{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,20]],"date-time":"2026-05-20T04:03:33Z","timestamp":1779249813665,"version":"3.51.4"},"reference-count":86,"publisher":"MDPI AG","issue":"4","license":[{"start":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T00:00:00Z","timestamp":1606089600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Background: Systemic mycosis is a cause of death of immunocompromised subjects. The treatment directed to evade fungal pathogens shows severe limitations, such as time of drug exposure and side effects. The paracoccidioidomycosis (PCM) treatment depends on the severity of the infection and may last from months to years. Methods: To analyze the main interactions of Paracoccidioides lutzii isocitrate lyase (ICL) regarding the energetic metabolism through affinity chromatography, we performed blue native PAGE and co-immunoprecipitation to identify ICL interactions. We also performed in silico analysis by homology, docking, hot-spot prediction and contact preference analysis to identify the conformation of ICL complexes. Results: ICL interacted with 18 proteins in mycelium, 19 in mycelium-to-yeast transition, and 70 in yeast cells. Thirty complexes were predicted through docking and contact preference analysis. ICL has seven main regions of interaction with protein partners. Conclusions: ICL seems to interfere with energetic metabolism of P. lutzii, regulating aerobic and anaerobic metabolism as it interacts with proteins from glycolysis, gluconeogenesis, TCA and methylcitrate cycles, mainly through seven hot-spot residues.<\/jats:p>","DOI":"10.3390\/jof6040309","type":"journal-article","created":{"date-parts":[[2020,11,23]],"date-time":"2020-11-23T08:18:23Z","timestamp":1606119503000},"page":"309","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Interaction of Isocitrate Lyase with Proteins Involved in the Energetic Metabolism in Paracoccidioides lutzii"],"prefix":"10.3390","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-5350-7313","authenticated-orcid":false,"given":"Kleber Santiago","family":"Freitas e Silva","sequence":"first","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1210-8302","authenticated-orcid":false,"given":"Raisa","family":"Melo Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8784-8167","authenticated-orcid":false,"given":"Patr\u00edcia","family":"de Sousa Lima","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lilian","family":"Cristiane Baeza","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Goi\u00e1s, Brazil"},{"name":"Laborat\u00f3rio de Microbiologia Experimental, Centro de Ci\u00eancias M\u00e9dicas e Farmac\u00eauticas, Universidade Estadual do Oeste do Paran\u00e1, Cascavel 85819-170, Paran\u00e1, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5624-4790","authenticated-orcid":false,"given":"Roosevelt","family":"Alves da Silva","sequence":"additional","affiliation":[{"name":"N\u00facleo Colaborativo de Biossistemas, Instituto de Ci\u00eancias Exatas, Universidade Federal de Jata\u00ed, Jata\u00ed 75801-615, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5378-9840","authenticated-orcid":false,"given":"C\u00e9lia","family":"Maria de Almeida Soares","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maristela","family":"Pereira","sequence":"additional","affiliation":[{"name":"Laborat\u00f3rio de Biologia Molecular, Instituto de Ci\u00eancias Biol\u00f3gicas, Universidade Federal de Goi\u00e1s, Goi\u00e2nia 74690-900, Goi\u00e1s, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2020,11,23]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"e00213-16","DOI":"10.1128\/mSphere.00213-16","article-title":"Genome Diversity, Recombination, and Virulence across the Major Lineages of Paracoccidioides","volume":"1","author":"Farrer","year":"2016","journal-title":"mSphere"},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1016\/j.fgb.2017.05.007","article-title":"Species boundaries in the human pathogen Paracoccidioides","volume":"106","author":"Turissini","year":"2017","journal-title":"Fungal Genet. 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