{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,29]],"date-time":"2026-04-29T02:47:34Z","timestamp":1777430854872,"version":"3.51.4"},"reference-count":33,"publisher":"MDPI AG","issue":"5","license":[{"start":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T00:00:00Z","timestamp":1619568000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>The acute form of histoplasmosis usually occurs after the exposition of more than one individual to a common environmental source harboring Histoplasma capsulatum. Here, we present two cases of acute pulmonary histoplasmosis seen within two weeks at a reference center for infectious diseases at Rio de Janeiro, Brazil. The patients did not present a common epidemiologic history for histoplasmosis, however both presented COVID-19 before the onset of histoplasmosis symptoms. Due to the difficulties in the diagnosis of acute histoplasmosis, novel laboratory methods such as Western Blot and PCR were included in the investigation of these cases. Both patients presented negative cultures for H. capsulatum and negative urinary galactomannan. However, they presented H and M bands in the Western blot as well as a positive H. capsulatum DNA detection in sputum. These results were available approximately 36 h after sample collection, fastening the beginning of treatment of one patient. Both patients progressed well with itraconazole treatment. These cases suggest that COVID-19 may facilitate the development of acute pulmonary histoplasmosis and, therefore, clinicians must be aware of this differential diagnosis in patients from endemic areas with fever and coughing after recovery from COVID-19.<\/jats:p>","DOI":"10.3390\/jof7050346","type":"journal-article","created":{"date-parts":[[2021,4,28]],"date-time":"2021-04-28T10:41:58Z","timestamp":1619606518000},"page":"346","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Acute Pulmonary Histoplasmosis Following COVID-19: Novel Laboratorial Methods Aiding Diagnosis"],"prefix":"10.3390","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-6900-9703","authenticated-orcid":false,"given":"Priscila Marques","family":"de Macedo","sequence":"first","affiliation":[{"name":"Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Andrea D\u2019\u00c1vila","family":"Freitas","sequence":"additional","affiliation":[{"name":"Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thiago Prudente","family":"B\u00e1rtholo","sequence":"additional","affiliation":[{"name":"Pulmonology and Tisiology Service, Pedro Ernesto University Hospital, University of the State of Rio de Janeiro, Maracan\u00e3, Rio de Janeiro 20550-013, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4997-8734","authenticated-orcid":false,"given":"Andrea Reis","family":"Bernardes-Engemann","sequence":"additional","affiliation":[{"name":"Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5581-631X","authenticated-orcid":false,"given":"Marcos de Abreu","family":"Almeida","sequence":"additional","affiliation":[{"name":"Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fernando","family":"Almeida-Silva","sequence":"additional","affiliation":[{"name":"Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rosely Maria","family":"Zancop\u00e9-Oliveira","sequence":"additional","affiliation":[{"name":"Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6639-6058","authenticated-orcid":false,"given":"Rodrigo","family":"Almeida-Paes","sequence":"additional","affiliation":[{"name":"Evandro Chagas National Institute of Infectious Diseases, Fiocruz, Rio de Janeiro 21040-900, Brazil"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"1968","published-online":{"date-parts":[[2021,4,28]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","first-page":"207","DOI":"10.1016\/j.idc.2015.10.009","article-title":"Histoplasmosis","volume":"30","author":"Wheat","year":"2016","journal-title":"Infect. Dis. Clin. N. Am."