{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,17]],"date-time":"2026-06-17T22:47:43Z","timestamp":1781736463095,"version":"3.54.5"},"reference-count":31,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T00:00:00Z","timestamp":1562025600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>More than 20 different species of Mucorales can be responsible for human mucormycosis. Accurate identification to the species level is important. The morphological identification of Mucorales is not reliable, and the currently recommended identification standard is the molecular technique of sequencing the internal transcribed spacer regions. Nevertheless, matrix-assisted laser desorption ionization time-of-flight mass spectrometry has been shown to be an accurate alternative for the identification of bacteria, yeasts, and even filamentous fungi. Therefore, 38 Mucorales isolates, belonging to 12 different species or varieties, mainly from international collections, including 10 type or neo-type strains previously identified by molecular methods, were used to evaluate the usefulness of matrix-assisted laser desorption ionization time-of-flight mass spectrometry for the identification of human pathogenic Mucorales to the species level. One to three reference strains for each species were used to create a database of main spectrum profiles, and the remaining isolates were used as test isolates. A minimum of 10 spectra was used to build the main spectrum profile of each database strain. Interspecies discrimination for all the isolates, including species belonging to the same genus, was possible. Twenty isolates belonging to five species were used to test the database accuracy, and were correctly identified to the species level with a log-score &gt;2. In summary, matrix-assisted laser desorption ionization time-of-flight mass spectrometry is a reliable and rapid method for the identification of most of the human pathogenic Mucorales to the species level.<\/jats:p>","DOI":"10.3390\/jof5030056","type":"journal-article","created":{"date-parts":[[2019,7,2]],"date-time":"2019-07-02T12:11:17Z","timestamp":1562069477000},"page":"56","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Identification of Mucorales by Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry"],"prefix":"10.3390","volume":"5","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0383-1865","authenticated-orcid":false,"given":"Patrick","family":"Schwarz","sequence":"first","affiliation":[{"name":"Department of Internal Medicine, Respiratory and Critical Care Medicine, University Hospital Marburg, D-35043 Marburg, Germany"},{"name":"Center for Invasive Mycoses and Antifungals, Philipps University Marburg, D-35037 Marburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Houssem","family":"Guedouar","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Facult\u00e9 de M\u00e9decine, AP-HP, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, F-75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Farah","family":"Laouiti","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Facult\u00e9 de M\u00e9decine, AP-HP, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, F-75015 Paris, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6001-3135","authenticated-orcid":false,"given":"Fr\u00e9d\u00e9ric","family":"Grenouillet","sequence":"additional","affiliation":[{"name":"Centre Hospitalier R\u00e9gional Universitaire, H\u00f4pital Jean Minjoz, S\u00e9rologies Parasitaires et Fongiques, F-25030 Besan\u00e7on, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2817-3830","authenticated-orcid":false,"given":"Eric","family":"Dannaoui","sequence":"additional","affiliation":[{"name":"Universit\u00e9 Paris Descartes, Facult\u00e9 de M\u00e9decine, AP-HP, H\u00f4pital Europ\u00e9en Georges Pompidou, Unit\u00e9 de Parasitologie-Mycologie, F-75015 Paris, France"},{"name":"Working Group Dynamyc, Facult\u00e9 de M\u00e9decine, H\u00f4pital Henri Mondor, F-94010 Cr\u00e9teil, France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2019,7,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"634","DOI":"10.1086\/432579","article-title":"Epidemiology and outcome of zygomycosis: A review of 929 reported cases","volume":"41","author":"Roden","year":"2005","journal-title":"Clin. 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