{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T01:10:53Z","timestamp":1787447453576,"version":"build-2736575974"},"reference-count":69,"publisher":"MDPI AG","issue":"1","license":[{"start":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T00:00:00Z","timestamp":1608854400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"National Instititues of Health","award":["1R01AI127704- 01A1"],"award-info":[{"award-number":["1R01AI127704- 01A1"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Candida albicans, Candida auris, Candida glabrata, and Cryptococcus neoformans are pathogenic yeasts which can cause systemic infections in immune-compromised as well as immune-competent individuals. These yeasts undergo replicative aging analogous to a process first described in the nonpathogenic yeast Saccharomyces cerevisiae. The hallmark of replicative aging is the asymmetric cell division of mother yeast cells that leads to the production of a phenotypically distinct daughter cell. Several techniques to study aging that have been pioneered in S. cerevisiae have been adapted to study aging in other pathogenic yeasts. The studies indicate that aging is relevant for virulence in pathogenic fungi. As the mother yeast cell progressively ages, every ensuing asymmetric cell division leads to striking phenotypic changes, which results in increased antifungal and antiphagocytic resistance. This review summarizes the various techniques that are used to study replicative aging in pathogenic fungi along with their limitations. Additionally, the review summarizes some key phenotypic variations that have been identified and are associated with changes in virulence or resistance and thus promote persistence of older cells.<\/jats:p>","DOI":"10.3390\/jof7010006","type":"journal-article","created":{"date-parts":[[2020,12,25]],"date-time":"2020-12-25T09:30:19Z","timestamp":1608888619000},"page":"6","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":19,"title":["Replicative Aging in Pathogenic Fungi"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8562-3877","authenticated-orcid":false,"given":"Somanon","family":"Bhattacharya","sequence":"first","affiliation":[{"name":"Department of Medicine, Stony Brook University, Stony Brook, NY 11794, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tejas","family":"Bouklas","sequence":"additional","affiliation":[{"name":"Department of Medicine, Stony Brook University, Stony Brook, NY 11794, USA"},{"name":"Department of Biological Sciences, State University of New York College at Old Westbury, Old Westbury, NY 11568, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bettina C.","family":"Fries","sequence":"additional","affiliation":[{"name":"Department of Medicine, Stony Brook University, Stony Brook, NY 11794, USA"},{"name":"Department of Microbiology and Immunology, Stony Brook University, Stony Brook, NY 11794, USA"},{"name":"Veterans Administration Medical Center, Northport, NY 11768, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2020,12,25]]},"reference":[{"key":"ref_1","first-page":"e1209","article-title":"Measuring replicative life span in the budding yeast","volume":"28","author":"Steffen","year":"2009","journal-title":"J. Vis. Exp."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"2690","DOI":"10.1016\/j.bbadis.2018.02.023","article-title":"The yeast replicative aging model","volume":"1864","author":"He","year":"2018","journal-title":"Biochim. Biophys. Acta. Mol. Basis Dis."},{"key":"ref_3","doi-asserted-by":"crossref","first-page":"13220","DOI":"10.1038\/s41598-017-13576-w","article-title":"The contribution of Saccharomyces cerevisiae replicative age to the variations in the levels of Trx2p, Pdr5p, Can1p and Idh isoforms","volume":"7","author":"Azbarova","year":"2017","journal-title":"Sci. Rep."