{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T20:44:22Z","timestamp":1791319462050,"version":"4.3.1"},"reference-count":41,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T00:00:00Z","timestamp":1603843200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"FUJIFILM Wako Chemicals Europe","award":["(no grant number)"],"award-info":[{"award-number":["(no grant number)"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The early diagnosis of invasive aspergillosis (IA) relies mainly on computed tomography imaging and testing for fungal biomarkers such as galactomannan (GM). We compared an established ELISA for the detection of GM with a turbidimetric assay for detection of the panfungal biomarker \u03b2-D-glucan (BDG) for early diagnosis of IA. A total of 226 serum specimens from 47 proven and seven probable IA cases were analysed. Sensitivity was calculated for samples obtained closest to the day of IA-diagnosis (d0). Additional analyses were performed by including samples obtained during the presumed course of disease. Most IA cases involved the respiratory system (63%), and Aspergillus fumigatus was the most frequently isolated species (59%). For proven cases, sensitivity of BDG\/GM analysis was 57%\/40%. Including all samples dating from \u20136 to +1 weeks from d0 increased sensitivities to 74%\/51%. Sensitivity of BDG testing was as high as or higher than GM testing for all subgroups and time intervals analysed. BDG testing was less specific (90\u201393%) than GM testing (99\u2013100%). Combining BDG and GM testing resulted in sensitivity\/specificity of 70%\/91%. Often, BDG testing was positive before GM testing. Our study backs the use of BDG for diagnosis of suspected IA. We suggest combining BDG and GM to improve the overall sensitivity.<\/jats:p>","DOI":"10.3390\/jof6040253","type":"journal-article","created":{"date-parts":[[2020,10,28]],"date-time":"2020-10-28T11:43:06Z","timestamp":1603885386000},"page":"253","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":23,"title":["Comparison of \u03b2-D-Glucan and Galactomannan in Serum for Detection of Invasive Aspergillosis: Retrospective Analysis with Focus on Early Diagnosis"],"prefix":"10.3390","volume":"6","author":[{"given":"Karl","family":"Dichtl","sequence":"first","affiliation":[{"name":"Max von Pettenkofer-Institut, Medizinische Fakult\u00e4t, LMU M\u00fcnchen, 80336 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8138-5891","authenticated-orcid":false,"given":"Johannes","family":"Forster","sequence":"additional","affiliation":[{"name":"Institut f\u00fcr Hygiene und Mikrobiologie, Julius-Maximilians-Universit\u00e4t W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Steffen","family":"Ormanns","sequence":"additional","affiliation":[{"name":"Pathologisches Institut, Medizinische Fakult\u00e4t, LMU M\u00fcnchen, 80337 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heidi","family":"Horns","sequence":"additional","affiliation":[{"name":"Klinik f\u00fcr Infektiologie und Tropenmedizin, M\u00fcnchen Klinik, 80804 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sebastian","family":"Suerbaum","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer-Institut, Medizinische Fakult\u00e4t, LMU M\u00fcnchen, 80336 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9830-8350","authenticated-orcid":false,"given":"Ulrich","family":"Seybold","sequence":"additional","affiliation":[{"name":"Sektion Klinische Infektiologie, Medizinische Klinik und Poliklinik IV, Klinikum der Universit\u00e4t, LMU Munich, 80336 Munich, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5952-9422","authenticated-orcid":false,"given":"Johannes","family":"Wagener","sequence":"additional","affiliation":[{"name":"Max von Pettenkofer-Institut, Medizinische Fakult\u00e4t, LMU M\u00fcnchen, 80336 Munich, Germany"},{"name":"Institut f\u00fcr Hygiene und Mikrobiologie, Julius-Maximilians-Universit\u00e4t W\u00fcrzburg, 97080 W\u00fcrzburg, Germany"},{"name":"Department of Clinical Microbiology, Trinity College Dublin, St James\u2019s Hospital Campus, D08 RX0X Dublin, Ireland"},{"name":"Nationales Referenzzentum f\u00fcr Invasive Pilzinfektionen (NRZMyk), 07745 Jena, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,10,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.idc.2015.10.004","article-title":"The Global Burden of Fungal Diseases","volume":"30","author":"Vallabhaneni","year":"2016","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1016\/j.cmi.2018.01.002","article-title":"Diagnosis and management of Aspergillus diseases: Executive summary of the 2017 ESCMID-ECMM-ERS guideline","volume":"24","author":"Ullmann","year":"2018","journal-title":"Clin. Microbiol. Infect."