{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,9]],"date-time":"2026-09-09T01:49:32Z","timestamp":1788918572647,"version":"build-2803163510"},"reference-count":48,"publisher":"MDPI AG","issue":"7","license":[{"start":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T00:00:00Z","timestamp":1624492800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100006769","name":"Russian Science Foundation","doi-asserted-by":"publisher","award":["19-73-30017"],"award-info":[{"award-number":["19-73-30017"]}],"id":[{"id":"10.13039\/501100006769","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["JoF"],"abstract":"<jats:p>Monoclonal antibody EBCA-1 is used in the sandwich immune assay for the detection of circulating Candida mannan in blood sera samples for the diagnosis of invasive candidiasis. To reinvestigate carbohydrate specificity of EBCA-1, a panel of biotinylated oligosaccharides structurally related to distinct fragments of Candida mannan were loaded onto a streptavidin-coated plate to form a glycoarray. Its use demonstrated that EBCA-1 recognizes the trisaccharide \u03b2-Man-(1\u21922)-\u03b1-Man-(1\u21922)-\u03b1-Man and not homo-\u03b1-(1\u21922)-linked pentamannoside, as was reported previously.<\/jats:p>","DOI":"10.3390\/jof7070504","type":"journal-article","created":{"date-parts":[[2021,6,24]],"date-time":"2021-06-24T11:01:38Z","timestamp":1624532498000},"page":"504","update-policy":"https:\/\/doi.org\/10.3390\/mdpi_crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Reinvestigation of Carbohydrate Specificity of EBCA-1 Monoclonal Antibody Used for the Detection of Candida Mannan"],"prefix":"10.3390","volume":"7","author":[{"given":"Vadim B.","family":"Krylov","sequence":"first","affiliation":[{"name":"Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciencesa, Leninsky Prospect 47, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Arsenii S.","family":"Solovev","sequence":"additional","affiliation":[{"name":"Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciencesa, Leninsky Prospect 47, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ilya A.","family":"Puchkin","sequence":"additional","affiliation":[{"name":"Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciencesa, Leninsky Prospect 47, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dmitry V.","family":"Yashunsky","sequence":"additional","affiliation":[{"name":"Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciencesa, Leninsky Prospect 47, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8074-1890","authenticated-orcid":false,"given":"Anna V.","family":"Antonets","sequence":"additional","affiliation":[{"name":"Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciencesa, Leninsky Prospect 47, 119991 Moscow, Russia"},{"name":"Medical Genetic Center, Rostov-on-Don State Medical University, Nakhichevansky, 29, 344022 Rostov-on-Don, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Olga Y.","family":"Kutsevalova","sequence":"additional","affiliation":[{"name":"National Medical Research Center of Oncology, Laboratory of Clinical Microbiology, 14 Liniya Str., 63, 344037 Rostov-on-Don, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0727-4050","authenticated-orcid":false,"given":"Nikolay E.","family":"Nifantiev","sequence":"additional","affiliation":[{"name":"Laboratory of Glycoconjugate Chemistry, N.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciencesa, Leninsky Prospect 47, 119991 Moscow, Russia"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1968","published-online":{"date-parts":[[2021,6,24]]},"reference":[{"key":"ref_1","doi-asserted-by":"crossref","unstructured":"Bongomin, F., Gago, S., Oladele, R.O., and Denning, D.W. (2017). Global and multi-national prevalence of fungal diseases-estimate precision. J. Fungi, 3.","DOI":"10.3390\/jof3040057"},{"key":"ref_2","doi-asserted-by":"crossref","unstructured":"Demin, K.A., Refeld, A.G., Bogdanova, A.A., Prazdnova, E.V., Popov, I.V., Kutsevalova, O.Y., Ermakov, A.M., Bren, A.B., Rudoy, D.V., and Chistyakov, V.A. (2021). Mechanisms of candida resistance to antimycotics and promising ways to overcome it: The role of probiotics. Probiot. Antimicrob. 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