},{"key":"ref_4","doi-asserted-by":"crossref","first-page":"591","DOI":"10.1111\/gtc.12497","article-title":"Proteomics analysis for asymmetric inheritance of preexisting proteins between mother and daughter cells in budding yeast","volume":"22","author":"Okada","year":"2017","journal-title":"Genes Cells Devoted Mol. Cell. Mech."},{"key":"ref_5","doi-asserted-by":"crossref","first-page":"e41046","DOI":"10.7554\/eLife.41046","article-title":"Saccharomyces cerevisiae goes through distinct metabolic phases during its replicative lifespan","volume":"8","author":"Leupold","year":"2019","journal-title":"Elife"},{"key":"ref_6","doi-asserted-by":"crossref","unstructured":"Bouklas, T., Pechuan, X., Goldman, D.L., Edelman, B., Bergman, A., and Fries, B.C. (2013). Old Cryptococcus neoformans cells contribute to virulence in chronic cryptococcosis. mBio, 4.","DOI":"10.1128\/mBio.00455-13"},{"key":"ref_7","doi-asserted-by":"crossref","first-page":"e42976","DOI":"10.7554\/eLife.42976","article-title":"Advancing the aging biology toolkit","volume":"7","author":"Coody","year":"2018","journal-title":"Elife"},{"key":"ref_8","doi-asserted-by":"crossref","first-page":"256","DOI":"10.1038\/s42003-019-0504-5","article-title":"High-Throughput Yeast Aging Analysis for Cryptococcus (HYAAC) microfluidic device streamlines aging studies in Cryptococcus neoformans","volume":"2","author":"Orner","year":"2019","journal-title":"Commun. Biol."},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"151","DOI":"10.1016\/j.tma.2019.09.002","article-title":"Advances in quantitative biology methods for studying replicative aging in Saccharomyces cerevisiae","volume":"4","author":"Jin","year":"2020","journal-title":"Transl. Med. Aging"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"262","DOI":"10.1016\/j.mad.2016.03.009","article-title":"Microfluidic technologies for yeast replicative lifespan studies","volume":"161","author":"Chen","year":"2017","journal-title":"Mech. Ageing Dev."},{"key":"ref_11","doi-asserted-by":"crossref","unstructured":"Zhang, Y., Luo, C., Zou, K., Xie, Z., Brandman, O., Ouyang, Q., and Li, H. (2012). Single cell analysis of yeast replicative aging using a new generation of microfluidic device. PLoS ONE, 7.","DOI":"10.1371\/journal.pone.0048275"},{"key":"ref_12","doi-asserted-by":"crossref","unstructured":"Crane, M.M., Clark, I.B., Bakker, E., Smith, S., and Swain, P.S. (2014). A microfluidic system for studying ageing and dynamic single-cell responses in budding yeast. PLoS ONE, 9.","DOI":"10.1371\/journal.pone.0100042"},{"key":"ref_13","doi-asserted-by":"crossref","first-page":"1065","DOI":"10.1002\/yea.1412","article-title":"Microfluidics device for single cell gene expression analysis in Saccharomyces cerevisiae","volume":"23","author":"Ryley","year":"2006","journal-title":"Yeast"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"9364","DOI":"10.1073\/pnas.1510328112","article-title":"High-throughput analysis of yeast replicative aging using a microfluidic system","volume":"112","author":"Jo","year":"2015","journal-title":"Proc. Natl. Acad. Sci. USA"},{"key":"ref_15","doi-asserted-by":"crossref","first-page":"eaao4666","DOI":"10.1126\/sciadv.aao4666","article-title":"The generational scalability of single-cell replicative aging","volume":"4","author":"Liu","year":"2018","journal-title":"Sci. Adv."},{"key":"ref_16","doi-asserted-by":"crossref","unstructured":"Bhattacharya, S., and Fries, B.C. (2018). Enhanced Efflux Pump Activity in Old Candida glabrata Cells. Antimicrob. Agents Chemother., 62.","DOI":"10.1128\/AAC.02227-17"},{"key":"ref_17","doi-asserted-by":"crossref","first-page":"5052","DOI":"10.1038\/s41598-019-41513-6","article-title":"Gene Duplication Associated with Increased Fluconazole Tolerance in Candida auris cells of Advanced Generational Age","volume":"9","author":"Bhattacharya","year":"2019","journal-title":"Sci. Rep."