},{"key":"ref_2","doi-asserted-by":"crossref","first-page":"403","DOI":"10.1016\/j.ccm.2017.04.004","article-title":"Clinical Perspectives in the Diagnosis and Management of Histoplasmosis","volume":"38","author":"Azar","year":"2017","journal-title":"Clin. Chest. Med."},{"key":"ref_3","doi-asserted-by":"crossref","unstructured":"Rodrigues, M.L., and Nosanchuk, J.D. (2020). Fungal Diseases as Neglected Pathogens: A Wake-up Call to Public Health Officials. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0007964"},{"key":"ref_4","doi-asserted-by":"crossref","unstructured":"Nargesi, S., Bongomin, F., and Hedayati, M.T. (2021). The Impact of COVID-19 Pandemic on AIDS-Related Mycoses and Fungal Neglected Tropical Diseases: Why Should We Worry?. PLoS Negl. Trop. Dis., 15.","DOI":"10.1371\/journal.pntd.0009092"},{"key":"ref_5","doi-asserted-by":"crossref","unstructured":"Verweij, P.E., and Alanio, A. (2020). Fungal Infections Should Be Part of the Core Outcome Set for COVID-19. Lancet Infect. Dis., online ahead of print.","DOI":"10.1016\/S1473-3099(20)30591-0"},{"key":"ref_6","doi-asserted-by":"crossref","first-page":"100971","DOI":"10.1016\/j.mycmed.2020.100971","article-title":"Invasive Fungal Diseases during COVID-19: We Should Be Prepared","volume":"30","author":"Gangneux","year":"2020","journal-title":"J. Mycol. Med."},{"key":"ref_7","doi-asserted-by":"crossref","unstructured":"de Macedo, P.M., Freitas, D.F.S., Varon, A.G., Lamas, C.d.C., Ferreira, L.C.F., d\u2019Avila Freitas, A., Ferreira, M.T., Nunes, E.P., Siqueira, M.M., and Veloso, V.G. (2020). COVID-19 and Acute Juvenile Paracoccidioidomycosis Coinfection. PLoS Negl. Trop. Dis., 14.","DOI":"10.1371\/journal.pntd.0008559"},{"key":"ref_8","doi-asserted-by":"crossref","unstructured":"Messina, F.A., Marin, E., Caceres, D.H., Romero, M., Depardo, R., Priarone, M.M., Rey, L., V\u00e1zquez, M., Verweij, P.E., and Chiller, T.M. (2020). Coronavirus Disease 2019 (COVID-19) in a Patient with Disseminated Histoplasmosis and HIV\u2014A Case Report from Argentina and Literature Review. J. Fungi, 6.","DOI":"10.3390\/jof6040275"},{"key":"ref_9","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1007\/s11046-020-00505-1","article-title":"COVID-19-Associated Histoplasmosis in an AIDS Patient","volume":"186","author":"Basso","year":"2020","journal-title":"Mycopathologia"},{"key":"ref_10","doi-asserted-by":"crossref","first-page":"1222","DOI":"10.1177\/0956462420957518","article-title":"COVID-19 Associated with AIDS-Related Disseminated Histoplasmosis: A Case Report","volume":"31","author":"Bertolini","year":"2020","journal-title":"Int. J. STD AIDS"},{"key":"ref_11","doi-asserted-by":"crossref","first-page":"53","DOI":"10.1590\/S0074-02761992000100010","article-title":"A Medium for Inducing Conversion of Histoplasma capsulatum Var. Capsulatum into Its Yeast-like Form","volume":"87","author":"Fressatti","year":"1992","journal-title":"Mem. Inst. Oswaldo Cruz"},{"key":"ref_12","doi-asserted-by":"crossref","first-page":"1644","DOI":"10.1128\/JCM.40.5.1644-1647.2002","article-title":"Evaluation of Two Nested PCR Assays for Detection of Histoplasma capsulatum DNA in Human Tissue","volume":"40","author":"Bialek","year":"2002","journal-title":"J. Clin. Microbiol."