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"e1","DOI":"10.1093\/cid\/ciw326","article-title":"Practice Guidelines for the Diagnosis and Management of Aspergillosis: 2016 Update by the Infectious Diseases Society of America","volume":"63","author":"Patterson","year":"2016","journal-title":"Clin. Infect. Dis."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"577","DOI":"10.1086\/505870","article-title":"Comparison of epidemiological, clinical, and biological features of invasive aspergillosis in neutropenic and nonneutropenic patients: A 6-year survey","volume":"43","author":"Cornillet","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"ref_5","first-page":"R191","article-title":"Isolation of Aspergillus spp. from the respiratory tract in critically ill patients: Risk factors, clinical presentation and outcome","volume":"9","author":"Leon","year":"2005","journal-title":"Crit. Care"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"141","DOI":"10.1007\/s15010-013-0539-3","article-title":"SIMIFF study: Italian fungal registry of mold infections in hematological and non-hematological patients","volume":"42","author":"Montagna","year":"2014","journal-title":"Infection"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"782","DOI":"10.1016\/S2213-2600(18)30274-1","article-title":"Invasive aspergillosis in patients admitted to the intensive care unit with severe influenza: A retrospective cohort study","volume":"6","author":"Schauwvlieghe","year":"2018","journal-title":"Lancet Respir. Med."},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"156","DOI":"10.1183\/09059180.00001011","article-title":"Pulmonary aspergillosis: A clinical review","volume":"20","author":"Kousha","year":"2011","journal-title":"Eur. Respir. Rev."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"421","DOI":"10.1186\/s13054-015-1140-1","article-title":"Putative invasive pulmonary aspergillosis in critically ill patients with chronic obstructive pulmonary disease: A matched cohort study","volume":"19","author":"Delsuc","year":"2015","journal-title":"Crit. Care"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"82","DOI":"10.1093\/mmy\/myw114","article-title":"Invasive aspergillosis in acute myeloid leukemia: Are we making progress in reducing mortality?","volume":"55","author":"Dragonetti","year":"2017","journal-title":"Med. Mycol."},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"S14","DOI":"10.1016\/j.amjmed.2011.10.008","article-title":"Invasive mycoses: Diagnostic challenges","volume":"125","year":"2012","journal-title":"Am. J. Med."},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"121","DOI":"10.1016\/j.rmed.2018.06.029","article-title":"The spectrum of pulmonary aspergillosis","volume":"141","author":"Kanj","year":"2018","journal-title":"Respir. Med."},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1367","DOI":"10.1093\/cid\/ciz1008","article-title":"Revision and Update of the Consensus Definitions of Invasive Fungal Disease from the European Organization for Research and Treatment of Cancer and the Mycoses Study Group Education and Research Consortium","volume":"71","author":"Donnelly","year":"2019","journal-title":"Clin. Infect. Dis."},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"2237","DOI":"10.1128\/iai.60.6.2237-2245.1992","article-title":"Rat monoclonal antibodies against Aspergillus galactomannan","volume":"60","author":"Stynen","year":"1992","journal-title":"Infect. Immun."},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"1417","DOI":"10.1086\/503427","article-title":"Diagnosis of invasive aspergillosis using a galactomannan assay: A meta-analysis","volume":"42","author":"Pfeiffer","year":"2006","journal-title":"Clin. Infect. Dis."},{"key":"ref_16","first-page":"CD007394","article-title":"Galactomannan detection for invasive aspergillosis in immunocompromised patients","volume":"2015","author":"Leeflang","year":"2015","journal-title":"Cochrane Database Syst. Rev."