{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T20:37:15Z","timestamp":1780432635353,"version":"3.54.1"},"reference-count":36,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2019,4,12]],"date-time":"2019-04-12T00:00:00Z","timestamp":1555027200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"New Zealand Ministry of Health Oral Health Fund","award":["RF8.15"],"award-info":[{"award-number":["RF8.15"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Oral candidiasis is prevalent among older people due to predisposing factors such as impaired immune defenses, medications and denture use. An increasing number of older people live in rest home facilities and it is unclear how this institutionalized living affects the quantity and type of fungi colonizing these people\u2019s oral cavities. Smears and swabs of the palate and tongue and saliva samples were taken from participants residing in rest homes (RH; n = 20) and older people living in their own homes (OH; n = 20). Yeast in samples were quantified and identified by culturing on CHROMagar Candida and sequencing the ITS2 region of rDNA. A higher proportion of RH residents had Candida hyphae present in smears compared to OH participants (35% vs. 30%) although this difference was not statistically significant (p = 0.74). RH residents had, on average, 23 times as many yeast per mL saliva as OH participants (p = 0.01). Seven yeast species were identified in OH samples and only five in RH samples, with Candida albicans and Candida glabrata being the most common species isolated from both participant groups. The results indicate that older people living in aged-care facilities were more likely to have candidiasis and have a higher yeast carriage rate than similarly aged people living at home. This may be due to morbidities which led to the need for residential care and\/or related to the rest home environment.<\/jats:p>","DOI":"10.3390\/jof5020030","type":"journal-article","created":{"date-parts":[[2019,4,12]],"date-time":"2019-04-12T12:55:04Z","timestamp":1555073704000},"page":"30","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Yeast Species in the Oral Cavities of Older People: A Comparison between People Living in Their Own Homes and Those in Rest Homes"],"prefix":"10.3390","volume":"5","author":[{"given":"Nurulhuda Mohd","family":"Thiyahuddin","sequence":"first","affiliation":[{"name":"Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin 9054, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4942-4782","authenticated-orcid":false,"given":"Erwin","family":"Lamping","sequence":"additional","affiliation":[{"name":"Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin 9054, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8096-4245","authenticated-orcid":false,"given":"Alison M.","family":"Rich","sequence":"additional","affiliation":[{"name":"Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin 9054, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5398-2066","authenticated-orcid":false,"given":"Richard D.","family":"Cannon","sequence":"additional","affiliation":[{"name":"Sir John Walsh Research Institute, Faculty of Dentistry, University of Otago, Dunedin 9054, New Zealand"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,4,12]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1177\/10454411990100030701","article-title":"Oral colonization by Candida albicans","volume":"10","author":"Cannon","year":"1999","journal-title":"Crit. Rev. Oral Biol. Med."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"1069","DOI":"10.1016\/S0030-6665(20)30744-1","article-title":"Fungal infections of the oral cavity","volume":"26","author":"Zegarelli","year":"1993","journal-title":"Otolaryngol. Clin. N. Am."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"1152","DOI":"10.1177\/00220345950740050301","article-title":"Oral Candida: Clearance, colonization, or candidiasis?","volume":"74","author":"Cannon","year":"1995","journal-title":"J. Dent. Res."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"246","DOI":"10.1111\/j.1741-2358.2010.00407.x","article-title":"Prevalence and antifungal drug sensitivity of non-albicans Candida in oral rinse samples of self-caring elderly","volume":"28","author":"Meurman","year":"2011","journal-title":"Gerodontology"},{"key":"ref_5","unstructured":"Odds, F.C. (1988). Candida and Candidosis, Bailliere Tindall. [2nd ed.]."},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"178","DOI":"10.1016\/j.clindermatol.2009.12.014","article-title":"Candidosis, a new challenge","volume":"28","year":"2010","journal-title":"Clin. Dermatol."},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"142","DOI":"10.1016\/S1473-3099(10)70218-8","article-title":"Emerging opportunistic yeast infections","volume":"11","author":"Miceli","year":"2011","journal-title":"Lancet Infect. Dis."},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Lamont, R.J., Hajishengallis, G.N., and Jenkinson, H.F. (2014). Fungi and fungal infections of the oral cavity. Oral Microbiology and Immunology, ASM Press. [2nd ed.].","DOI":"10.1128\/9781555818906"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"71","DOI":"10.1146\/annurev-micro-091014-104330","article-title":"Candida albicans biofilms and human disease","volume":"69","author":"Nobile","year":"2015","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_10","first-page":"379","article-title":"Oral candidosis: Characterization of a sample of recurrent infections and study of resistance determinants","volume":"34","author":"Galle","year":"2011","journal-title":"New Microbiol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"2","DOI":"10.1111\/myc.12330","article-title":"Antifungal drug resistance among Candida species: Mechanisms and clinical impact","volume":"58","author":"Sanguinetti","year":"2015","journal-title":"Mycoses"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"541","DOI":"10.1080\/13693780801968571","article-title":"Candida lusitaniae fungemia in cancer patients: Risk factors for amphotericin B failure and outcome","volume":"46","author":"Atkinson","year":"2008","journal-title":"Med. Mycol."