},{"key":"ref_13","first-page":"1","article-title":"Diffusion-in-Gel Methods for Immunological Analysis","volume":"5","author":"Ouchterlony","year":"1958","journal-title":"Prog. Allergy"},{"key":"ref_14","doi-asserted-by":"crossref","first-page":"131","DOI":"10.1007\/BF00468007","article-title":"Procedures for the Production and Separation of H and M Antigens in Histoplasmin: Chemical and Serological Properties of the Isolated Products","volume":"61","author":"Pine","year":"1977","journal-title":"Mycopathologia"},{"key":"ref_15","doi-asserted-by":"crossref","unstructured":"Almeida, M.d.A., Pizzini, C.V., Damasceno, L.S., Muniz, M.d.M., Almeida-Paes, R., Peralta, R.H.S., Peralta, J.M., Oliveira, R.d.V.C., Vizzoni, A.G., and de Andrade, C.L.T. (2016). Validation of Western Blot for Histoplasma capsulatum Antibody Detection Assay. BMC Infect. Dis., 16.","DOI":"10.1186\/s12879-016-1427-0"},{"key":"ref_16","doi-asserted-by":"crossref","first-page":"390","DOI":"10.1128\/cdli.1.4.390-393.1994","article-title":"Effects of Histoplasmin M Antigen Chemical and Enzymatic Deglycosylation on Cross-Reactivity in the Enzyme-Linked Immunoelectrotransfer Blot Method","volume":"1","author":"Bragg","year":"1994","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"ref_17","first-page":"103","article-title":"Histoplasmosis Presenting as Acute Respiratory Distress Syndrome after Exposure to Bat Feces in a Home Basement","volume":"4","author":"Martins","year":"2000","journal-title":"Braz. J. Infect. Dis."},{"key":"ref_18","doi-asserted-by":"crossref","first-page":"735","DOI":"10.1055\/s-0031-1295721","article-title":"Pulmonary Histoplasmosis","volume":"32","author":"McKinsey","year":"2011","journal-title":"Semin. Respir. Crit. Care Med."},{"key":"ref_19","first-page":"881","article-title":"Histoplasmosis Outbreaks in Brazil: Lessons to Learn About Preventing Exposure","volume":"185","author":"Guerra","year":"2020","journal-title":"Mycopathologia"},{"key":"ref_20","doi-asserted-by":"crossref","first-page":"102863","DOI":"10.1016\/j.msard.2021.102863","article-title":"COVID-19 in a Temporal Relation to the Onset of Multiple Sclerosis","volume":"50","author":"Fragoso","year":"2021","journal-title":"Mult. Scler. Relat. Disord."},{"key":"ref_21","doi-asserted-by":"crossref","first-page":"413","DOI":"10.1038\/s41584-020-0448-7","article-title":"Autoimmune and Inflammatory Diseases Following COVID-19","volume":"16","author":"Galeotti","year":"2020","journal-title":"Nat. Rev. Rheumatol."},{"key":"ref_22","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1186\/s13054-020-02911-9","article-title":"Acute Respiratory Failure in COVID-19: Is It \u201cTypical\u201d ARDS?","volume":"24","author":"Li","year":"2020","journal-title":"Crit. Care"},{"key":"ref_23","doi-asserted-by":"crossref","first-page":"914","DOI":"10.4269\/ajtmh.1999.61.914","article-title":"Environmental Conditions Favoring Bat Infection with Histoplasma capsulatum in Mexican Shelters","volume":"61","author":"Taylor","year":"1999","journal-title":"Am. J. Trop. Med. Hyg."},{"key":"ref_24","doi-asserted-by":"crossref","unstructured":"Da Silva, J.A., Scofield, A., Barros, F.d.N., de Farias, D.M., Riet-Correa, G., Bezerra, P.S., Santos, T.F.S., Tavares, G.S.F., Trevelin, L.C., and da Paz, G.S. (2020). Molecular Detection of Histoplasma capsulatum in Bats of the Amazon Biome in Par\u00e1 State, Brazil. Transbound. Emerg. Dis., online ahead of print.","DOI":"10.1111\/tbed.13740"},{"key":"ref_25","doi-asserted-by":"crossref","first-page":"20","DOI":"10.1128\/CDLI.6.1.20-23.1999","article-title":"Evaluation of a Western Blot Test in an Outbreak of Acute Pulmonary Histoplasmosis","volume":"6","author":"Pizzini","year":"1999","journal-title":"Clin. Diagn. Lab. Immunol."