},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"17","DOI":"10.1016\/S0140-6736(95)91152-9","article-title":"Plasma (1\u21923)-\u03b2-D-glucan measurement in diagnosis of invasive deep mycosis and fungal febrile episodes","volume":"345","author":"Obayashi","year":"1995","journal-title":"Lancet"},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Heitman, J., Howlett, B.J., Crous, P.W., Stukenbrock, E.H., James, T.Y., and Gow, N.A.R. (2018). The Fungal Cell Wall: Structure, Biosynthesis, and Function. The Fungal Kingdom, American Society of Microbiology.","DOI":"10.1128\/9781555819583"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"e12981","DOI":"10.1111\/tid.12981","article-title":"Incidence and outcome of invasive fungal diseases after allogeneic hematopoietic stem cell transplantation: A Swiss transplant cohort study","volume":"20","author":"Kuster","year":"2018","journal-title":"Transpl. Infect. Dis."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"654","DOI":"10.1086\/432470","article-title":"Multicenter clinical evaluation of the (1\u21923)-\u03b2-d-glucan assay as an aid to diagnosis of fungal infections in humans","volume":"41","author":"Alexander","year":"2005","journal-title":"Clin. Infect. Dis."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"1864","DOI":"10.1086\/588295","article-title":"Reappraisal of the serum (1,3)-\u03b2-d-glucan assay for the diagnosis of invasive fungal infections\u2014A study based on autopsy cases from 6 years","volume":"46","author":"Obayashi","year":"2008","journal-title":"Clin. Infect. Dis."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"1009","DOI":"10.1128\/JCM.02091-07","article-title":"Contribution of the (1\u21923)-\u03b2-d-glucan assay for diagnosis of invasive fungal infections","volume":"46","author":"Persat","year":"2008","journal-title":"J. Clin. Microbiol."},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"1650","DOI":"10.1086\/647942","article-title":"Diagnostic performance of the (1\u21923)-\u03b2-D-glucan assay for invasive fungal disease","volume":"49","author":"Koo","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"129","DOI":"10.1128\/JCM.00506-08","article-title":"Utility of galactomannan enzyme immunoassay and (1\u21923)-\u03b2-d-glucan in diagnosis of invasive fungal infections: Low sensitivity for Aspergillus fumigatus infection in hematologic malignancy patients","volume":"47","author":"Hachem","year":"2009","journal-title":"J. Clin. Microbiol."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"330","DOI":"10.3109\/00365548.2011.638319","article-title":"What should be the optimal cut-off of serum 1,3-\u03b2-d-glucan for the detection of invasive pulmonary aspergillosis in patients with haematological malignancies?","volume":"44","author":"Metan","year":"2012","journal-title":"Scand. J. Infect. Dis."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2328","DOI":"10.1128\/JCM.03567-13","article-title":"Use and limits of (1-3)-\u03b2-d-glucan assay (Fungitell), compared to galactomannan determination (Platelia Aspergillus), for diagnosis of invasive aspergillosis","volume":"52","author":"Sulahian","year":"2014","journal-title":"J. Clin. Microbiol."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"2433","DOI":"10.2169\/internalmedicine.53.2381","article-title":"Diagnostic value of the serum galactomannan and (1, 3)-\u03b2-d-glucan assays for invasive pulmonary aspergillosis in non-neutropenic patients","volume":"53","author":"Cai","year":"2014","journal-title":"Intern. Med."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"21484","DOI":"10.18632\/oncotarget.7471","article-title":"(1,3)-beta-d-glucan assay for diagnosing invasive fungal infections in critically ill patients with hematological malignancies","volume":"7","author":"Azoulay","year":"2016","journal-title":"Oncotarget"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"223","DOI":"10.1007\/s15010-015-0849-8","article-title":"Evaluation of serum (1\u21923)-\u03b2-d-glucan clinical performance: Kinetic assessment, comparison with galactomannan and evaluation of confounding factors","volume":"44","author":"Pini","year":"2016","journal-title":"Infection"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"843","DOI":"10.1093\/mmy\/myx004","article-title":"An evaluation of the performance of the Dynamiker\u00ae Fungus (1-3)-\u03b2-d-Glucan Assay to assist in the diagnosis of invasive aspergillosis, invasive candidiasis and Pneumocystis pneumonia","volume":"55","author":"White","year":"2017","journal-title":"Med. Mycol."