},{"key":"ref_18","doi-asserted-by":"crossref","unstructured":"Bouklas, T., Alonso-Crisostomo, L., Szekely, T., Diago-Navarro, E., Orner, E.P., Smith, K., Munshi, M.A., Del Poeta, M., Balazsi, G., and Fries, B.C. (2017). Generational distribution of a Candida glabrata population: Resilient old cells prevail, while younger cells dominate in the vulnerable host. PLoS Pathog., 13.","DOI":"10.1371\/journal.ppat.1006355"},{"key":"ref_19","doi-asserted-by":"crossref","first-page":"533","DOI":"10.1146\/annurev.micro.52.1.533","article-title":"Aging in Saccharomyces cerevisiae","volume":"52","author":"Sinclair","year":"1998","journal-title":"Annu. Rev. Microbiol."},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"746","DOI":"10.1111\/j.1474-9726.2008.00424.x","article-title":"Candida albicans, a distinctive fungal model for cellular aging study","volume":"7","author":"Fu","year":"2008","journal-title":"Aging Cell"},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"858","DOI":"10.1128\/EC.00017-09","article-title":"Isolation and characterization of senescent Cryptococcus neoformans and implications for phenotypic switching and pathogenesis in chronic cryptococcosis","volume":"8","author":"Jain","year":"2009","journal-title":"Eukaryot. Cell"},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1002\/yea.320110409","article-title":"Production of senescent cells of Saccharomyces cerevisiae by centrifugal elutriation","volume":"11","author":"Woldringh","year":"1995","journal-title":"Yeast"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1534\/genetics.109.106229","article-title":"The mother enrichment program: A genetic system for facile replicative life span analysis in Saccharomyces cerevisiae","volume":"183","author":"Lindstrom","year":"2009","journal-title":"Genetics"},{"key":"ref_24","doi-asserted-by":"crossref","first-page":"1579","DOI":"10.1534\/g3.118.200161","article-title":"Rapid Nuclear Exclusion of Hcm1 in Aging Saccharomyces cerevisiae Leads to Vacuolar Alkalization and Replicative Senescence","volume":"8","author":"Ghavidel","year":"2018","journal-title":"Genes Genomes Genet."},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"15211","DOI":"10.1038\/s41598-020-72167-4","article-title":"Age-dependent decline in stress response capacity revealed by proteins dynamics analysis","volume":"10","author":"Chen","year":"2020","journal-title":"Sci. Rep."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"2639","DOI":"10.1016\/j.celrep.2017.11.002","article-title":"A High-Throughput Screen for Yeast Replicative Lifespan Identifies Lifespan-Extending Compounds","volume":"21","author":"Sarnoski","year":"2017","journal-title":"Cell Rep."},{"key":"ref_27","doi-asserted-by":"crossref","first-page":"eaaq0236","DOI":"10.1126\/sciadv.aaq0236","article-title":"Impaired cohesion and homologous recombination during replicative aging in budding yeast","volume":"4","author":"Pal","year":"2018","journal-title":"Sci. Adv."},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"e03504","DOI":"10.7554\/eLife.03504","article-title":"Mother-daughter asymmetry of pH underlies aging and rejuvenation in yeast","volume":"3","author":"Henderson","year":"2014","journal-title":"Elife"},{"key":"ref_29","doi-asserted-by":"crossref","first-page":"2513","DOI":"10.3389\/fmicb.2019.02513","article-title":"Cell Wall-Associated Virulence Factors Contribute to Increased Resilience of Old Cryptococcus neoformans Cells","volume":"10","author":"Orner","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"361","DOI":"10.1242\/jcs.50.1.361","article-title":"Variability in individual cell cycles of Saccharomyces cerevisiae","volume":"50","author":"Lord","year":"1981","journal-title":"J. Cell Sci."