},{"key":"ref_13","doi-asserted-by":"crossref","unstructured":"Pinhati, H.M., Casulari, L.A., Souza, A.C., Siqueira, R.A., Damasceno, C.M., and Colombo, A.L. (2016). Outbreak of candidemia caused by fluconazole resistant Candida parapsilosis strains in an intensive care unit. BMC Infect. Dis., 16.","DOI":"10.1186\/s12879-016-1767-9"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"222","DOI":"10.1111\/j.1754-4505.1985.tb00577.x","article-title":"Oral candidiasis in the elderly","volume":"5","author":"Thomas","year":"1985","journal-title":"Spec. Care Dentist"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"667","DOI":"10.1111\/j.1601-0825.2012.01923.x","article-title":"Close association between oral Candida species and oral mucosal disorders in patients with xerostomia","volume":"18","author":"Shinozaki","year":"2012","journal-title":"Oral Dis."},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"316","DOI":"10.4103\/jomfp.JOMFP_231_16","article-title":"A relationship between salivary flow rates and Candida counts in patients with xerostomia","volume":"21","author":"Nadig","year":"2017","journal-title":"J. Oral Maxillofac Pathol."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"721","DOI":"10.1111\/odi.12337","article-title":"Oral mucosal lesions in older people: Relation to salivary secretion, systemic diseases and medications","volume":"21","author":"Nauntofte","year":"2015","journal-title":"Oral Dis."},{"key":"ref_18","unstructured":"World Health Organization (2018, December 20). Ageing and Health. Available online: https:\/\/www.who.int\/news-room\/fact-sheets\/detail\/ageing-and-health."},{"key":"ref_19","first-page":"1199","article-title":"Treatment of invasive candidiasis in the elderly: A review","volume":"8","author":"Flevari","year":"2013","journal-title":"Clin. Interv Aging"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.sdj.2014.10.001","article-title":"An ageing population poses dental challenges","volume":"35C","author":"Thomson","year":"2014","journal-title":"Singapore Dent. J."},{"key":"ref_21","unstructured":"Statistics New Zealand (2019, March 26). Available online: http:\/\/archive.stats.govt.nz\/Census\/2013-census\/profile-and-summary-reports\/outside-norm\/residential-old.aspx."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"2208","DOI":"10.3389\/fmicb.2018.02208","article-title":"Characterizing the Human Mycobiota: A Comparison of Small Subunit rRNA, ITS1, ITS2, and Large Subunit rRNA Genomic Targets","volume":"9","author":"Hoggard","year":"2018","journal-title":"Front. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1199","DOI":"10.1002\/yea.320091106","article-title":"Nucleotide sequence analysis of the 5.8S rDNA and adjacent ITS2 region of Candida albicans and related species","volume":"9","author":"Lott","year":"1993","journal-title":"Yeast"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"93","DOI":"10.1136\/pgmj.73.856.93","article-title":"Signs and symptoms in patients with salivary gland hypofunction","volume":"73","author":"Longman","year":"1997","journal-title":"Postgrad. Med. J."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/0003-9969(80)90147-8","article-title":"The prevalence and intra-oral distribution of Candida albicans in man","volume":"25","author":"Arendorf","year":"1980","journal-title":"Arch. Oral Biol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1177\/00220345930720060301","article-title":"Salivary yeasts, saliva, and oral mucosa in the elderly","volume":"72","author":"Narhi","year":"1993","journal-title":"J. Dent. Res."},{"key":"ref_27","first-page":"41","article-title":"The prevalence of Candida albicans populations in the mouths of complete denture wearers","volume":"21","year":"1998","journal-title":"New Microbiol."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1416","DOI":"10.1177\/00220345940730080401","article-title":"Flow rates of resting whole and stimulated parotid saliva in relation to age and gender","volume":"73","author":"Percival","year":"1994","journal-title":"J. Dent. Res."},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"1103","DOI":"10.1093\/jac\/dkx047","article-title":"Changing epidemiology of candidaemia in Australia","volume":"72","author":"Chapman","year":"2017","journal-title":"J. Antimicrob. Chemother."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"528","DOI":"10.1097\/QCO.0000000000000411","article-title":"Emerging multidrug-resistant Candida species","volume":"30","author":"Colombo","year":"2017","journal-title":"Curr. Opin. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"7715","DOI":"10.1128\/AAC.02204-15","article-title":"Acquired multidrug antifungal resistance in Candida lusitaniae during therapy","volume":"59","author":"Asner","year":"2015","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_32","first-page":"2173","article-title":"Azole antifungal resistance in Candida albicans and emerging non-albicans Candida species","volume":"7","author":"Whaley","year":"2016","journal-title":"Front. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"935","DOI":"10.2217\/fmb.10.51","article-title":"Mechanisms of antifungal drug resistance in Candida dubliniensis","volume":"5","author":"Coleman","year":"2010","journal-title":"Future Microbiol."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"390","DOI":"10.20471\/acc.2016.55.03.06","article-title":"Salivary flow rate, oral yeast colonization and dental status in institutionalized and non-institutionalized elderly","volume":"55","author":"Glazar","year":"2016","journal-title":"Acta Clin. Croat."},{"key":"ref_35","first-page":"673","article-title":"Multilocus sequence typing (MLST) analysis of Candida albicans isolates colonizing acrylic dentures before and after denture replacement","volume":"55","author":"Choo","year":"2017","journal-title":"Med. Mycol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"51","DOI":"10.2334\/josnusd.45.51","article-title":"Colonization of the oral cavity by Candida species: Risk factors in long-term geriatric care","volume":"45","author":"Grimoud","year":"2003","journal-title":"J. Oral Sci."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/5\/2\/30\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T12:44:51Z","timestamp":1760186691000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/5\/2\/30"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,4,12]]},"references-count":36,"journal-issue":{"issue":"2","published-online":{"date-parts":[[2019,6]]}},"alternative-id":["jof5020030"],"URL":"https:\/\/doi.org\/10.3390\/jof5020030","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,4,12]]}}}