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"576","DOI":"10.1111\/myc.12765","article-title":"Diagnostic value of serum galactomannan, (1,3)-\u03b2-d-glucan, and Aspergillus fumigatus-specific IgA and IgG assays for invasive pulmonary aspergillosis in non-neutropenic patients","volume":"61","author":"Dobias","year":"2018","journal-title":"Mycoses"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"e00464-18","DOI":"10.1128\/JCM.00464-18","article-title":"Comparative Analysis of the Wako beta-Glucan Test and the Fungitell Assay for Diagnosis of Candidemia and Pneumocystis jirovecii Pneumonia","volume":"56","author":"Friedrich","year":"2018","journal-title":"J. Clin. Microbiol."},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"473","DOI":"10.1007\/s10156-010-0198-6","article-title":"Clinical viability of Fungitell, a new (1\u21923)-\u03b2-d-glucan measurement kit, for diagnosis of invasive fungal infection, and comparison with other kits available in Japan","volume":"17","author":"Yoshida","year":"2011","journal-title":"J. Infect. Chemother."},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"3223","DOI":"10.1128\/JCM.37.10.3223-3228.1999","article-title":"Autopsy-controlled prospective evaluation of serial screening for circulating galactomannan by a sandwich enzyme-linked immunosorbent assay for hematological patients at risk for invasive Aspergillosis","volume":"37","author":"Maertens","year":"1999","journal-title":"J. Clin. Microbiol."},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1128\/JCM.38.1.438-443.2000","article-title":"Aspergillus fumigatus antigen detection in sera from patients at risk for invasive aspergillosis","volume":"38","author":"Chumpitazi","year":"2000","journal-title":"J. Clin. Microbiol."},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"1604","DOI":"10.1182\/blood.V97.6.1604","article-title":"Screening for circulating galactomannan as a noninvasive diagnostic tool for invasive aspergillosis in prolonged neutropenic patients and stem cell transplantation recipients: A prospective validation","volume":"97","author":"Maertens","year":"2001","journal-title":"Blood"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1086\/343804","article-title":"Use of circulating galactomannan screening for early diagnosis of invasive aspergillosis in allogeneic stem cell transplant recipients","volume":"186","author":"Maertens","year":"2002","journal-title":"J. Infect. Dis."},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"600","DOI":"10.3109\/00365548.2012.657672","article-title":"Diagnostic value of the serum galactomannan assay for invasive aspergillosis: It is less useful in non-haematological patients","volume":"44","author":"Ku","year":"2012","journal-title":"Scand. J. Infect. Dis."},{"key":"ref_39","doi-asserted-by":"crossref","first-page":"1696","DOI":"10.1093\/cid\/ciu673","article-title":"Serum galactomannan-based early detection of invasive aspergillosis in hematology patients receiving effective antimold prophylaxis","volume":"59","author":"Duarte","year":"2014","journal-title":"Clin. Infect. Dis."},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"145","DOI":"10.5603\/AIT.2014.0027","article-title":"Prognostic value of serum galactomannan in mixed ICU patients: A retrospective observational study","volume":"46","author":"Teering","year":"2014","journal-title":"Anaesthesiol. Intensive Ther."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"862","DOI":"10.1016\/j.cmi.2016.06.021","article-title":"Diagnosis of invasive fungal infections in haematological patients by combined use of galactomannan, 1,3-\u03b2-d-glucan, Aspergillus PCR, multifungal DNA-microarray, and Aspergillus azole resistance PCRs in blood and bronchoalveolar lavage samples: Results of a prospective multicentre study","volume":"22","author":"Boch","year":"2016","journal-title":"Clin. Microbiol. Infect."}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/6\/4\/253\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:29:36Z","timestamp":1760178576000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/6\/4\/253"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,10,28]]},"references-count":41,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,12]]}},"alternative-id":["jof6040253"],"URL":"https:\/\/doi.org\/10.3390\/jof6040253","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,10,28]]}}}