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"263","DOI":"10.15698\/mic2016.07.510","article-title":"Evidence for the hallmarks of human aging in replicatively aging yeast","volume":"3","author":"Janssens","year":"2016","journal-title":"Microb. Cell"},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"37","DOI":"10.15698\/mic2014.01.122","article-title":"Early manifestations of replicative aging in the yeast Saccharomyces cerevisiae","volume":"1","author":"Sorokin","year":"2014","journal-title":"Microb. Cell"},{"key":"ref_33","doi-asserted-by":"crossref","first-page":"481","DOI":"10.1007\/s10522-008-9192-0","article-title":"Cell volume as a factor limiting the replicative lifespan of the yeast Saccharomyces cerevisiae","volume":"10","author":"Bartosz","year":"2009","journal-title":"Biogerontology"},{"key":"ref_34","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1007\/s00792-005-0431-x","article-title":"Cells of Pseudomonas putida and Enterobacter sp. adapt to toxic organic compounds by increasing their size","volume":"9","author":"Neumann","year":"2005","journal-title":"Extremophiles"},{"key":"ref_35","doi-asserted-by":"crossref","first-page":"100","DOI":"10.1126\/science.1216166","article-title":"Asymmetry and aging of mycobacterial cells lead to variable growth and antibiotic susceptibility","volume":"335","author":"Aldridge","year":"2012","journal-title":"Science"},{"key":"ref_36","doi-asserted-by":"crossref","first-page":"4501","DOI":"10.4161\/cc.9.22.13683","article-title":"Sir2-dependent daughter-to-mother transport of the damaged proteins in yeast is required to prevent high stress sensitivity of the daughters","volume":"9","author":"Knorre","year":"2010","journal-title":"Cell Cycle"},{"key":"ref_37","doi-asserted-by":"crossref","first-page":"1089","DOI":"10.1111\/j.1474-9726.2011.00742.x","article-title":"Sir2 deletion prevents lifespan extension in 32 long-lived mutants","volume":"10","author":"Delaney","year":"2011","journal-title":"Aging Cell"},{"key":"ref_38","doi-asserted-by":"crossref","first-page":"144","DOI":"10.4161\/cc.10.1.14455","article-title":"Cell size and growth rate are major determinants of replicative lifespan","volume":"10","author":"Yang","year":"2011","journal-title":"Cell Cycle"},{"key":"ref_39","doi-asserted-by":"crossref","unstructured":"Bouklas, T., Masone, L., and Fries, B.C. (2018). Differences in Sirtuin Regulation in Response to Calorie Restriction in Cryptococcus neoformans. J. Fungi, 4.","DOI":"10.3390\/jof4010026"},{"key":"ref_40","doi-asserted-by":"crossref","first-page":"98","DOI":"10.3389\/fmicb.2017.00098","article-title":"Modulation of Replicative Lifespan in Cryptococcus neoformans: Implications for Virulence","volume":"8","author":"Bouklas","year":"2017","journal-title":"Front. Microbiol."},{"key":"ref_41","doi-asserted-by":"crossref","first-page":"1730","DOI":"10.1111\/j.1462-5822.2006.00742.x","article-title":"Characterization of a flocculation-like phenotype in Cryptococcus neoformans and its effects on pathogenesis","volume":"8","author":"Li","year":"2006","journal-title":"Cell Microbiol."},{"key":"ref_42","doi-asserted-by":"crossref","first-page":"2355","DOI":"10.1099\/00221287-147-8-2355","article-title":"Dynamic changes in the morphology of Cryptococcus neoformans during murine pulmonary infection","volume":"147","author":"Feldmesser","year":"2001","journal-title":"Microbiology"},{"key":"ref_43","doi-asserted-by":"crossref","first-page":"S115","DOI":"10.1093\/ofid\/ofx163.133","article-title":"Replicative Aging in Candida auris Has an Effect on Antifungal Resistance","volume":"Volume 4","author":"Fries","year":"2017","journal-title":"Open Forum Infectious Diseases, US"},{"key":"ref_44","doi-asserted-by":"crossref","first-page":"737","DOI":"10.1038\/nrmicro2636","article-title":"Growth of Candida albicans hyphae","volume":"9","author":"Sudbery","year":"2011","journal-title":"Nat. Rev. Microbiol."