{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,12]],"date-time":"2026-10-12T21:19:07Z","timestamp":1791839947087,"version":"4.3.4"},"reference-count":46,"publisher":"MDPI AG","issue":"3","license":[{"start":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T00:00:00Z","timestamp":1599004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2016\/08730-6"],"award-info":[{"award-number":["2016\/08730-6"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["420480\/2018-8 and 134424\/2016-6"],"award-info":[{"award-number":["420480\/2018-8 and 134424\/2016-6"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Paracoccidioidomycosis (PCM) is a granulomatous fungal disease caused by the dimorphic fungal species of Paracoccidioides, which mainly affects the lungs. Modern strategies for the treatment and\/or prevention of PCM are based on a Th1-type immune response, which is important for controlling the disease. One of the most studied candidates for a vaccine is the P10 peptide, derived from the 43 kDa glycoprotein of Paracoccidioides brasiliensis. In order to improve its immune modulatory effect, the P10 peptide was associated with a chitosan-conjugated nanoparticle. The nanoparticles presented 220 nm medium size, poly dispersion index (PDI) below 0.5, zeta potential of +20 mV and encapsulation efficiency around 90%. The nanoparticles\u2019 non-toxicity was verified by hemolytic test and cell viability using murine macrophages. The nanoparticles were stable and presented physicochemical characteristics desirable for biological applications, reducing the fungal load and the usual standard concentration of the peptide from 4 to 20 times.<\/jats:p>","DOI":"10.3390\/jof6030160","type":"journal-article","created":{"date-parts":[[2020,9,2]],"date-time":"2020-09-02T09:29:28Z","timestamp":1599038968000},"page":"160","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Intranasal Vaccine Using P10 Peptide Complexed within Chitosan Polymeric Nanoparticles as Experimental Therapy for Paracoccidioidomycosis in Murine Model"],"prefix":"10.3390","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5564-3538","authenticated-orcid":false,"given":"Samuel","family":"Rodrigues Dos Santos Junior","sequence":"first","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francenya","family":"Kelley Lopes da Silva","sequence":"additional","affiliation":[{"name":"Laboratorio de Nano &amp; Biotecnologia, Departamento de Biotecnologia, Instituto de Patologia Tropical e Sa\u00fade P\u00fablica, Universidade Federal de Goias, Goiania, GO 74605-050, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2344-1354","authenticated-orcid":false,"given":"Lucas","family":"Santos Dias","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9639-1207","authenticated-orcid":false,"given":"Ana Camila","family":"Oliveira Souza","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9695-3225","authenticated-orcid":false,"given":"Marcelo","family":"Valdemir de Araujo","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6545-5725","authenticated-orcid":false,"given":"Leandro","family":"Buffoni Roque da Silva","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP 05508-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Luiz R.","family":"Travassos","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de Sao Paulo, Sao Paulo, SP 04023-062, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andre","family":"Correa Amaral","sequence":"additional","affiliation":[{"name":"Laboratorio de Nano &amp; Biotecnologia, Departamento de Biotecnologia, Instituto de Patologia Tropical e Sa\u00fade P\u00fablica, Universidade Federal de Goias, Goiania, GO 74605-050, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9928-9809","authenticated-orcid":false,"given":"Carlos","family":"P. Taborda","sequence":"additional","affiliation":[{"name":"Departamento de Microbiologia, Instituto de Ci\u00eancias Biomedicas, Universidade de Sao Paulo, Sao Paulo, SP 05508-000, Brazil"},{"name":"Departamento de Dermatologia, Instituto de Medicina Tropical de Sao Paulo, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo, SP 05403-000, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,2]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1590\/S0036-46652015000700004","article-title":"Epidemiology of Paracoccidioidomycosis","volume":"57","author":"Martinez","year":"2015","journal-title":"Rev. Inst. Med. Trop. S\u00e3o Paulo"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Martinez, R. (2017). New Trends in Paracoccidioidomycosis Epidemiology. J. 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