},{"key":"ref_26","doi-asserted-by":"crossref","first-page":"62","DOI":"10.1128\/CVI.00332-09","article-title":"Validation and Clinical Application of a Molecular Method for Identification of Histoplasma capsulatum in Human Specimens in Colombia, South America","volume":"17","author":"Arango","year":"2010","journal-title":"Clin. Vaccine Immunol."},{"key":"ref_27","doi-asserted-by":"crossref","unstructured":"Nacher, M., Blanchet, D., Bongomin, F., Chakrabarti, A., Couppi\u00e9, P., Demar, M., Denning, D.W., Djossou, F., Epelboin, L., and Govender, N. (2018). Histoplasma capsulatum Antigen Detection Tests as an Essential Diagnostic Tool for Patients with Advanced HIV Disease in Low and Middle Income Countries: A Systematic Review of Diagnostic Accuracy Studies. PLoS Negl. Trop. Dis., 12.","DOI":"10.1371\/journal.pntd.0006802"},{"key":"ref_28","doi-asserted-by":"crossref","first-page":"1878","DOI":"10.1086\/648421","article-title":"Diagnosis of Acute Pulmonary Histoplasmosis by Antigen Detection","volume":"49","author":"Swartzentruber","year":"2009","journal-title":"Clin. Infect. Dis."},{"key":"ref_29","doi-asserted-by":"crossref","unstructured":"C\u00e1ceres, D.H., Samayoa, B.E., Medina, N.G., Tob\u00f3n, A.M., Guzm\u00e1n, B.J., Mercado, D., Restrepo, A., Chiller, T., Arathoon, E.E., and G\u00f3mez, B.L. (2018). Multicenter Validation of Commercial Antigenuria Reagents to Diagnose Progressive Disseminated Histoplasmosis in People Living with HIV\/AIDS in Two Latin American Countries. J. Clin. Microbiol., 56.","DOI":"10.1128\/JCM.01959-17"},{"key":"ref_30","doi-asserted-by":"crossref","first-page":"941","DOI":"10.1007\/s10096-007-0380-7","article-title":"Platelia Aspergillus Assay for Diagnosis of Disseminated Histoplasmosis","volume":"26","author":"Ranque","year":"2007","journal-title":"Eur. J. Clin. Microbiol. Infect. Dis."},{"key":"ref_31","doi-asserted-by":"crossref","first-page":"119","DOI":"10.1016\/j.mmcr.2012.10.009","article-title":"Cross-Reactivity of Aspergillus Galactomannan in an HIV-Infected Patient with Histoplasmosis","volume":"1","author":"Min","year":"2012","journal-title":"Med. Mycol. Case Rep."},{"key":"ref_32","doi-asserted-by":"crossref","first-page":"147","DOI":"10.1016\/j.ijid.2019.07.009","article-title":"The Occurrence of Histoplasmosis in Brazil: A Systematic Review","volume":"86","author":"Almeida","year":"2019","journal-title":"Int. J. Infect. Dis."},{"key":"ref_33","doi-asserted-by":"crossref","unstructured":"Werneck, G.L., Porto, L.C., Sena, A., Ferreira, O.d.C., Cavalcanti, A.C., Santos, \u00c2.M.G., Secco, D.A., Silva, M., Mariani, D., and Chieppe, A. (2021). The Incidence and Geographical Spread of SARS-CoV-2 in Rio de Janeiro, Brazil Based on RT-PCR Test Results. Rev. Soc. Bras. Med. Trop., 54.","DOI":"10.1590\/0037-8682-0779-2020"}],"container-title":["Journal of Fungi"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/www.mdpi.com\/2309-608X\/7\/5\/346\/pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,10,11]],"date-time":"2025-10-11T05:54:45Z","timestamp":1760162085000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.mdpi.com\/2309-608X\/7\/5\/346"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,4,28]]},"references-count":33,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2021,5]]}},"alternative-id":["jof7050346"],"URL":"https:\/\/doi.org\/10.3390\/jof7050346","relation":{},"ISSN":["2309-608X"],"issn-type":[{"value":"2309-608X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,4,28]]}}}