},{"key":"ref_45","first-page":"2993","article-title":"The Fungal Cell Wall: Candida, Cryptococcus, and Aspergillus Species","volume":"10","author":"Rivera","year":"2019","journal-title":"Front. Microbiol."},{"key":"ref_46","doi-asserted-by":"crossref","first-page":"3129","DOI":"10.1099\/mic.0.25940-0","article-title":"Chitin scar breaks in aged Saccharomyces cerevisiae","volume":"149","author":"Powell","year":"2003","journal-title":"Microbiology"},{"key":"ref_47","doi-asserted-by":"crossref","first-page":"67","DOI":"10.1111\/j.1365-2958.2005.04928.x","article-title":"The polysaccharide capsule of the pathogenic fungus Cryptococcus neoformans enlarges by distal growth and is rearranged during budding","volume":"59","author":"Zaragoza","year":"2006","journal-title":"Mol. Microbiol."},{"key":"ref_48","doi-asserted-by":"crossref","first-page":"497","DOI":"10.1016\/j.tim.2006.09.003","article-title":"The capsular dynamics of Cryptococcus neoformans","volume":"14","author":"McFadden","year":"2006","journal-title":"Trends Microbiol."},{"key":"ref_49","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1046\/j.1365-2958.2002.03166.x","article-title":"Modular domain structure in the Candida glabrata adhesin Epa1p, a beta1,6 glucan-cross-linked cell wall protein","volume":"46","author":"Frieman","year":"2002","journal-title":"Mol. Microbiol."},{"key":"ref_50","doi-asserted-by":"crossref","first-page":"578","DOI":"10.1126\/science.285.5427.578","article-title":"An adhesin of the yeast pathogen Candida glabrata mediating adherence to human epithelial cells","volume":"285","author":"Cormack","year":"1999","journal-title":"Science"},{"key":"ref_51","doi-asserted-by":"crossref","first-page":"1006","DOI":"10.1016\/j.exger.2012.12.001","article-title":"Dietary restriction and mitochondrial function link replicative and chronological aging in Saccharomyces cerevisiae","volume":"48","author":"Delaney","year":"2013","journal-title":"Exp. Gerontol."},{"key":"ref_52","doi-asserted-by":"crossref","first-page":"885","DOI":"10.1111\/j.1474-9726.2011.00731.x","article-title":"Mitochondrial quality control during inheritance is associated with lifespan and mother-daughter age asymmetry in budding yeast","volume":"10","author":"Vevea","year":"2011","journal-title":"Aging Cell"},{"key":"ref_53","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1038\/nature11654","article-title":"An early age increase in vacuolar pH limits mitochondrial function and lifespan in yeast","volume":"492","author":"Hughes","year":"2012","journal-title":"Nature"},{"key":"ref_54","doi-asserted-by":"crossref","first-page":"1751","DOI":"10.1126\/science.1080418","article-title":"Asymmetric inheritance of oxidatively damaged proteins during cytokinesis","volume":"299","author":"Aguilaniu","year":"2003","journal-title":"Science"},{"key":"ref_55","doi-asserted-by":"crossref","first-page":"2410","DOI":"10.1101\/gad.439307","article-title":"Accelerated aging and failure to segregate damaged proteins in Sir2 mutants can be suppressed by overproducing the protein aggregation-remodeling factor Hsp104p","volume":"21","author":"Erjavec","year":"2007","journal-title":"Genes Dev."},{"key":"ref_56","doi-asserted-by":"crossref","first-page":"36000","DOI":"10.1074\/jbc.M103509200","article-title":"Enhanced gluconeogenesis and increased energy storage as hallmarks of aging in Saccharomyces cerevisiae","volume":"276","author":"Lin","year":"2001","journal-title":"J. Biol. Chem."},{"key":"ref_57","doi-asserted-by":"crossref","first-page":"1297","DOI":"10.1091\/mbc.e03-10-0742","article-title":"The transcriptome of prematurely aging yeast cells is similar to that of telomerase-deficient cells","volume":"15","author":"Lesur","year":"2004","journal-title":"Mol. Biol. Cell"},{"key":"ref_58","doi-asserted-by":"crossref","first-page":"813","DOI":"10.1016\/j.exger.2006.06.049","article-title":"Accumulation of oxidative damage during replicative aging of the yeast Saccharomyces cerevisiae","volume":"41","author":"Grzelak","year":"2006","journal-title":"Exp. Gerontol."