{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T09:17:12Z","timestamp":1778923032945,"version":"3.51.4"},"reference-count":46,"publisher":"MDPI AG","issue":"2","license":[{"start":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T00:00:00Z","timestamp":1592438400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001824","name":"Czech Science Foundation","doi-asserted-by":"publisher","award":["19-10907S"],"award-info":[{"award-number":["19-10907S"]}],"id":[{"id":"10.13039\/501100001824","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Ministry of Education, Youth and Sports of the Czech Republic","award":["LO1509"],"award-info":[{"award-number":["LO1509"]}]},{"name":"Internal grant agency of the Palacky University","award":["433103781"],"award-info":[{"award-number":["433103781"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Rhizopus spp. are the most common etiological agents of mucormycosis, causing over 90% mortality in disseminated infections. The diagnosis relies on histopathology, culture, and\/or polymerase chain reaction. For the first time, the glycosylation of rhizoferrin (RHF) was described in a Rhizopus microsporus clinical isolate by liquid chromatography and accurate tandem mass spectrometry. The fermentation broth lyophilizate contained 345.3 \u00b1 13.5, 1.2 \u00b1 0.03, and 0.03 \u00b1 0.002 mg\/g of RHF, imido-RHF, and bis-imido-RHF, respectively. Despite a considerable RHF secretion rate, we did not obtain conclusive RHF detection from a patient with disseminated mucormycosis caused by the same R. microsporus strain. We hypothesize that parallel antimycotic therapy, RHF biotransformation, and metabolism compromised the analysis. On the other hand, the full profile of posaconazole metabolites was retrieved by our in house software CycloBranch.<\/jats:p>","DOI":"10.3390\/jof6020089","type":"journal-article","created":{"date-parts":[[2020,6,18]],"date-time":"2020-06-18T12:21:46Z","timestamp":1592482906000},"page":"89","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Rhizoferrin Glycosylation in Rhizopus microsporus"],"prefix":"10.3390","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1122-1098","authenticated-orcid":false,"given":"Anton","family":"\u0160kr\u00edba","sequence":"first","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, 142 20 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7133-7044","authenticated-orcid":false,"given":"Rutuja Hiraji","family":"Patil","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, 142 20 Prague, Czech Republic"},{"name":"Department of Analytical Chemistry, Faculty of Science, Palack\u00fd University, 771 46 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Petr","family":"Hub\u00e1\u010dek","sequence":"additional","affiliation":[{"name":"Department of Medical Microbiology, 2nd Faculty of Medicine, Charles University and Motol University Hospital, 150 06 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Radim","family":"Dobi\u00e1\u0161","sequence":"additional","affiliation":[{"name":"Public Health Institute in Ostrava, 702 00 Ostrava, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea","family":"Palyzov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, 142 20 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Helena","family":"Mare\u0161ov\u00e1","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, 142 20 Prague, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tom\u00e1\u0161","family":"Pluh\u00e1\u010dek","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, 142 20 Prague, Czech Republic"},{"name":"Department of Analytical Chemistry, Faculty of Science, Palack\u00fd University, 771 46 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8614-7059","authenticated-orcid":false,"given":"Vladim\u00edr","family":"Havl\u00ed\u010dek","sequence":"additional","affiliation":[{"name":"Institute of Microbiology of the Czech Academy of Sciences, V\u00edde\u0148sk\u00e1 1083, 142 20 Prague, Czech Republic"},{"name":"Department of Analytical Chemistry, Faculty of Science, Palack\u00fd University, 771 46 Olomouc, Czech Republic"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,6,18]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"12","DOI":"10.1016\/j.fbr.2016.11.002","article-title":"Mucor: A Janus-faced fungal genus with human health impact and industrial applications","volume":"31","author":"Nodet","year":"2017","journal-title":"Fungal Biol. 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