{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,8]],"date-time":"2026-06-08T14:54:59Z","timestamp":1780930499965,"version":"3.54.1"},"reference-count":25,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T00:00:00Z","timestamp":1601251200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100005668","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Distrito Federal","doi-asserted-by":"publisher","award":["0193.001048\/2015"],"award-info":[{"award-number":["0193.001048\/2015"]}],"id":[{"id":"10.13039\/501100005668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005668","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Distrito Federal","doi-asserted-by":"publisher","award":["0193.001048\/2015"],"award-info":[{"award-number":["0193.001048\/2015"]}],"id":[{"id":"10.13039\/501100005668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100005668","name":"Funda\u00e7\u00e3o de Apoio \u00e0 Pesquisa do Distrito Federal","doi-asserted-by":"publisher","award":["193.001.533\/2016"],"award-info":[{"award-number":["193.001.533\/2016"]}],"id":[{"id":"10.13039\/501100005668","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","award":["437484\/2018-1"],"award-info":[{"award-number":["437484\/2018-1"]}],"id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001807","name":"Funda\u00e7\u00e3o de Amparo \u00e0 Pesquisa do Estado de S\u00e3o Paulo","doi-asserted-by":"publisher","award":["2015\/17178-2"],"award-info":[{"award-number":["2015\/17178-2"]}],"id":[{"id":"10.13039\/501100001807","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Paracoccidioidomycosis (PCM) is one of the most frequent systemic mycoses in Latin America. It affects mainly male rural workers in impoverished regions, and the therapy can last up to two years or use drugs that are very toxic. Given the need for novel safe and effective approaches to treat PCM, we have been developing monoclonal antibodies (mAbs) that could be used not only to block specific fungal targets, but also modulate the host\u2019s antifungal immunity. In this work we show the generation of and promising results with an mAb against Heat Shock Protein (HSP)90, a molecular chaperone that is an important virulence factor in fungi. Using recombinant Paracoccidioides lutzii (Pb01) and P. brasiliensis (Pb18) HSP90 proteins produced in E. coli, we immunized mice and generated polyclonal antibodies and an IgG1 hybridoma mAb. The proteins were very immunogenic and both the polyclonal serum and mAb were used in immunofluorescence experiments, which showed binding of antibodies to the yeast cell surface. The mAb successfully opsonized P. lutzii and P. brasiliensis cells in co-incubations with J774.16 macrophage-like cells. Our results suggest that this mAb could serve as the basis for new immunotherapy regimens for PCM.<\/jats:p>","DOI":"10.3390\/jof6040193","type":"journal-article","created":{"date-parts":[[2020,9,28]],"date-time":"2020-09-28T10:39:58Z","timestamp":1601289598000},"page":"193","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Paracoccidioides HSP90 Can Be Found in the Cell Surface and Is a Target for Antibodies with Therapeutic Potential"],"prefix":"10.3390","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0480-5028","authenticated-orcid":false,"given":"\u00c1gata","family":"Moura","sequence":"first","affiliation":[{"name":"Institute of Tropical Medicine, Department of Dermatology, Faculty of Medicine, University of S\u00e3o Paulo, S\u00e3o Paulo 05403-000, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2655-4672","authenticated-orcid":false,"given":"Diane","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, University of Bras\u00edlia, Bras\u00edlia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Verenice","family":"Paredes","sequence":"additional","affiliation":[{"name":"Karan Technologies Research and Development, Bras\u00edlia 70632-200, DF, Brazil"},{"name":"Faculty of Medicine, University of Bras\u00edlia, Bras\u00edlia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7832-058X","authenticated-orcid":false,"given":"Let\u00edcia","family":"Rocha","sequence":"additional","affiliation":[{"name":"Bacteriology Laboratory, Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiana","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Bras\u00edlia, Bras\u00edlia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gustavo","family":"Lessa","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Bras\u00edlia, Bras\u00edlia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9170-1034","authenticated-orcid":false,"given":"Juan","family":"Riasco-Palacios","sequence":"additional","affiliation":[{"name":"Faculty of Medicine, University of Bras\u00edlia, Bras\u00edlia 70910-900, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9402-9167","authenticated-orcid":false,"given":"Arturo","family":"Casadevall","sequence":"additional","affiliation":[{"name":"Department of Molecular Microbiology &amp; Immunology, Johns Hopkins Bloomberg School of Public Health, Baltimore, MD 21205, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8160-7784","authenticated-orcid":false,"given":"Patr\u00edcia","family":"Albuquerque","sequence":"additional","affiliation":[{"name":"Faculty of Ceil\u00e2ndia, University of Bras\u00edlia, Bras\u00edlia 72220-275, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria","family":"Felipe","sequence":"additional","affiliation":[{"name":"Institute of Biological Sciences, University of Bras\u00edlia, Bras\u00edlia 70910-900, DF, Brazil"},{"name":"Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Bras\u00edlia, Bras\u00edlia 70790-160, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roxane","family":"Piazza","sequence":"additional","affiliation":[{"name":"Bacteriology Laboratory, Butantan Institute, S\u00e3o Paulo 05503-900, SP, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8656-5835","authenticated-orcid":false,"given":"Andr\u00e9","family":"Nicola","sequence":"additional","affiliation":[{"name":"Karan Technologies Research and Development, Bras\u00edlia 70632-200, DF, Brazil"},{"name":"Faculty of Medicine, University of Bras\u00edlia, Bras\u00edlia 70910-900, DF, Brazil"},{"name":"Graduate Program in Genomic Sciences and Biotechnology, Catholic University of Bras\u00edlia, Bras\u00edlia 70790-160, DF, Brazil"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,9,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"11","DOI":"10.1590\/S0036-46652015000700004","article-title":"Epidemiology of paracoccidioidomycosis","volume":"57","author":"Martinez","year":"2015","journal-title":"Rev. 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