},{"key":"ref_59","doi-asserted-by":"crossref","first-page":"5124","DOI":"10.1128\/IAI.70.9.5124-5131.2002","article-title":"Histoplasma capsulatum synthesizes melanin-like pigments in vitro and during mammalian infection","volume":"70","author":"Nosanchuk","year":"2002","journal-title":"Infect. Immun."},{"key":"ref_60","doi-asserted-by":"crossref","first-page":"2049","DOI":"10.1016\/S0140-6736(00)02356-4","article-title":"Melanisation of Cryptococcus neoformans in human brain tissue","volume":"355","author":"Nosanchuk","year":"2000","journal-title":"Lancet"},{"key":"ref_61","doi-asserted-by":"crossref","first-page":"291","DOI":"10.1099\/mic.0.27433-0","article-title":"Melanization of Penicillium marneffei in vitro and in vivo","volume":"151","author":"Youngchim","year":"2005","journal-title":"Microbiology"},{"key":"ref_62","doi-asserted-by":"crossref","first-page":"175","DOI":"10.1099\/jmm.0.05421-0","article-title":"Production of melanin by Aspergillus fumigatus","volume":"53","author":"Youngchim","year":"2004","journal-title":"J. Med. Microbiol."},{"key":"ref_63","doi-asserted-by":"crossref","first-page":"203","DOI":"10.1046\/j.1462-5814.2003.00268.x","article-title":"The contribution of melanin to microbial pathogenesis","volume":"5","author":"Nosanchuk","year":"2003","journal-title":"Cell Microbiol."},{"key":"ref_64","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1016\/j.femsyr.2004.04.004","article-title":"Role of laccase in the biology and virulence of Cryptococcus neoformans","volume":"5","author":"Zhu","year":"2004","journal-title":"FEMS Yeast Res."},{"key":"ref_65","doi-asserted-by":"crossref","first-page":"3131","DOI":"10.1128\/iai.63.8.3131-3136.1995","article-title":"Cryptococcus neoformans melanin and virulence: Mechanism of action","volume":"63","author":"Wang","year":"1995","journal-title":"Infect. Immun."},{"key":"ref_66","doi-asserted-by":"crossref","first-page":"3394","DOI":"10.1128\/AAC.46.11.3394-3400.2002","article-title":"Melanization of Cryptococcus neoformans and Histoplasma capsulatum reduces their susceptibilities to amphotericin B and caspofungin","volume":"46","author":"Casadevall","year":"2002","journal-title":"Antimicrob. Agents Chemother."},{"key":"ref_67","doi-asserted-by":"crossref","first-page":"1945","DOI":"10.1099\/mic.0.26333-0","article-title":"Budding of melanized Cryptococcus neoformans in the presence or absence of L-dopa","volume":"149","author":"Nosanchuk","year":"2003","journal-title":"Microbiology"},{"key":"ref_68","doi-asserted-by":"crossref","first-page":"15083","DOI":"10.1074\/jbc.RA120.015201","article-title":"Solid-state NMR spectroscopy identifies three classes of lipids in Cryptococcus neoformans melanized cell walls and whole fungal cells","volume":"295","author":"Chrissian","year":"2020","journal-title":"J. Biol. Chem."},{"key":"ref_69","doi-asserted-by":"crossref","unstructured":"Bhattacharya, S., Sae-Tia, S., and Fries, B.C. (2020). Candidiasis and Mechanisms of Antifungal Resistance. Antibiotics, 9.","DOI":"10.3390\/antibiotics9060312"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/7\/1\/6\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T10:46:05Z","timestamp":1760179565000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/7\/1\/6"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,12,25]]},"references-count":69,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,1]]}},"alternative-id":["jof7010006"],"URL":"https:\/\/doi.org\/10.3390\/jof7010006","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2020,12,25]]}}}