fengyangensis sp. nov. from southeast China: Morphology, physiology and production of volatile compounds","volume":"114","author":"Zhang","year":"2010","journal-title":"Fungal Biol."},{"key":"ref_27","first-page":"229","article-title":"An endophytic Myrothecium inundatum producing volatile organic compounds","volume":"1","author":"Banerjee","year":"2010","journal-title":"Mycosphere"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"275","DOI":"10.1007\/s13213-013-0661-6","article-title":"Bionectria ochroleuca NOTL33\u2014An endophytic fungus from Nothapodytes foetida producing antimicrobial and free radical scavenging metabolites","volume":"64","author":"Samaga","year":"2014","journal-title":"Ann. Microbiol."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"Naznin, H.A., Kiyohara, D., Kimura, M., Miyazawa, M., Shimizu, M., and Hyakumachi, M. (2014). Systemic resistance induced by volatile organic compounds emitted by plant growth-promoting fungi in Arabidopsis thaliana. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0086882"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"729","DOI":"10.1007\/s00248-011-9818-7","article-title":"An endophytic Phomopsis sp. possessing bioactivity and fuel potential with its volatile organic compounds","volume":"61","author":"Singh","year":"2011","journal-title":"Microb. Ecol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"148","DOI":"10.1016\/j.micres.2006.03.013","article-title":"Volatile organic compounds released by the entomopathogenic fungus Beauveria bassiana","volume":"163","author":"Crespo","year":"2008","journal-title":"Microbiol. Res."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"3814","DOI":"10.1099\/mic.0.041327-0","article-title":"Volatile organic compound production by organisms in the genus Ascocoryne and a re-evaluation of myco-diesel production by NRRL 50072","volume":"156","author":"Griffin","year":"2010","journal-title":"Microbiology"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1111\/j.1574-6968.2011.02297.x","article-title":"An endophytic\/pathogenic Phoma sp. from creosote bush producing biologically active volatile compounds having fuel potential","volume":"320","author":"Strobel","year":"2011","journal-title":"FEMS Microbiol. Lett."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"465","DOI":"10.1099\/mic.0.054643-0","article-title":"Modulation of volatile organic compound formation in the Mycodiesel-producing endophyte Hypoxylon sp CI-4","volume":"158","author":"Strobel","year":"2012","journal-title":"Microbiology"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"1772","DOI":"10.1099\/mic.0.079756-0","article-title":"Functionalized para-substituted benzenes as 1,8-cineole production modulators in an endophytic Nodulisporium sp.","volume":"160","author":"Nigg","year":"2014","journal-title":"Microbiology"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"2943","DOI":"10.1099\/00221287-147-11-2943","article-title":"Volatile antimicrobials from Muscodor albus, a novel endophytic fungus","volume":"147","author":"Strobel","year":"2001","journal-title":"Microbiology"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"7540","DOI":"10.1074\/jbc.M211748200","article-title":"Carbohydrate-induced differential gene expression patterns in the hyperthermophilic bacterium Thremotoga maritima","volume":"278","author":"Chhabra","year":"2003","journal-title":"J. Biol. Chem."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/4\/2\/69\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T15:08:16Z","timestamp":1760195296000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/4\/2\/69"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,6,12]]},"references-count":37,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2018,6]]}},"alternative-id":["jof4020069"],"URL":"https:\/\/doi.org\/10.3390\/jof4020069","relation":{},"ISSN":["2309-608X"],"issn-type":[{"type":"electronic","value":"2309-608X"}],"subject":[],"published":{"date-parts":[[2018,6,12]]}}}